19F nuclear magnetic resonance analysis of 5-fluorouracil metabolism in wild-type and 5-fluorouracil-resistant Nectria haematococca.

abstract：:The environmental transport of Cryptosporidium spp. through combined sewer overflow (CSO) and the occurrence of several emerging human-pathogenic Cryptosporidium species in developing countries remain unclear. In this study, we collected 40 CSO samples and 40 raw wastewater samples from Shanghai, China, and examined t...

journal_title：Applied and environmental microbiology

authors： Huang C,Hu Y,Wang L,Wang Y,Li N,Guo Y,Feng Y,Xiao L

更新日期：2017-08-01 00:00:00

abstract：:We report an ex vivo method, which uses pieces of human skin excised during routine plastic surgery, for testing survival of hazardous pathogens. Using this procedure, we compared the survival of human herpesvirus 2 on human skin and on metal disks. At the physiological skin temperature of 32 degrees C, the half-life ...

journal_title：Applied and environmental microbiology

authors： Graham ML,Springthorpe VS,Sattar SA

更新日期：1996-11-01 00:00:00

abstract：:Two strains of Penicillium digitatum and one strain of Penicillium italicum were exposed to various levels of sorbic acid and potassium sorbate, and the MICs were determined. Selected strains of the molds were then repeatedly exposed to subinhibitory levels of the compounds to determine whether increased tolerance mig...

journal_title：Applied and environmental microbiology

authors： Schroeder LL,Bullerman LB

更新日期：1985-10-01 00:00:00

abstract：:A mutant of the peptide antibiotic nisin in which the dehydroalanine residue at position 5 has been replaced by an alanine has been produced and structurally characterized. It is shown to have activity very similar to that of wild-type nisin in inhibiting growth of Lactococcus lactis and Micrococcus luteus but is very...

journal_title：Applied and environmental microbiology

authors： Chan WC,Dodd HM,Horn N,Maclean K,Lian LY,Bycroft BW,Gasson MJ,Roberts GC

更新日期：1996-08-01 00:00:00

abstract：:A novel strain of Halomonas (Tc-202), which has the capability of removing Tc(VII) from solid- and aqueous-phase material aerobically, was isolated from the marine environment. Tc-202 removed 55% of the total 99Tc in solutions at 15 degrees C by reducing Tc(VII) to Tc(V), but other Halomonas strains did not. ...

journal_title：Applied and environmental microbiology

authors： Fujimoto K,Morita T

更新日期：2006-12-01 00:00:00

abstract：:We assayed the tolerance to solvents of three toluene-degrading Pseudomonas putida strains and Pseudomonas mendocina KR1 in liquid and soil systems. P. putida DOT-T1 tolerated concentrations of heptane, propylbenzene, octanol, and toluene of at least 10% (vol/vol), while P. putida F1 and EEZ15 grew well in the presenc...

journal_title：Applied and environmental microbiology

authors： Huertas MJ,Duque E,Marqués S,Ramos JL

更新日期：1998-01-01 00:00:00

abstract：:The role of protease produced by a heterotrophic bacterium during growth was investigated with Aeromonas salmonicida, the pathogen of fish furunculosis, strain A-7301 and its protease-deficient mutant NTG-1 induced by mutagenesis. Strain A-7301 produced extracellular protease in a mixed amino acid medium (composed of ...

journal_title：Applied and environmental microbiology

authors： Sakai DK

更新日期：1985-10-01 00:00:00

abstract：:Oxolinic acid (OA), a quinolone, inhibits the activity of DNA gyrase composed of GyrA and GyrB and shows antibacterial activity against Burkholderia glumae. Since B. glumae causes bacterial seedling rot and grain rot of rice, both of which are devastating diseases, the emergence of OA-resistant bacteria has important ...

journal_title：Applied and environmental microbiology

authors： Maeda Y,Kiba A,Ohnishi K,Hikichi Y

更新日期：2004-09-01 00:00:00

abstract：:DNA microarray analysis showed that yfiD, yggB, and yggE genes were up-regulated when superoxide dismutase (SOD)-deficient Escherichia coli IM303 (I4) was cultivated under the oxidative stress generated by photoexcited TiO(2), and pYFD, pYGB, and pYGE were constructed by inserting the respective genes into a pUC 19 ve...

journal_title：Applied and environmental microbiology

authors： Kim SY,Nishioka M,Hayashi S,Honda H,Kobayashi T,Taya M

更新日期：2005-05-01 00:00:00

abstract：:The Bacteria community composition in an acidic Sphagnum peat bog (pH 3.9 to 4.5) was characterized by a combination of 16S rRNA gene clone library analysis, rRNA-targeted fluorescence in situ hybridization (FISH), and cultivation. Among 84 environmental 16S rRNA gene clones, a set of only 16 cloned sequences was clos...

