Application of a pH-sensitive fluoroprobe (C-SNARF-4) for pH microenvironment analysis in Pseudomonas aeruginosa biofilms.

Abstract:

:An important feature of microbial biofilms is the development of four-dimensional physical and chemical gradients in space and time. There is need for novel approaches to probe these so-called microenvironments to determine their effect on biofilm-specific processes. In this study, we describe the use of seminaphthorhodafluor-4F 5-(and-6) carboxylic acid (C-SNARF-4) for pH microenvironment analysis in Pseudomonas aeruginosa biofilms. C-SNARF-4 is a fluorescent ratiometric probe that allows pH quantification independent of probe concentration and/or laser intensity. By confocal scanning laser microscopy, C-SNARF-4 revealed pH heterogeneity throughout the biofilm in both the x,y and x,z planes, with values ranging from pH 5.6 (within the biofilm) to pH 7.0 (bulk fluid). pH values were typically remarkably different than those just a few micrometers away. Although this probe has been successfully used in a number of eukaryotic systems, problems have been reported which describe spectral emission changes as a result of macromolecular interactions with the fluorophore. To assess how the biofilm environment may influence fluorescent properties of the dye, fluorescence of C-SNARF-4 was quantified via spectrofluorometry while the probe was suspended in various concentrations of representative biofilm matrix components (i.e., proteins, polysaccharides, and bacterial cells) and growth medium. Surprisingly, our data demonstrate that few changes in emission spectra occur as a result of matrix interactions below pH 7. These studies suggest that C-SNARF-4 can be used as a reliable indicator of pH microenvironments, which may help elucidate their influence on the medical and geobiological roles of natural biofilms.

journal_name

Appl Environ Microbiol

authors

Hunter RC,Beveridge TJ

doi

10.1128/AEM.71.5.2501-2510.2005

keywords:

subject

Has Abstract

pub_date

2005-05-01 00:00:00

pages

2501-10

issue

5

eissn

0099-2240

issn

1098-5336

pii

71/5/2501

journal_volume

71

pub_type

杂志文章
  • Characterization of a T5-like coliphage, SPC35, and differential development of resistance to SPC35 in Salmonella enterica serovar typhimurium and Escherichia coli.

    abstract::The potential of bacteriophage as an alternative biocontrol agent has recently been revisited due to the widespread occurrence of antibiotic-resistant bacteria. We isolated a virulent bacteriophage, SPC35, that can infect both Salmonella enterica serovar Typhimurium and Escherichia coli. Morphological analysis by tran...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02504-10

    authors: Kim M,Ryu S

    更新日期:2011-03-01 00:00:00

  • Factors and selenocysteine insertion sequence requirements for the synthesis of selenoproteins from a gram-positive anaerobe in Escherichia coli.

    abstract::Selenoprotein synthesis in Escherichia coli strictly depends on the presence of a specific selenocysteine insertion sequence (SECIS) following the selenocysteine-encoding UGA codon of the respective mRNA. It is recognized by the selenocysteine-specific elongation factor SelB, leading to cotranslational insertion of se...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02238-07

    authors: Gursinsky T,Gröbe D,Schierhorn A,Jäger J,Andreesen JR,Söhling B

    更新日期:2008-03-01 00:00:00

  • Variability of total and pathogenic Vibrio parahaemolyticus densities in northern Gulf of Mexico water and oysters.

    abstract::Vibrio parahaemolyticus is indigenous to coastal environments and a frequent cause of seafood-borne gastroenteritis in the United States, primarily due to raw-oyster consumption. Previous seasonal-cycle studies of V. parahaemolyticus have identified water temperature as the strongest environmental predictor. Salinity ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01700-07

    authors: Zimmerman AM,DePaola A,Bowers JC,Krantz JA,Nordstrom JL,Johnson CN,Grimes DJ

    更新日期:2007-12-01 00:00:00

  • 19F nuclear magnetic resonance analysis of 5-fluorouracil metabolism in wild-type and 5-fluorouracil-resistant Nectria haematococca.

