Rapid evaluation of the mycobactericidal efficacy of disinfectants in the quantitative carrier test EN 14563 by using fluorescent Mycobacterium terrae.

Abstract:

:To prevent transmission of mycobacterial pathogens, medical devices must be disinfected by germicides with proven mycobactericidal activity. The quantitative carrier test EN 14563 provides an international standard for evaluation of the mycobactericidal activity of disinfectants under practical conditions. However, tests according to the EN 14563 standard are based on cultivation, and results are available only after 21 days. The aim of this study was to accelerate assessment of dosage and contact times of mycobactericidal preparations based on the EN 14563 standard. To this end, a gfp gene was constructed with a codon usage adapted for Mycobacterium tuberculosis. Expression of the gfp(m)(2+) gene in Mycobacterium terrae improved the detection sensitivity by 10-fold over that with a previously used reporter strain. Peracetic acid and a cation-active formulation were tested as commercially available disinfectants for medical devices. M. terrae expressing gfp(m)(2+) was used to determine dosage and contact times for the two test germicides. Fluorescence measurements correlated well with growth of the reporter strain, demonstrating that the fluorescence reliably indicated the number of viable cells. The fluorescence enabled us to determine the mycobactericidal efficacy of the test disinfectants according to the quantitative carrier test EN 14563 standard within at least 15 days. In conclusion, this study establishes gfp(m)(2+)-expressing M. terrae as a new reporter strain for reliable evaluation of mycobactericidal activities of disinfectants with a superior sensitivity and in a significantly shorter time than previously possible.

journal_name

Appl Environ Microbiol

authors

Steinhauer K,Eschenbacher I,Radischat N,Detsch C,Niederweis M,Goroncy-Bermes P

doi

10.1128/AEM.01660-09

subject

Has Abstract

pub_date

2010-01-01 00:00:00

pages

546-54

issue

2

eissn

0099-2240

issn

1098-5336

pii

AEM.01660-09

journal_volume

76

pub_type

杂志文章
  • In situ monitoring of the nascent Pseudomonas fluorescens biofilm response to variations in the dissolved organic carbon level in low-nutrient water by attenuated total reflectance-Fourier transform infrared spectroscopy.

    abstract::Drinking water quality management requires early warning tools which enable water supply companies to detect quickly and to forecast degradation of the microbial quality of drinking water during its transport throughout distribution systems. This study evaluated the feasibility of assessing, in real time, drinking wat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00838-07

    authors: Delille A,Quilès F,Humbert F

    更新日期:2007-09-01 00:00:00

  • Purification and Characterization of an l-Aminopeptidase from Pseudomonas putida ATCC 12633.

    abstract::An l-aminopeptidase of Pseudomonas putida, used in an industrial process for the hydrolysis of d,l-amino acid amide racemates, was purified to homogeneity. The highly l-enantioselective enzyme resembled thiol reagent-sensitive alkaline serine proteinases and was strongly activated by divalent cations. It possessed a h...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.12.4330-4334.1993

    authors: Hermes HF,Sonke T,Peters PJ,van Balken JA,Kamphuis J,Dijkhuizen L,Meijer EM

    更新日期:1993-12-01 00:00:00

  • Fed-batch approach to production of 2,3-butanediol by Klebsiella pneumoniae grown on high substrate concentrations.

    abstract::The bioconversion of sugars present in wood hemicellulose to 2,3-butanediol by Klebsiella pneumoniae grown on high sugar concentrations was investigated. When K. pneumoniae was grown under finite air conditions in the presence of added acetic acid, 50 g of D-glucose and D-xylose per liter could be converted to 25 and ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.46.3.630-635.1983

    authors: Yu EK,Saddler JN

    更新日期:1983-09-01 00:00:00

  • Fluorogenic selective and differential medium for isolation of fecal streptococci.

    abstract::Of 44 fluorogenic substrates tested for their ability to differentiate species of fecal streptococci, four yielded species-differentiating reactions. The remaining substrates either yielded uniformly positive, negative, or variable strain-dependent reactions. One substrate, 4-methylumbelliferone-alpha-D-galactoside, w...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.45.2.622-627.1983

    authors: Littel KJ,Hartman PA

    更新日期:1983-02-01 00:00:00

  • Commercially distributed meat as a potential vehicle for community-acquired methicillin-resistant Staphylococcus aureus.

    abstract::The incidence of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) infection has been increasing; however, the sources of infection remain unclear. Therefore, we investigated the involvement of meat as a possible mediator of CA-MRSA infection. We examined the distribution of MRSA strains in comm...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.07470-11

    authors: Ogata K,Narimatsu H,Suzuki M,Higuchi W,Yamamoto T,Taniguchi H

    更新日期:2012-04-01 00:00:00

  • Enhanced inactivation of Salmonella and Pseudomonas biofilms on stainless steel by use of T-128, a fresh-produce washing aid, in chlorinated wash solutions.