journal_title：Applied and environmental microbiology

authors： Dedysh SN,Pankratov TA,Belova SE,Kulichevskaya IS,Liesack W

更新日期：2006-03-01 00:00:00

abstract：:The oxidation of methane in anoxic marine sediments is thought to be mediated by a consortium of methane-consuming archaea and sulfate-reducing bacteria. In this study, we compared results of rRNA gene (rDNA) surveys and lipid analyses of archaea and bacteria associated with methane seep sediments from several differe...

journal_title：Applied and environmental microbiology

authors： Orphan VJ,Hinrichs KU,Ussler W 3rd,Paull CK,Taylor LT,Sylva SP,Hayes JM,Delong EF

更新日期：2001-04-01 00:00:00

abstract：:The yeast Torulaspora delbrueckii, which propagates as a haploid, was made into a diploid by treatment with dimethyl sulfoxide (DMSO) on the regeneration of protoplasts. The diploid state was stably inherited; the cell volume was three times that of the parent strain and the cellular DNA content was two times that of ...

journal_title：Applied and environmental microbiology

authors： Sasaki T,Ohshima Y

更新日期：1987-07-01 00:00:00

abstract：:A total of 247 clones of 16S rRNA genes from microorganisms captured by 0.2- and 0.1-microm-pore-size filters from sedimentary and granite rock aquifers were amplified and yielded 37 operational taxonomic units (OTUs). Fifteen OTUs captured by 0.1-microm-pore-size filters were affiliated with the candidate divisions O...

journal_title：Applied and environmental microbiology

authors： Miyoshi T,Iwatsuki T,Naganuma T

更新日期：2005-02-01 00:00:00

abstract：:Superoxide dismutase (SOD) activity was determined during the growth cycle of unheated and heat-injured cells of Staphylococcus aureus MF-31. SOd activity levels dropped in unheated cells during the lag phase, increased during logarithmic phase, and became constant in the stationary phase. Cells which were sublethally...

journal_title：Applied and environmental microbiology

authors： Bucker ER,Martin SE

更新日期：1981-03-01 00:00:00

abstract：:In this study, we combined metabolic reconstruction, growth assays, and metabolome and transcriptome analyses to obtain a global view of the sulfur metabolic network and of the response to sulfur availability in Brevibacterium aurantiacum. In agreement with the growth of B. aurantiacum in the presence of sulfate and c...

journal_title：Applied and environmental microbiology

authors： Forquin MP,Hébert A,Roux A,Aubert J,Proux C,Heilier JF,Landaud S,Junot C,Bonnarme P,Martin-Verstraete I

更新日期：2011-02-01 00:00:00

abstract：:This work reports on the identification and molecular characterization of a two-component regulatory system (2CRS), encoded by serRK, which is believed to control the expression of the ser(2003) locus in Bifidobacterium breve UCC2003. The ser(2003) locus consists of two genes, Bbr_1319 (sagA) and Bbr_1320 (serU), whic...

journal_title：Applied and environmental microbiology

authors： Alvarez-Martin P,O'Connell Motherway M,Turroni F,Foroni E,Ventura M,van Sinderen D

更新日期：2012-10-01 00:00:00

abstract：:Bacterial strains were isolated on the basis of their ability to proliferate in a minimal medium containing one of a series of lignin-related compounds as the sole carbon and energy source. These included the aromatic monomers guaiacol, vanillic and coumaric acids, a dimer and a trimer possessing the arylglycerol-beta...

journal_title：Applied and environmental microbiology

authors： Gonzalez B,Merino A,Almeida M,Vicña R

更新日期：1986-12-01 00:00:00

abstract：:Soils harbor enormously diverse bacterial populations, and soil bacterial communities can vary greatly in composition across space. However, our understanding of the specific changes in soil bacterial community structure that occur across larger spatial scales is limited because most previous work has focused on eithe...

journal_title：Applied and environmental microbiology

authors： Lauber CL,Hamady M,Knight R,Fierer N

更新日期：2009-08-01 00:00:00

abstract：:Listeria monocytogenes is a food-borne, opportunistic, bacterial pathogen causing a wide spectrum of diseases, including meningitis, septicemia, abortion, and gastroenteritis, in humans and animals. Among the 13 L. monocytogenes serovars described, human listeriosis is mostly associated with strains of serovars 4b, 1/...

journal_title：Applied and environmental microbiology

authors： Severino P,Dussurget O,Vêncio RZ,Dumas E,Garrido P,Padilla G,Piveteau P,Lemaître JP,Kunst F,Glaser P,Buchrieser C

更新日期：2007-10-01 00:00:00

abstract：:Rubber-degrading activity was found in the extracellular culture medium of Xanthomonas sp. strain 35Y which was grown on natural rubber latex. Natural rubber in the latex state was degraded by the crude enzyme, and two fractions were separately observed by gel permeation chromatography of the reaction products. One fr...

