Plasmids for nicotine-dependent and -independent gene expression in Arthrobacter nicotinovorans and other arthrobacter species.

Abstract:

:The first inducible Arthrobacter overexpression system, based on the promoter/operator and the repressor of the 6-D-hydroxynicotine oxidase gene of Arthrobacter nicotinovorans, is described here. Nicotine-dependent overproduction and affinity purification of recombinant proteins are presented. The system will allow the production of complex enzymes and genetic complementation studies in Arthrobacter species.

journal_name

Appl Environ Microbiol

authors

Sandu C,Chiribau CB,Sachelaru P,Brandsch R

doi

10.1128/AEM.71.12.8920-8924.2005

keywords:

subject

Has Abstract

pub_date

2005-12-01 00:00:00

pages

8920-4

issue

12

eissn

0099-2240

issn

1098-5336

pii

71/12/8920

journal_volume

71

pub_type

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