Examination of the seasonal dynamics of the toxic dinoflagellate Alexandrium catenella at Redondo Beach, California, by quantitative PCR.

abstract：:The strictly anaerobic pathogenic bacterium Clostridium difficile occurs in the human gut and is able to thrive from fermentation of leucine. Thereby the amino acid is both oxidized to isovalerate plus CO(2) and reduced to isocaproate. In the reductive branch of this pathway, the dehydration of (R)-2-hydroxyisocaproyl...

journal_title：Applied and environmental microbiology

authors： Kim J,Darley D,Selmer T,Buckel W

更新日期：2006-09-01 00:00:00

abstract：:Extraction of high-quality DNA is a key step in PCR detection of Cryptosporidium and other pathogens in environmental samples. Currently, Cryptosporidium oocysts in water samples have to be purified from water concentrates before DNA is extracted. This study compared the effectiveness of six DNA extraction methods (DN...

journal_title：Applied and environmental microbiology

authors： Jiang J,Alderisio KA,Singh A,Xiao L

更新日期：2005-03-01 00:00:00

abstract：:A real-time PCR-based method targeting the 18S rRNA gene was developed for the quantitative detection of Hartmannella vermiformis, a free-living amoeba which is a potential host for Legionella pneumophila in warm water systems and cooling towers. The detection specificity was validated using genomic DNA of the closely...

journal_title：Applied and environmental microbiology

authors： Kuiper MW,Valster RM,Wullings BA,Boonstra H,Smidt H,van der Kooij D

更新日期：2006-09-01 00:00:00

abstract：:Carnobacterium maltaromaticum UAL307, isolated from fresh pork, exhibits potent activity against a number of gram-positive organisms, including numerous Listeria species. Three bacteriocins were isolated from culture supernatant, and using matrix-assisted laser desorption ionization-time of flight mass spectrometry an...

journal_title：Applied and environmental microbiology

authors： Martin-Visscher LA,van Belkum MJ,Garneau-Tsodikova S,Whittal RM,Zheng J,McMullen LM,Vederas JC

更新日期：2008-08-01 00:00:00

abstract：:Marine bacteria play a central role in the degradation of dimethylsulfoniopropionate (DMSP) to dimethyl sulfide (DMS) and acrylic acid, DMS being critical to cloud formation and thereby cooling effects on the climate. High concentrations of DMSP and DMS have been reported in scleractinian coral tissues although, to da...

journal_title：Applied and environmental microbiology

authors： Raina JB,Tapiolas D,Willis BL,Bourne DG

更新日期：2009-06-01 00:00:00

abstract：:Increased listerial barotolerance at elevated osmolarity is attributed, in part, to the presence of accumulated betaine and L-carnitine. The percentage of listerial survival following exposure to 400 MPa for 5 min increased from 0.008 to 0.02% with added L-carnitine (5 mM) and to 0.05% with added betaine (5 mM). Furth...

journal_title：Applied and environmental microbiology

authors： Smiddy M,Sleator RD,Patterson MF,Hill C,Kelly AL

更新日期：2004-12-01 00:00:00

abstract：:Cytochrome c(3) from Desulfovibrio vulgaris Miyazaki F was successfully expressed in the facultative aerobe Shewanella oneidensis MR-1 under anaerobic, microaerophilic, and aerobic conditions, with yields of 0.3 to 0.5 mg of cytochrome/g of cells. A derivative of the broad-host-range plasmid pRK415 containing the cyto...

journal_title：Applied and environmental microbiology

authors： Ozawa K,Tsapin AI,Nealson KH,Cusanovich MA,Akutsu H

更新日期：2000-09-01 00:00:00

abstract：:A new virulent phage belonging to the Siphoviridae family and able to infect Lactococcus garvieae strains was isolated from compost soil. Phage GE1 has a prolate capsid (56 by 38 nm) and a long noncontractile tail (123 nm). It had a burst size of 139 and a latent period of 31 min. Its host range was limited to only tw...

journal_title：Applied and environmental microbiology

authors： Eraclio G,Tremblay DM,Lacelle-Côté A,Labrie SJ,Fortina MG,Moineau S

更新日期：2015-12-01 00:00:00

abstract：:The potential of Pseudomonas pseudoflava to produce poly-beta-hydroxyalkanoates (PHAs) from pentoses was studied. This organism was able to use a hydrolysate from the hemicellulosic fraction of poplar wood as a carbon and energy source for its growth. However, in batch cultures, growth was inhibited completely at hydr...

journal_title：Applied and environmental microbiology

authors： Bertrand JL,Ramsay BA,Ramsay JA,Chavarie C

更新日期：1990-10-01 00:00:00

abstract：:The wastewater of livestock slaughterhouses is considered a source of antimicrobial-resistant bacteria with clinical relevance and may thus be important for their dissemination into the environment. To get an overview of their occurrence and characteristics, we investigated process water (n = 50) from delivery and unc...

