Suitability of recombinant Escherichia coli and Pseudomonas putida strains for selective biotransformation of m-nitrotoluene by xylene monooxygenase.

abstract：:[This corrects the article on p. 514 in vol. 55.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1989-06-01 00:00:00

abstract：:Isolation and cultivation are a crucial step in elucidating the physiology, biogeochemistry, and ecosystem role of microorganisms. Many abundant marine bacteria, including the widespread Roseobacter clade-affiliated (RCA) cluster group, have not been cultured with traditional methods. Using novel techniques of coculti...

journal_title：Applied and environmental microbiology

authors： Mayali X,Franks PJ,Azam F

更新日期：2008-05-01 00:00:00

abstract：:An indirect fluorescent antibody-membrane filter staining technique, which permitted the autecological study of Methylomonas methanica and Methylosinus trichosporium, was developed. This technique was used to assay the numbers of these two organisms in Cleveland Harbor. The concentrations of M. methanica and M. tricho...

journal_title：Applied and environmental microbiology

authors： Reed WM,Dugan PR

更新日期：1978-02-01 00:00:00

abstract：:Fatty acid compositions in growing and resting cells of several strains of Pseudomonas putida (P8, NCTC 10936, and KT 2440) were studied, with a focus on alterations of the saturation degree, cis-trans isomerization, and cyclopropane formation. The fatty acid compositions of the strains were very similar under compara...

journal_title：Applied and environmental microbiology

authors： Härtig C,Loffhagen N,Harms H

更新日期：2005-04-01 00:00:00

abstract：:The prokaryote community activity and structural characteristics within marine sediment sampled across a continental shelf area located off eastern Antarctica (66 degrees S, 143 degrees E; depth range, 709 to 964 m) were studied. Correlations were found between microbial biomass and aminopeptidase and chitinase rates,...

journal_title：Applied and environmental microbiology

authors： Bowman JP,McCammon SA,Gibson JA,Robertson L,Nichols PD

更新日期：2003-05-01 00:00:00

abstract：:Three different gels (Sepharose 4B, Sephadex G-200, and Sephadex G-50) were evaluated as a means of removing humic contaminants from DNA extracts of environmental samples. Sepharose 4B gave superior separation of DNA from humics, and DNA purified in this way showed consistently greater amplification than DNA purified ...

journal_title：Applied and environmental microbiology

authors： Jackson CR,Harper JP,Willoughby D,Roden EE,Churchill PF

更新日期：1997-12-01 00:00:00

abstract：:Acropora and Porites corals are important reef builders in the Indo-Pacific and Caribbean. Bacteria associated with mucus produced by Porites spp. and Acropora spp. from Caribbean (Punta Maroma, Mexico) and Indo-Pacific (Hoga and Sampela, Indonesia) reefs were determined. Analysis of pyrosequencing libraries showed th...

journal_title：Applied and environmental microbiology

authors： McKew BA,Dumbrell AJ,Daud SD,Hepburn L,Thorpe E,Mogensen L,Whitby C

更新日期：2012-08-01 00:00:00

abstract：:Higher yields of sterigmatocystin were obtained with Aspergillus versicolor than with Bipolaris sorokiniana both in liquid and on solid media. The optimum temperature for sterigmatocystin production by A. versicolor was 27 to 29 degrees C and 23 degrees C for B. sorokiniana. In liquid shake cultures, production of ste...

journal_title：Applied and environmental microbiology

authors： Rabie CJ,Lubben A,Steyn M

更新日期：1976-08-01 00:00:00

abstract：:Pseudomonas aeruginosa and Vibrio cholerae showed a strong positive chemotactic response towards rum distillery wastewaters (mostos) and a high oxygen uptake rate in the presence of this complex substrate. Rum slops stimulated only motility in Aeromonas hydrophila and Escherichia coli. The A. hydrophila and E. coli is...

journal_title：Applied and environmental microbiology

authors： Fuentes FA,Biamon EJ,Hazen TC

更新日期：1983-12-01 00:00:00

abstract：:Quantitative assessment of growth of filamentous microorganisms, such as streptomycetes, is generally restricted to determination of dry weight. Here, we describe a straightforward methylene blue-based sorption assay to monitor microbial growth quantitatively, simply, and rapidly. The assay is equally applicable to un...

journal_title：Applied and environmental microbiology

authors： Fischer M,Sawers RG

更新日期：2013-07-01 00:00:00

abstract：:The luminescence signal of luxAB-encoded bacterial luciferase strongly depends on the metabolic state of the host cell, which restricts the use of this reporter system to metabolically active bacteria. Here we show that in stationary-phase cells of Lactococcus lactis, detection of luciferase is significantly improved ...

