Methodology for recovery of chemically treated Staphylococcus aureus with neutralizing medium.

abstract：:The aim of this work was to identify genes in Lactococcus lactis subsp. lactis IL1403 and Lactococcus lactis subsp. cremoris Wg2 important for adsorption of the 936-species phages bIL170 and phi 645, respectively. Random insertional mutagenesis of the two L. lactis strains was carried out with the vector pGh9:ISS1, an...

journal_title：Applied and environmental microbiology

authors： Dupont K,Janzen T,Vogensen FK,Josephsen J,Stuer-Lauridsen B

更新日期：2004-10-01 00:00:00

abstract：:The biotinylating reagent succinimidyl 6-(biotinamido)hexanoate was used to label the cell surfaces of the cosmopolitan, marine, eukaryotic microorganism Emiliania huxleyi under different growth conditions. Proteins characteristic of different nutrient conditions could be identified. In particular, a nitrogen-regulate...

journal_title：Applied and environmental microbiology

authors： Palenik B,Koke JA

更新日期：1995-09-01 00:00:00

abstract：UNLABELLED:The mycosporine-like amino acids (MAAs) are a group of small molecules with a diverse ecological distribution among microorganisms. MAAs have a range of physiological functions, including protection against UV radiation, making them important from a biotechnological perspective. In the present study, we iden...

journal_title：Applied and environmental microbiology

authors： D'Agostino PM,Javalkote VS,Mazmouz R,Pickford R,Puranik PR,Neilan BA

更新日期：2016-09-16 00:00:00

abstract：:The lysogenic bacteriophage APSE infects "Candidatus Hamiltonella defensa," a facultative endosymbiont of aphids and other sap-feeding insects. This endosymbiont has established a beneficial association with aphids, increasing survivorship following attack by parasitoid wasps. Although APSE and "Ca. Hamiltonella defen...

journal_title：Applied and environmental microbiology

authors： Degnan PH,Moran NA

更新日期：2008-11-01 00:00:00

abstract：:The role of Escherichia coli as a pathogen has been the focus of considerable study, while much less is known about it as a commensal and how it adapts to and colonizes different environmental niches within the mammalian gut. In this study, we characterize Escherichia coli organisms (n = 146) isolated from different r...

journal_title：Applied and environmental microbiology

authors： Abraham S,Gordon DM,Chin J,Brouwers HJ,Njuguna P,Groves MD,Zhang R,Chapman TA

更新日期：2012-10-01 00:00:00

abstract：UNLABELLED:Microbes can produce molecular hydrogen (H2) via fermentation, dinitrogen fixation, or direct photolysis, yet the H2 dynamics in cyanobacterial communities has only been explored in a few natural systems and mostly in the laboratory. In this study, we investigated the diel in situ H2 dynamics in a hot spring...

journal_title：Applied and environmental microbiology

authors： Revsbech NP,Trampe E,Lichtenberg M,Ward DM,Kühl M

更新日期：2016-06-30 00:00:00

abstract：:Using a chemically defined medium without L-alanine, Lactobacillus johnsonii was demonstrated to be strictly auxotrophic for that amino acid. A comparative genetic analysis showed that all known genes involved in L-alanine biosynthesis are absent from the genome of L. johnsonii. This auxotrophy was complemented by het...

journal_title：Applied and environmental microbiology

authors： van der Kaaij H,Desiere F,Mollet B,Germond JE

更新日期：2004-03-01 00:00:00

abstract：:Dehalococcoides sp. strain BAV1 couples growth with the reductive dechlorination of vinyl chloride (VC) to ethene. Degenerate primers targeting conserved regions in reductive dehalogenase (RDase) genes were designed and used to PCR amplify putative RDase genes from strain BAV1. Seven unique RDase gene fragments were i...

journal_title：Applied and environmental microbiology

authors： Krajmalnik-Brown R,Hölscher T,Thomson IN,Saunders FM,Ritalahti KM,Löffler FE

更新日期：2004-10-01 00:00:00

abstract：:Protein S is a major spore coat protein of Myxococcus xanthus, consisting of two homologous domains, the N-terminal domain (NTD) and the C-terminal domain, both of which contain a Ca(2+)-binding site. Protein S tightly binds to myxospores in a Ca(2+)-dependent manner. Here, we constructed a novel expression vector, pC...

journal_title：Applied and environmental microbiology

authors： Kobayashi H,Yoshida T,Inouye M

更新日期：2009-08-01 00:00:00

abstract：:Pectinolytic enzymes play an important role in cocoa fermentation. In this study, we characterized three extracellular pectate lyases (Pels) produced by bacilli isolated from fermenting cocoa beans. These enzymes, named Pel-22, Pel-66, and Pel-90, were synthesized by Bacillus pumilus BS22, Bacillus subtilis BS66, and ...

