Biotransformation of linoleic acid and bile acids by Eubacterium lentum.

Abstract:

:Eubacterium lentum is a gram-positive, nonsporeforming, nonmotile, asaccharolytic anaerobe. In the present investigations, 3 E. lentum strains (group E) isolated from rat feces were compared with 30 E. lentum strains (groups A, B, C, and D) previously studied by Macdonald et al. (I. A. Macdonald, J. F. Jellet, D. E. Mahony, and L. V. Holdeman, Appl. Environ. Microbiol. 37:992-1000, 1979). All strains alkalized (pH 8 to 8.5) arginine-containing (2 to 15 mg/ml) culture media, and growth of the majority of the strains was stimulated by arginine. All strains converted linoleic acid into transvaccenic acid by shifting the 12,13-cis double bond of linoleic acid into an 11,12-trans(?) double bond followed by biohydrogenation of the 9,10-cis double bond. Hence, biohydrogenation of linoleic acid is a new general characteristic of E. lentum. The 33 strains were also studied for bile acid deconjugase and hydroxysteroid dehydrogenase (HSDH) activities. The 6 strains in group D were steroid inactive; the 27 strains in groups A, B, C, and E were steroid active. The steroid-active group contained bile acid deconjugase-producing strains (groups C and E, plus strain 116 in group A) and nondeconjugating strains. All nondeconjugating strains of groups A and B developed 7 alpha- and 12 alpha-HSDH activities and contained 3 alpha-HSDH-positive strains and 3 alpha-HSDH-negative strains. Deconjugating strains varied in HSDH activities.

journal_name

Appl Environ Microbiol

authors

Eyssen H,Verhulst A

doi

10.1128/AEM.47.1.39-43.1984

subject

Has Abstract

pub_date

1984-01-01 00:00:00

pages

39-43

issue

1

eissn

0099-2240

issn

1098-5336

journal_volume

47

pub_type

杂志文章
  • Biodegradation of p-nitrophenol via 1,2,4-benzenetriol by an Arthrobacter sp.

    abstract::The degradation of p-nitrophenol (PNP) by Moraxella and Pseudomonas spp. involves an initial monooxygenase-catalyzed removal of the nitro group. The resultant hydroquinone is subject to ring fission catalyzed by a dioxygenase enzyme. We have isolated a strain of an Arthrobacter sp., JS443, capable of degrading PNP wit...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.8.3030-3032.1994

    authors: Jain RK,Dreisbach JH,Spain JC

    更新日期:1994-08-01 00:00:00

  • Enhancing nitrite inhibition of Clostridium botulinum with isoascorbate in perishable canned cured meat.

    abstract::Addition of sodium isoascorbate to the formulation for perishable canned comminuted cured meat markedly enhanced the efficacy of nitrite against Clostridium botulinum. This effect was reproducible through a series of three tests. In one test it was found that the initial addition of 50 microgram of sodium nitrite per ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.35.1.59-61.1978

    authors: Tompkin RB,Christiansen LN,Shaparis AB

    更新日期:1978-01-01 00:00:00

  • Oxidation of ethylene glycol by a salt-requiring bacterium.

    abstract::Bacterium T-52, cultured on ethylene glycol, readily oxidized glycolate and glyoxylate and exhibited elevated activities of ethylene glycol dehydrogenase and glycolate oxidase. Labeled glyoxylate was identified in reaction mixtures containing [C]-ethylene glycol, but no glycolate was detected. The most likely pathway ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.42.1.180-183.1981

    authors: Caskey WH,Taber WA

    更新日期:1981-07-01 00:00:00

  • Bradyrhizobium japonicum Survival in and Soybean Inoculation with Fluid Gels.

    abstract::The utilization of gels, which are used for fluid drilling of seeds, as carriers of Bradyrhizobium japonicum for soybean (Glycine max (L.) Merr.) inoculation was studied. Gels of various chemical composition (magnesium silicate, potassium acrylate-acrylamide, grafted starch, and hydroxyethyl cellulose) were used, alth...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.3.617-622.1989

    authors: Jawson MD,Franzluebbers AJ,Berg RK

    更新日期:1989-03-01 00:00:00

  • The motility symbiont of the termite gut flagellate Caduceia versatilis is a member of the "Synergistes" group.

    abstract::The flagellate Caduceia versatilis in the gut of the termite Cryptotermes cavifrons reportedly propels itself not by its own flagella but solely by the flagella of ectosymbiotic bacteria. Previous microscopic observations have revealed that the motility symbionts are flagellated rods partially embedded in the host cel...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00750-07

    authors: Hongoh Y,Sato T,Dolan MF,Noda S,Ui S,Kudo T,Ohkuma M

    更新日期:2007-10-01 00:00:00

  • Degradation of Pyridine by Micrococcus luteus Isolated from Soil.

    abstract::An organism capable of growth on pyridine was isolated from soil by enrichment culture techniques and identified as Micrococcus luteus. The organism oxidized pyridine for energy and released N contained in the pyridine ring as ammonium. The organism could not grow on mono- or disubstituted pyridinecarboxylic acids or ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.51.5.963-968.1986

    authors: Sims GK,Sommers LE,Konopka A

    更新日期:1986-05-01 00:00:00

  • Expression of a Clostridium thermocellum endoglucanase gene in Lactobacillus plantarum.

