Abstract:
:Three Rhizobium japonicum strains and two slow-growing cowpea-type Rhizobium strains were found to remain viable and able to rapidly modulate their respective hosts after being stored in purified water at ambient temperatures for periods of 1 year and longer. Three fast-growing Rhizobium species did not remain viable under the same water storage conditions. After dilution of slow-growing Rhizobium strains with water to 10(3) to 10(5) cells ml-1, the bacteria multiplied until the viable cell count reached levels of between 10(6) and 10(7) cells ml-1. The viable cell count subsequently remained fairly constant. When the rhizobia were diluted to 10(7) cells ml-1, they did not multiply, but full viability was maintained. If the rhizobia were washed and suspended at 10(9) cells ml-1, viability slowly declined to 10(7) cells ml-1 during 9 months of storage. Scanning electron microscopy showed that no major morphological changes took place during storage. Preservation of slow-growing rhizobia in water suspensions could provide a simple and inexpensive alternative to current methods for the preservation of rhizobia for legume inoculation.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Crist DK,Wyza RE,Mills KK,Bauer WD,Evans WRdoi
10.1128/AEM.47.5.895-900.1984subject
Has Abstractpub_date
1984-05-01 00:00:00pages
895-900issue
5eissn
0099-2240issn
1098-5336journal_volume
47pub_type
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pub_type: 杂志文章
doi:10.1128/AEM.56.4.956-962.1990
更新日期:1990-04-01 00:00:00
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journal_title:Applied and environmental microbiology
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2003-07-01 00:00:00