Abstract:
:Six monoclonal antibodies directed against enterobacteria were produced and characterized. The specificity of one of these antibodies (CX9/15; immunoglobulin G2a) was studied by indirect immunofluorescence against 259 enterobacterial strains and 125 other gram-negative bacteria. All of the enterobacteria were specifically recognized, the only exception being Erwinia chrysanthemi (one strain tested). Bacteria not belonging to members of the family Enterobacteriaceae were not detected, except for Plesiomonas shigelloides (two strains tested), Aeromonas hydrophila (five strains tested), and Aeromonas sobria (one strain tested). This recognition spectrum strongly suggested that CX9/15 recognized the enterobacterial common antigen. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot (immunoblot) experiments, the six antienterobacteria antibodies presented similar specificities; they all revealed only one band with an apparent molecular weight of about 20,000 from the crude extract of an enterobacterium. The six monoclonal antibodies, and especially CX9/15, can be used to develop new tests for rapid and specific detection of enterobacteria.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Levasseur S,Husson MO,Leitz R,Merlin F,Laurent F,Peladan F,Drocourt JL,Leclerc H,Van Hoegaerden Mdoi
10.1128/AEM.58.5.1524-1529.1992keywords:
subject
Has Abstractpub_date
1992-05-01 00:00:00pages
1524-9issue
5eissn
0099-2240issn
1098-5336journal_volume
58pub_type
杂志文章abstract::Hybridization of crushed Lotus pedunculatus root nodules with P-labeled total genomic DNA probes was used to identify Rhizobium loti and Bradyrhizobium sp. (Lotus rhizobia). Probes always hybridized with homologous target DNA and frequently with DNAs of other strains from the same genus. Intergeneric hybridization did...
journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.53.7.1705-1707.1987
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doi:10.1128/AEM.00596-11
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doi:10.1128/AEM.60.6.2152-2154.1994
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.58.4.1364-1366.1992
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.1.180-186.1995
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pub_type: 杂志文章
doi:10.1128/AEM.65.12.5576-5585.1999
更新日期:1999-12-01 00:00:00
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.59.4.960-967.1993
更新日期:1993-04-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.11.3744-3749.1993
更新日期:1993-11-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.42.6.1037-1042.1981
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.70.1.625-630.2004
更新日期:2004-01-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2009-07-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01505-18
更新日期:2018-10-17 00:00:00