    abstract::Recombinant plasmid pM25 containing the celE gene of Clostridium thermocellum, which codes for an enzymatically active endoglucanase, was transformed into Lactobacillus plantarum by electroporation. Strains harboring pM25 expressed thermostable endoglucanase, which was found predominantly in the culture medium. Two ot...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.8.2095-2097.1989

    authors: Bates EE,Gilbert HJ,Hazlewood GP,Huckle J,Laurie JI,Mann SP

    更新日期:1989-08-01 00:00:00

  • Expanding the known diversity and environmental distribution of an uncultured phylogenetic division of bacteria.

    abstract::Culture-independent molecular phylogenetic methods were used to explore the breadth of diversity and environmental distribution of members of the division-level "candidate" phylogenetic group WS6, recently discovered in a contaminated aquifer and with no cultivated representatives. A broad diversity of WS6-affiliated ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.4.1617-1621.2000

    authors: Dojka MA,Harris JK,Pace NR

    更新日期:2000-04-01 00:00:00

  • A CRISPRi-dCas9 System for Archaea and Its Use To Examine Gene Function during Nitrogen Fixation by Methanosarcina acetivorans.

    abstract::CRISPR-based systems are emerging as the premier method to manipulate many cellular processes. In this study, a simple and efficient CRISPR interference (CRISPRi) system for targeted gene repression in archaea was developed. The Methanosarcina acetivorans CRISPR-Cas9 system was repurposed by replacing Cas9 with the ca...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01402-20

    authors: Dhamad AE,Lessner DJ

    更新日期:2020-10-15 00:00:00

  • Rubber-degrading enzyme from a bacterial culture.

    abstract::Rubber-degrading activity was found in the extracellular culture medium of Xanthomonas sp. strain 35Y which was grown on natural rubber latex. Natural rubber in the latex state was degraded by the crude enzyme, and two fractions were separately observed by gel permeation chromatography of the reaction products. One fr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.1.269-274.1990

    authors: Tsuchii A,Takeda K

    更新日期:1990-01-01 00:00:00

  • Relationships and Evolution of Double-Stranded RNA Totiviruses of Yeasts Inferred from Analysis of L-A-2 and L-BC Variants in Wine Yeast Strain Populations.

    abstract::Saccharomyces cerevisiae killer strains secrete a protein toxin active on nonkiller strains of the same (or other) yeast species. Different killer toxins, K1, K2, K28, and Klus, have been described. Each toxin is encoded by a medium-size (1.5- to 2.3-kb) M double-stranded RNA (dsRNA) located in the cytoplasm. M dsRNAs...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02991-16

    authors: Rodríguez-Cousiño N,Esteban R

    更新日期:2017-02-01 00:00:00

  • Inability of Pseudomonas stutzeri Denitrification Mutants with the Phenotype of Pseudomonas aeruginosa to Grow in Nitrous Oxide.

    abstract::[This corrects the article on p. 1301 in vol. 50.]. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.51.3.671-671.1986

    authors:

    更新日期:1986-03-01 00:00:00

  • Algicidal Activity of Streptomyces eurocidicus JXJ-0089 Metabolites and Their Effects on Microcystis Physiology.

    abstract:UNLABELLED:Copper sulfate (CuSO4) has been widely used as an algicide to control harmful cyanobacterial blooms (CyanoHABs) in freshwater lakes. However, there are increasing concerns about this application, due mainly to the general toxicity of CuSO4 to other aquatic species and its long-term persistence in the environ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01198-16

    authors: Zhang BH,Ding ZG,Li HQ,Mou XZ,Zhang YQ,Yang JY,Zhou EM,Li WJ

    更新日期:2016-08-15 00:00:00

  • Characterization of urease-positive thermophilic Campylobacter subspecies by multilocus enzyme electrophoresis typing.

    abstract::Thirty-one urease-positive thermophilic Campylobacter (UPTC) isolates, including three reference strains (NCTC12892, NCTC12895 and NCTC12896), and three Campylobacter lari isolates, which were isolated from several countries and sources, were compared genotypically by using multilocus enzyme electrophoresis (MLEE). We...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.6.3308-3310.2003

    authors: Matsuda M,Kaneko A,Stanley T,Millar BC,Miyajima M,Murphy PG,Moore JE

    更新日期:2003-06-01 00:00:00

  • Production of xanthomegnin and viomellein by species of Aspergillus correlated with mycotoxicosis produced in mice.

    abstract::By using thin-layer chromatography and infrared spectroscopy, xanthomegnin and viomellein have been isolated and identified from species of the Aspergillus ochraceus group. A correlation was established between the occurrence of these fungal quinones in the fungal cultural products and the ability of these products to...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.36.6.819-823.1978

    authors: Robbers JE,Hong S,Tuite J,Carlton WW

    更新日期:1978-12-01 00:00:00

  • Characterization of Fluorescent Siderophore-Mediated Iron Uptake in Pseudomonas sp. Strain M114: Evidence for the Existence of an Additional Ferric Siderophore Receptor.

