Global regulation of the response to sulfur availability in the cheese-related bacterium Brevibacterium aurantiacum.

Abstract:

:In this study, we combined metabolic reconstruction, growth assays, and metabolome and transcriptome analyses to obtain a global view of the sulfur metabolic network and of the response to sulfur availability in Brevibacterium aurantiacum. In agreement with the growth of B. aurantiacum in the presence of sulfate and cystine, the metabolic reconstruction showed the presence of a sulfate assimilation pathway, thiolation pathways that produce cysteine (cysE and cysK) or homocysteine (metX and metY) from sulfide, at least one gene of the transsulfuration pathway (aecD), and genes encoding three MetE-type methionine synthases. We also compared the expression profiles of B. aurantiacum ATCC 9175 during sulfur starvation or in the presence of sulfate. Under sulfur starvation, 690 genes, including 21 genes involved in sulfur metabolism and 29 genes encoding amino acids and peptide transporters, were differentially expressed. We also investigated changes in pools of sulfur-containing metabolites and in expression profiles after growth in the presence of sulfate, cystine, or methionine plus cystine. The expression of genes involved in sulfate assimilation and cysteine synthesis was repressed in the presence of cystine, whereas the expression of metX, metY, metE1, metE2, and BL613, encoding a probable cystathionine-γ-synthase, decreased in the presence of methionine. We identified three ABC transporters: two operons encoding transporters were transcribed more strongly during cysteine limitation, and one was transcribed more strongly during methionine depletion. Finally, the expression of genes encoding a methionine γ-lyase (BL929) and a methionine transporter (metPS) was induced in the presence of methionine in conjunction with a significant increase in volatile sulfur compound production.

journal_name

Appl Environ Microbiol

authors

Forquin MP,Hébert A,Roux A,Aubert J,Proux C,Heilier JF,Landaud S,Junot C,Bonnarme P,Martin-Verstraete I

doi

10.1128/AEM.01708-10

subject

Has Abstract

pub_date

2011-02-01 00:00:00

pages

1449-59

issue

4

eissn

0099-2240

issn

1098-5336

pii

AEM.01708-10

journal_volume

77

pub_type

杂志文章
  • Altered protein expression of Streptococcus oralis cultured at low pH revealed by two-dimensional gel electrophoresis.

    abstract::Streptococcus oralis is the predominant aciduric nonmutans streptococcus isolated from the human dentition, but the role of this organism in the initiation and progression of dental caries has yet to be established. To identify proteins that are differentially expressed by S. oralis growing under conditions of low pH,...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.8.3396-3405.2001

    authors: Wilkins JC,Homer KA,Beighton D

    更新日期:2001-08-01 00:00:00

  • Detection of low levels of Listeria monocytogenes cells by using a fiber-optic immunosensor.

    abstract::Biosensor technology has a great potential to meet the need for sensitive and nearly real-time microbial detection from foods. An antibody-based fiber-optic biosensor to detect low levels of Listeria monocytogenes cells following an enrichment step was developed. The principle of the sensor is a sandwich immunoassay w...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.10.6138-6146.2004

    authors: Geng T,Morgan MT,Bhunia AK

    更新日期:2004-10-01 00:00:00

  • Sorption and metabolism of metolachlor by a bacterial community.

    abstract::A stable bacterial community absorbed and transformed the herbicide metolachlor [2-chloro-N-(2-ethyl- 6-methylphenyl)-N-(2-methoxy-1-methylethyl)-acetamide] from a liquid medium. About 80% of the added ring-[U-C]metolachlor (50 mug/ml) disappeared from the medium and accumulated inside the cells. The ratio of cellular...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.3.733-740.1989

    authors: Liu SY,Zheng Z,Zhang R,Bollag JM

    更新日期:1989-03-01 00:00:00

  • Inhibition of predation by Bdellovibrio bacteriovorus and Micavibrio aeruginosavorus via host cell metabolic activity in the presence of carbohydrates.

