Molecular characterization of a toluene-degrading methanogenic consortium.

Abstract:

:A toluene-degrading methanogenic consortium enriched from creosote-contaminated aquifer material was maintained on toluene as the sole carbon and energy source for 10 years. The species in the consortium were characterized by using a molecular approach. Total genomic DNA was isolated, and 16S rRNA genes were amplified by using PCR performed with kingdom-specific primers that were specific for 16S rRNA genes from either members of the kingdom Bacteria or members of the kingdom Archaea. A total of 90 eubacterial clones and 75 archaeal clones were grouped by performing a restriction fragment length polymorphism (RFLP) analysis. Six eubacterial sequences and two archaeal sequences were found in the greatest abundance (in six or more clones) based on the RFLP analysis. The relative abundance of each putative species was estimated by using fluorescent in situ hybridization (FISH), and the presence of putative species was determined qualitatively by performing slot blot hybridization with consortium DNA. Both archaeal species and two of the six eubacterial species were detected in the DNA and FISH hybridization experiments. A phylogenetic analysis of these four dominant organisms suggested that the two archaeal species are related to the genera Methanosaeta and Methanospirillum. One of the eubacterial species is related to the genus Desulfotomaculum, while the other is not related to any previously described genus. By elimination, we propose that the last organism probably initiates the attack on toluene.

journal_name

Appl Environ Microbiol

authors

Ficker M,Krastel K,Orlicky S,Edwards E

doi

10.1128/AEM.65.12.5576-5585.1999

keywords:

subject

Has Abstract

pub_date

1999-12-01 00:00:00

pages

5576-85

issue

12

eissn

0099-2240

issn

1098-5336

journal_volume

65

pub_type

杂志文章
  • Species-specific cell wall binding affinity of the S-layer proteins of mosquitocidal bacterium Bacillus sphaericus C3-41.

    abstract::The binding affinities and specificities of six truncated S-layer homology domain (SLH) polypeptides of mosquitocidal Bacillus sphaericus strain C3-41 with the purified cell wall sacculi have been assayed. The results indicated that the SLH polypeptide comprised of amino acids 31 to 210 was responsible for anchoring t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00356-09

    authors: Li J,Hu X,Yan J,Yuan Z

    更新日期:2009-06-01 00:00:00

  • Small pectinolytic spirochetes from the rumen.

    abstract::Two strains of small spirochetes utilizing pectin as sole source of energy for growth were isolated from the rumen of cattle and partially characterized. The pectinolytic enzyme produced by one of the organisms was classified as a poly-1,4-alpha-D-galacturonide lyase (EC 4.2.2.2). A pectinesterase (EC 3.1.1.11) was al...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.39.4.919-922.1980

    authors: Ziołecki A,Wojciechowicz M

    更新日期:1980-04-01 00:00:00

  • Diversity of Salmonella strains isolated from the aquatic environment as determined by serotyping and amplification of the ribosomal DNA spacer regions.

    abstract::Salmonella species are pathogenic bacteria often detected in sewage, freshwater, marine coastal water, and groundwater. Salmonella spp. can survive for long periods in natural waters, and the persistence of specific and epidemic strains is of great concern in public health. However, the diversity of species found in t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.4.1544-1552.2000

    authors: Baudart J,Lemarchand K,Brisabois A,Lebaron P

    更新日期:2000-04-01 00:00:00

  • An Intramolecular Salt Bridge in Bacillus thuringiensis Cry4Ba Toxin Is Involved in the Stability of Helix α-3, Which Is Needed for Oligomerization and Insecticidal Activity.

    abstract::Bacillus thuringiensis three-domain Cry toxins kill insects by forming pores in the apical membrane of larval midgut cells. Oligomerization of the toxin is an important step for pore formation. Domain I helix α-3 participates in toxin oligomerization. Here we identify an intramolecular salt bridge within helix α-3 of ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01515-17

    authors: Pacheco S,Gómez I,Sánchez J,García-Gómez BI,Soberón M,Bravo A

    更新日期:2017-09-29 00:00:00

  • Evaluation of molecular typing techniques to assign genetic diversity among Saccharomyces cerevisiae strains.

    abstract::Discrimination of strains within the species Saccharomyces cerevisiae was demonstrated by the use of four different techniques to type 15 strains isolated from spoiled wine and beer. Random amplified polymorphic DNA with specific oligonucleotides and PCR fingerprinting with the microsatellite oligonucleotide primers (...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.1.41-46.1996

    authors: Baleiras Couto MM,Eijsma B,Hofstra H,Huis in't Veld JH,van der Vossen JM