    abstract::A mutant (furA3) was isolated from the S1 wild-type strain of Nectria haematococca on the basis of its resistance to 5-fluorouracil (5FU). This mutant has greatly reduced activity of uracil phosphoribosyltransferase, a pyrimidine salvage enzyme catalyzing the synthesis of UMP from uracil. The metabolism of 5FU was exa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.10.2474-2479.1989

    authors: Parisot D,Malet-Martino MC,Crasnier P,Martino R

    更新日期:1989-10-01 00:00:00

  • Temperature affects species distribution in symbiotic populations of Vibrio spp.

    abstract::The genus Sepiola (Cephalopoda: Sepiolidae) contains 10 known species that occur in the Mediterranean Sea today. All Sepiola species have a light organ that contains at least one of two species of luminous bacteria, Vibrio fischeri and Vibrio logei. The two Vibrio species coexist in at least four Sepiola species (S. a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.8.3550-3555.2000

    authors: Nishiguchi MK

    更新日期:2000-08-01 00:00:00

  • Characterization of a novel type II restriction-modification system, Sth368I, encoded by the integrative element ICESt1 of Streptococcus thermophilus CNRZ368.

    abstract::A novel type II restriction and modification (R-M) system, Sth368I, which confers resistance to phiST84, was found in Streptococcus thermophilus CNRZ368 but not in the very closely related strain A054. Partial sequencing of the integrative conjugative element ICESt1, carried by S. thermophilus CNRZ368 but not by A054,...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.4.1522-1528.2001

    authors: Burrus V,Bontemps C,Decaris B,Guédon G

    更新日期:2001-04-01 00:00:00

  • Role of Hippoboscidae flies as potential vectors of Bartonella spp. infecting wild and domestic ruminants.

    abstract::The putative role of biting flies in Bartonella transmission among ruminants was investigated. Amplification of the Bartonella citrate synthase gene from 83 Hippoboscidae was detected in 94% of 48 adult Lipoptena cervi flies, 71% of 17 adult Hippobosca equina flies, 100% of 20 adult Melophagus ovinus flies, and 100% o...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.10.6302-6305.2004

    authors: Halos L,Jamal T,Maillard R,Girard B,Guillot J,Chomel B,Vayssier-Taussat M,Boulouis HJ

    更新日期:2004-10-01 00:00:00

  • Massive infection of seabird ticks with bacterial species related to Coxiella burnetii.

    abstract::Seabird ticks are known reservoirs of bacterial pathogens of medical importance; however, ticks parasitizing tropical seabirds have received less attention than their counterparts from temperate and subpolar regions. Recently, Rickettsia africae was described to infect seabird ticks of the western Indian Ocean and New...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00477-14

    authors: Wilkinson DA,Dietrich M,Lebarbenchon C,Jaeger A,Le Rouzic C,Bastien M,Lagadec E,McCoy KD,Pascalis H,Le Corre M,Dellagi K,Tortosa P

    更新日期:2014-06-01 00:00:00

  • Taking the starch out of oral biofilm formation: molecular basis and functional significance of salivary α-amylase binding to oral streptococci.

    abstract::α-Amylase-binding streptococci (ABS) are a heterogeneous group of commensal oral bacterial species that comprise a significant proportion of dental plaque microfloras. Salivary α-amylase, one of the most abundant proteins in human saliva, binds to the surface of these bacteria via specific surface-exposed α-amylase-bi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1128/AEM.02581-12

    authors: Nikitkova AE,Haase EM,Scannapieco FA

    更新日期:2013-01-01 00:00:00

  • Responses of the anaerobic bacterial community to addition of organic C in chromium(VI)- and iron(III)-amended microcosms.