journal_title：Applied and environmental microbiology

authors： Ouattara HG,Reverchon S,Niamke SL,Nasser W

更新日期：2010-08-01 00:00:00

abstract：:We assayed the tolerance to solvents of three toluene-degrading Pseudomonas putida strains and Pseudomonas mendocina KR1 in liquid and soil systems. P. putida DOT-T1 tolerated concentrations of heptane, propylbenzene, octanol, and toluene of at least 10% (vol/vol), while P. putida F1 and EEZ15 grew well in the presenc...

journal_title：Applied and environmental microbiology

authors： Huertas MJ,Duque E,Marqués S,Ramos JL

更新日期：1998-01-01 00:00:00

abstract：:Two activities involved in terminal pathway conversion of sterigmatocystin to aflatoxin B1 were isolated from an aflatoxin-nonproducing mutant of Aspergillus parasiticus (avn-1), and the time course of appearance of the activities in culture was determined. Subcellular fractionation of fungal mycelia resolved the two ...

journal_title：Applied and environmental microbiology

authors： Cleveland TE,Lax AR,Lee LS,Bhatnagar D

更新日期：1987-07-01 00:00:00

abstract：:Cellular fatty acids of 10 strains of lactic acid bacteria were analyzed. The purpose of this work was to find lactic acid bacteria with high lactobacillic acid contents. The bacteria studied were unable to synthesize oleic acid. Some strains did not synthesize lactobacillic acid, although all were able to form dihydr...

journal_title：Applied and environmental microbiology

authors： Johnsson T,Nikkila P,Toivonen L,Rosenqvist H,Laakso S

更新日期：1995-12-01 00:00:00

abstract：:Accurate enumeration of viruses within environmental samples is critical for investigations of the ecological role of viruses and viral infection within microbial communities. This report evaluates differences in viral and bacterial direct counts between estuarine sediment samples which were either immediately process...

journal_title：Applied and environmental microbiology

authors： Helton RR,Liu L,Wommack KE

更新日期：2006-07-01 00:00:00

abstract：:Digital dermatitis (DD) is an important cause of lameness in dairy cattle worldwide. It has now been reported in beef cattle and also sheep (contagious ovine digital dermatitis [CODD]). Three Treponema phylogroups are consistently isolated from lesions, Treponema medium-like, Treponema phagedenis-like, and Treponema p...

journal_title：Applied and environmental microbiology

authors： Sullivan LE,Carter SD,Duncan JS,Grove-White DH,Angell JW,Evans NJ

更新日期：2015-11-01 00:00:00

abstract：:High-throughput sequencing of the taxonomically informative 16S rRNA gene provides a powerful approach for exploring microbial diversity. Here we compare the performances of two common "benchtop" sequencing platforms, Illumina MiSeq and Ion Torrent Personal Genome Machine (PGM), for bacterial community profiling by 16...

journal_title：Applied and environmental microbiology

authors： Salipante SJ,Kawashima T,Rosenthal C,Hoogestraat DR,Cummings LA,Sengupta DJ,Harkins TT,Cookson BT,Hoffman NG

更新日期：2014-12-01 00:00:00

abstract：:Growing bacteria have a high concentration of ribosomes to ensure sufficient protein synthesis, which is necessary for genome replication and cellular division. To elucidate whether metabolic activity of soil microorganisms is coupled with growth, we investigated the relationship between rRNA and DNA synthesis in a so...

journal_title：Applied and environmental microbiology

authors： Papp K,Hungate BA,Schwartz E

更新日期：2018-04-02 00:00:00

abstract：:Although there have been several reports on the efficacy assessment of a Salmonella enterica serovar Enteritidis vaccine against intestinal and parenchymatous organ diseases of laying hens, no public health risk characterization of its long-term effect on eggs has been reported. In this study, we attempted to assess t...

journal_title：Applied and environmental microbiology

authors： Toyota-Hanatani Y,Ekawa T,Ohta H,Igimi S,Hara-Kudo Y,Sasai K,Baba E

更新日期：2009-02-01 00:00:00

abstract：:An epoxyalkane:coenzyme M (CoM) transferase (EaCoMT) enzyme was recently found to be active in the aerobic vinyl chloride (VC) and ethene assimilation pathways of Mycobacterium strain JS60. In the present study, EaCoMT activity and genes were investigated in 10 different mycobacteria isolated on VC or ethene from dive...

journal_title：Applied and environmental microbiology

authors： Coleman NV,Spain JC

更新日期：2003-10-01 00:00:00

abstract：:Aerobic and facultatively anaerobic bacteria isolated from false-positive, presumptive, total coliform, most-probable-number tests of Chesapeake Bay oyster, water, and sediment samples were characterized and then classified by numerical taxonomy. A total of 538 bacterial strains clustered into 17 phena, the predominan...

