High-frequency rugose exopolysaccharide production by Vibrio cholerae.

abstract：:Members of a group of marine bacteria that is numerically important in coastal seawater and sediments were characterized with respect to their ability to transform organic and inorganic sulfur compounds. Fifteen strains representing the Roseobacter group (a phylogenetic cluster of marine bacteria in the alpha-subclass...

journal_title：Applied and environmental microbiology

authors： González JM,Kiene RP,Moran MA

更新日期：1999-09-01 00:00:00

abstract：:We detected and identified genotypes of human-pathogenic microsporidia in fecal samples from 51 asymptomatic captive-bred pet parrots in South Korea. Microsporidia were identified in 8 samples (15.7%); 7 parrots tested positive for Encephalitozoon hellem, and 1 parrot tested positive for both E. hellem and Encephalito...

journal_title：Applied and environmental microbiology

authors： Lee SY,Lee SS,Lyoo YS,Park HM

更新日期：2011-12-01 00:00:00

abstract：:Cell extracts of Trichoderma reesei exhibited dibasic endopeptidase activity toward the carboxylic side of KR, RR, and PR sequences. This activity was stimulated by the presence of Ca2+ ions and localized in vesicles of low bouyant density; it therefore exhibited some similarity to yeast Kex2. Analytical chromatofocus...

journal_title：Applied and environmental microbiology

authors： Goller SP,Schoisswohl D,Baron M,Parriche M,Kubicek CP

更新日期：1998-09-01 00:00:00

abstract：:Selective adhesion to only certain epithelia is particularly common among the bacterial members of the indigenous microflora of mammals. We have found that the stratified squamous epithelium of the nonsecreting area of horse stomach is colonized by gram-positive rods. The microscopic features of a dense layer of these...

journal_title：Applied and environmental microbiology

authors： Yuki N,Shimazaki T,Kushiro A,Watanabe K,Uchida K,Yuyama T,Morotomi M

更新日期：2000-11-01 00:00:00

abstract：:An indirect fluorescent antibody-membrane filter staining technique, which permitted the autecological study of Methylomonas methanica and Methylosinus trichosporium, was developed. This technique was used to assay the numbers of these two organisms in Cleveland Harbor. The concentrations of M. methanica and M. tricho...

journal_title：Applied and environmental microbiology

authors： Reed WM,Dugan PR

更新日期：1978-02-01 00:00:00

abstract：:Several wild-type isolates of marine bdellovibrios formed stable bdelloplasts when they infected gram-negative bacterial prey under certain culture conditions. Synchronous predator-prey cultures and low nutrient concentrations increased the yield of stable bdelloplasts. The bdellovibrio cells retained in the stable bd...

journal_title：Applied and environmental microbiology

authors： Sánchez-Amat A,Torrella F

更新日期：1990-09-01 00:00:00

abstract：:A nested single-copy locus-based quantitative PCR (qPCR) assay and a multicopy locus-based qPCR assay were developed to estimate endophytic biomass of fungal root symbionts belonging to the Phialocephala fortinii sensu lato-Acephala applanata species complex (PAC). Both assays were suitable for estimation of endophyti...

journal_title：Applied and environmental microbiology

authors： Tellenbach C,Grünig CR,Sieber TN

更新日期：2010-09-01 00:00:00

abstract：:Three different strains of bacteria isolated from spoiled, uncooked chicken were grown in pure culture on Trypticase soy agar supplemented with yeast extract. The volatile organic compounds produced by each culture were concentrated on a porous polymer precolumn and analyzed by high-resolution gas chromatographic mass...

journal_title：Applied and environmental microbiology

authors： Lee ML,Smith DL,Freeman LR

更新日期：1979-01-01 00:00:00

abstract：:The only major structural protein (35 kDa) of the lactococcal small isometric-headed bacteriophage ul36, a member of the P335 species, was isolated from a preparative sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. Monoclonal antibodies (MAbs) were raised against the denatured 35-kDa protein. Six MAbs...

journal_title：Applied and environmental microbiology

authors： Moineau S,Bernier D,Jobin M,Hébert J,Klaenhammer TR,Pandian S

更新日期：1993-07-01 00:00:00

abstract：:So far, only scarce and rather diffuse information is available on bacteriophages infecting members of the genus Bifidobacterium. In the current study, we investigated the genetic organization, phylogenetic relationships, and, in some cases, transcription profiles and inducibility of 19 prophage-like elements present ...

journal_title：Applied and environmental microbiology

authors： Ventura M,Turroni F,Lima-Mendez G,Foroni E,Zomer A,Duranti S,Giubellini V,Bottacini F,Horvath P,Barrangou R,Sela DA,Mills DA,van Sinderen D