    abstract::The effect of the washing aid T-128 (generally recognized as safe [GRAS] formulation, composed mainly of phosphoric acid and propylene glycol) on inactivation of Salmonella and Pseudomonas populations in biofilms on stainless steel was evaluated under conditions of increasing organic matter loads in chlorinated wash s...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01094-12

    authors: Shen C,Luo Y,Nou X,Bauchan G,Zhou B,Wang Q,Millner P

    更新日期:2012-10-01 00:00:00

  • Diversity among Rhizobia Effective with Robinia pseudoacacia L.

    abstract::The diversity of rhizobia that form symbioses with roots of black locust (Robinia pseudoacacia L.), an economically important leguminous tree species, was examined by inoculating seedling root zones with samples of soil collected from the United States, Canada, and China. Bacteria were isolated from nodules, subcultur...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.7.2137-2143.1992

    authors: McCray Batzli J,Graves WR,van Berkum P

    更新日期:1992-07-01 00:00:00

  • Erg4A and Erg4B Are Required for Conidiation and Azole Resistance via Regulation of Ergosterol Biosynthesis in Aspergillus fumigatus.

    abstract::Ergosterol, a fungus-specific sterol enriched in cell plasma membranes, is an effective antifungal drug target. However, current knowledge of the ergosterol biosynthesis process in the saprophytic human fungal pathogen Aspergillus fumigatus remains limited. In this study, we found that two endoplasmic reticulum-locali...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02924-16

    authors: Long N,Xu X,Zeng Q,Sang H,Lu L

    更新日期:2017-02-01 00:00:00

  • Isolation and characterization of the gibberellin biosynthetic gene cluster in Sphaceloma manihoticola.

    abstract::Gibberellins (GAs) are tetracyclic diterpenoid phytohormones that were first identified as secondary metabolites of the fungus Fusarium fujikuroi (teleomorph, Gibberella fujikuroi). GAs were also found in the cassava pathogen Sphaceloma manihoticola, but the spectrum of GAs differed from that in F. fujikuroi. In contr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00694-08

    authors: Bömke C,Rojas MC,Gong F,Hedden P,Tudzynski B

    更新日期:2008-09-01 00:00:00

  • Survival of Vibrio cholerae and Escherichia coli in estuarine waters and sediments.

    abstract::In in vitro estuarine water and sediment chambers, the survival of Vibrio cholerae and Escherichia coli was determined by plate counting and direct counting techniques. V. cholerae strains included environmental, clinical, and serotype O1 and non-O1 isolates, whereas E. coli strains included ATCC 25922 and a freshly c...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.43.3.578-584.1982

    authors: Hood MA,Ness GE

    更新日期:1982-03-01 00:00:00

  • Automated systems for identification of heterotrophic marine bacteria on the basis of their Fatty Acid composition.

    abstract::The fatty acid methyl ester composition of a total of 71 marine strains representing the genera Alteromonas, Deleya, Oceanospirillum, and Vibrio was determined by gas-liquid chromatographic analysis. Over 70 different fatty acids were found. The predominant fatty acids were 16:0, 16:1 cis 9, summed-in-feature (SIF) 4 ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.6.2122-2132.1996

    authors: Bertone S,Giacomini M,Ruggiero C,Piccarolo C,Calegari L

    更新日期:1996-06-01 00:00:00

  • Plate diffusion assay as a rapid method for dosimetry of mutagens.

    abstract::This paper presents a method for determining mutagenic concentrations of chemicals by using an agar diffusion assay. The method is based on the linear relationship between the amount of chemical placed at the center of the dish and the radius of the mutagenic zone. A brief theoretical discussion and experimental data ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.38.6.1127-1131.1979

    authors: Awerbuch TE,Stark AA

    更新日期:1979-12-01 00:00:00

  • Genomic Variability of the Xanthomonas Pathovar mangiferaeindicae, Agent of Mango Bacterial Black Spot.

    abstract::The genetic diversity of 138 strains of the Xanthomonas pathovar mangiferaeindicae, which were isolated from three different hosts (mango, ambarella, and pepper tree) in 14 different countries, was assessed with restriction fragment length polymorphism markers. An analysis of patterns obtained by hybridization with an...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.1.246-253.1997

    authors: Gagnevin L,Leach JE,Pruvost O

    更新日期:1997-01-01 00:00:00

  • Conjugative transfer between Escherichia coli and Leptospira spp. as a new genetic tool.

