Degradation and debittering of a tryptic digest from beta-casein by aminopeptidase N from Lactococcus lactis subsp. cremoris Wg2.

abstract：:Human sapovirus sequences were identified in 12 (100%) influent and 7 (58%) effluent wastewater samples collected once a month for a year. The strains were characterized based on their partial capsid gene sequences and classified into 10 genotypes, demonstrating that genetically diverse sapovirus strains infect humans...

journal_title：Applied and environmental microbiology

authors： Kitajima M,Haramoto E,Phanuwan C,Katayama H

更新日期：2011-06-01 00:00:00

abstract：:This paper presents a method for determining mutagenic concentrations of chemicals by using an agar diffusion assay. The method is based on the linear relationship between the amount of chemical placed at the center of the dish and the radius of the mutagenic zone. A brief theoretical discussion and experimental data ...

journal_title：Applied and environmental microbiology

authors： Awerbuch TE,Stark AA

更新日期：1979-12-01 00:00:00

abstract：:Previous studies have demonstrated that reduced humic substances (HS) can be reoxidized by anaerobic bacteria such as Geobacter, Geothrix, and Wolinella species with a suitable electron acceptor; however, little is known of the importance of this metabolism in the environment. Recently we investigated this metabolism ...

journal_title：Applied and environmental microbiology

authors： Coates JD,Cole KA,Chakraborty R,O'Connor SM,Achenbach LA

更新日期：2002-05-01 00:00:00

abstract：:An important feature of microbial biofilms is the development of four-dimensional physical and chemical gradients in space and time. There is need for novel approaches to probe these so-called microenvironments to determine their effect on biofilm-specific processes. In this study, we describe the use of seminaphthorh...

journal_title：Applied and environmental microbiology

authors： Hunter RC,Beveridge TJ

更新日期：2005-05-01 00:00:00

abstract：:Cellobiose labeled either at the reducing end (4-O-beta-d-glucopyranosyl-d-[U-C]glucopyranose) or at the nonreducing end (4-O-beta-d-[U-C]glucopyranosyl-d -glucopyranose) was synthesized with Clostridium thermocellum cellobiose phosphorylase at greater than 98% purity. The radioactive cellobioses were identical to aut...

journal_title：Applied and environmental microbiology

authors： Ng TK,Zeikus JG

更新日期：1986-10-01 00:00:00

abstract：:Lactobacillus plantarum is an attractive candidate for bioprocessing of lignocellulosic biomass due to its high metabolic variability, including its ability to ferment both pentoses and hexoses, as well as its high acid tolerance, a quality often utilized in industrial processes. This bacterium grows naturally on biom...

journal_title：Applied and environmental microbiology

authors： Moraïs S,Shterzer N,Grinberg IR,Mathiesen G,Eijsink VG,Axelsson L,Lamed R,Bayer EA,Mizrahi I

更新日期：2013-09-01 00:00:00

abstract：:Recent outbreaks of food-borne illness associated with the consumption of produce have increased concern over wildlife reservoirs of food-borne pathogens. Wild rodents are ubiquitous, and those living close to agricultural farms may pose a food safety risk should they shed zoonotic microorganisms in their feces near o...

journal_title：Applied and environmental microbiology

authors： Kilonzo C,Li X,Vivas EJ,Jay-Russell MT,Fernandez KL,Atwill ER

更新日期：2013-10-01 00:00:00

abstract：:Bacteroidales are attractive as water quality indicators because of their potential to discern sources of fecal pollution, and it is presumed that these bacteria do not multiply outside their host organisms. The persistence of a fecal Bacteroidales marker was monitored over 14 days in river water microcosms that varie...

journal_title：Applied and environmental microbiology

authors： Schulz CJ,Childers GW

更新日期：2011-04-01 00:00:00

abstract：:Chitin is an abundant biopolymer whose degradation is mediated primarily by bacterial chitinases. We developed a degenerate PCR primer set to amplify a approximately 900-bp fragment of family 18, group I chitinase genes and used it to retrieve these gene fragments from environmental samples. Clone libraries of presump...

journal_title：Applied and environmental microbiology

authors： LeCleir GR,Buchan A,Hollibaugh JT

更新日期：2004-12-01 00:00:00

abstract：:Most denitrifiers produce nitrous oxide (N(2)O) instead of dinitrogen (N(2)) under aerobic conditions. We isolated and characterized novel aerobic denitrifiers that produce low levels of N(2)O under aerobic conditions. We monitored the denitrification activities of two of the isolates, strains TR2 and K50, in batch an...

journal_title：Applied and environmental microbiology

authors： Takaya N,Catalan-Sakairi MA,Sakaguchi Y,Kato I,Zhou Z,Shoun H

更新日期：2003-06-01 00:00:00

abstract：:Salivaricin A (SalA), the first Streptococcus salivarius lantibiotic to be characterized, appears to be inhibitory to most Streptococcus pyogenes strains. A variant of the SalA structural gene (salA1) is present in more than 90% of S. pyogenes strains, but only strains of M serotype 4 and T pattern 4 produce the biolo...