更新日期：2009-11-01 00:00:00

abstract：:It is now possible to obtain accurate total counts of the bacteria of natural waters with the use of acridine orange staining and epifluorescence microscopy. This approach can be coupled to highly sensitive measurements of heterotrophic activity using radioisotopes. To accomplish this, three variations of a "specific ...

journal_title：Applied and environmental microbiology

authors： Wright RT

更新日期：1978-08-01 00:00:00

abstract：:The utilization by Alcaligenes faecalis of electrodes as the electron donor for denitrification was investigated in this study. The denitrification rate of A. faecalis with a poised potential was greatly enhanced compared with that of the controls without poised potentials. For nitrate reduction, although A. faecalis ...

journal_title：Applied and environmental microbiology

authors： Wang X,Yu P,Zeng C,Ding H,Li Y,Wang C,Lu A

更新日期：2015-08-15 00:00:00

abstract：:The killing of Listeria monocytogenes following exposure to low pH, organic acids, and osmotic stress was enhanced by the addition of 5% (vol/vol) ethanol. At pH 3, for example, the presence of this agent stimulated killing by more than 3 log units in 40 min of exposure. The rate of cell death at pH 3.0 was dependent ...

journal_title：Applied and environmental microbiology

authors： Barker C,Park SF

更新日期：2001-04-01 00:00:00

abstract：:The aim of this study was to compare the antagonistic properties of Lactobacillus casei GG exerted in vitro against Salmonella typhimurium C5 in a cellular model, cultured enterocyte-like Caco-2 cells, to those exerted in vivo in an animal model, C3H/He/Oujco mice. Our results show that a 1-h contact between the invad...

journal_title：Applied and environmental microbiology

authors： Hudault S,Liévin V,Bernet-Camard MF,Servin AL

更新日期：1997-02-01 00:00:00

abstract：:In a medium containing ammonia, proteose peptone, and cysteine as nitrogen sources, 17 of 24 Bacteroidaceae strains, 3 of Selenomonas strains, 1 of 7 curved rods, 3 of 7 Spirochaetaceae strains, 8 of 20 Eubacterium strains, 8 of 13 Peptococcaceae strains, 3 of 4 Clostridium strains, 19 of 20 Enterobacteriaceae strains...

journal_title：Applied and environmental microbiology

authors： Takahashi M,Benno Y,Mitsuoka T

更新日期：1980-01-01 00:00:00

abstract：:A dissimilatory Fe(III)- and Mn(IV)-reducing bacterium was isolated from bottom sediments of the Great Bay estuary, New Hampshire. The isolate was a facultatively anaerobic gram-negative rod which did not appear to fit into any previously described genus. It was temporarily designated strain BrY. BrY grew anaerobicall...

journal_title：Applied and environmental microbiology

authors： Caccavo F,Blakemore RP,Lovley DR

更新日期：1992-10-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) has been used to identify bacteria based upon protein signatures. This research shows that while some different proteins are produced by vegetative bacteria when they are cultured in different growth media, positive identificat...

journal_title：Applied and environmental microbiology

authors： Valentine N,Wunschel S,Wunschel D,Petersen C,Wahl K

更新日期：2005-01-01 00:00:00

abstract：:Cellulase and xylanase cDNAs were isolated from a cDNA library of the polycentric anaerobic fungus Orpinomyces sp. strain PC-2 constructed in Escherichia coli. The cellulase cDNA (celB) was 1.8 kb long with an open reading frame (ORF) coding for a polypeptide of 471 amino acids, and the xylanase cDNA (xynA) was 1.2 kb...

journal_title：Applied and environmental microbiology

authors： Li XL,Chen H,Ljungdahl LG

更新日期：1997-02-01 00:00:00

abstract：:A pair of vectors for expression of heterologous genes in Lactococcus lactis was constructed. In addition to an origin of replication that has a broad host range, these vectors contain a multiple cloning site flanked by gene expression signals originating from L. lactis subsp. cremoris Wg2. The two vectors, about 3.7 ...

journal_title：Applied and environmental microbiology

authors： van de Guchte M,van der Vossen JM,Kok J,Venema G

更新日期：1989-01-01 00:00:00

abstract：:A bacterial strain, designated KSM-7358, that can use alpha-cedrene for growth was isolated. The strain was identified as a member of the genus Rhodococcus and catalyzed the novel allylic oxidation of alpha-cedrene regiospecifically to produce (R)-10-hydroxycedrene (sec-cedrenol) with a very high yield. alpha-Curcumen...

journal_title：Applied and environmental microbiology

authors： Takigawa H,Kubota H,Sonohara H,Okuda M,Tanaka S,Fujikura Y,Ito S

更新日期：1993-05-01 00:00:00

abstract：:Gibberella moniliformis (Fusarium verticillioides) is primarily a pathogen of maize, but it can also cause disease in other crop species. This pathogenicity, as well as the contamination of food- and feedstuffs with the fumonisin mycotoxins, results in economically significant losses to both farmers and food processor...