    更新日期:1996-01-01 00:00:00

  • In Situ Hydrogen Dynamics in a Hot Spring Microbial Mat during a Diel Cycle.

    abstract:UNLABELLED:Microbes can produce molecular hydrogen (H2) via fermentation, dinitrogen fixation, or direct photolysis, yet the H2 dynamics in cyanobacterial communities has only been explored in a few natural systems and mostly in the laboratory. In this study, we investigated the diel in situ H2 dynamics in a hot spring...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00710-16

    authors: Revsbech NP,Trampe E,Lichtenberg M,Ward DM,Kühl M

    更新日期:2016-06-30 00:00:00

  • High rates of sulfate reduction in a low-sulfate hot spring microbial mat are driven by a low level of diversity of sulfate-respiring microorganisms.

    abstract::The importance of sulfate respiration in the microbial mat found in the low-sulfate thermal outflow of Mushroom Spring in Yellowstone National Park was evaluated using a combination of molecular, microelectrode, and radiotracer studies. Despite very low sulfate concentrations, this mat community was shown to sustain a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00357-07

    authors: Dillon JG,Fishbain S,Miller SR,Bebout BM,Habicht KS,Webb SM,Stahl DA

    更新日期:2007-08-01 00:00:00

  • Stable transformation of the Xylella fastidiosa citrus variegated chlorosis strain with oriC plasmids.

    abstract::Xylella fastidiosa is a gram-negative, xylem-limited bacterium affecting economically important crops (e.g., grapevine, citrus, and coffee). The citrus variegated chlorosis (CVC) strain of X. fastidiosa is the causal agent of this severe disease of citrus in Brazil and represents the first plant-pathogenic bacterium f...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.5.2263-2269.2001

    authors: Monteiro PB,Teixeira DC,Palma RR,Garnier M,Bové JM,Renaudin J

    更新日期:2001-05-01 00:00:00

  • Production and properties of cyanobacterial endotoxins.

    abstract::Lipopolysaccharides (LPS) were isolated from four species of cyanobacteria (Anabaena flos-aquae UTEX 1444. A. cylindrica, Oscillatoria tenuis, and O. brevis) frequently occurring in drinking-water supplies. The cyanobacterial LPS contained glucose, xylose, mannose, and rhamnose, but differed from the LPS derived from ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.43.1.104-109.1982

    authors: Keleti G,Sykora JL

    更新日期:1982-01-01 00:00:00

  • Thermophilic methanogenesis in a hot-spring algal-bacterial mat (71 to 30 degrees C).

    abstract::Algal-bacterial mats which grow in the effluent channels of alkaline hot springs provided an environment suitable for studying natural thermophilic methane producing bacteria. Methane was rapidly produced in cores taken from the meat and appeared to be an end product of decomposition of the algal-bacterial organic mat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.35.6.1019-1026.1978

    authors: Ward DM

    更新日期:1978-06-01 00:00:00

  • Persistence and growth of fecal Bacteroidales assessed by bromodeoxyuridine immunocapture.

    abstract::Extraintestinal growth of fecal bacteria can impair accurate assessment of watershed health. Anaerobic fecal bacteria belonging to the order Bacteroidales are attractive candidates for fecal source tracking because they have host-specific distributions and do not grow well in the presence of high oxygen concentrations...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00038-06

    authors: Walters SP,Field KG

    更新日期:2006-07-01 00:00:00

  • Viability of Rhizobium bacteroids.

    abstract::Bacteroids prepared from nodules of soybean and bean were tested for viability. Contrary to the prevailing view that bacteroids are nonviable, it was found that bacteroids averaged 90% viability, irrespective of Rhizobium strain, nodule age, or nodule environment. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.34.6.854-856.1977

    authors: Tsien HC,Cain PS,Schmidt EL

    更新日期:1977-12-01 00:00:00

  • Divergent Nrf Family Proteins and MtrCAB Homologs Facilitate Extracellular Electron Transfer in Aeromonas hydrophila.

    abstract::Extracellular electron transfer (EET) is a strategy for respiration in which electrons generated from metabolism are moved outside the cell to a terminal electron acceptor, such as iron or manganese oxide. EET has primarily been studied in two model systems, Shewanella oneidensis and Geobacter sulfurreducens Metal red...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02134-18

    authors: Conley BE,Intile PJ,Bond DR,Gralnick JA

    更新日期:2018-11-15 00:00:00

  • Derivation of DNA probes for enumeration of a specific strain of Lactobacillus acidophilus in piglet digestive tract samples.

