Abstract:
:Two Aeromonas hydrophila bacteriophages, Aeh1 and Aeh2, were isolated from sewage. Both phages showed binal symmetry. The dimensions of A. hydrophila phages Aeh1 and Aeh2 differed from those of the other Aeromonas phages. Also, phage Aeh2 was the largest Aeromonas phage studied to date. Phage Aeh1 formed small, clear plaques, and phage Aeh2 formed turbid plaques with clear centers. Both phages were sensitive to chloroform treatment, being totally inactivated after treatment for 1 h at 60 degrees C at pH 3 and 11. However, the infectivity of Aeh1 phage stocks increased by approximately fivefold after they were treated at pH 10 for 1 h at 22 degrees C. Phages Aeh1 and Aeh2 were serologically unrelated and had latent periods of 39 and 52 min, respectively. The average burst sizes of phages Aeh1 and Aeh2 were 17 and 92 PFU per cell, respectively. Phage Aeh1 infected 13 of 22 A. hydrophila strains tested, whereas phage Aeh2 infected only its original host. Phage Aeh1 infected some A. hydrophila strains only at or below 37 degrees C. Neither phage infected the two A. (Plesiomonas) shigelloides strains used in this study.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Chow MS,Rouf MAdoi
10.1128/AEM.45.5.1670-1676.1983keywords:
subject
Has Abstractpub_date
1983-05-01 00:00:00pages
1670-6issue
5eissn
0099-2240issn
1098-5336journal_volume
45pub_type
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