Identification by PCR of Fusarium culmorum strains producing large and small amounts of deoxynivalenol.

Abstract:

:Thirty deoxynivalenol-producing F. culmorum strains, isolated from wheat grains, were incubated in vitro and analyzed for trichothecene production. Seventeen strains produced more than 1 ppm of deoxynivalenol and acetyldeoxynivalenol and were considered high-deoxynivalenol-producing strains, whereas 13 F. culmorum strains produced less than 0.07 ppm of trichothecenes and were considered low-deoxynivalenol-producing strains. For all strains, a 550-base portion of the trichodiene synthase gene (tri5) was amplified and sequenced. According to the tri5 data, the F. culmorum strains tested clustered into two groups that correlated with in vitro deoxynivalenol production. For three high-producing and three low-producing F. culmorum strains, the tri5-tri6 intergenic region was then sequenced, which confirmed the two separate clusters within the F. culmorum strains. According to the tri5-tri6 sequence data, specific PCR primers were designed to allow differentiation of high-producing from low-producing F. culmorum strains.

journal_name

Appl Environ Microbiol

authors

Bakan B,Giraud-Delville C,Pinson L,Richard-Molard D,Fournier E,Brygoo Y

doi

10.1128/aem.68.11.5472-5479.2002

keywords:

subject

Has Abstract

pub_date

2002-11-01 00:00:00

pages

5472-9

issue

11

eissn

0099-2240

issn

1098-5336

journal_volume

68

pub_type

杂志文章
  • Melanin Produced by the Fast-Growing Marine Bacterium Vibrio natriegens through Heterologous Biosynthesis: Characterization and Application.

    abstract::Melanin is a pigment produced by organisms throughout all domains of life. Due to its unique physicochemical properties, biocompatibility, and biostability, there has been an increasing interest in the use of melanin for broad applications. In the vast majority of studies, melanin has been either chemically synthesize...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02749-19

    authors: Wang Z,Tschirhart T,Schultzhaus Z,Kelly EE,Chen A,Oh E,Nag O,Glaser ER,Kim E,Lloyd PF,Charles PT,Li W,Leary D,Compton J,Phillips DA,Dhinojwala A,Payne GF,Vora GJ

    更新日期:2020-02-18 00:00:00

  • Analysis of Campylobacter jejuni Subtype Distribution in the Chicken Broiler Production Continuum: a Longitudinal Examination To Identify Primary Contamination Points.

    abstract::Significant knowledge gaps exist in our understanding of Campylobacter jejuni contamination of the poultry production continuum. Microbiological surveillance and genotypic characterization were undertaken on C. jejuni isolates longitudinally recovered from three poultry farms (weekly samples), the abattoir at which bi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02001-20

    authors: Inglis GD,Ramezani N,Taboada EN,Boras VF,Uwiera RRE

    更新日期:2021-01-15 00:00:00

  • Thermal destruction of Clostridium botulinum spores suspended in tomato juice in aluminum thermal death time tubes.

    abstract::The heat destruction characteristics of Clostridium botulinum spores suspended in tomato juice and phosphate buffer were determined by the survivor curve method with aluminum thermal death time tubes. Two type A strains of C. botulinum and a type B strain were evaluated. Strains A16037 and B15580 were implicated in ou...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.34.1.23-29.1977

    authors: Odlaug TE,Pflug IJ

    更新日期:1977-07-01 00:00:00

  • Coprinus comatus damages nematode cuticles mechanically with spiny balls and produces potent toxins to immobilize nematodes.

    abstract::We reported recently a unique fungal structure, called the spiny ball, on the vegetative hyphae of Coprinus comatus (O. F. Müll.:Fr.) Pers. Although some observations regarding the role of this structure were presented, its function remained largely unknown. In this study, we showed that purified (isolated and washed)...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02770-06

    authors: Luo H,Liu Y,Fang L,Li X,Tang N,Zhang K

    更新日期:2007-06-01 00:00:00

  • Degradation and debittering of a tryptic digest from beta-casein by aminopeptidase N from Lactococcus lactis subsp. cremoris Wg2.

    abstract::The mode of action of purified aminopeptidase N from Lactococcus lactis subsp. cremoris Wg2 on a complex peptide mixture of a tryptic digest from bovine beta-casein was analyzed. The oligopeptides produced in the tryptic digest before and after aminopeptidase N treatment were identified by analysis of the N- and C-ter...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.5.1430-1436.1993

    authors: Tan PS,van Kessel TA,van de Veerdonk FL,Zuurendonk PF,Bruins AP,Konings WN

    更新日期:1993-05-01 00:00:00

  • Effect of 3-Phenylpropanoic Acid on Capsule and Cellulases of Ruminococcus albus 8.