    abstract::Bdellovibrio bacteriovorus and Micavibrio aeruginosavorus are highly motile Gram-negative predatory bacteria with the potential of being used as biocontrol agents or living antibiotics. It was suggested previously that sugar-binding proteins play a role in M. aeruginosavorus and B. bacteriovorus host specificity and p...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02565-10

    authors: Dashiff A,Keeling TG,Kadouri DE

    更新日期:2011-04-01 00:00:00

  • Physiological characterization of the ARO10-dependent, broad-substrate-specificity 2-oxo acid decarboxylase activity of Saccharomyces cerevisiae.

    abstract::Aerobic, glucose-limited chemostat cultures of Saccharomyces cerevisiae CEN.PK113-7D were grown with different nitrogen sources. Cultures grown with phenylalanine, leucine, or methionine as a nitrogen source contained high levels of the corresponding fusel alcohols and organic acids, indicating activity of the Ehrlich...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.6.3276-3284.2005

    authors: Vuralhan Z,Luttik MA,Tai SL,Boer VM,Morais MA,Schipper D,Almering MJ,Kötter P,Dickinson JR,Daran JM,Pronk JT

    更新日期:2005-06-01 00:00:00

  • Role of endoproteolytic dibasic proprotein processing in maturation of secretory proteins in Trichoderma reesei.

    abstract::Cell extracts of Trichoderma reesei exhibited dibasic endopeptidase activity toward the carboxylic side of KR, RR, and PR sequences. This activity was stimulated by the presence of Ca2+ ions and localized in vesicles of low bouyant density; it therefore exhibited some similarity to yeast Kex2. Analytical chromatofocus...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.9.3202-3208.1998

    authors: Goller SP,Schoisswohl D,Baron M,Parriche M,Kubicek CP

    更新日期:1998-09-01 00:00:00

  • Clostridium cellulolyticum Viability and Sporulation under Cellobiose Starvation Conditions.

    abstract::Depending on the moment of cellobiose starvation, Clostridium cellulolyticum cells behave in different ways. Cells starved during the exponential phase of growth sporulate at 30%, whereas exhaustion of the carbon substrate at the beginning of growth does not provoke cell sporulation. Growth in the presence of excess c...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.3.868-871.1995

    authors: Gehin A,Gelhaye E,Raval G,Petitdemange H

    更新日期:1995-03-01 00:00:00

  • Characterization of lytic enzyme open reading frame 9 (ORF9) derived from Enterococcus faecalis bacteriophage phiEF24C.

    abstract::In bacteriophage (phage) therapy against Gram-positive bacteria, such as Staphylococcus aureus, Listeria monocytogenes, and Enterococcus faecalis, members of a genus of SPO1-like viruses are typically employed because of their extreme virulence and broad host spectrum. Phage φEF24C, which is a SPO1-like virus infectin...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01540-10

    authors: Uchiyama J,Takemura I,Hayashi I,Matsuzaki S,Satoh M,Ujihara T,Murakami M,Imajoh M,Sugai M,Daibata M

    更新日期:2011-01-01 00:00:00

  • First evidence of lysogeny in Propionibacterium freudenreichii subsp. shermanii.

    abstract::Dairy propionic acid bacteria, particularly the species Propionibacterium freudenreichii, play a major role in the ripening of Swiss type cheese. Isometric and filamentous bacteriophages infecting P. freudenreichii have previously been isolated from cheese. In order to determine the origin of these bacteriophages, lys...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.1.231-238.2001

    authors: Hervé C,Coste A,Rouault A,Fraslin JM,Gautier M

    更新日期:2001-01-01 00:00:00

  • Nanometer-scale visualization and structural analysis of the inorganic/organic hybrid structure of Gallionella ferruginea twisted stalks.