journal_title：Applied and environmental microbiology

authors： Hussong D,Damaré JM,Weiner RM,Colwell RR

更新日期：1981-01-01 00:00:00

abstract：:The production of various eremophilane-type sesquiterpenes by Penicillium roqueforti strains has allowed us to propose a biochemical pathway for PR toxin synthesis. A time-course study of P. roqueforti metabolite production by high-performance liquid chromatography was performed to check this hypothetical pathway. The...

journal_title：Applied and environmental microbiology

authors： Moreau S,Lablache-Combier A,Biguet J

更新日期：1980-04-01 00:00:00

abstract：:The nonlinear thermal inactivation of Aspergillus niger spores in phosphate-citrate buffer was studied. The thermal inactivation pattern of the spore consisted of a shoulder, an accelerated decline, and a tail at various constant temperatures around 60 degrees C. The pattern fitted a thermotolerant subpopulation model...

journal_title：Applied and environmental microbiology

authors： Fujikawa H,Itoh T

更新日期：1996-10-01 00:00:00

abstract：:Two types of mesophilic, methanogenic bacteria were isolated in pure culture from anaerobic freshwater and marine mud with 2-propanol as the hydrogen donor. The freshwater strain (SK) was a Methanospirillum species, the marine, salt-requiring strain (CV), which had irregular coccoid cells, resembled Methanogenium sp. ...

journal_title：Applied and environmental microbiology

authors： Widdel F

更新日期：1986-05-01 00:00:00

abstract：:Two strains of Penicillium digitatum and one strain of Penicillium italicum were exposed to various levels of sorbic acid and potassium sorbate, and the MICs were determined. Selected strains of the molds were then repeatedly exposed to subinhibitory levels of the compounds to determine whether increased tolerance mig...

journal_title：Applied and environmental microbiology

authors： Schroeder LL,Bullerman LB

更新日期：1985-10-01 00:00:00

abstract：:The ultrasonic determination of microbial population was carried out by using a new apparatus composed of two piezoelectric membranes. When the apparatus was immersed in a medium containing cells and an arbitrary voltage was charged on a piezoelectric membrane, an output voltage was obtained from the other membrane. T...

journal_title：Applied and environmental microbiology

authors： Ishimori Y,Karube I,Suzuki S

更新日期：1981-10-01 00:00:00

abstract：:A monoclonal antibody designated 302 beta that is specific for the beta subunit of the oxygenase component (ISPTOL) of toluene dioxygenase from Pseudomonas putida F1 was used to prepare an immunoaffinity column. ISPTOL in cell extracts of Escherichia coli JM109(pDTG611) bound to the column, and an enzyme-linked immuno...

journal_title：Applied and environmental microbiology

authors： Lynch NA,Jiang H,Gibson DT

更新日期：1996-06-01 00:00:00

abstract：:Transfer of plasmid-borne antibiotic resistance genes in Escherichia coli wild-type strains is possible by transformation under naturally occurring conditions in oligotrophic, aquatic environments containing physiologic concentrations of calcium. In contrast, transformation is suppressed in nitrogen-rich body fluids l...

journal_title：Applied and environmental microbiology

authors： Woegerbauer M,Jenni B,Thalhammer F,Graninger W,Burgmann H

更新日期：2002-01-01 00:00:00

abstract：:The plasmid-free strain Lactococcus lactis subsp. cremoris BC101 produced an extracellular proteinase physicochemically similar to the proteinase encoded by the plasmid-linked prtP gene of other lactococcal strains. The absence of detectable plasmids in strain BC101 indicated that the prtP proteinase gene may be chrom...

journal_title：Applied and environmental microbiology

authors： Nissen-Meyer J,Lillehaug D,Nes IF

更新日期：1992-02-01 00:00:00

abstract：:Resting cells of Micrococcus luteus have been shown to remove strontium (Sr) from dilute aqueous solutions of SrCl(2) at pH 7. Loadings of 25 mg of Sr per g of cell dry weight were achieved by cells exposed to a solution containing 50 ppm (mg/liter) of Sr. Sr binding occurred in the absence of nutrients and did not re...

journal_title：Applied and environmental microbiology

authors： Faison BD,Cancel CA,Lewis SN,Adler HI

更新日期：1990-12-01 00:00:00

abstract：:Human norovirus (NoV) is the leading cause of nonbacterial acute gastroenteritis epidemics worldwide. High-pressure processing (HPP) has been considered a promising nonthermal processing technology to inactivate food- and waterborne viral pathogens. Due to the lack of an effective cell culture method for human NoV, th...

journal_title：Applied and environmental microbiology

authors： Lou F,Huang P,Neetoo H,Gurtler JB,Niemira BA,Chen H,Jiang X,Li J

更新日期：2012-08-01 00:00:00