    abstract::Chromium (VI) is toxic to microorganisms and can inhibit the biodegradation of organic pollutants in contaminated soils. We used microcosms amended with either glucose or protein (to drive bacterial community change) and Fe(III) (to stimulate iron-reducing bacteria) to study the effect of various concentrations of Cr(...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.72.1.628-637.2006

    authors: Kourtev PS,Nakatsu CH,Konopka A

    更新日期:2006-01-01 00:00:00

  • Malonate catabolism does not drive N2 fixation in legume nodules.

    abstract::Malonyl-coenzyme A (CoA) decarboxylase, malonyl-CoA synthetase, and malonate transporter mutants of Rhizobium leguminosarum bv. viciae and trifolii fixed N2 at wild-type rates on pea and clover, respectively. Thus, malonate does not drive N2 fixation in legume nodules. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00919-13

    authors: Karunakaran R,East AK,Poole PS

    更新日期:2013-07-01 00:00:00

  • Use of Polymerase Chain Reaction To Detect the Take-All Fungus, Gaeumannomyces graminis, in Infected Wheat Plants.

    abstract::Gaeumannomyces graminis, the causative agent of take-all disease of wheat, barley, and oats, was detected in infected wheat seedlings by using the polymerase chain reaction to amplify Gaeumannomyces-specific DNA fragments. Nested primers and two rounds of amplification were used to amplify two fragments, approximately...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.2.553-556.1991

    authors: Schesser K,Luder A,Henson JM

    更新日期:1991-02-01 00:00:00

  • Extensive manipulation of caseicins A and B highlights the tolerance of these antimicrobial peptides to change.

    abstract::Caseicins A and B are low-molecular-weight antimicrobial peptides which are released by proteolytic digestion of sodium caseinate. Caseicin A (IKHQGLPQE) is a nine-amino-acid cationic peptide, and caseicin B (VLNENLLR) is a neutral eight-amino-acid peptide; both have previously been shown to exhibit antibacterial acti...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.07312-11

    authors: Norberg S,O'Connor PM,Stanton C,Ross RP,Hill C,Fitzgerald GF,Cotter PD

    更新日期:2012-04-01 00:00:00

  • Mycoplasma agalactiae Secretion of β-(1→6)-Glucan, a Rare Polysaccharide in Prokaryotes, Is Governed by High-Frequency Phase Variation.

    abstract:UNLABELLED:Mycoplasmas are minimal, wall-less bacteria but have retained the ability to secrete complex carbohydrate polymers that constitute a glycocalyx. In members of the Mycoplasma mycoides cluster, which are important ruminant pathogens, the glycocalyx includes both cell-attached and cell-free polysaccharides. Thi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00274-16

    authors: Gaurivaud P,Baranowski E,Pau-Roblot C,Sagné E,Citti C,Tardy F

    更新日期:2016-05-16 00:00:00

  • Thermal Inactivation of Mycobacterium avium subsp. paratuberculosis in Artificially Contaminated Milk by Direct Steam Injection.

    abstract:UNLABELLED:The efficiency of direct steam injection (DSI) at 105 °C for 3 s to inactivate Mycobacterium avium subsp. paratuberculosis in milk at a pilot-plant scale was investigated. Milk samples were artificially contaminated with M. avium subsp. paratuberculosis and also with cow fecal material naturally infected wit...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.04042-15

    authors: Peterz M,Butot S,Jagadeesan B,Bakker D,Donaghy J

    更新日期:2016-04-18 00:00:00

  • Production of trypsin inhibitor by a Cephalosporium sp.

    abstract::Conditions for the production of the trypsin inhibitor from Cephalosporium sp. KM 388 were investigated. Polypeptone-meat extract-glucose medium supported excellent production of the trypsin inhibitor. In this medium, polypeptone and meat extract were utilized both as carbon and nitrogen sources and as limiting substr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.35.4.631-635.1978

    authors: Tsuchiya K,Kimura T

    更新日期:1978-04-01 00:00:00

  • Development of a Defined Medium for Growth of Cercospora rosicola Passerini.

