Utilization of ammonia nitrogen by intestinal bacteria isolated from pigs.

abstract：:An assay has been developed to detect bacterial glycosidases in colonies grown on the surface of agar plates. Advantages of this technique over previously described methods include elimination of the need for replica plating, better visualization of chromagenic reaction products, and a simple permeabilization step to ...

journal_title：Applied and environmental microbiology

authors： Paoni NF,Arroyo RL

更新日期：1984-01-01 00:00:00

abstract：:The steady-state effect of 2,5,2',5'-tetrachlorobiphenyl (TCBP) on the green alga Selenastrum capricornutum was investigated in a P-limited two-stage chemostat system. The partition coefficient of this polychlorinated biphenyl congener was 5.9 x 10 in steady-state cultures. At a cellular TCBP concentration of 12.2 x 1...

journal_title：Applied and environmental microbiology

authors： Rhee GY,Shane L,Denucci A

更新日期：1988-06-01 00:00:00

abstract：:Staphylococcus aureus is a gram-positive pathogen that causes a variety of diseases, including bovine mastitis, which has severe economic consequences. Standard antibiotic treatment results in selection of resistant strains, leading to a need for alternative treatments, such as bacteriophage therapy. Forty-nine S. aur...

journal_title：Applied and environmental microbiology

authors： Synnott AJ,Kuang Y,Kurimoto M,Yamamichi K,Iwano H,Tanji Y

更新日期：2009-07-01 00:00:00

abstract：:Evaluating different swabbing materials for spore recovery efficiency (RE) from steel surfaces, we recorded the maximum RE (71%) of 10(7) Bacillus subtilis spores with Tulips cotton buds, followed by Johnson's cotton buds and standard Hi-Media cotton, polyester, nylon, and foam (23%) swabs. Among cotton swabs, instant...

journal_title：Applied and environmental microbiology

authors： Thomas P,Mujawar MM,Upreti R,Sekhar AC

更新日期：2013-01-01 00:00:00

abstract：:Fifty-one isolates of Rhizobium leguminosarum biovar phaseoli from various geographic and ecological sources, largely in Mexico, were characterized by the electrophoretic mobilities of 15 metabolic enzymes, and 46 distinctive multilocus genotypes (electrophoretic types [ETs]) were distinguished on the basis of allele ...

journal_title：Applied and environmental microbiology

authors： Pinero D,Martinez E,Selander RK

更新日期：1988-11-01 00:00:00

abstract：:A soy sauce koji mold, Aspergillus flavus var. columnaris Raper and Fennel (ATCC 44310), was treated with UV irradiation to obtain mutant strains possessing high protease activities, high amylase activities, and light-colored conidia. Selected mutant strains were tested for toxicity, and some were found acutely toxic ...

journal_title：Applied and environmental microbiology

authors： Kalayanamitr A,Bhumiratana A,Flegel TW,Glinsukon T,Shinmyo A

更新日期：1987-08-01 00:00:00

abstract：:The group II azoreductase BTI1 utilizes NADPH to directly cleave azo bonds in water-soluble azo dyes, including quenchers of fluorescence. Unexpectedly, optimal reduction was dye specific, ranging from a pH of <5.5 for Janus green B, to pH 6.0 for methyl red, methyl orange, and BHQ-10, to pH >8.3 for flame orange. ...

journal_title：Applied and environmental microbiology

authors： Johansson HE,Johansson MK,Wong AC,Armstrong ES,Peterson EJ,Grant RE,Roy MA,Reddington MV,Cook RM

更新日期：2011-06-01 00:00:00

abstract：:A spontaneous rpsL mutant of Thermus thermophilus was isolated in a search for new selection markers for this organism. This new allele, named rpsL1, encodes a K47R/K57E double mutant S12 ribosomal protein that confers a streptomycin-dependent (SD) phenotype to T. thermophilus. Models built on the available three-dime...

journal_title：Applied and environmental microbiology

authors： Blas-Galindo E,Cava F,López-Viñas E,Mendieta J,Berenguer J

更新日期：2007-08-01 00:00:00

abstract：:Shuttle vectors that replicate stably and express selectable phenotypes in both Thermococcus kodakaraensis and Escherichia coli have been constructed. Plasmid pTN1 from Thermococcus nautilis was ligated to the commercial vector pCR2.1-TOPO, and selectable markers were added so that T. kodakaraensis transformants could...

journal_title：Applied and environmental microbiology

authors： Santangelo TJ,Cubonová L,Reeve JN

更新日期：2008-05-01 00:00:00

abstract：:A method has been described which can compare the efficacy of different nongermicidal handwashing protocols for removal of transient microbial flora without the necessity of establishing or relying on a previously determined baseline for an individual subject. The wash effluent is collected, and colony counts for the ...