journal_title：Applied and environmental microbiology

authors： Savin M,Bierbaum G,Hammerl JA,Heinemann C,Parcina M,Sib E,Voigt A,Kreyenschmidt J

更新日期：2020-04-01 00:00:00

abstract：:A modified mariner transposon, miniHimar RB1, was generated to mutagenize cells of the metal-reducing bacterium Shewanella oneidensis. The use of this transposon led to the isolation of stable mutants and allowed rapid identification of disrupted genes. Fifty-eight mutants, including BG104 and BG148 with transposon in...

journal_title：Applied and environmental microbiology

authors： Bouhenni R,Gehrke A,Saffarini D

更新日期：2005-08-01 00:00:00

abstract：:The cytopathology for rat neuroblastoma cells (B-103) and the pathogenicity for B6C3F1 mice of four species of Naegleria have been compared. Both live amoebae and cell-free extracts of N. australiensis, N. fowleri, N. gruberi, and N. lovaniensis added to 51Cr-labeled B-103 cells caused release of radiolabel. All four ...

journal_title：Applied and environmental microbiology

authors： Marciano-Cabral FM,Fulford DE

更新日期：1986-05-01 00:00:00

abstract：:With the submersible JAGO and by scuba diving we discovered three remarkable geothermal cones, rising 33, 25, and 45 m from the seafloor at a depth of 65 m in Eyjafjordur, northern Iceland. The greatest geothermal activity was on the highest cone, which discharged up to 50 liters of freshwater per s at 72 degrees C an...

journal_title：Applied and environmental microbiology

authors： Marteinsson VT,Kristjánsson JK,Kristmannsdóttir H,Dahlkvist M,Saemundsson K,Hannington M,Pétursdóttir SK,Geptner A,Stoffers P

更新日期：2001-02-01 00:00:00

abstract：:A quantitative real-time PCR targeting the tnaA gene was studied to detect Escherichia coli and distinguish E. coli from Shigella spp. These microorganisms revealed high similarity in the molecular organization of the tna operon. ...

journal_title：Applied and environmental microbiology

authors： Bernasconi C,Volponi G,Picozzi C,Foschino R

更新日期：2007-10-01 00:00:00

abstract：:Giardia muris cysts were separated from fecal contaminants in primary isolates by unit gravity velocity sedimentation. Crude isolates obtained by centrifugation over 1.0 M sucrose were overlaid onto a Percoll density gradient, 1.01 to 1.03 g/ml. G. muris cysts were well separated from faster-sedimenting fecal debris a...

journal_title：Applied and environmental microbiology

authors： Sauch JF

更新日期：1984-08-01 00:00:00

abstract：:The ability of Escherichia coli MV1184 to accumulate inorganic phosphate (Pi) was enhanced by manipulating the genes involved in the transport and metabolism of Pi. The high-level Pi accumulation was achieved by modifying the genetic regulation and increasing the dosage of the E. coli genes encoding polyphosphate kina...

journal_title：Applied and environmental microbiology

authors： Kato J,Yamada K,Muramatsu A,Hardoyo,Ohtake H

更新日期：1993-11-01 00:00:00

abstract：:Many people in Japan often detect an unpleasant odor generated from laundry that is hung to dry indoors or when using their already-dried laundry. Such an odor is often described as a "wet-and-dirty-dustcloth-like malodor" or an "acidic or sweaty odor." In this study, we isolated the major microorganisms associated wi...

journal_title：Applied and environmental microbiology

authors： Kubota H,Mitani A,Niwano Y,Takeuchi K,Tanaka A,Yamaguchi N,Kawamura Y,Hitomi J

更新日期：2012-05-01 00:00:00

abstract：:3-Deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase (EC 2.5.1.54) catalyzes the first step of the shikimate pathway that finally leads to the biosynthesis of aromatic amino acids phenylalanine (Phe), tryptophan (Trp), and tyrosine (Tyr). In Corynebacterium glutamicum ATCC 13032, two chromosomal genes, NCgl0950...

journal_title：Applied and environmental microbiology

authors： Liu YJ,Li PP,Zhao KX,Wang BJ,Jiang CY,Drake HL,Liu SJ

更新日期：2008-09-01 00:00:00

abstract：:Bacterial utilization of crude oil components, such as the n-alkanes, requires complex cell surface adaptation to allow adherence to oil. To better understand microbial cell surface adaptation to growth on crude oil, the cell surface characteristics of two Pseudomonas aeruginosa strains, U1 and U3, both isolated from ...

journal_title：Applied and environmental microbiology

authors： Norman RS,Frontera-Suau R,Morris PJ

更新日期：2002-10-01 00:00:00

abstract：:The microaerophilic bacterium Campylobacter jejuni is the most common cause of bacterial food-borne infections in the developed world. Tolerance to environmental stress relies on proteases and chaperones in the cell envelope, such as HtrA and SurA. HtrA displays both chaperone and protease activities, but little is kn...