    abstract::Four DNA probes were derived that hybridized specifically to DNA from Lactobacillus acidophilus O. The probes were constructed by randomly cloning lactobacillus DNA in plasmid vector pBR322. Two of the probes (pSR1 and pSR2) were composed of vector and plasmid DNA inserts (3.6 and 1.6 kb, respectively); the others (pS...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.11.3871-3877.1993

    authors: Rodtong S,Dobbinson S,Thode-Andersen S,McConnell MA,Tannock GW

    更新日期:1993-11-01 00:00:00

  • Characterization of a new pathway for epichlorohydrin degradation by whole cells of xanthobacter strain py2.

    abstract::The degradation of epichlorohydrin (3-chloropropylene oxide or 1-chloro-2,3-epoxypropane) by whole-cell suspensions of Xanthobacter strain Py2 was investigated. Cell suspensions prepared from cultures grown with propylene as the carbon source readily degraded epichlorohydrin. The ability to degrade epichlorohydrin cor...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.4.1507-1513.1995

    authors: Small FJ,Tilley JK,Ensign SA

    更新日期:1995-04-01 00:00:00

  • Rapid purification of the oxygenase component of toluene dioxygenase from a polyol-responsive monoclonal antibody.

    abstract::A monoclonal antibody designated 302 beta that is specific for the beta subunit of the oxygenase component (ISPTOL) of toluene dioxygenase from Pseudomonas putida F1 was used to prepare an immunoaffinity column. ISPTOL in cell extracts of Escherichia coli JM109(pDTG611) bound to the column, and an enzyme-linked immuno...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.6.2133-2137.1996

    authors: Lynch NA,Jiang H,Gibson DT

    更新日期:1996-06-01 00:00:00

  • Selective phylogenetic analysis targeted at 16S rRNA genes of thermophiles and hyperthermophiles in deep-subsurface geothermal environments.

    abstract::Deep-subsurface samples obtained by deep drilling are likely to be contaminated with mesophilic microorganisms in the drilling fluid, and this could affect determination of the community structure of the geothermal microflora using 16S rRNA gene clone library analysis. To eliminate possible contamination by PCR-amplif...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.72.1.21-27.2006

    authors: Kimura H,Sugihara M,Kato K,Hanada S

    更新日期:2006-01-01 00:00:00

  • Aflatoxin contamination in soybeans: role of proteinase inhibitors, zinc availability, and seed coat integrity.

    abstract::Soybean trypsin inhibitors are thought to ward off pathogens. Studies with aflatoxigenic strains of Aspergillus flavus and A. parasiticus, frequent soybean contaminants, revealed that trypsin inhibitors do not affect the growth of these fungi and aflatoxin production. Further, the availability of zinc, an essential mi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.52.1.68-72.1986

    authors: Stössel P

    更新日期:1986-07-01 00:00:00

  • Cloning and expression of a novel toxin gene from Bacillus thuringiensis subsp. jegathesan encoding a highly mosquitocidal protein.

    abstract::A gene, designated cry11B, encoding a 81,293-Da crystal protein of Bacillus thuringiensis subsp. jegathesan was cloned by using a gene-specific oligonucleotide probe. The sequence of the Cry11B protein, as deduced from the sequence of the cry11B gene, contains large regions of similarity with the Cry11A toxin (previou...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.12.4230-4235.1995

    authors: Delécluse A,Rosso ML,Ragni A

    更新日期:1995-12-01 00:00:00

  • Types and distribution of anaerobic bacteria in the large intestine of pigs.

    abstract::An examination was made of various sites along the length of the swine large intestine, using strictly anaerobic culture methods. Sites were separated by differential washing into fractions described as lumenal content, lumenal surface layer, and intestinal wall tissue. Direct microscopic clump counts averaged 13.3 x ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.37.2.187-193.1979

    authors: Russell EG

    更新日期:1979-02-01 00:00:00

  • Bacteriophages active against Bacteroides fragilis in sewage-polluted waters.

    abstract::Twelve strains of different Bacteroides species were tested for their efficiency of detection of bacteriophages from sewage. The host range of several isolated phages was investigated. The results indicated that there was a high degree of strain specificity. Then, by using Bacteroides fragilis HSP 40 as the host, whic...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.7.1632-1637.1987

    authors: Tartera C,Jofre J

    更新日期:1987-07-01 00:00:00

  • Manganese Peroxidase, Produced by Trametes versicolor during Pulp Bleaching, Demethylates and Delignifies Kraft Pulp.

    abstract::Previous work has shown that Trametes (Coriolus) versicolor bleaches kraft pulp brownstock with the concomitant release of methanol. In this work, the fungus is shown to produce both laccase and manganese peroxidase (MnP) but not lignin peroxidase during pulp bleaching. MnP production was enhanced by the presence of p...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.1.260-265.1993

    authors: Paice MG,Reid ID,Bourbonnais R,Archibald FS,Jurasek L

    更新日期:1993-01-01 00:00:00

  • Use of the polymerase chain reaction in detection of culturable and nonculturable Vibrio vulnificus cells.