journal_title：Applied and environmental microbiology

authors： Vesley D,Lillquist DR,Le CT

更新日期：1985-05-01 00:00:00

abstract：:The cellulosomes produced by Clostridium cellulovorans are organized by the specific interactions between the cohesins in the scaffolding proteins and the dockerins of the catalytic components. Using a cohesin biomarker, we identified a cellulosomal enzyme which belongs to the glycosyl hydrolase family 5 and has a dom...

journal_title：Applied and environmental microbiology

authors： Jeon SD,Lee JE,Kim SJ,Park SH,Choi GW,Han SO

更新日期：2013-10-01 00:00:00

abstract：UNLABELLED:Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) was detected in 2003 and rapidly became the predominant MRSA clade in the Netherlands. Studies have shown that transmissions are difficult to identify, since this MRSA variant represents a genetically homogenous clade when current typ...

journal_title：Applied and environmental microbiology

authors： Bosch T,Witteveen S,Haenen A,Landman F,Schouls LM

更新日期：2016-06-30 00:00:00

abstract：:Cytophaga hutchinsonii is a widely distributed cellulolytic bacterium in the phylum Bacteroidetes. It can digest crystalline cellulose rapidly without free cellulases or cellulosomes. The mechanism of its cellulose utilization remains a mystery. We developed an efficient method based on a linear DNA double-crossover a...

journal_title：Applied and environmental microbiology

authors： Wang Y,Wang Z,Cao J,Guan Z,Lu X

更新日期：2014-10-01 00:00:00

abstract：:Trichoderma species isolated from water-damaged buildings were screened for toxicity by using boar sperm cells as indicator cells. The crude methanolic cell extract from Trichoderma harzianum strain ES39 inhibited the boar sperm cell motility at a low exposure concentration (50% effective concentration, 1 to 5 microg ...

journal_title：Applied and environmental microbiology

authors： Peltola J,Ritieni A,Mikkola R,Grigoriev PA,Pócsfalvi G,Andersson MA,Salkinoja-Salonen MS

更新日期：2004-08-01 00:00:00

abstract：:Streptococcus agalactiae (group B Streptococcus [GBS]) is a cause of severe infections, particularly during the newborn period. While methods exist for generating chromosomal mutations in GBS, they are cumbersome and inefficient and present significant challenges if the goal is to study subtle mutations, such as singl...

journal_title：Applied and environmental microbiology

authors： Hooven TA,Bonakdar M,Chamby AB,Ratner AJ

更新日期：2019-03-22 00:00:00

abstract：:Free-living amoebae are frequent hosts for bacterial endosymbionts. In this study, the symbionts of eight novel environmental Acanthamoeba strains isolated from different locations worldwide were characterized. Phylogenetic analysis revealed that they were related to one of four evolutionary lineages of amoeba symbion...

journal_title：Applied and environmental microbiology

authors： Schmitz-Esser S,Toenshoff ER,Haider S,Heinz E,Hoenninger VM,Wagner M,Horn M

更新日期：2008-09-01 00:00:00

abstract：:Mixed infections have important consequences for the ecology and evolution of host-parasite interactions. In vector-borne diseases, interactions between pathogens occur in both the vertebrate host and the arthropod vector. Spirochete bacteria belonging to the Borrelia burgdorferi sensu lato genospecies complex are tra...

journal_title：Applied and environmental microbiology

authors： Herrmann C,Gern L,Voordouw MJ

更新日期：2013-12-01 00:00:00

abstract：:Butane-oxidizing Arthrobacter (ATCC 27778) bacteria were shown to degrade low concentrations of methyl t-butyl ether (MTBE; range, 100 to 800 microg/liter) with an apparent half-saturation concentration (K(s)) of 2.14 mg/liter and a maximum substrate utilization rate (k(c)) of 0.43 mg/mg of total suspended solids per ...

journal_title：Applied and environmental microbiology

authors： Liu CY,Speitel GE Jr,Georgiou G

更新日期：2001-05-01 00:00:00

abstract：:Eubacterium lentum is a gram-positive, nonsporeforming, nonmotile, asaccharolytic anaerobe. In the present investigations, 3 E. lentum strains (group E) isolated from rat feces were compared with 30 E. lentum strains (groups A, B, C, and D) previously studied by Macdonald et al. (I. A. Macdonald, J. F. Jellet, D. E. M...

journal_title：Applied and environmental microbiology

authors： Eyssen H,Verhulst A

更新日期：1984-01-01 00:00:00

abstract：:Gas chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy were used to study the metabolism of deuterated n-alkanes (C6 to C12) and 1-13C-labeled n-hexane by a highly enriched sulfate-reducing bacterial culture. All substrates were activated via fumarate addition to form the corresponding alkyls...