    abstract::The so-called Fe/Mn-oxidizing bacteria have long been recognized for their potential to form extracellular iron hydroxide or manganese oxide structures in aquatic environments. Bacterial species belonging to the genus Gallionella, one type of such bacteria, oxidize iron and produce uniquely twisted extracellular stalk...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02867-10

    authors: Suzuki T,Hashimoto H,Matsumoto N,Furutani M,Kunoh H,Takada J

    更新日期:2011-05-01 00:00:00

  • Carbon conversion efficiency and limits of productive bacterial degradation of methyl tert-butyl ether and related compounds.

    abstract::The utilization of the fuel oxygenate methyl tert-butyl ether (MTBE) and related compounds by microorganisms was investigated in a mainly theoretical study based on the Y(ATP) concept. Experiments were conducted to derive realistic maintenance coefficients and K(s) values needed to calculate substrate fluxes available...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01899-06

    authors: Müller RH,Rohwerder T,Harms H

    更新日期:2007-03-01 00:00:00

  • Coexistence of bacterial sulfide oxidizers, sulfate reducers, and spirochetes in a gutless worm (Oligochaeta) from the Peru margin.

    abstract::Olavius crassitunicatus is a small symbiont-bearing worm that occurs at high abundance in oxygen-deficient sediments in the East Pacific Ocean. Using comparative 16S rRNA sequence analysis and fluorescence in situ hybridization, we examined the diversity and phylogeny of bacterial symbionts in two geographically dista...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.3.1553-1561.2005

    authors: Blazejak A,Erséus C,Amann R,Dubilier N

    更新日期:2005-03-01 00:00:00

  • Field-based stable isotope probing reveals the identities of benzoic acid-metabolizing microorganisms and their in situ growth in agricultural soil.

    abstract::We used a combination of stable isotope probing (SIP), gas chromatography-mass spectrometry-based respiration, isolation/cultivation, and quantitative PCR procedures to discover the identity and in situ growth of soil microorganisms that metabolize benzoic acid. We added [(13)C]benzoic acid or [(12)C]benzoic acid (100...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00464-08

    authors: Pumphrey GM,Madsen EL

    更新日期:2008-07-01 00:00:00

  • Effect of raw-milk cheese consumption on the enterococcal flora of human feces.

    abstract::Enterococci are one of the major facultative anaerobic bacterial groups that reside in the human gastrointestinal tract. In the present study, the composition of the enterococcal fecal flora in three healthy humans was analyzed before, during, and after the daily consumption of approximately 125 g of a raw-milk Chedda...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.1.312-319.2003

    authors: Gelsomino R,Vancanneyt M,Cogan TM,Swings J

    更新日期:2003-01-01 00:00:00

  • Multivariate logistic regression for predicting total culturable virus presence at the intake of a potable-water treatment plant: novel application of the atypical coliform/total coliform ratio.

    abstract::Predicting the presence of enteric viruses in surface waters is a complex modeling problem. Multiple water quality parameters that indicate the presence of human fecal material, the load of fecal material, and the amount of time fecal material has been in the environment are needed. This paper presents the results of ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02780-06

    authors: Black LE,Brion GM,Freitas SJ

    更新日期:2007-06-01 00:00:00

  • Propagation of Rhizopus javanicus Biosorbent.

    abstract::After propagation of Rhizopus javanicus in defined media containing glucose, urea, and mineral salts in deionized distilled water, the ability of the nonliving biomass to sequester cupric ion was assayed. Growth, uptake capacity (saturation uptake at >1 mM Cu concentration in solution), and biosorptive yield (biomass ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.48.1.137-141.1984

    authors: Treen-Sears ME,Martin SM,Volesky B

    更新日期:1984-07-01 00:00:00

  • Phylogenetic characterization of 16S rRNA gene clones from deep-groundwater microorganisms that pass through 0.2-micrometer-pore-size filters.