    abstract::The morphology and cellulases of Ruminococcus albus 8 were markedly affected by the inclusion of 3-phenylpropanoic acid (PPA) in a defined growth medium. PPA-grown bacteria produced substantial quantities of cell-bound cellulase, as well as a very high-molecular-weight extracellular enzyme and lesser amounts of two lo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.48.1.218-223.1984

    authors: Stack RJ,Hungate RE

    更新日期:1984-07-01 00:00:00

  • Legionella contamination of dental-unit waters.

    abstract::Volume 61, no. 4, p. 1211, column 2, line 40: this sentence should read as follows. "The viable-culture methods for Legionella sp. detection, however, often fail, and the Centers for Disease Control and Prevention has turned, when necessary, to PCR for epidemiological investigations of Legionnaires' disease and Pontia...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:

    authors: Atlas RM,Williams JF,Huntington MK

    更新日期:1996-04-01 00:00:00

  • Use of yeast spores for microencapsulation of enzymes.

    abstract::Here, we report a novel method to produce microencapsulated enzymes using Saccharomyces cerevisiae spores. In sporulating cells, soluble secreted proteins are transported to the spore wall. Previous work has shown that the spore wall is capable of retaining soluble proteins because its outer layers work as a diffusion...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00153-14

    authors: Shi L,Li Z,Tachikawa H,Gao XD,Nakanishi H

    更新日期:2014-08-01 00:00:00

  • shift from acetoclastic to H2-dependent methanogenesis in a west Siberian peat bog at low pH values and isolation of an acidophilic Methanobacterium strain.

    abstract::Methane production and archaeal community composition were studied in samples from an acidic peat bog incubated at different temperatures and pH values. H(2)-dependent methanogenesis increased strongly at the lowest pH, 3.8, and Methanobacteriaceae became important except for Methanomicrobiaceae and Methanosarcinaceae...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02413-06

    authors: Kotsyurbenko OR,Friedrich MW,Simankova MV,Nozhevnikova AN,Golyshin PN,Timmis KN,Conrad R

    更新日期:2007-04-01 00:00:00

  • Biogeochemical Conditions Favoring Magnetite Formation during Anaerobic Iron Reduction.

    abstract::Several anaerobic bacteria isolated from the sediments of Contrary Creek, an iron-rich environment, produced magnetite when cultured in combinations but not when cultured alone in synthetic iron oxyhydroxide medium. When glucose was added as a carbon source, the pH of the medium decreased (to 5.5) and no magnetite was...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.11.2610-2616.1987

    authors: Bell PE,Mills AL,Herman JS

    更新日期:1987-11-01 00:00:00

  • Isolation and characterization of "Methanosphaera cuniculi" sp. nov.

    abstract::A nonmotile, gram-positive, spherical organism was isolated from the intestinal tracts of rabbits. Both hydrogen and methanol were required for growth. No methane was produced from hydrogen-carbon dioxide, formate, acetate, methylamines, ethanol, or isopropanol. The optimum pH was 6.8, and the optimum temperature was ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.3.768-771.1988

    authors: Biavati B,Vasta M,Ferry JG

    更新日期:1988-03-01 00:00:00

  • Rectal administration of Escherichia coli O157:H7: novel model for colonization of ruminants.

    abstract::Escherichia coli O157:H7 causes hemorrhagic colitis and life-threatening complications. Because healthy cattle are reservoirs for the bacterium, ruminant infection models have applications in analyzing the relationship between cattle and this human pathogen and in testing interventions to reduce or prevent bovine colo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.8.4588-4595.2004

    authors: Sheng H,Davis MA,Knecht HJ,Hovde CJ

    更新日期:2004-08-01 00:00:00

  • Trifolitoxin Production Increases Nodulation Competitiveness of Rhizobium etli CE3 under Agricultural Conditions.

    abstract::A major barrier to the use of nitrogen-fixing inoculum strains for the enhancement of legume productivity is the inability of commercially available strains to compete with indigenous rhizobia for nodule formation. Despite extensive research on nodulation competitiveness, there are no examples of field efficacy studie...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.7.2630-2633.1998

    authors: Robleto EA,Kmiecik K,Oplinger ES,Nienhuis J,Triplett EW

    更新日期:1998-07-01 00:00:00

  • Molecular analysis of a Saccharomyces cerevisiae mutant with improved ability to utilize xylose shows enhanced expression of proteins involved in transport, initial xylose metabolism, and the pentose phosphate pathway.