    abstract::Our understanding of leptospiral pathogenesis, which remains poorly understood, depends on reliable genetic tools for functional analysis of genes in pathogenic strains. In this study, we report the first demonstration of conjugation between Escherichia coli and Leptospira spp. by using RP4 derivative conjugative plas...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02172-07

    authors: Picardeau M

    更新日期:2008-01-01 00:00:00

  • Complex physiology and compound stress responses during fermentation of alkali-pretreated corn stover hydrolysate by an Escherichia coli ethanologen.

    abstract::The physiology of ethanologenic Escherichia coli grown anaerobically in alkali-pretreated plant hydrolysates is complex and not well studied. To gain insight into how E. coli responds to such hydrolysates, we studied an E. coli K-12 ethanologen fermenting a hydrolysate prepared from corn stover pretreated by ammonia f...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.07329-11

    authors: Schwalbach MS,Keating DH,Tremaine M,Marner WD,Zhang Y,Bothfeld W,Higbee A,Grass JA,Cotten C,Reed JL,da Costa Sousa L,Jin M,Balan V,Ellinger J,Dale B,Kiley PJ,Landick R

    更新日期:2012-05-01 00:00:00

  • Immunoquantitative real-time PCR for detection and quantification of Staphylococcus aureus enterotoxin B in foods.

    abstract::A real-time immunoquantitative PCR (iqPCR) method for detection of Staphylococcus aureus enterotoxin B (SEB) was developed and evaluated using both pure cultures and foods. The assay consisted of immunocapture of SEB and real-time PCR amplification of the DNA probe linked to the detection antibody. iqPCR was compared ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03068-05

    authors: Rajkovic A,El-Moualij B,Uyttendaele M,Brolet P,Zorzi W,Heinen E,Foubert E,Debevere J

    更新日期:2006-10-01 00:00:00

  • Novel functions of (p)ppGpp and Cyclic di-GMP in mycobacterial physiology revealed by phenotype microarray analysis of wild-type and isogenic strains of Mycobacterium smegmatis.

    abstract::The bacterial second messengers (p)ppGpp and bis-(3'-5')-cyclic dimeric GMP (c-di-GMP) regulate important functions, such as transcription, virulence, biofilm formation, and quorum sensing. In mycobacteria, they regulate long-term survival during starvation, pathogenicity, and dormancy. Recently, a Pseudomonas aerugin...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03999-14

    authors: Gupta KR,Kasetty S,Chatterji D

    更新日期:2015-04-01 00:00:00

  • Utilization of ammonia nitrogen by intestinal bacteria isolated from pigs.

    abstract::In a medium containing ammonia, proteose peptone, and cysteine as nitrogen sources, 17 of 24 Bacteroidaceae strains, 3 of Selenomonas strains, 1 of 7 curved rods, 3 of 7 Spirochaetaceae strains, 8 of 20 Eubacterium strains, 8 of 13 Peptococcaceae strains, 3 of 4 Clostridium strains, 19 of 20 Enterobacteriaceae strains...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.39.1.30-35.1980

    authors: Takahashi M,Benno Y,Mitsuoka T

    更新日期:1980-01-01 00:00:00

  • Effect of beef broth protein on the thermal inactivation of staphylococcal enterotoxin B1.

    abstract::Enterotoxin B produced by Staphylococus aureus 243 in brain heart infusion broth was concentrated by dialysis against 40% polyethylene glycol (20 M), partially purified on a Sephadex G-100 column and heated at 110 degrees C in thermal death time cans. Various heating menstrua included 0.04 M Veronal buffer (pH 7.4), b...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.33.2.341-344.1977

    authors: Lee IC,Stevenson KE,Harmon LG

    更新日期:1977-02-01 00:00:00

  • Use of an Escherichia coli recombinant producing thermostable polyphosphate kinase as an ATP regenerator to produce fructose 1,6-diphosphate.

    abstract::Heat-treated Escherichia coli producing Thermus polyphosphate kinase regenerated ATP by using exogenous polyphosphate. This recombinant could be used as a platform to produce valuable compounds in combination with thermostable phosphorylating or energy-requiring enzymes. In this work, we demonstrated the production of...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00278-07

    authors: Iwamoto S,Motomura K,Shinoda Y,Urata M,Kato J,Takiguchi N,Ohtake H,Hirota R,Kuroda A

    更新日期:2007-09-01 00:00:00

  • Secretion of cryparin, a fungal hydrophobin.

    abstract::Cryparin is a cell-surface-associated hydrophobin of the filamentous ascomycete Cryphonectria parasitica. This protein contains a signal peptide that directs it to the vesicle-mediated secretory pathway. We detected a glycosylated form of cryparin in a secretory vesicle fraction, but secreted forms of this protein are...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.12.5431-5435.1999

    authors: McCabe PM,Van Alfen NK

    更新日期:1999-12-01 00:00:00

  • Diversity and antagonistic potential of bacteria associated with bryophytes from nutrient-poor habitats of the Baltic Sea Coast.

