Raman spectroscopy-compatible inactivation method for pathogenic endospores.

abstract：:Water samples from rivers, streams, ponds, and activated sewage were tested for the presence of bacteria which utilize 2,4-dichlorophenoxyacetic acid (2,4-D) as a sole source of carbon. Seventy percent of the attempted enrichments yielded pure cultures of 2,4-D-metabolizing bacteria. All but 1 of the 30 isolates were ...

journal_title：Applied and environmental microbiology

authors： Amy PS,Schulke JW,Frazier LM,Seidler RJ

更新日期：1985-05-01 00:00:00

abstract：:Sakacin K is an antilisterial bacteriocin produced by Lactobacillus sake CTC 494, a strain isolated from Spanish dry fermented sausages. The biokinetics of cell growth and bacteriocin production of L. sake CTC 494 in vitro during laboratory fermentations were investigated by making use of MRS broth. The data obtained ...

journal_title：Applied and environmental microbiology

authors： Leroy F,de Vuyst L

更新日期：1999-03-01 00:00:00

abstract：:Xylanase activity from naturally occurring color variants of Aureobasidium pullulans was associated with extracellular monomeric proteins of 20 to 21 kilodaltons. Xylanase represented nearly half the total extracellular protein, with a yield of up to 0.3 g of xylanase per liter. The specific activity of partially puri...

journal_title：Applied and environmental microbiology

authors： Leathers TD

更新日期：1986-11-01 00:00:00

abstract：:We report an ex vivo method, which uses pieces of human skin excised during routine plastic surgery, for testing survival of hazardous pathogens. Using this procedure, we compared the survival of human herpesvirus 2 on human skin and on metal disks. At the physiological skin temperature of 32 degrees C, the half-life ...

journal_title：Applied and environmental microbiology

authors： Graham ML,Springthorpe VS,Sattar SA

更新日期：1996-11-01 00:00:00

abstract：:The effects of polychlorinated biphenyl (PCB) and phenanthrene stress on glucose uptake by natural microbial populations were examined by the heterotrophic potential technique. Temporal and spatial distributions in glucose uptake velocities were examined for natural samples as well as PCB- and phenanthrene-stressed sa...

journal_title：Applied and environmental microbiology

authors： Sayler GS,Lund LC,Shiaris MP,Sherrill TW,Perkins RE

更新日期：1979-05-01 00:00:00

abstract：:Storage of Salmonella enterica serovar Typhimurium strains in soil and water microcosms resulted in loss of culturability on standard plating media. Prior incubation in buffered peptone water supplemented with ferrioxamine E markedly extended the time that bacteria were recoverable by plating, except in the case of mu...

journal_title：Applied and environmental microbiology

authors： Reissbrodt R,Heier H,Tschäpe H,Kingsley RA,Williams PH

更新日期：2000-09-01 00:00:00

abstract：:Four soil temperature and moisture treatment regimens were imposed on Florunner peanuts 94 days after planting in experimental plots in 1980. At harvest (145 days after planting), the incidence of the Aspergillus flavus group and the aflatoxin concentration were greatest in damaged kernels. Extensive colonization of s...

journal_title：Applied and environmental microbiology

authors： Hill RA,Blankenship PD,Cole RJ,Sanders TH

更新日期：1983-02-01 00:00:00

abstract：:We sequenced the Staphylococcus aureus phospho-beta-galactosidase gene. The protein product of this gene consisted of 470 amino acids, giving a molecular weight of 54,557. This gene appears to be transcribed as the terminal sequence on a polycistronic message. ...

journal_title：Applied and environmental microbiology

authors： Breidt F Jr,Stewart GC

更新日期：1987-05-01 00:00:00

abstract：:Mesophilic and psychrotrophic populations from refrigerated meat were identified in this study, and the spoilage potential of microbial isolates in packaged beef was evaluated by analyzing the release of volatile organic compounds (VOC) by gas chromatography-mass spectrometry (GC/MS). Fifty mesophilic and twenty-nine ...

journal_title：Applied and environmental microbiology

authors： Ercolini D,Russo F,Nasi A,Ferranti P,Villani F

更新日期：2009-04-01 00:00:00

abstract：:Bifidobacteria are a major component of the intestinal microbiota in humans, particularly breast-fed infants. Therefore, elucidation of the mechanisms by which these bacteria colonize the intestine is desired. One approach is transposon mutagenesis, a technique currently attracting much attention because, in combinati...

journal_title：Applied and environmental microbiology

authors： Sakanaka M,Nakakawaji S,Nakajima S,Fukiya S,Abe A,Saburi W,Mori H,Yokota A