    abstract::Vibrio vulnificus is a human pathogen associated with consumption of raw oysters. During the colder months the organism apparently enters a viable but nonculturable state and thus cannot be cultured by ordinary bacteriological methods. For this reason, another means of detecting this bacterium is necessary. In the pre...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.9.2651-2655.1991

    authors: Brauns LA,Hudson MC,Oliver JD

    更新日期:1991-09-01 00:00:00

  • Production and Biotechnological Potential of Extracellular Polymeric Substances from Sponge-Associated Antarctic Bacteria.

    abstract::Four sponge-associated Antarctic bacteria (i.e., Winogradskyella sp. strains CAL384 and CAL396, Colwellia sp. strain GW185, and Shewanella sp. strain CAL606) were selected for the highly mucous appearance of their colonies on agar plates. The production of extracellular polymeric substances (EPSs) was enhanced by a st...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01624-17

    authors: Caruso C,Rizzo C,Mangano S,Poli A,Di Donato P,Finore I,Nicolaus B,Di Marco G,Michaud L,Lo Giudice A

    更新日期:2018-01-31 00:00:00

  • Evaluation of F-specific RNA bacteriophage as a candidate human enteric virus indicator for bivalve molluscan shellfish.

    abstract::Escherichia coli is a widely utilized indicator of the sanitary quality of bivalve molluscan shellfish sold for human consumption. However, it is now well documented that shellfish that meet the E. coli standards for human consumption may contain human enteric viruses that cause gastroenteritis and hepatitis. In this ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.4.1280-1285.2000

    authors: Doré WJ,Henshilwood K,Lees DN

    更新日期:2000-04-01 00:00:00

  • Different IS629 transposition frequencies exhibited by Escherichia coli O157:H7 strains in the stepwise evolutionary model.

    abstract::The insertion sequence IS629, which is highly prevalent in Escherichia coli O157:H7 genomes, was found to be absent in O157:H- strains, which are on a divergent pathway in the emergence of O157:H7. Although O157:H- is deficient in IS629, it permits IS629 transposition, with an excision frequency higher than that of an...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00249-11

    authors: Rump LV,Fischer M,González-Escalona N

    更新日期:2011-07-01 00:00:00

  • Successful predation of filamentous bacteria by a nanoflagellate challenges current models of flagellate bacterivory.

    abstract::Current models suggest that (i) filamentous bacteria are protected against predation by nanoflagellates, (ii) prey size is positively correlated with prey-predator contact probability, and (iii) contact probability is mainly responsible for size-selective predation by interception-feeding flagellates. We used five str...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.70.1.332-339.2004

    authors: Wu QL,Boenigk J,Hahn MW

    更新日期:2004-01-01 00:00:00

  • Identification by PCR of Fusarium culmorum strains producing large and small amounts of deoxynivalenol.

    abstract::Thirty deoxynivalenol-producing F. culmorum strains, isolated from wheat grains, were incubated in vitro and analyzed for trichothecene production. Seventeen strains produced more than 1 ppm of deoxynivalenol and acetyldeoxynivalenol and were considered high-deoxynivalenol-producing strains, whereas 13 F. culmorum str...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.11.5472-5479.2002

    authors: Bakan B,Giraud-Delville C,Pinson L,Richard-Molard D,Fournier E,Brygoo Y

    更新日期:2002-11-01 00:00:00

  • Phenolic Amides Are Potent Inhibitors of De Novo Nucleotide Biosynthesis.

    abstract::An outstanding challenge toward efficient production of biofuels and value-added chemicals from plant biomass is the impact that lignocellulose-derived inhibitors have on microbial fermentations. Elucidating the mechanisms that underlie their toxicity is critical for developing strategies to overcome them. Here, using...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01324-15

    authors: Pisithkul T,Jacobson TB,O'Brien TJ,Stevenson DM,Amador-Noguez D

    更新日期:2015-09-01 00:00:00

  • The Phylloplane as a Source of Bacillus thuringiensis Variants.

    abstract::Novel variants of Bacillus thuringiensis were isolated from the phylloplane of deciduous and conifer trees as well as of other plants. These isolates displayed a range of toxicity towards Trichoplusia ni. Immunoblot and toxin protein analysis indicate that these strains included representatives of the three principal ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.1.311-315.1991

    authors: Smith RA,Couche GA

    更新日期:1991-01-01 00:00:00