    abstract::A total of 247 clones of 16S rRNA genes from microorganisms captured by 0.2- and 0.1-microm-pore-size filters from sedimentary and granite rock aquifers were amplified and yielded 37 operational taxonomic units (OTUs). Fifteen OTUs captured by 0.1-microm-pore-size filters were affiliated with the candidate divisions O...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.2.1084-1088.2005

    authors: Miyoshi T,Iwatsuki T,Naganuma T

    更新日期:2005-02-01 00:00:00

  • Genome Evolution and Nitrogen Fixation in Bacterial Ectosymbionts of a Protist Inhabiting Wood-Feeding Cockroaches.

    abstract:UNLABELLED:By combining genomics and isotope imaging analysis using high-resolution secondary ion mass spectrometry (NanoSIMS), we examined the function and evolution of Bacteroidales ectosymbionts of the protist Barbulanympha from the hindguts of the wood-eating cockroach Cryptocercus punctulatus In particular, we inv...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00611-16

    authors: Tai V,Carpenter KJ,Weber PK,Nalepa CA,Perlman SJ,Keeling PJ

    更新日期:2016-07-15 00:00:00

  • Identification of Enterococcus spp. with a biochemical key.

    abstract::A six-step biochemical key is presented for the identification of all recognized Enterococcus spp. The key consists of 12 tests, but no more than 6 are needed for the most complicated identification. The reliability of the key has been evaluated with collection type strains and clinical and environmental isolates. Thi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.10.4425-4430.1999

    authors: Manero A,Blanch AR

    更新日期:1999-10-01 00:00:00

  • Mediation Analysis as a Means of Identifying Dietary Components That Differentially Affect the Fecal Microbiota of Infants Weaned by Modified Baby-Led and Traditional Approaches.

    abstract::The introduction of "solids" (i.e., complementary foods) to the milk-only diet in early infancy affects the development of the gut microbiota. The aim of this study was to determine whether a "baby-led" approach to complementary feeding that encourages the early introduction of an adult-type diet results in alteration...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1128/AEM.00914-18

    authors: Leong C,Haszard JJ,Lawley B,Otal A,Taylor RW,Szymlek-Gay EA,Fleming EA,Daniels L,Fangupo LJ,Tannock GW,Heath AM

    更新日期:2018-08-31 00:00:00

  • Pathways involved in reductant distribution during photobiological H(2) production by Rhodobacter sphaeroides.

    abstract::We used global transcript analyses and mutant studies to investigate the pathways that impact H(2) production in the photosynthetic bacterium Rhodobacter sphaeroides. We found that H(2) production capacity is related to the levels of expression of the nitrogenase and hydrogenase enzymes and the enzymes of the Calvin-B...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.05273-11

    authors: Kontur WS,Ziegelhoffer EC,Spero MA,Imam S,Noguera DR,Donohue TJ

    更新日期:2011-10-01 00:00:00

  • Genome-driven investigation of compatible solute biosynthesis pathways of Pseudomonas syringae pv. syringae and their contribution to water stress tolerance.

    abstract::The foliar pathogen Pseudomonas syringae pv. syringae exhibits an exceptional ability to survive on asymptomatic plants as an epiphyte. Intermittent wetting events on plants lead to osmotic and matric stresses which must be tolerated for survival as an epiphyte. In this study, we have applied bioinformatic, genetic, a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00686-10

    authors: Kurz M,Burch AY,Seip B,Lindow SE,Gross H

    更新日期:2010-08-01 00:00:00

  • Composition and structure of microbial communities from stromatolites of Hamelin Pool in Shark Bay, Western Australia.

    abstract::Stromatolites, organosedimentary structures formed by microbial activity, are found throughout the geological record and are important markers of biological history. More conspicuous in the past, stromatolites occur today in a few shallow marine environments, including Hamelin Pool in Shark Bay, Western Australia. Ham...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.8.4822-4832.2005

    authors: Papineau D,Walker JJ,Mojzsis SJ,Pace NR

    更新日期:2005-08-01 00:00:00

  • Listeria monocytogenes {sigma}B has a small core regulon and a conserved role in virulence but makes differential contributions to stress tolerance across a diverse collection of strains.