    abstract::Very little is known about the interaction of bryophytes with bacteria. Therefore, we analyzed bacteria associated with three bryophyte species, Tortula ruralis, Aulacomnium palustre, and Sphagnum rubellum, which represent typical moss species of three nutrient-poor plant communities at the southern Baltic Sea coast i...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.11.6569-6579.2004

    authors: Opelt K,Berg G

    更新日期:2004-11-01 00:00:00

  • Gut Microbial Divergence between Two Populations of the Hadal Amphipod Hirondellea gigas.

    abstract::Hadal environments sustain diverse microorganisms. A few studies have investigated hadal microbial communities consisting of free-living or particle-associated bacteria and archaea. However, animal-associated microbial communities in hadal environments remain largely unexplored, and comparative analyses of animal gut ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02032-18

    authors: Zhang W,Watanabe HK,Ding W,Lan Y,Tian RM,Sun J,Chen C,Cai L,Li Y,Oguri K,Toyofuku T,Kitazato H,Drazen JC,Bartlett D,Qian PY

    更新日期:2018-12-13 00:00:00

  • Thermal inactivation and injury of Bacillus stearothermophilus spores.

    abstract::Aqueous spore suspensions of Bacillus stearothermophilus ATCC 12980 were heated at different temperatures for various time intervals in a resistometer, spread plated on antibiotic assay medium supplemented with 0.1% soluble starch without (AAMS) or with (AAMS-S) 0.9% NaCl, and incubated at 55 degrees C unless otherwis...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.2.365-370.1987

    authors: Feeherry FE,Munsey DT,Rowley DB

    更新日期:1987-02-01 00:00:00

  • Pyrosequencing-based assessment of soil pH as a predictor of soil bacterial community structure at the continental scale.

    abstract::Soils harbor enormously diverse bacterial populations, and soil bacterial communities can vary greatly in composition across space. However, our understanding of the specific changes in soil bacterial community structure that occur across larger spatial scales is limited because most previous work has focused on eithe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00335-09

    authors: Lauber CL,Hamady M,Knight R,Fierer N

    更新日期:2009-08-01 00:00:00

  • Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the Gram-negative bacterium Escherichia coli.

    abstract::In this work we investigated the antibacterial properties of differently shaped silver nanoparticles against the gram-negative bacterium Escherichia coli, both in liquid systems and on agar plates. Energy-filtering transmission electron microscopy images revealed considerable changes in the cell membranes upon treatme...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02218-06

    authors: Pal S,Tak YK,Song JM

    更新日期:2007-03-01 00:00:00

  • Methodology for recovery of chemically treated Staphylococcus aureus with neutralizing medium.

    abstract::Recovery results of Staphylococcus aureus ATCC 6538 treated with phenolics and quaternary ammonium compounds on Dey and Engley (D/E) neutralizing medium at various time intervals were compared by the use of two commonly used media. Two recovery processes were utilized. In one, the chemically treated organisms were pla...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.45.5.1533-1537.1983

    authors: Dey BP,Engley FB Jr

    更新日期:1983-05-01 00:00:00

  • Identification, characterization, and molecular application of a virulence-associated autotransporter from a pathogenic Pseudomonas fluorescens strain.

    abstract::A gene, pfa1, encoding an autotransporter was cloned from a pathogenic Pseudomonas fluorescens strain, TSS, isolated from diseased fish. The expression of pfa1 is enhanced during infection and is regulated by growth phase and growth conditions. Mutation of pfa1 significantly attenuates the overall bacterial virulence ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00159-09

    authors: Hu YH,Liu CS,Hou JH,Sun L

    更新日期:2009-07-01 00:00:00

  • Gene Expression Analysis during Conidial Germ Tube and Appressorium Development in Colletotrichum trifolii.

    abstract::Preinfection development in Colletotrichum spp. exhibits three morphologies (conidia, germ tubes, and appressoria) and is directed by a complex interplay of environmental signals. Germ tube morphogenesis for Colletotrichum trifolii and the related fungus Colletotrichum gloeosporioides f. sp. aeschynomene was shown to ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.6.2378-2383.1997

    authors: Buhr TL,Dickman MB

    更新日期:1997-06-01 00:00:00

  • Diverse virulence gene content of Shiga toxin-producing Escherichia coli from finishing swine.

    abstract::Shiga toxin-producing Escherichia coli (STEC) infections are a critical public health concern because they can cause severe clinical outcomes, such as hemolytic uremic syndrome, in humans. Determining the presence or absence of virulence genes is essential in assessing the potential pathogenicity of STEC strains. Curr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01761-14

    authors: Tseng M,Fratamico PM,Bagi L,Delannoy S,Fach P,Manning SD,Funk JA

    更新日期:2014-10-01 00:00:00