journal_title：Applied and environmental microbiology

authors： Wescombe PA,Upton M,Dierksen KP,Ragland NL,Sivabalan S,Wirawan RE,Inglis MA,Moore CJ,Walker GV,Chilcott CN,Jenkinson HF,Tagg JR

更新日期：2006-02-01 00:00:00

abstract：:Upstream of the Streptomyces coelicolor A3(2) chitinase G gene, a small gene (named chb3) is located whose deduced product shares 37% identical amino acids with the previously described CHB1 protein from Streptomyces olivaceoviridis. The chb3 gene and its upstream region were cloned in a multicopy vector and transform...

journal_title：Applied and environmental microbiology

authors： Saito A,Miyashita K,Biukovic G,Schrempf H

更新日期：2001-03-01 00:00:00

abstract：:The food-borne pathogenic bacterium Listeria is known for relatively low morbidity and high mortality rates, reaching up to 25 to 30%. Listeria is a hardy organism, and its control in foods represents a significant challenge. Many naturally occurring compounds, including the bacteriocin nisin and a number of plant ess...

journal_title：Applied and environmental microbiology

authors： Field D,Daly K,O'Connor PM,Cotter PD,Hill C,Ross RP

更新日期：2015-04-01 00:00:00

abstract：:Campylobacter jejuni is a foodborne pathogen that is recognized as the leading cause of human bacterial gastroenteritis. The widespread use of antibiotics in medicine and in animal husbandry has led to an increased incidence of antibiotic resistance in Campylobacter In addition to a role in multidrug resistance (MDR),...

journal_title：Applied and environmental microbiology

authors： Vieira A,Ramesh A,Seddon AM,Karlyshev AV

更新日期：2017-10-31 00:00:00

abstract：:Microcystis aeruginosa is a common cyanobacterium in water blooms that appear widely in nutrient-rich, fresh, and brackish waters, and its toxic blooms cause the death of domestic animals. The administration of a crude toxic cell extract of M. aeruginosa K-139 to mice can produce tumor necrosis factor (TNF) and prompt...

journal_title：Applied and environmental microbiology

authors： Nakano Y,Shirai M,Mori N,Nakano M

更新日期：1991-01-01 00:00:00

abstract：:Copper appears to be influencing the distribution and abundance of phytoplankton in marine environments, and cyanobacteria are thought to be the most sensitive of the phytoplankton groups to copper toxicity. By using growth assays of phylogenetically divergent clades, we found that coastal strains of marine Synechococ...

journal_title：Applied and environmental microbiology

authors： Stuart RK,Dupont CL,Johnson DA,Paulsen IT,Palenik B

更新日期：2009-08-01 00:00:00

abstract：:Examination of Listeria monocytogenes prevalence among ready-to-eat foods in Japan revealed frequent (5.7 to 12.1%) contamination of minced tuna and fish roe products, and the isolates had the same virulence levels as clinical isolates in terms of invasion efficiency and infectivity in cell cultures and a murine infec...

journal_title：Applied and environmental microbiology

authors： Miya S,Takahashi H,Ishikawa T,Fujii T,Kimura B

更新日期：2010-05-01 00:00:00

abstract：:Aspergillus niger JTS 191 was selected from many microorganisms tested as capable of converting ionones to other compounds having aromas. The individual transformation products from beta-ionone were isolated and identified by comparison with synthetically derived compounds. The major products were (R)-4-hydroxy-beta-i...

journal_title：Applied and environmental microbiology

authors： Mikami Y,Fukunaga Y,Arita M,Kisaki T

更新日期：1981-03-01 00:00:00

abstract：:This study was performed to determine the effects of feeding a fiber-rich fraction of Brassica vegetables on the immune response through changes in enteric bacteria and short-chain fatty acid (SCFA) production in normal mice. The boiled-water-insoluble fraction of Brassica rapa L. (nozawana), which consists mainly of ...

journal_title：Applied and environmental microbiology

authors： Tanaka S,Yamamoto K,Yamada K,Furuya K,Uyeno Y

更新日期：2016-04-18 00:00:00

abstract：:High-cell-density fermentation for industrial production of chemicals can impose numerous stresses on cells due to high substrate, product, and by-product concentrations; high osmolarity; reactive oxygen species; and elevated temperatures. There is a need to develop platform strains of industrial microorganisms that a...

journal_title：Applied and environmental microbiology

authors： Lennen RM,Herrgård MJ

更新日期：2014-10-01 00:00:00

abstract：:The significance of dissolved combined amino acids (DCAA), dissolved free amino acids (DFAA), and dissolved DNA (D-DNA) as sources of C and N for marine bacteria in batch cultures with variable substrate C/N ratios was studied. Glucose, ammonium, alanine, and phosphate were added to the cultures to produce C/N ratios ...

