PbaR, an IclR family transcriptional activator for the regulation of the 3-phenoxybenzoate 1',2'-dioxygenase gene cluster in Sphingobium wenxiniae JZ-1T.

abstract：:A method was developed for extraction of DNA from Chroococcidiopsis that overcomes obstacles posed by bacterial contamination and the presence of a thick envelope surrounding the cyanobacterial cells. The method is based on the resistance of Chroococcidiopsis to lysozyme and consists of a lysozyme treatment followed b...

journal_title：Applied and environmental microbiology

authors： Billi D,Grilli Caiola M,Paolozzi L,Ghelardini P

更新日期：1998-10-01 00:00:00

abstract：:Enterotoxin B produced by Staphylococus aureus 243 in brain heart infusion broth was concentrated by dialysis against 40% polyethylene glycol (20 M), partially purified on a Sephadex G-100 column and heated at 110 degrees C in thermal death time cans. Various heating menstrua included 0.04 M Veronal buffer (pH 7.4), b...

journal_title：Applied and environmental microbiology

authors： Lee IC,Stevenson KE,Harmon LG

更新日期：1977-02-01 00:00:00

abstract：:We studied injury of Escherichia coli O157:H7 cells in 11 food items during freeze storage and methods of isolating freeze-injured E. coli O157:H7 cells from foods. Food samples inoculated with E. coli O157:H7 were stored for 16 weeks at -20 degrees C in a freezer. Noninjured and injured cells were counted by using tr...

journal_title：Applied and environmental microbiology

authors： Hara-Kudo Y,Ikedo M,Kodaka H,Nakagawa H,Goto K,Masuda T,Konuma H,Kojima T,Kumagai S

更新日期：2000-07-01 00:00:00

abstract：:Ralstonia solanacearum, a major phytopathogenic bacterium, causes a bacterial wilt disease in diverse plants. Although fatty acid analyses of total membranes of R. solanacearum showed that they contain primarily palmitic (C(16:0)), palmitoleic (C(16:1)) and cis-vaccenic (C(18:1)) acids, little is known regarding R. so...

journal_title：Applied and environmental microbiology

authors： Cheng J,Ma J,Lin J,Fan ZC,Cronan JE,Wang H

更新日期：2012-03-01 00:00:00

abstract：:A quantitative real-time PCR targeting the tnaA gene was studied to detect Escherichia coli and distinguish E. coli from Shigella spp. These microorganisms revealed high similarity in the molecular organization of the tna operon. ...

journal_title：Applied and environmental microbiology

authors： Bernasconi C,Volponi G,Picozzi C,Foschino R

更新日期：2007-10-01 00:00:00

abstract：:SAR11 bacteria are small, heterotrophic, marine alphaproteobacteria found throughout the oceans. They thrive at the low nutrient concentrations typical of open ocean conditions, although the adaptations required for life under those conditions are not well understood. To illuminate this issue, we used cryo-electron to...

journal_title：Applied and environmental microbiology

authors： Zhao X,Schwartz CL,Pierson J,Giovannoni SJ,McIntosh JR,Nicastro D

更新日期：2017-01-17 00:00:00

abstract：:Mongolian gerbils were used as an animal model to excyst and host Giardia spp. isolated from meadow voles, dogs, beavers, and humans. Both cysts and trophozoites were used to establish infections. Gerbils were infected with Giardia duodenalis from beaver, dog, and human sources, and the trophozoites were extracted and...

journal_title：Applied and environmental microbiology

authors： Wallis PM,Wallis HM

更新日期：1986-03-01 00:00:00

abstract：:Mycoplasma canadense and Mycoplasma verecundum were cultured in a serum-free medium containing bovine serum albumin, cholesterol, oleic acid, and palmitic acid in order to avoid the addition of horse serum. Growth was detected by measurement of A640 and by colony formation. The level of growth attained in this medium ...

journal_title：Applied and environmental microbiology

authors： Muñoz G,Sotomayor P

更新日期：1990-07-01 00:00:00

abstract：:The frequency of Escherichia coli infection has lead to concerns over pathogenic bacteria in our food supply and a demand for therapeutics. Glycolipids on gut cells serve as receptors for the Shiga-like toxin produced by E. coli. Oligosaccharide moiety analogues of these glycolipids can compete with receptors for the ...

journal_title：Applied and environmental microbiology

authors： Liu Z,Lu Y,Zhang J,Pardee K,Wang PG

更新日期：2003-04-01 00:00:00

abstract：:With an initial microbial level of ca. 10 microorganisms per g of Ivory Coast cacao beans, 5 kGy of gamma radiation under an atmosphere of air reduced the microflora per g by 2.49 and 3.03 logs at temperatures of 35 and 50 degrees C, respectively. Bahia cacao beans were artificially contaminated with dried spores of A...

