Abstract:
:A biologically active metabolite was found in crude extracts of Curvularia lunata (Wakker) Boedijn (ATCC 34690) isolated from decayed tissues of litchi fruit (Litchi chinensis Sonn.). The fungus was grown on a shredded wheat-yeast extract-sucrose medium, and cultures were extracted with chloroform after 3 weeks of growth at 21 degrees C. Chloroform extracts were toxic to day-old cockerels and caused abnormal distortion of wheat coleoptile segments. The major toxin had a 50% lethal dose of 700 mg/kg (oral dose) and was a colorless crystalline material with a melting point of 218 degrees C. Elemental and high-resolution mass spectral analyses indicated a formula of C(29)H(37)NO(5) and a molecular weight of 479.62. The crystalline preparation was identified as 97% cytochalasin B and 3% cytochalasin A. The yield of cytochalasin B from C. lunata cultures grown on 4.5 kg of shredded wheat and 9 liters of yeast extract-sucrose medium was 6.24 g of purified material.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Wells JM,Cole RJ,Cutler HC,Spalding DHdoi
10.1128/AEM.41.4.967-971.1981keywords:
subject
Has Abstractpub_date
1981-04-01 00:00:00pages
967-71issue
4eissn
0099-2240issn
1098-5336journal_volume
41pub_type
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