    abstract::Listeria monocytogenes strains are classified in at least three distinct phylogenetic lineages. There are correlations between lineage classification and source of bacterial isolation; e.g., human clinical and food isolates usually are classified in either lineage I or II. However, human clinical isolates are overrepr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00031-10

    authors: Oliver HF,Orsi RH,Wiedmann M,Boor KJ

    更新日期:2010-07-01 00:00:00

  • RNA arbitrarily primed PCR and fourier transform infrared spectroscopy reveal plasticity in the acid tolerance response of Streptococcus macedonicus.

    abstract::We have previously reported that an acid tolerance response (ATR) can be induced in Streptococcus macedonicus cells at mid-log phase after autoacidification, transient exposure to acidic pH, or acid habituation, as well as at stationary phase. Here, we compared the transcriptional profiles of these epigenetic phenotyp...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00315-08

    authors: Papadimitriou K,Boutou E,Zoumpopoulou G,Tarantilis PA,Polissiou M,Vorgias CE,Tsakalidou E

    更新日期:2008-10-01 00:00:00

  • Sediment microbial community structure and mercury methylation in mercury-polluted Clear Lake, California.

    abstract::Spatial and temporal variations in sediment microbial community structure in a eutrophic lake polluted with inorganic mercury were identified using polar lipid fatty acid (PLFA) analysis. Microbial community structure was strongly related to mercury methylation potential, sediment organic carbon content, and lake loca...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.4.1479-1488.2000

    authors: Macalady JL,Mack EE,Nelson DC,Scow KM

    更新日期:2000-04-01 00:00:00

  • Construction of a lactococcal expression vector: expression of hen egg white lysozyme in Lactococcus lactis subsp. lactis.

    abstract::A pair of vectors for expression of heterologous genes in Lactococcus lactis was constructed. In addition to an origin of replication that has a broad host range, these vectors contain a multiple cloning site flanked by gene expression signals originating from L. lactis subsp. cremoris Wg2. The two vectors, about 3.7 ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.1.224-228.1989

    authors: van de Guchte M,van der Vossen JM,Kok J,Venema G

    更新日期:1989-01-01 00:00:00

  • Identification of disulfides from the biodegradation of dibenzothiophene.

    abstract::Several investigations have identified benzothiophene-2,3-dione in the organic solvent extracts of acidified cultures degrading dibenzothiophene via the Kodama pathway. In solution at neutral pH, the 2,3-dione exists as 2-mercaptophenylglyoxylate, which cyclizes upon acidification and is extracted as the 2,3-dione. Th...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.11.5084-5093.2001

    authors: Bressler DC,Fedorak PM

    更新日期:2001-11-01 00:00:00

  • BTI1, an azoreductase with pH-dependent substrate specificity.

    abstract::The group II azoreductase BTI1 utilizes NADPH to directly cleave azo bonds in water-soluble azo dyes, including quenchers of fluorescence. Unexpectedly, optimal reduction was dye specific, ranging from a pH of <5.5 for Janus green B, to pH 6.0 for methyl red, methyl orange, and BHQ-10, to pH >8.3 for flame orange. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02289-10

    authors: Johansson HE,Johansson MK,Wong AC,Armstrong ES,Peterson EJ,Grant RE,Roy MA,Reddington MV,Cook RM

    更新日期:2011-06-01 00:00:00

  • Preservation of Rhizobium viability and symbiotic infectivity by suspension in water.

    abstract::Three Rhizobium japonicum strains and two slow-growing cowpea-type Rhizobium strains were found to remain viable and able to rapidly modulate their respective hosts after being stored in purified water at ambient temperatures for periods of 1 year and longer. Three fast-growing Rhizobium species did not remain viable ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.47.5.895-900.1984

    authors: Crist DK,Wyza RE,Mills KK,Bauer WD,Evans WR

    更新日期:1984-05-01 00:00:00