    abstract::A simple synthetic liquid medium containing a single amino acid, glucose, salts, trace metals, and thiamine was developed for cultivation of Cercospora rosicola Passerini. Thiamine was shown to be important to growth. Culture of C. rosicola Passerini in a chemically defined medium makes possible studies of (+)-abscisi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.41.1.334-336.1981

    authors: Norman SM,Maier VP,Echols LC

    更新日期:1981-01-01 00:00:00

  • Isolation of Thermus strains from hot composts (60 to 80 degrees C).

    abstract::High numbers (10(7) to 10(10) cells per g [dry weight]) of heterotrophic, gram-negative, rod-shaped, non-sporeforming, aerobic, thermophilic bacteria related to the genus Thermus were isolated from thermogenic composts at temperatures between 65 and 82 degrees C. These bacteria were present in different types of waste...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.5.1723-1727.1996

    authors: Beffa T,Blanc M,Lyon PF,Vogt G,Marchiani M,Fischer JL,Aragno M

    更新日期:1996-05-01 00:00:00

  • A Structurally Novel Chitinase from the Chitin-Degrading Hyperthermophilic Archaeon Thermococcus chitonophagus.

    abstract:UNLABELLED:A structurally novel chitinase, Tc-ChiD, was identified from the hyperthermophilic archaeon Thermococcus chitonophagus, which can grow on chitin as the sole organic carbon source. The gene encoding Tc-ChiD contains regions corresponding to a signal sequence, two chitin-binding domains, and a putative catalyt...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00319-16

    authors: Horiuchi A,Aslam M,Kanai T,Atomi H

    更新日期:2016-05-31 00:00:00

  • Comparison of acid mine drainage microbial communities in physically and geochemically distinct ecosystems.

    abstract::This study presents population analyses of microbial communities inhabiting a site of extreme acid mine drainage (AMD) production. The site is the inactive underground Richmond mine at Iron Mountain, Calif., where the weathering of a massive sulfide ore body (mostly pyrite) produces solutions with pHs of approximately...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.11.4962-4971.2000

    authors: Bond PL,Druschel GK,Banfield JF

    更新日期:2000-11-01 00:00:00

  • Effect of spatial differences in microbial activity, pH, and substrate levels on methanogenesis initiation in refuse.

    abstract::The initiation of methanogenesis in refuse occurs under high volatile fatty acid (VFA) concentration and low pH (5.5 to 6.25), which generally are reported to inhibit methanogenic Archaea. One hypothesized mechanism for the initiation of methanogenesis in refuse decomposition is the presence of pH-neutral niches withi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02349-10

    authors: Staley BF,de Los Reyes FL 3rd,Barlaz MA

    更新日期:2011-04-01 00:00:00

  • Isolation of strains belonging to the cosmopolitan Polynucleobacter necessarius cluster from freshwater habitats located in three climatic zones.

    abstract::More than 40 bacterial strains belonging to the cosmopolitan Polynucleobacter necessarius cluster (Betaproteobacteria) were isolated from a broad spectrum of freshwater habitats located in three climatic zones. Sequences affiliated with the freshwater P. necessarius cluster are among the most frequently detected in st...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.9.5248-5254.2003

    authors: Hahn MW

    更新日期:2003-09-01 00:00:00

  • Hemolymph Microbiomes of Three Aquatic Invertebrates as Revealed by a New Cell Extraction Method.

    abstract::Symbiotic microorganisms have been found in the hemolymph (blood) of many aquatic invertebrates, such as crabs, shrimp, and oysters. Hemolymph is a critical site in the host immune response. Currently, studies on hemolymph microorganisms are mostly performed with culture-dependent strategies using selective media (e.g...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02824-17

    authors: Zhang X,Sun Z,Zhang X,Zhang M,Li S

    更新日期:2018-04-02 00:00:00

  • Mediterranean fruit fly as a potential vector of bacterial pathogens.

