Abstract:
:This research documents an effect of reactor turbulence on the ability of gram-negative wastewater biofilm bacteria to actively transport l-aspartate via a binding-protein-mediated transport system. Biofilms which were not preadapted to turbulence and which possessed two separate and distinct aspartate transport systems (systems 1 and 2) were subjected to a turbulent flow condition in a hydrodynamically defined closed-loop reactor system. A shear stress treatment of 3.1 N . m for 10 min at a turbulent Reynolds number (Re = 11,297) inactivated the low-affinity, high-capacity binding-protein-mediated transport system (system 2) and resolved the high-affinity, low-capacity membrane-bound proton symport system (system 1). The K(t) and V(max) values for the resolved system were statistically similar to K(t) and V(max) values for system 1 when system 2 was inactivated either by osmotic shock or arsenate, two treatments which are known to inactivate binding-protein-mediated transport systems. We hypothesize that shear stress disrupts system 2 by deforming the outer membranes of the firmly adhered gram-negative bacteria.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Eighmy TT,Bishop PLdoi
10.1128/AEM.50.1.120-124.1985keywords:
subject
Has Abstractpub_date
1985-07-01 00:00:00pages
120-4issue
1eissn
0099-2240issn
1098-5336journal_volume
50pub_type
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