    abstract::Differences between the recombinant xylose-utilizing Saccharomyces cerevisiae strain TMB 3399 and the mutant strain TMB 3400, derived from TMB 3399 and displaying improved ability to utilize xylose, were investigated by using genome-wide expression analysis, physiological characterization, and biochemical assays. Samp...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.2.740-746.2003

    authors: Wahlbom CF,Cordero Otero RR,van Zyl WH,Hahn-Hägerdal B,Jönsson LJ

    更新日期:2003-02-01 00:00:00

  • Degradation of a nonylphenol single isomer by Sphingomonas sp. strain TTNP3 leads to a hydroxylation-induced migration product.

    abstract::Sphingomonas sp. strain TTNP3 degrades 4(3',5'-dimethyl-3'-heptyl)-phenol and unidentified metabolites that were described previously. The chromatographic analyses of the synthesized reference compound and the metabolites led to their identification as 2(3',5'-dimethyl-3'-heptyl)-1,4-benzenediol. This finding indicate...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.11.6897-6900.2004

    authors: Corvini PF,Meesters RJ,Schäffer A,Schröder HF,Vinken R,Hollender J

    更新日期:2004-11-01 00:00:00

  • Chloromethane utilization gene cluster from Hyphomicrobium chloromethanicum strain CM2(T) and development of functional gene probes to detect halomethane-degrading bacteria.

    abstract::Hyphomicrobium chloromethanicum CM2(T), an aerobic methylotrophic member of the alpha subclass of the class proteobacteria, can grow with chloromethane as the sole carbon and energy source. H. chloromethanicum possesses an inducible enzyme system for utilization of chloromethane, in which two polypeptides (67-kDa CmuA...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.1.307-316.2001

    authors: McAnulla C,Woodall CA,McDonald IR,Studer A,Vuilleumier S,Leisinger T,Murrell JC

    更新日期:2001-01-01 00:00:00

  • Distribution and survival of motile Aeromonas spp. in brackish water receiving sewage treatment effluent.

    abstract::The spatiotemporal distributions of Aeromonas spp. and fecal coliforms in a cove receiving sewage treatment effluent and draining into a brackish lagoon were studied for 34 months with sampling at six stations. A total of 452 strains of Aeromonas spp. were isolated and identified at the outflow of the treatment system...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.9.2459-2467.1991

    authors: Monfort P,Baleux B

    更新日期:1991-09-01 00:00:00

  • The bactericidal activity of pediocin PA-1 is specifically inhibited by a 15-mer fragment that spans the bacteriocin from the center toward the C terminus.

    abstract::A 15-mer peptide fragment derived from pediocin PA-1 (from residue 20 to residue 34) specifically inhibited the bactericidal activity of pediocin PA-1. The fragment did not inhibit the pediocin-like bacteriocins sakacin P, leucocin A, and curvacin A to nearly the same extent as it inhibited pediocin PA-1. Enterocin A,...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.12.5057-5060.1998

    authors: Fimland G,Jack R,Jung G,Nes IF,Nissen-Meyer J

    更新日期:1998-12-01 00:00:00

  • Microbial conversion of glycerol to 1,3-propanediol: physiological comparison of a natural producer, Clostridium butyricum VPI 3266, and an engineered strain, Clostridium acetobutylicum DG1(pSPD5).

    abstract::Clostridium acetobutylicum is not able to grow on glycerol as the sole carbon source since it cannot reoxidize the excess of NADH generated by glycerol catabolism. Nevertheless, when the pSPD5 plasmid, carrying the NADH-consuming 1,3-propanediol pathway from C. butyricum VPI 3266, was introduced into C. acetobutylicum...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.72.1.96-101.2006

    authors: González-Pajuelo M,Meynial-Salles I,Mendes F,Soucaille P,Vasconcelos I

    更新日期:2006-01-01 00:00:00

  • Involvement of the Cell Wall Integrity Pathway of Saccharomyces cerevisiae in Protection against Cadmium and Arsenate Stresses.

    abstract::Contamination of soil and water with heavy metals and metalloids is a serious environmental problem. Cadmium and arsenic are major environmental contaminants that pose a serious threat to human health. Although toxicities of cadmium and arsenic to living organisms have been extensively studied, the molecular mechanism...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01339-20

    authors: Techo T,Charoenpuntaweesin S,Auesukaree C

    更新日期:2020-10-15 00:00:00

  • Deterioration-Associated Microbiome of Stone Monuments: Structure, Variation, and Assembly.

    abstract::Research on the microbial communities that colonize stone monuments may provide a new understanding of stone biodeterioration and microbe-induced carbonate precipitation. This work investigated the seasonal variation of microbial communities in 2016 and 2017, as well as its effects on stone monuments. We determined th...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02680-17

    authors: Li Q,Zhang B,Yang X,Ge Q

    更新日期:2018-03-19 00:00:00

  • Evaluation of nongermicidal handwashing protocols for removal of transient microbial flora.