    abstract::The Mediterranean fruit fly (Ceratitis capitata) is a cosmopolitan pest of hundreds of species of commercial and wild fruits. It is considered a major economic pest of commercial fruits in the world. Adult Mediterranean fruit flies feed on all sorts of protein sources, including animal excreta, in order to develop egg...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.7.4052-4056.2005

    authors: Sela S,Nestel D,Pinto R,Nemny-Lavy E,Bar-Joseph M

    更新日期:2005-07-01 00:00:00

  • A Nonautochthonous U.S. Strain of Vibrio parahaemolyticus Isolated from Chesapeake Bay Oysters Caused the Outbreak in Maryland in 2010.

    abstract:UNLABELLED:In the summer of 2010, Vibrio parahaemolyticus caused an outbreak in Maryland linked to the consumption of oysters. Strains isolated from both stool and oyster samples were indistinguishable by pulsed-field gel electrophoresis (PFGE). However, the oysters contained other potentially pathogenic V. parahaemoly...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00096-16

    authors: Haendiges J,Jones J,Myers RA,Mitchell CS,Butler E,Toro M,Gonzalez-Escalona N

    更新日期:2016-05-16 00:00:00

  • Multilocus simple sequence repeat markers for differentiating strains and evaluating genetic diversity of Xylella fastidiosa.

    abstract::A genome-wide search was performed to identify simple sequence repeat (SSR) loci among the available sequence databases from four strains of Xylella fastidiosa (strains causing Pierce's disease, citrus variegated chlorosis, almond leaf scorch, and oleander leaf scorch). Thirty-four SSR loci were selected for SSR prime...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.8.4888-4892.2005

    authors: Lin H,Civerolo EL,Hu R,Barros S,Francis M,Walker MA

    更新日期:2005-08-01 00:00:00

  • Culture-independent analysis of fecal enterobacteria in environmental samples by single-cell mRNA profiling.

    abstract::A culture-independent method called mRNA profiling has been developed for the analysis of fecal enterobacteria and their physiological status in environmental samples. This taxon-specific approach determines the single-cell content of selected gene transcripts whose abundance is either directly or inversely proportion...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.8.4432-4439.2004

    authors: Chen H,Ponniah G,Salonen N,Blum P

    更新日期:2004-08-01 00:00:00

  • Cocultivation of Legionella pneumophila and free-living amoebae.

    abstract::Studies of the interaction of Legionella pneumophila with free-living amoebae showed that Naegleria lovaniensis and Acanthamoeba royreba could use L. pneumophila as a sole food source. However, growth of the amoebae on nonnutrient agar plates seeded with L. pneumophila was slower than growth on nonnutrient agar plates...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.44.4.954-959.1982

    authors: Tyndall RL,Domingue EL

    更新日期:1982-10-01 00:00:00

  • Toolbox Approaches Using Molecular Markers and 16S rRNA Gene Amplicon Data Sets for Identification of Fecal Pollution in Surface Water.

    abstract::In this study, host-associated molecular markers and bacterial 16S rRNA gene community analysis using high-throughput sequencing were used to identify the sources of fecal pollution in environmental waters in Brisbane, Australia. A total of 92 fecal and composite wastewater samples were collected from different host g...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02032-15

    authors: Ahmed W,Staley C,Sadowsky MJ,Gyawali P,Sidhu JP,Palmer A,Beale DJ,Toze S

    更新日期:2015-10-01 00:00:00

  • Localization and visualization of a coxiella-type symbiont within the lone star tick, Amblyomma americanum.

    abstract::A Coxiella-type microbe occurs at 100% frequency in all Amblyomma americanum ticks thus far tested. Using laboratory-reared ticks free of other microbes, we identified the Amblyomma-associated Coxiella microbe in several types of tissue and at various stages of the life cycle of A. americanum by 16S rRNA gene sequenci...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00537-07

    authors: Klyachko O,Stein BD,Grindle N,Clay K,Fuqua C

    更新日期:2007-10-01 00:00:00