Curvularia lunata, a New Source of Cytochalasin B.

Abstract:

:A biologically active metabolite was found in crude extracts of Curvularia lunata (Wakker) Boedijn (ATCC 34690) isolated from decayed tissues of litchi fruit (Litchi chinensis Sonn.). The fungus was grown on a shredded wheat-yeast extract-sucrose medium, and cultures were extracted with chloroform after 3 weeks of growth at 21 degrees C. Chloroform extracts were toxic to day-old cockerels and caused abnormal distortion of wheat coleoptile segments. The major toxin had a 50% lethal dose of 700 mg/kg (oral dose) and was a colorless crystalline material with a melting point of 218 degrees C. Elemental and high-resolution mass spectral analyses indicated a formula of C(29)H(37)NO(5) and a molecular weight of 479.62. The crystalline preparation was identified as 97% cytochalasin B and 3% cytochalasin A. The yield of cytochalasin B from C. lunata cultures grown on 4.5 kg of shredded wheat and 9 liters of yeast extract-sucrose medium was 6.24 g of purified material.

journal_name

Appl Environ Microbiol

authors

Wells JM,Cole RJ,Cutler HC,Spalding DH

doi

10.1128/AEM.41.4.967-971.1981

keywords:

subject

Has Abstract

pub_date

1981-04-01 00:00:00

pages

967-71

issue

4

eissn

0099-2240

issn

1098-5336

journal_volume

41

pub_type

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