    abstract::A method has been described which can compare the efficacy of different nongermicidal handwashing protocols for removal of transient microbial flora without the necessity of establishing or relying on a previously determined baseline for an individual subject. The wash effluent is collected, and colony counts for the ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.49.5.1067-1071.1985

    authors: Vesley D,Lillquist DR,Le CT

    更新日期:1985-05-01 00:00:00

  • Decomposition of blue-green algal (cyanobacterial) blooms in lake mendota, wisconsin.

    abstract::Decomposition of natural populations of Lake Mendota phytoplankton dominated by blue-green algae (cyanobacteria) was monitored by using oxygen uptake and disappearance of chlorophyll, algal volume (fluorescence microscopy), particulate protein, particulate organic carbon, and photosynthetic ability (CO(2) up-take). In...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.37.5.820-830.1979

    authors: Fallon RD,Brock TD

    更新日期:1979-05-01 00:00:00

  • Horizontal gene transfer to endogenous endophytic bacteria from poplar improves phytoremediation of toluene.

    abstract::Poplar, a plant species frequently used for phytoremediation of groundwater contaminated with organic solvents, was inoculated with the endophyte Burkholderia cepacia VM1468. This strain, whose natural host is yellow lupine, contains the pTOM-Bu61 plasmid coding for constitutively expressed toluene degradation. Nonino...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.12.8500-8505.2005

    authors: Taghavi S,Barac T,Greenberg B,Borremans B,Vangronsveld J,van der Lelie D

    更新日期:2005-12-01 00:00:00

  • Linked redox precipitation of sulfur and selenium under anaerobic conditions by sulfate-reducing bacterial biofilms.

    abstract::A biofilm-forming strain of sulfate-reducing bacteria (SRB), isolated from a naturally occurring mixed biofilm and identified by 16S rDNA analysis as a strain of Desulfomicrobium norvegicum, rapidly removed 200 micro M selenite from solution during growth on lactate and sulfate. Elemental selenium and elemental sulfur...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.12.7063-7072.2003

    authors: Hockin SL,Gadd GM

    更新日期:2003-12-01 00:00:00

  • A method for DNA extraction from the desert cyanobacterium chroococcidiopsis and its application to identification of ftsZ

    abstract::A method was developed for extraction of DNA from Chroococcidiopsis that overcomes obstacles posed by bacterial contamination and the presence of a thick envelope surrounding the cyanobacterial cells. The method is based on the resistance of Chroococcidiopsis to lysozyme and consists of a lysozyme treatment followed b...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.10.4053-4056.1998

    authors: Billi D,Grilli Caiola M,Paolozzi L,Ghelardini P

    更新日期:1998-10-01 00:00:00

  • Comparison of genotypes and serotypes of Campylobacter jejuni isolated from Danish wild mammals and birds and from broiler flocks and humans.

    abstract::The incidence of human infection with Campylobacter jejuni is increasing in most developed countries and the reason for this is largely unknown. Although poultry meat is considered to be a major source, it is evident that other reservoirs exist, possibly common to humans and poultry. Environmental sources are believed...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.7.3115-3121.2001

    authors: Petersen L,Nielsen EM,Engberg J,On SL,Dietz HH

    更新日期:2001-07-01 00:00:00

  • Evidence of hydrolytic route for anaerobic cyanide degradation.

    abstract::Products observed during anaerobic cyanide transformation are consistent with a hydrolytic pathway (HCN + H2O <--> HCONH2 + H2O <--> HCOOH + NH3). Formate, the most frequently observed product, was generally converted to bicarbonate. Formamide was rapidly hydrolyzed to formate upon exposure to the anaerobic consortium...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.9.3163-3164.1992

    authors: Fallon RD

    更新日期:1992-09-01 00:00:00

  • Mechanism for oxidation of high-molecular-weight substrates by a fungal versatile peroxidase, MnP2.

    abstract::Unlike general peroxidases, Pleurotus ostreatus MnP2 was reported to have a unique property of direct oxidization of high-molecular-weight compounds, such as Poly R-478 and RNase A. To elucidate the mechanism for oxidation of polymeric substrates by MnP2, a series of mutant enzymes were produced by using a homologous ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02080-07

    authors: Tsukihara T,Honda Y,Sakai R,Watanabe T,Watanabe T

    更新日期:2008-05-01 00:00:00

  • High rates of conjugation in bacterial biofilms as determined by quantitative in situ analysis.

    abstract::Quantitative in situ determination of conjugative gene transfer in defined bacterial biofilms using automated confocal laser scanning microscopy followed by three-dimensional analysis of cellular biovolumes revealed conjugation rates 1,000-fold higher than those determined by classical plating techniques. Conjugation ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.8.3710-3713.1999

    authors: Hausner M,Wuertz S

    更新日期:1999-08-01 00:00:00