journal_title：Applied and environmental microbiology

authors： Bachmann H,Santos F,Kleerebezem M,van Hylckama Vlieg JE

更新日期：2007-07-01 00:00:00

abstract：:Escherichia coli strains harboring deletion mutations of the insecticidal protoxin gene of Bacillus thuringiensis subsp. berliner 1715 were constructed. Although these strains did not produce intact protoxin, cell extracts from one of the mutants were extremely toxic to tobacco hornworm (Manduca sexta) larvae, indicat...

journal_title：Applied and environmental microbiology

authors： Wabiko H,Held GA,Bulla LA Jr

更新日期：1985-03-01 00:00:00

abstract：:Gram-positive bacteria of the genus Exiguobacterium have been repeatedly isolated from Siberian permafrost ranging in age from 20,000 to 2 to 3 million years and have been sporadically recovered from markedly diverse habitats, including microbial mats in Lake Fryxell (Antarctic), surface water, and food-processing env...

journal_title：Applied and environmental microbiology

authors： Vishnivetskaya TA,Kathariou S

更新日期：2005-11-01 00:00:00

abstract：:From Bradyrhizobium japonicum highly reiterated sequence-possessing (HRS) strains indigenous to Niigata and Tokachi in Japan with high copy numbers of the repeated sequences RSalpha and RSbeta (K. Minamisawa, T. Isawa, Y. Nakatsuka, and N. Ichikawa, Appl. Environ. Microbiol. 64:1845-1851, 1998), several insertion sequ...

journal_title：Applied and environmental microbiology

authors： Isawa T,Sameshima R,Mitsui H,Minamisawa K

更新日期：1999-08-01 00:00:00

abstract：:A novel flagellatropic phage of Salmonella enterica serovar Typhimurium, called iEPS5, was isolated and characterized. iEPS5 has an icosahedral head and a long noncontractile tail with a tail fiber. Genome sequencing revealed a double-stranded DNA of 59,254 bp having 73 open reading frames (ORFs). To identify the rece...

journal_title：Applied and environmental microbiology

authors： Choi Y,Shin H,Lee JH,Ryu S

更新日期：2013-08-01 00:00:00

abstract：:The ability of indigenous Rhizobium leguminosarum and Rhizobium meliloti to use organic nutrients as growth substrates in soil was assessed by indirect bacteriophage analysis. A total of 17 organic compounds, including 9 carbohydrates, 3 organic acids, and 5 amino acids, were tested (1,000 mug g) in three soils with d...

journal_title：Applied and environmental microbiology

authors： Germida JJ

更新日期：1988-01-01 00:00:00

abstract：:The influence of substrate and enzyme concentrations on the rate of saccharification of two defined insoluble cellulose substrates, Avicel (FMC Corp., Philadelphia, Pa.) and Solka-Floc (James River Co., Berlin, N.H.), by the cellulase enzyme system of Trichoderma viride was evaluated. In the assays, enzyme concentrati...

journal_title：Applied and environmental microbiology

authors： Liaw ET,Penner MH

更新日期：1990-08-01 00:00:00

abstract：:The industrial production of beta-carotene with the zygomycete Blakeslea trispora involves the joint cultivation of mycelia of opposite sex in the presence of beta-ionone and other chemical activators. We have obtained improved strains by mutation and heterokaryosis. We chose wild strains on the basis of their growth ...

journal_title：Applied and environmental microbiology

authors： Mehta BJ,Obraztsova IN,Cerdá-Olmedo E

更新日期：2003-07-01 00:00:00

abstract：:Sequencing of plasmid pLM33 from the food isolate Listeria monocytogenes Lm1 revealed a molecule of 32,307 bp with a G+C content of 36.2%. The plasmid displays a mosaic pattern of identities common to several closely related L. monocytogenes plasmids isolated from food and clinical sources. ...

journal_title：Applied and environmental microbiology

authors： Canchaya C,Giubellini V,Ventura M,de los Reyes-Gavilán CG,Margolles A

更新日期：2010-07-01 00:00:00

abstract：:The als gene for alpha-acetolactate synthase of Lactococcus lactis MG1363 was cloned on a multicopy plasmid under the control of the inducible L. lactis lacA promoter. More than a hundredfold overproduction of alpha-acetolactate synthase was obtained in L. lactis under inducing conditions as compared with that of the ...