journal_title：Applied and environmental microbiology

authors： Restaino L,Myron JJ,Lenovich LM,Bills S,Tscherneff K

更新日期：1984-04-01 00:00:00

abstract：:A mesophilic acetogenic bacterium (MPOB) oxidized propionate to acetate and CO(2) in cocultures with the formate- and hydrogen-utilizing methanogens Methanospirillum hungatei and Methanobacterium formicicum. Propionate oxidation did not occur in cocultures with two Methanobrevibacter strains, which grew only with hydr...

journal_title：Applied and environmental microbiology

authors： Dong X,Plugge CM,Stams AJ

更新日期：1994-08-01 00:00:00

abstract：:A radiometric method for the detection of Salmonella in foods has been developed which is based on Salmonella poly H agglutinating serum preventing Salmonella from producing 14CO2 from [14C]dulcitol. The method will detect the presence or absence of Salmonella in a product within 30 h compared to 4 to 5 days by routin...

journal_title：Applied and environmental microbiology

authors： Stewart BJ,Eyles MJ,Murrell WG

更新日期：1980-08-01 00:00:00

abstract：:Species diversity, phylogenetic affiliations, and environmental occurrence patterns of thiosulfate-oxidizing marine bacteria were investigated by using new isolates from serially diluted continental slope and deep-sea abyssal plain sediments collected off the coast of New England and strains cultured previously from G...

journal_title：Applied and environmental microbiology

authors： Teske A,Brinkhoff T,Muyzer G,Moser DP,Rethmeier J,Jannasch HW

更新日期：2000-08-01 00:00:00

abstract：:A 15-mer peptide fragment derived from pediocin PA-1 (from residue 20 to residue 34) specifically inhibited the bactericidal activity of pediocin PA-1. The fragment did not inhibit the pediocin-like bacteriocins sakacin P, leucocin A, and curvacin A to nearly the same extent as it inhibited pediocin PA-1. Enterocin A,...

journal_title：Applied and environmental microbiology

authors： Fimland G,Jack R,Jung G,Nes IF,Nissen-Meyer J

更新日期：1998-12-01 00:00:00

abstract：:Escherichia coli JM101(pSPZ3), containing xylene monooxygenase (XMO) from Pseudomonas putida mt-2, catalyzes specific oxidations and reductions of m-nitrotoluene and derivatives thereof. In addition to reactions catalyzed by XMO, we focused on biotransformations by native enzymes of the E. coli host and their effect o...

journal_title：Applied and environmental microbiology

authors： Meyer D,Witholt B,Schmid A

更新日期：2005-11-01 00:00:00

abstract：:Estuarine systems are the major conduits for the transfer of nitrate from agricultural and other terrestrial-anthropogenic sources into marine ecosystems. Within estuarine sediments some microbially driven processes (denitrification and anammox) result in the net removal of nitrogen from the environment, while others ...

journal_title：Applied and environmental microbiology

authors： Smith CJ,Nedwell DB,Dong LF,Osborn AM

更新日期：2007-06-01 00:00:00

abstract：:During batch fermentation, the rate of ethanol production per milligram of cell protein is maximal for a brief period early in this process and declines progressively as ethanol accumulates in the surrounding broth. Our studies demonstrate that the removal of this accumulated ethanol does not immediately restore ferme...

journal_title：Applied and environmental microbiology

authors： Dombek KM,Ingram LO

更新日期：1987-06-01 00:00:00

abstract：:Two-dimensional polyacrylamide electrophoresis was used to determine that mutants of Rhizobium trifolii DT6, claimed to be capable of effectively nodulating soybeans, were actually Rhizobium japonicum 110 contaminants isolated from the parent DT6 culture. ...

journal_title：Applied and environmental microbiology

authors： Leps WT,Roberts GP,Brill WJ

更新日期：1980-02-01 00:00:00

abstract：:Continuous centrifugation of large volumes of water from natural southeastern lakes allowed quantitative detection of Legionella pneumophila by direct immunofluorescent staining. Positive samples were injected intraperitoneally into guinea pigs, and the L. pneumophila were isolated and identified by their morphologica...

journal_title：Applied and environmental microbiology

authors： Fliermans CB,Cherry WB,Orrison LH,Thacker L

更新日期：1979-06-01 00:00:00

abstract：:A dot blot hybridization procedure with 32P-labeled whole chromosomal DNA of the type strains as probes was developed as a rapid and simple method for identification of intestinal Bacteroides species. Bacterial cells were fixed onto membrane filters by slight suction, treated with 0.5 N NaOH, and hybridized with these...