更新日期：2018-08-17 00:00:00

abstract：:The role of three key nitrogen regulatory genes, glnB (encoding the P(II) protein), glnZ (encoding the P(z) protein), and glnD (encoding the GlnD protein), in regulation of poly-3-hydroxybutyrate (PHB) biosynthesis by ammonia in Azospirillum brasilense Sp7 was investigated. It was observed that glnB glnZ and glnD muta...

journal_title：Applied and environmental microbiology

authors： Sun J,Van Dommelen A,Van Impe J,Vanderleyden J

更新日期：2002-02-01 00:00:00

abstract：:Small-subunit ribosomal DNA (SSU rDNA) from 20 phenotypically distinct strains of 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading bacteria was partially sequenced, yielding 18 unique strains belonging to members of the alpha, beta, and gamma subgroups of the class Proteobacteria. To understand the origin of 2,4-D deg...

journal_title：Applied and environmental microbiology

authors： McGowan C,Fulthorpe R,Wright A,Tiedje JM

更新日期：1998-10-01 00:00:00

abstract：:Homologous recombination plays an important role in the structuring of genetic variation of many bacteria; however, its importance in adaptive evolution is not well established. We investigated the association of intersubspecific homologous recombination (IHR) with the shift to a novel host (mulberry) by the plant-pat...

journal_title：Applied and environmental microbiology

authors： Nunney L,Schuenzel EL,Scally M,Bromley RE,Stouthamer R

更新日期：2014-05-01 00:00:00

abstract：:Two-component regulatory systems are one primary mechanism for environmental sensing and signal transduction. Annotation of the complete genome sequence of the probiotic bacterium Lactobacillus acidophilus NCFM revealed nine two-component regulatory systems. In this study, the histidine protein kinase of a two-compone...

journal_title：Applied and environmental microbiology

authors： Azcarate-Peril MA,McAuliffe O,Altermann E,Lick S,Russell WM,Klaenhammer TR

更新日期：2005-10-01 00:00:00

abstract：:Tropomyosin receptor kinase A (TrkA), a receptor tyrosine kinase, is known to be associated with various diseases. Thus, TrkA has become a major drug-screening target for these diseases. Despite the fact that the production of recombinant proteins by prokaryotic hosts has advantages, such as fast growth and ease of ge...

journal_title：Applied and environmental microbiology

authors： Honda T,Yasuda T,Tanaka T,Hagiwara K,Arai T,Yoshino T

更新日期：2015-02-01 00:00:00

abstract：:Pectinolytic enzymes play an important role in cocoa fermentation. In this study, we characterized three extracellular pectate lyases (Pels) produced by bacilli isolated from fermenting cocoa beans. These enzymes, named Pel-22, Pel-66, and Pel-90, were synthesized by Bacillus pumilus BS22, Bacillus subtilis BS66, and ...

journal_title：Applied and environmental microbiology

authors： Ouattara HG,Reverchon S,Niamke SL,Nasser W

更新日期：2010-08-01 00:00:00

abstract：:Accurate predictions of carbon and energy cycling rates in the environment depend on sampling frequencies and on the spatial variability associated with biological activities. We examined the variability associated with anaerobic biodegradation rates at two sites in an alluvial sand aquifer polluted by municipal landf...

journal_title：Applied and environmental microbiology

authors： Adrian NR,Robinson JA,Suflita JM

更新日期：1994-10-01 00:00:00

abstract：:Blooms of the cyanobacterium Anabaena circinalis are a major worldwide problem due to their production of a range of toxins, in particular the neurotoxins anatoxin-a and paralytic shellfish poisons (PSPs). Although there is a worldwide distribution of A. circinalis, there is a geographical segregation of neurotoxin pr...

journal_title：Applied and environmental microbiology

authors： Beltran EC,Neilan BA

更新日期：2000-10-01 00:00:00

abstract：:Four membrane filter methods for the enumeration of fecal coliforms were compared for accuracy, specificity, and recovery. Water samples were taken several times from 13 marine, 1 estuarine, and 4 freshwater sites around Puerto Rico, from pristine waters and waters receiving treated and untreated sewage and effluent f...

journal_title：Applied and environmental microbiology

authors： Santiago-Mercado J,Hazen TC

更新日期：1987-12-01 00:00:00

abstract：:An epoxyalkane:coenzyme M (CoM) transferase (EaCoMT) enzyme was recently found to be active in the aerobic vinyl chloride (VC) and ethene assimilation pathways of Mycobacterium strain JS60. In the present study, EaCoMT activity and genes were investigated in 10 different mycobacteria isolated on VC or ethene from dive...