journal_title：Applied and environmental microbiology

authors： Davidova IA,Gieg LM,Nanny M,Kropp KG,Suflita JM

更新日期：2005-12-01 00:00:00

abstract：:We have developed a microtiter plate method for screening a large number of bacterial isolates for the ability to grow on different crystalline polycyclic aromatic hydrocarbons (PAHs). Growth on PAHs cannot easily be determined with standard growth assays because of the very low aqueous solubility and bioavailability ...

journal_title：Applied and environmental microbiology

authors： Johnsen AR,Bendixen K,Karlson U

更新日期：2002-06-01 00:00:00

abstract：:To enhance food safety and stability, the food industry tends to use natural antimicrobials such as plant-derived compounds as an attractive alternative to chemical preservatives. Nonetheless, caution must be exercised in light of the potential for bacterial adaptation to these molecules, a phenomenon previously obser...

journal_title：Applied and environmental microbiology

authors： Dubois-Brissonnet F,Naïtali M,Mafu AA,Briandet R

更新日期：2011-02-01 00:00:00

abstract：:Ammonia-oxidizing bacteria (AOB) are thought to contribute significantly to N2O production and methane oxidation in soils. Most of our knowledge derives from experiments with Nitrosomonas europaea, which appears to be of minor importance in most soils compared to Nitrosospira spp. We have conducted a comparative study...

journal_title：Applied and environmental microbiology

authors： Jiang QQ,Bakken LR

更新日期：1999-06-01 00:00:00

abstract：:Seven organotin compounds and tin chloride were tested for their effects on the methanogenic bacteria Methanococcus thermolithotrophicus, Methanococcus deltae delta LH, and Methanosarcina barkeri 227. The methanogens were strongly inhibited by triethyltin, tripropyltin, and monophenyltin compounds, generally at concen...

journal_title：Applied and environmental microbiology

authors： Boopathy R,Daniels L

更新日期：1991-04-01 00:00:00

abstract：:Increased listerial barotolerance at elevated osmolarity is attributed, in part, to the presence of accumulated betaine and L-carnitine. The percentage of listerial survival following exposure to 400 MPa for 5 min increased from 0.008 to 0.02% with added L-carnitine (5 mM) and to 0.05% with added betaine (5 mM). Furth...

journal_title：Applied and environmental microbiology

authors： Smiddy M,Sleator RD,Patterson MF,Hill C,Kelly AL

更新日期：2004-12-01 00:00:00

abstract：:Several investigations have identified benzothiophene-2,3-dione in the organic solvent extracts of acidified cultures degrading dibenzothiophene via the Kodama pathway. In solution at neutral pH, the 2,3-dione exists as 2-mercaptophenylglyoxylate, which cyclizes upon acidification and is extracted as the 2,3-dione. Th...

journal_title：Applied and environmental microbiology

authors： Bressler DC,Fedorak PM

更新日期：2001-11-01 00:00:00

abstract：:The morphology and cellulases of Ruminococcus albus 8 were markedly affected by the inclusion of 3-phenylpropanoic acid (PPA) in a defined growth medium. PPA-grown bacteria produced substantial quantities of cell-bound cellulase, as well as a very high-molecular-weight extracellular enzyme and lesser amounts of two lo...

journal_title：Applied and environmental microbiology

authors： Stack RJ,Hungate RE

更新日期：1984-07-01 00:00:00

abstract：:Pseudomonas putida F1 and Pseudomonas sp. strain JS150 initiate toluene degradation by incorporating molecular oxygen into the aromatic nucleus to form cis-1,2-dihydroxy-3-methylcyclohexa-3,5-diene. When toluene-grown cells were incubated with 2- and 3-nitrotoluene, the major products identified were 2- and 3-nitroben...

journal_title：Applied and environmental microbiology

authors： Robertson JB,Spain JC,Haddock JD,Gibson DT

更新日期：1992-08-01 00:00:00

abstract：:Lipophilic compounds of the culture suspension containing Rhodococcus erythropolis DSM43215 had surfactant properties when the bacteria were cultivated with n-alkanes as the sole carbon source. Thirteen main components from a dichloromethane-methanol extract of the R. erythropolis cultures were isolated and characteri...

journal_title：Applied and environmental microbiology

authors： Kretschmer A,Bock H,Wagner F

更新日期：1982-10-01 00:00:00

abstract：:A variety of nitroaromatic compounds, including 2,4,6-trinitrotoluene (TNT), were reduced by hydrogen in the presence of enzyme preparations from Veillonella alkalescens. Consistent with the proposed reduction pathway, R-NO2 H2 leads to R-NO H2 leads to R-NHOH H2 leads to R-NH2, 3 mol of H2 was utilized per mol of nit...

journal_title：Applied and environmental microbiology

authors： McCormick NG,Feeherry FE,Levinson HS

更新日期：1976-06-01 00:00:00