Abstract:
:The diagnostically important surface antigen pre-S2 of hepatitis B virus was produced in large amounts in the periplasmic space of Escherichia coli. The DNA fragments (pre-S2) coding the pre-S2 antigen were tandemly duplicated or triplicated and ligated in the same reading frame to a fragment containing the promoter and the signal sequence of the alkaline phosphatase-coding gene (phoA) of E. coli. Further, a DNA fragment (bla) coding mature beta-lactamase was joined to the region coding the C terminus of the pre-S2 repeat to stabilize the gene product. Upon induction of the phoA-(pre-S2)3-bla fusion gene, the fusion protein was produced at up to 30% of the total cellular protein. Fractionation of the cellular components and trypsin accessibility of the product showed that the antigen was secreted in the periplasm and formed inclusion bodies there. The signal sequence of alkaline phosphatase was found to be correctly processed in E. coli.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Kadokura H,Yoda K,Imai M,Yamasaki Mdoi
10.1128/AEM.56.9.2742-2747.1990subject
Has Abstractpub_date
1990-09-01 00:00:00pages
2742-7issue
9eissn
0099-2240issn
1098-5336journal_volume
56pub_type
杂志文章abstract::Differing incidences of Vibrio parahaemolyticus reported by separate government agencies are attributable to sample handling and subsequent isolation techniques. ...
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.60.7.2545-2552.1994
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