journal_title：Applied and environmental microbiology

authors： Jurgenson JE,Zeller KA,Leslie JF

更新日期：2002-04-01 00:00:00

abstract：:The ability of yeasts to ferment cellodextrins is rare. Candida wickerhamii is able to use these sugars for alcohol production because of a cell-bound, extracellular, beta-glucosidase that is unusual by not being inhibited by glucose. A cDNA expression library in lambda phage was prepared with mRNA isolated from cello...

journal_title：Applied and environmental microbiology

authors： Skory CD,Freer SN

更新日期：1995-02-01 00:00:00

abstract：:Short peptides of 11 residues were synthesized and tested against the economically important plant pathogenic bacteria Erwinia amylovora, Pseudomonas syringae, and Xanthomonas vesicatoria and compared to the previously described peptide Pep3 (WKLFKKILKVL-NH(2)). The antimicrobial activity of Pep3 and 22 analogues was ...

journal_title：Applied and environmental microbiology

authors： Ferre R,Badosa E,Feliu L,Planas M,Montesinos E,Bardají E

更新日期：2006-05-01 00:00:00

abstract：:Two Aeromonas hydrophila bacteriophages, Aeh1 and Aeh2, were isolated from sewage. Both phages showed binal symmetry. The dimensions of A. hydrophila phages Aeh1 and Aeh2 differed from those of the other Aeromonas phages. Also, phage Aeh2 was the largest Aeromonas phage studied to date. Phage Aeh1 formed small, clear ...

journal_title：Applied and environmental microbiology

authors： Chow MS,Rouf MA

更新日期：1983-05-01 00:00:00

abstract：:In this study, we compared different methods-including transmission electron microscopy-and various nucleic acid labeling methods in which we used the fluorochromes 4',6'-diamidino-2-phenylindole (DAPI), 4-[3-methyl-2,3-dihydro-(benzo-1, 3-oxazole)-2-methylmethyledene]-1-(3'-trimethyl ammoniumpropyl)-quinilinium diioi...

journal_title：Applied and environmental microbiology

authors： Bettarel Y,Sime-Ngando T,Amblard C,Laveran H

更新日期：2000-06-01 00:00:00

abstract：:Some Listeria monocytogenes internalins are recognized as contributing to invasion of mammalian tissue culture cells. While PrfA is well established as a positive regulator of L. monocytogenes virulence gene expression, the stress-responsive sigma(B) has been recognized only recently as contributing to expression of v...

journal_title：Applied and environmental microbiology

authors： McGann P,Wiedmann M,Boor KJ

更新日期：2007-05-01 00:00:00

abstract：:Helix alpha 4 of Bacillus thuringiensis Cry toxins is thought to play a critical role in the toxins' mode of action. Accordingly, single-site substitutions of many Cry1Aa helix alpha 4 amino acid residues have previously been shown to cause substantial reductions in the protein's pore-forming activity. Changes in prot...

journal_title：Applied and environmental microbiology

authors： Girard F,Vachon V,Préfontaine G,Marceau L,Schwartz JL,Masson L,Laprade R

更新日期：2009-01-01 00:00:00

abstract：:We recently showed that expressing an H(2)O-NADH oxidase in Saccharomyces cerevisiae drastically reduces the intracellular NADH concentration and substantially alters the distribution of metabolic fluxes in the cell. Although the engineered strain produces a reduced amount of ethanol, a high level of acetaldehyde accu...

journal_title：Applied and environmental microbiology

authors： Heux S,Sablayrolles JM,Cachon R,Dequin S

更新日期：2006-09-01 00:00:00

abstract：:Mesophilic and psychrotrophic populations from refrigerated meat were identified in this study, and the spoilage potential of microbial isolates in packaged beef was evaluated by analyzing the release of volatile organic compounds (VOC) by gas chromatography-mass spectrometry (GC/MS). Fifty mesophilic and twenty-nine ...

journal_title：Applied and environmental microbiology

authors： Ercolini D,Russo F,Nasi A,Ferranti P,Villani F

更新日期：2009-04-01 00:00:00

abstract：:Two gram-positive, strictly anoxic, coccoid- to rod-shaped strains of bacteria, Clostridium coccoides 1410 and C. coccoides 3110, were isolated from human feces on the typical homoacetogenic substrates formate plus H2 plus CO2 (strain 1410) and vanillate plus H2 plus CO2 (strain 3110) in the presence of 2-bromoethanes...

journal_title：Applied and environmental microbiology

authors： Kamlage B,Gruhl B,Blaut M

更新日期：1997-05-01 00:00:00