journal_title：Applied and environmental microbiology

authors： Baek KT,Vegge CS,Skórko-Glonek J,Brøndsted L

更新日期：2011-01-01 00:00:00

abstract：:In Europe, ixodid ticks are important arthropod vectors of human and animal pathogens, but comprehensive studies of the prevalence of all relevant pathogens in Central Europe are scarce. As a result of ecological changes, the incidences of tick-borne infections are expected to increase. In this study, 1,394 nymphal an...

journal_title：Applied and environmental microbiology

authors： Reye AL,Hübschen JM,Sausy A,Muller CP

更新日期：2010-05-01 00:00:00

abstract：:Populations of feral pigs (Sus scrofa) may serve as an environmental reservoir of Cryptosporidium parvum oocysts and Giardia sp. cysts for source water. We conducted a cross-sectional study to determine the prevalence of and associated demographic and environmental risk factors for the shedding of C. parvum oocysts an...

journal_title：Applied and environmental microbiology

authors： Atwill ER,Sweitzer RA,Pereira MG,Gardner IA,Van Vuren D,Boyce WM

更新日期：1997-10-01 00:00:00

abstract：:Enzymes with high specific activities at low temperatures have potential uses for chemical conversions when low temperatures are required, as in the food industry. Psychrotrophic microorganisms which grow at low temperatures may be a valuable source of cold-active enzymes that have higher activities at low temperature...

journal_title：Applied and environmental microbiology

authors： Trimbur DE,Gutshall KR,Prema P,Brenchley JE

更新日期：1994-12-01 00:00:00

abstract：:Rapid population growth and urban development along waterways and coastal areas have led to decreasing water quality. To examine the effects of upstream anthropogenic activities on microbiological water quality, methods for source-specific testing are required. In this study, molecular assays targeting human enterovir...

journal_title：Applied and environmental microbiology

authors： Fong TT,Griffin DW,Lipp EK

更新日期：2005-04-01 00:00:00

abstract：:Decisions as to the disposition of swine carcasses with lesions attributable to a mycobacterial infection are based upon a lesion criterion which is used as a diagnostic test by federal meat inspectors. Using this criterion, a federal meat inspector classified 58 of 100 pigs as "passed for cooking" and the other 42 pi...

journal_title：Applied and environmental microbiology

authors： Brown J,Neuman MA

更新日期：1979-04-01 00:00:00

abstract：:The effect of condensed tannins from birdsfoot trefoil (Lotus corniculatus L.) on the cellulolytic rumen bacterium Fibrobacter succinogenes S85 was examined. Condensed tannins inhibited endoglucanase activity in the extracellular culture fluid, at concentrations as low as 25 mug ml. In contrast, cell-associated endogl...

journal_title：Applied and environmental microbiology

authors： Bae HD,McAllister TA,Yanke J,Cheng KJ,Muir AD

更新日期：1993-07-01 00:00:00

abstract：:To prevent transmission of mycobacterial pathogens, medical devices must be disinfected by germicides with proven mycobactericidal activity. The quantitative carrier test EN 14563 provides an international standard for evaluation of the mycobactericidal activity of disinfectants under practical conditions. However, te...

journal_title：Applied and environmental microbiology

authors： Steinhauer K,Eschenbacher I,Radischat N,Detsch C,Niederweis M,Goroncy-Bermes P

更新日期：2010-01-01 00:00:00

abstract：:Two acetyl esterases (EC 3.1.1.6) were purified to gel electrophoretic homogeneity from Thermoanaerobacterium sp. strain JW/SL-YS485, an anaerobic, thermophilic endospore former which is able to utilize various substituted xylans for growth. Both enzymes released acetic acid from chemically acetylated larch xylan. Ace...

journal_title：Applied and environmental microbiology

authors： Shao W,Wiegel J

更新日期：1995-02-01 00:00:00

abstract：:Consumption of poultry contaminated with Campylobacter jejuni is a risk factor for human gastrointestinal disease. The rational development of control strategies for Campylobacter within chickens requires an understanding of the colonization process at the molecular and population levels, both within and between hosts...

journal_title：Applied and environmental microbiology

authors： Coward C,van Diemen PM,Conlan AJ,Gog JR,Stevens MP,Jones MA,Maskell DJ

更新日期：2008-06-01 00:00:00

abstract：:Many cyanobacterial strains are able to grow at a pH range from neutral to pH 10 or 11. Such alkaline conditions favor cyanobacterial growth (e.g., bloom formation), and cyanobacteria must have developed strategies to adjust to changes in CO2 concentration and ion availability. Synechocystis sp. strain PCC 6803 exhibi...

journal_title：Applied and environmental microbiology

authors： Summerfield TC,Sherman LA

更新日期：2008-09-01 00:00:00