journal_title：Applied and environmental microbiology

authors： Coleman NV,Spain JC

更新日期：2003-10-01 00:00:00

abstract：:During malolactic fermentation in wine by Oenococcus oeni, the degradation of citric acid was delayed compared to the degradation of malic acid. The maximum concentration of diacetyl, an intermediary compound in the citric acid metabolism with a buttery or nutty flavor, coincided with the exhaustion of malic acid in t...

journal_title：Applied and environmental microbiology

authors： Nielsen JC,Richelieu M

更新日期：1999-02-01 00:00:00

abstract：:Fusarium graminearum is an important fungal pathogen of cereal crops and produces mycotoxins, such as the trichothecenes nivalenol and deoxynivalenol. This species may be subdivided into a series of genetic lineages or phylogenetic species. We identified strains of F. graminearum from the Republic of Korea to lineage,...

journal_title：Applied and environmental microbiology

authors： Lee J,Chang IY,Kim H,Yun SH,Leslie JF,Lee YW

更新日期：2009-05-01 00:00:00

abstract：:Campylobacter jejuni isolates possess multiple adhesive proteins termed adhesins, which promote the organism's attachment to epithelial cells. Based on the proposal that one or more adhesins are shared among C. jejuni isolates, we hypothesized that C. jejuni strains would compete for intestinal and cecal colonization ...

journal_title：Applied and environmental microbiology

authors： Konkel ME,Christensen JE,Dhillon AS,Lane AB,Hare-Sanford R,Schaberg DM,Larson CL

更新日期：2007-04-01 00:00:00

abstract：:Populations of Rhizobium leguminosarum biovar viciae were sampled from two bulk soils, rhizosphere, and nodules of host legumes, fava bean (Vicia faba) and pea (Pisum sativum) grown in the same soils. Additional populations nodulating peas, fava beans, and vetches (Vicia sativa) grown in other soils and fava bean-nodu...

journal_title：Applied and environmental microbiology

authors： Laguerre G,Louvrier P,Allard MR,Amarger N

更新日期：2003-04-01 00:00:00

abstract：:Total coliforms and total coliforms resistant to streptomycin, tetracycline, or chloramphenicol were isolated from filtered activated sludge effluents before and after UV light irradiation. Although the UV irradiation effectively disinfected the wastewater effluent, the percentage of the total surviving coliform popul...

journal_title：Applied and environmental microbiology

authors： Meckes MC

更新日期：1982-02-01 00:00:00

abstract：:An unknown member of the family Pasteurellaceae was repeatedly isolated from 20- to 24-week-old pigs with severe pulmonary lesions reared on the same farm in Victoria, Australia. The etiological diagnosis of the disease was inconclusive. The complete genome sequence analysis of one strain, 15-184, revealed some phylog...

journal_title：Applied and environmental microbiology

authors： Watt AE,Browning GF,Legione AR,Bushell RN,Stent A,Cutler RS,Young ND,Marenda MS

更新日期：2018-05-17 00:00:00

abstract：:Ceftiofur, a third-generation cephalosporin antimicrobial, was used in Canadian hatcheries for many years to prevent early mortality in chicks, leading to a high prevalence of cephalosporin resistance in Escherichia coli in chickens. Preventive use of ceftiofur in hatcheries ceased in 2014. We examined the effect of c...

journal_title：Applied and environmental microbiology

authors： Verrette L,Fairbrother JM,Boulianne M

更新日期：2019-06-17 00:00:00

abstract：:The quantification of 16S rRNA by oligonucleotide probe hybridization was investigated with MagnaGraph (Micron Separation, Inc. [MSI]), Magna Charge (MSI), Magna (MSI), Immobilon-N (Millipore Corporation), and Nytran (Schleicher & Schuell, Inc.) membranes as supports for nucleic acid immobilization. The levels of dete...

journal_title：Applied and environmental microbiology

authors： Raskin L,Capman WC,Kane MD,Rittmann BE,Stahl DA

更新日期：1996-01-01 00:00:00

abstract：:The diagnostically important surface antigen pre-S2 of hepatitis B virus was produced in large amounts in the periplasmic space of Escherichia coli. The DNA fragments (pre-S2) coding the pre-S2 antigen were tandemly duplicated or triplicated and ligated in the same reading frame to a fragment containing the promoter a...

journal_title：Applied and environmental microbiology

authors： Kadokura H,Yoda K,Imai M,Yamasaki M

更新日期：1990-09-01 00:00:00

abstract：:Mechanisms for Fe(III) oxide reduction were investigated in Geothrix fermentans, a dissimilatory Fe(III)-reducing microorganism found within the Fe(III) reduction zone of subsurface environments. Culture filtrates of G. fermentans stimulated the reduction of poorly crystalline Fe(III) oxide by washed cell suspensions,...

journal_title：Applied and environmental microbiology

authors： Nevin KP,Lovley DR

更新日期：2002-05-01 00:00:00