journal_title：Applied and environmental microbiology

authors： Tsuchii A,Takeda K

更新日期：1990-01-01 00:00:00

abstract：:Lactobacillus gasseri LA39 and L. reuteri LA6 isolated from feces of the same human infant were found to produce similar cyclic bacteriocins (named gassericin A and reutericin 6, respectively) that cannot be distinguished by molecular weights or primary amino acid sequences. However, reutericin 6 has a narrower spectr...

journal_title：Applied and environmental microbiology

authors： Kawai Y,Ishii Y,Arakawa K,Uemura K,Saitoh B,Nishimura J,Kitazawa H,Yamazaki Y,Tateno Y,Itoh T,Saito T

更新日期：2004-05-01 00:00:00

abstract：:Sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses revealed multiple alkB gene copies/cell in soil bacterial isolates and an apparently high genetic mobility among various phylogenetic groups. Identifying alkane degraders by alkB terminal restriction fragments (T-RFs) and sequences is str...

journal_title：Applied and environmental microbiology

authors： Giebler J,Wick LY,Schloter M,Harms H,Chatzinotas A

更新日期：2013-05-01 00:00:00

abstract：:White-nose syndrome is a fungal disease that has decimated bat populations across eastern North America. Identification of the etiologic agent, Pseudogymnoascus destructans (formerly Geomyces destructans), in environmental samples is essential to proposed management plans. A major challenge is the presence of closely ...

journal_title：Applied and environmental microbiology

authors： Shuey MM,Drees KP,Lindner DL,Keim P,Foster JT

更新日期：2014-03-01 00:00:00

abstract：:Thirty-eight cultures of Legionella pneumophila isolated from surface waters were characterized by their morphological, tinctorial, biochemical, and serological properties and by their ability to produce disease in guinea pigs. Their susceptibility to antimicrobial agents also was tested. When they were compared with...

journal_title：Applied and environmental microbiology

authors： Orrison LH,Cherry WB,Fliermans CB,Dees SB,McDougal LK,Dodd DJ

更新日期：1981-07-01 00:00:00

abstract：:Whole cells of the parent strain of Beijerinckia, grown with succinate and biphenyl, oxidized dibenzo-p-dioxin and several chlorinated dioxins. The rate of oxidation of the chlorinated dibenzo-p-dioxins decreased with an increasing degree of chlorine substitution. A mutant strain (B8/36) of Beijerinckia oxidized diben...

journal_title：Applied and environmental microbiology

authors： Klecka GM,Gibson DT

更新日期：1980-02-01 00:00:00

abstract：:Mesophilic and psychrotrophic populations from refrigerated meat were identified in this study, and the spoilage potential of microbial isolates in packaged beef was evaluated by analyzing the release of volatile organic compounds (VOC) by gas chromatography-mass spectrometry (GC/MS). Fifty mesophilic and twenty-nine ...

journal_title：Applied and environmental microbiology

authors： Ercolini D,Russo F,Nasi A,Ferranti P,Villani F

更新日期：2009-04-01 00:00:00

abstract：:Sixty-three strains of lactic streptococci isolated from commercial lactic streptococcal starter cultures were examined for lysogeny by treatment with ultraviolet light or mitomycin C. After treatment with the inducing agent, all strains, whether or not they lysed, were examined for evidence of phage release by electr...

journal_title：Applied and environmental microbiology

authors： Huggins AR,Sandine WE

更新日期：1977-01-01 00:00:00

abstract：:Salmonella typhimurium accumulates glutamate in response to osmotic stress. Cells in aerobic exponential growth have an intracellular pool of approximately 125 nmol of glutamate mg of protein-1. When cells were grown in minimal medium with 500 mM NaCl, KCl, or sucrose, 290 to 430 nmol of glutamate was found to accumul...

journal_title：Applied and environmental microbiology

authors： Botsford JL,Alvarez M,Hernandez R,Nichols R

更新日期：1994-07-01 00:00:00

abstract：:We investigated the genetic diversity of symbiotic bacteria associated with two newly discovered species of Osedax from Monterey Canyon, CA, at 1,017-m (Osedax Monterey Bay sp. 3 "rosy" [Osedax sp. MB3]) and 381-m (Osedax Monterey Bay sp. 4 "yellow collar") depths. Quantitative PCR and clone libraries of 16S rRNA gene...

journal_title：Applied and environmental microbiology

authors： Goffredi SK,Johnson SB,Vrijenhoek RC

更新日期：2007-04-01 00:00:00

abstract：:Batch cultures of a lithotrophic Fe(II)-oxidizing bacterium, strain BrT, isolated from the rhizosphere of a wetland plant, were grown in bioreactors and used to determine the significance of microbial Fe(II) oxidation at circumneutral pH and to identify abiotic variables that affect the partitioning between microbial ...

journal_title：Applied and environmental microbiology

authors： Neubauer SC,Emerson D,Megonigal JP

更新日期：2002-08-01 00:00:00