journal_title：Applied and environmental microbiology

authors： Jørgensen NO,Kroer N,Coffin RB

更新日期：1994-11-01 00:00:00

abstract：:The methylotrophic yeast Pichia pastoris has been utilized for heterologous protein expression for over 30 years. Because P. pastoris secretes few of its own proteins, the exported recombinant protein is the major polypeptide in the extracellular medium, making purification relatively easy. Unfortunately, some recombi...

journal_title：Applied and environmental microbiology

authors： Naranjo CA,Jivan AD,Vo MN,de Sa Campos KH,Deyarmin JS,Hekman RM,Uribe C,Hang A,Her K,Fong MM,Choi JJ,Chou C,Rabara TR,Myers G,Moua P,Thor,Risser DD,Vierra CA,Franz AH,Lin-Cereghino J,Lin-Cereghino GP

更新日期：2019-11-27 00:00:00

abstract：:A new method is described in which the Salkowski reaction is used for the rapid selection of alkaloid-producing mutants of the ergot fungus. This method was used to investigate the influence of a second mutation with N-methyl-N'-nitro-N-nitrosoguanidine (NTG) on various mutants selected by a preliminary NTG mutation o...

journal_title：Applied and environmental microbiology

authors： Srikrai S,Robbers JE

更新日期：1983-04-01 00:00:00

abstract：:Production of propionicin PLG-1 by Propionibacterium thoenii P127 was pH dependent, with maximal activity detected in supernatants of cultures grown at pH 7.0 Propionicin PLG-1 was purified by ion-exchange chromatography and isoelectric focusing. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of propionicin...

journal_title：Applied and environmental microbiology

authors： Lyon WJ,Glatz BA

更新日期：1993-01-01 00:00:00

abstract：:Alcaligenes eutrophus was grown autotrophically in 23-liter batch cultures in a controlled H2-O2-CO2 atmosphere. It was demonstrated that the need for periodic supplements of individual nutrients could be anticipated before cell growth depleted these nutrients to the point of becoming growth rate limiting. As a result...

journal_title：Applied and environmental microbiology

authors： Repaske R,Mayer R

更新日期：1976-10-01 00:00:00

abstract：:It has long been assumed that prolonged holding of environmental samples at the ambient air temperature prior to bacteriological analysis is detrimental to isolation and detection of Vibrio cholerae, the causative agent of pandemic cholera. The present study was aimed at understanding the effect of transporting enviro...

journal_title：Applied and environmental microbiology

authors： Alam M,Sadique A,Nur-A-Hasan,Bhuiyan NA,Nair GB,Siddique AK,Sack DA,Ahsan S,Huq A,Sack RB,Colwell RR

更新日期：2006-03-01 00:00:00

abstract：:A real-time immunoquantitative PCR (iqPCR) method for detection of Staphylococcus aureus enterotoxin B (SEB) was developed and evaluated using both pure cultures and foods. The assay consisted of immunocapture of SEB and real-time PCR amplification of the DNA probe linked to the detection antibody. iqPCR was compared ...

journal_title：Applied and environmental microbiology

authors： Rajkovic A,El-Moualij B,Uyttendaele M,Brolet P,Zorzi W,Heinen E,Foubert E,Debevere J

更新日期：2006-10-01 00:00:00

abstract：:Research on the microbial communities that colonize stone monuments may provide a new understanding of stone biodeterioration and microbe-induced carbonate precipitation. This work investigated the seasonal variation of microbial communities in 2016 and 2017, as well as its effects on stone monuments. We determined th...

journal_title：Applied and environmental microbiology

authors： Li Q,Zhang B,Yang X,Ge Q

更新日期：2018-03-19 00:00:00

abstract：:Pellet softening reactors are used in centralized and decentralized drinking water treatment plants for the removal of calcium (hardness) through chemically induced precipitation of calcite. This is accomplished in fluidized pellet reactors, where a strong base is added to the influent to increase the pH and facilitat...

journal_title：Applied and environmental microbiology

authors： Hammes F,Boon N,Vital M,Ross P,Magic-Knezev A,Dignum M

更新日期：2011-02-01 00:00:00

abstract：:Two 16S rRNA-targeted oligonucleotide probes were designed for the archaeal kingdoms Euryachaeota and Crenarchaeota. Probe specificities were evaluated by nonradioactive dot blot hybridization against selected reference organisms. The successful application of fluorescent-probe derivatives for whole-cell hybridization...

journal_title：Applied and environmental microbiology

authors： Burggraf S,Mayer T,Amann R,Schadhauser S,Woese CR,Stetter KO

更新日期：1994-09-01 00:00:00