journal_title：Applied and environmental microbiology

authors： Platteeuw C,Hugenholtz J,Starrenburg M,van Alen-Boerrigter I,de Vos WM

更新日期：1995-11-01 00:00:00

abstract：:The beta-xylosidase-encoding xlnD gene of Aspergillus niger 90196 was amplified by the PCR technique from first-strand cDNA synthesized on mRNA isolated from the fungus. The nucleotide sequence of the cDNA fragment was verified to contain a 2,412-bp open reading frame that encodes a 804-amino-acid propeptide. The 778-...

journal_title：Applied and environmental microbiology

authors： La Grange DC,Pretorius IS,Claeyssens M,van Zyl WH

更新日期：2001-12-01 00:00:00

abstract：:Equations defining the reaction of microbial alginase on commercial sodium alginate are presented with respect to the viscometric assay of enzyme activity. The negative log of K was found to be linearly related to the concentration of algin, where K is defined as the change in the reciprocal of relative viscosity with...

journal_title：Applied and environmental microbiology

authors： Stevens RA,Levin RE

更新日期：1976-06-01 00:00:00

abstract：:Bacteria able to perform dissimilatory nitrate reduction to ammonium were isolated from low-oxygen masses in the Baltic Sea. In liquid media enriched with NO(3) and incubated anaerobically, the NH(4)-producing isolates transformed 25 to 72% of the NO(3) to NH(4). ...

journal_title：Applied and environmental microbiology

authors： Samuelsson MO,Rönner U

更新日期：1982-11-01 00:00:00

abstract：:Bacteriophages are the source of many valuable tools for molecular biology and genetic manipulation. In Streptomyces, most DNA cloning vectors are based on serine integrase site-specific DNA recombination systems derived from phage. Because of their efficiency and simplicity, serine integrases are also used for divers...

journal_title：Applied and environmental microbiology

authors： Fogg PCM,Haley JA,Stark WM,Smith MCM

更新日期：2017-02-15 00:00:00

abstract：:Six monoclonal antibodies directed against enterobacteria were produced and characterized. The specificity of one of these antibodies (CX9/15; immunoglobulin G2a) was studied by indirect immunofluorescence against 259 enterobacterial strains and 125 other gram-negative bacteria. All of the enterobacteria were specific...

journal_title：Applied and environmental microbiology

authors： Levasseur S,Husson MO,Leitz R,Merlin F,Laurent F,Peladan F,Drocourt JL,Leclerc H,Van Hoegaerden M

更新日期：1992-05-01 00:00:00

abstract：:Diversity among 130 strains of Bacillus polymyxa was studied; the bacteria were isolated by immunotrapping from nonrhizosphere soil (32 strains), rhizosphere soil (38 strains), and the rhizoplane (60 strains) of wheat plantlets growing in a growth chamber. The strains were characterized phenotypically by 63 auxanograp...

journal_title：Applied and environmental microbiology

authors： Mavingui P,Laguerre G,Berge O,Heulin T

更新日期：1992-06-01 00:00:00

abstract：:A simple gel immunodiffusion agar procedure was developed for detecting toxigenic strains of Clostridium botulinum type A. The method consisted of overlaying colonies grown on thin-layer tryptone-peptone-glucose-yeast extract agar with gel diffusion agar containing desired levels of C. botulinum type A antitoxin. Conc...

journal_title：Applied and environmental microbiology

authors： Ferreira JL,Hamdy MK,Zapatka FA,Hebert WO

更新日期：1981-12-01 00:00:00

abstract：:Quantitation and detection of xylem-limited bacteria with an enzyme-linked immunosorbent assay with a peroxidase conjugate is described. The use of the Trinder reagent (4-aminoantipyrine) allows the determination of extremely small quantities of peroxidase with no precipitate formation or inactivation of the enzyme by...

journal_title：Applied and environmental microbiology

authors： Saunders MS,French WJ

更新日期：1983-08-01 00:00:00

abstract：:Pot experiments showed that Rhizobium japonicum cells entrapped in a polyacrylamide gel could be used as an inoculant for soybeans and compared favorably to laboratory-made peat base inoculant containing the same bacterial strain. ...

journal_title：Applied and environmental microbiology

authors： Dommergues YR,Diem HG,Divies C

更新日期：1979-04-01 00:00:00

abstract：:We examined the genetic structure and symbiotic characteristics of Bradyrhizobium isolates recovered from four legume species (Lupinus albus [white lupine], Lupinus angustifolius [blue lupine], Ornithopus compressus [yellow serradella], and Macroptilium atropurpureum [sirato]) grown in an Oregon soil. We established t...

journal_title：Applied and environmental microbiology

authors： Bottomley PJ,Cheng HH,Strain SR

更新日期：1994-06-01 00:00:00