Effect of reactor turbulence on the binding-protein-mediated aspartate transport system in thin wastewater biofilms.

Abstract:

:This research documents an effect of reactor turbulence on the ability of gram-negative wastewater biofilm bacteria to actively transport l-aspartate via a binding-protein-mediated transport system. Biofilms which were not preadapted to turbulence and which possessed two separate and distinct aspartate transport systems (systems 1 and 2) were subjected to a turbulent flow condition in a hydrodynamically defined closed-loop reactor system. A shear stress treatment of 3.1 N . m for 10 min at a turbulent Reynolds number (Re = 11,297) inactivated the low-affinity, high-capacity binding-protein-mediated transport system (system 2) and resolved the high-affinity, low-capacity membrane-bound proton symport system (system 1). The K(t) and V(max) values for the resolved system were statistically similar to K(t) and V(max) values for system 1 when system 2 was inactivated either by osmotic shock or arsenate, two treatments which are known to inactivate binding-protein-mediated transport systems. We hypothesize that shear stress disrupts system 2 by deforming the outer membranes of the firmly adhered gram-negative bacteria.

journal_name

Appl Environ Microbiol

authors

Eighmy TT,Bishop PL

doi

10.1128/AEM.50.1.120-124.1985

keywords:

subject

Has Abstract

pub_date

1985-07-01 00:00:00

pages

120-4

issue

1

eissn

0099-2240

issn

1098-5336

journal_volume

50

pub_type

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