journal_title：Applied and environmental microbiology

authors： Morotomi M,Ohno T,Mutai M

更新日期：1988-05-01 00:00:00

abstract：:The bacterial community composition of a linuron-degrading enrichment culture and the role of the individual strains in linuron degradation have been determined by a combination of methods, such as denaturing gradient gel electrophoresis of the total 16S rRNA gene pool, isolation and identification of strains, and bio...

journal_title：Applied and environmental microbiology

authors： Dejonghe W,Berteloot E,Goris J,Boon N,Crul K,Maertens S,Höfte M,De Vos P,Verstraete W,Top EM

更新日期：2003-03-01 00:00:00

abstract：:Enterococci are one of the major facultative anaerobic bacterial groups that reside in the human gastrointestinal tract. In the present study, the composition of the enterococcal fecal flora in three healthy humans was analyzed before, during, and after the daily consumption of approximately 125 g of a raw-milk Chedda...

journal_title：Applied and environmental microbiology

authors： Gelsomino R,Vancanneyt M,Cogan TM,Swings J

更新日期：2003-01-01 00:00:00

abstract：:Ammonia oxidation is a major process in nitrogen cycling, and it plays a key role in nitrogen limited soil ecosystems such as those in the arctic. Although mm-scale spatial dependency of ammonia oxidizers has been investigated, little is known about the field-scale spatial dependency of aerobic ammonia oxidation proce...

journal_title：Applied and environmental microbiology

authors： Banerjee S,Siciliano SD

更新日期：2012-01-01 00:00:00

abstract：:The presence of PCR inhibitors in water samples is well known and contributes to the fact that a practical PCR assay has not been developed for legionella surveillance. In this study, we devised a new seminested PCR assay for detection of Legionella spp. in water samples as a means of overriding the PCR inhibitors wit...

journal_title：Applied and environmental microbiology

authors： Miyamoto H,Yamamoto H,Arima K,Fujii J,Maruta K,Izu K,Shiomori T,Yoshida S

更新日期：1997-07-01 00:00:00

abstract：:Gibberella moniliformis (Fusarium verticillioides) is primarily a pathogen of maize, but it can also cause disease in other crop species. This pathogenicity, as well as the contamination of food- and feedstuffs with the fumonisin mycotoxins, results in economically significant losses to both farmers and food processor...

journal_title：Applied and environmental microbiology

authors： Jurgenson JE,Zeller KA,Leslie JF

更新日期：2002-04-01 00:00:00

abstract：:Cyanobacterial hepatotoxins, microcystins, are specific inhibitors of serine/threonine protein phosphatases and potent tumor promoters. They have caused several poisonings of animals and also pose a health hazard for humans through the use of water for drinking and recreation. Different strains of the same cyanobacter...

journal_title：Applied and environmental microbiology

authors： Rapala J,Sivonen K,Lyra C,Niemelä SI

更新日期：1997-06-01 00:00:00

abstract：UNLABELLED:Raw sausages are perishable foodstuffs; reducing their salt content raises questions about a possible increased spoilage of these products. In this study, we evaluated the influence of salt reduction (from 2.0% to 1.5% [wt/wt]), in combination with two types of packaging (modified atmosphere [50% mix of CO2-...

journal_title：Applied and environmental microbiology

authors： Fougy L,Desmonts MH,Coeuret G,Fassel C,Hamon E,Hézard B,Champomier-Vergès MC,Chaillou S

更新日期：2016-06-13 00:00:00

abstract：:Environmental studies of the human-pathogenic bacterium Campylobacter jejuni have focused on linking distributions with potential sources. However, in aquatic ecosystems, the abundance of C. jejuni may also be regulated by predation. We examine the potential for grazing by the freshwater planktonic crustacean Daphnia ...

journal_title：Applied and environmental microbiology

authors： Schallenberg M,Bremer PJ,Henkel S,Launhardt A,Burns CW

更新日期：2005-09-01 00:00:00

abstract：:The human gut is host to a diversity of microorganisms, including the single-celled microbial eukaryote Blastocystis. Research has shown that most carriers host a single Blastocystis subtype (ST), which is unusual given the considerable within-host species diversity observed for other microbial genera in this ecosyste...

journal_title：Applied and environmental microbiology

authors： Scanlan PD,Stensvold CR,Cotter PD

更新日期：2015-06-15 00:00:00

abstract：:Stachybotrys atra is the etiologic agent of stachybotryotoxicosis, and this fungus and its trichothecene mycotoxins were recently implicated in an outbreak of unexplained illness in homes. S. atra was grown on sterile rice, autoclaved, dried, and then aerosolized by acoustic vibration. The distribution of particles (m...

journal_title：Applied and environmental microbiology

authors： Sorenson WG,Frazer DG,Jarvis BB,Simpson J,Robinson VA

更新日期：1987-06-01 00:00:00