    abstract::In Pseudomonas sp. strain M114, the outer membrane receptor for ferric pseudobactin M114 was shown to transport ferric pseudobactins B10 and A225, in addition to its own. The gene encoding this receptor, which was previously cloned on pCUP3, was localized by Tn5 mutagenesis to a region comprising >1.6 kb of M114 DNA. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.2.630-635.1992

    authors: Morris J,O'sullivan DJ,Koster M,Leong J,Weisbeek PJ,O'gara F

    更新日期:1992-02-01 00:00:00

  • Temperature and pH conditions that prevail during fermentation of sausages are optimal for production of the antilisterial bacteriocin sakacin K.

    abstract::Sakacin K is an antilisterial bacteriocin produced by Lactobacillus sake CTC 494, a strain isolated from Spanish dry fermented sausages. The biokinetics of cell growth and bacteriocin production of L. sake CTC 494 in vitro during laboratory fermentations were investigated by making use of MRS broth. The data obtained ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.3.974-981.1999

    authors: Leroy F,de Vuyst L

    更新日期:1999-03-01 00:00:00

  • Physiological characterization of viable-but-nonculturable Campylobacter jejuni cells.

    abstract::Campylobacter jejuni is a pathogenic, microaerophilic, gram-negative, mesophilic bacterium. Three strains isolated from humans with enteric campylobacteriosis were able to survive at high population levels (10(7) cells ml-1) as viable-but-nonculturable (VBNC) forms in microcosm water. The VBNC forms of the three C. je...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.3.1110-1116.1999

    authors: Tholozan JL,Cappelier JM,Tissier JP,Delattre G,Federighi M

    更新日期:1999-03-01 00:00:00

  • Significant enhanced expression and solubility of human proteins in Escherichia coli by fusion with protein S from Myxococcus xanthus.

    abstract::Protein S is a major spore coat protein of Myxococcus xanthus, consisting of two homologous domains, the N-terminal domain (NTD) and the C-terminal domain, both of which contain a Ca(2+)-binding site. Protein S tightly binds to myxospores in a Ca(2+)-dependent manner. Here, we constructed a novel expression vector, pC...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00691-09

    authors: Kobayashi H,Yoshida T,Inouye M

    更新日期:2009-08-01 00:00:00

  • Purification and characterization of two highly thermophilic alkaline lipases from Thermosyntropha lipolytica.

    abstract::Two thermostable lipases were isolated and characterized from Thermosyntropha lipolytica DSM 11003, an anaerobic, thermophilic, alkali-tolerant bacterium which grows syntrophically with methanogens on lipids such as olive oil, utilizing only the liberated fatty acid moieties but not the glycerol. Lipases LipA and LipB...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01509-07

    authors: Salameh MA,Wiegel J

    更新日期:2007-12-01 00:00:00

  • gerR, a novel ger operon involved in L-alanine- and inosine-initiated germination of Bacillus cereus ATCC 14579.

    abstract::Bacillus cereus endospores germinate in response to particular nutrients. Spores are able to sense these nutrients in the environment by receptors encoded by the gerA family of operons. Analysis of the Bacillus cereus ATCC 14579 genome revealed seven gerA family homologues. Using a transposon Tn917-based insertional m...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.2.774-781.2005

    authors: Hornstra LM,de Vries YP,de Vos WM,Abee T,Wells-Bennik MH

    更新日期:2005-02-01 00:00:00

  • Comparison of gull feces-specific assays targeting the 16S rRNA genes of Catellicoccus marimammalium and Streptococcus spp.

    abstract::Two novel gull-specific quantitative PCR (qPCR) assays were developed using 16S rRNA gene sequences from gull fecal clone libraries: a SYBR green assay targeting Streptococcus spp. (gull3) and a hydrolysis TaqMan assay targeting Catellicoccus marimammalium (gull4). The objectives of this study were to compare the host...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.07192-11

    authors: Ryu H,Griffith JF,Khan IU,Hill S,Edge TA,Toledo-Hernandez C,Gonzalez-Nieves J,Santo Domingo J

    更新日期:2012-03-01 00:00:00

  • Molecular assays for detecting Aphanomyces invadans in ulcerative mycotic fish lesions.

    abstract::The pathogenic oomycete Aphanomyces invadans is the primary etiological agent in ulcerative mycosis, an ulcerative skin disease caused by a fungus-like agent of wild and cultured fish. We developed sensitive PCR and fluorescent peptide nucleic acid in situ hybridization (FISH) assays to detect A. invadans. Laboratory-...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.72.2.1551-1557.2006

    authors: Vandersea MW,Litaker RW,Yonnish B,Sosa E,Landsberg JH,Pullinger C,Moon-Butzin P,Green J,Morris JA,Kator H,Noga EJ,Tester PA

    更新日期:2006-02-01 00:00:00

  • Development of an F57 sequence-based real-time PCR assay for detection of Mycobacterium avium subsp. paratuberculosis in milk.

    abstract::A light cycler-based real-time PCR (LC-PCR) assay that amplifies the F57 sequence of Mycobacterium avium subsp. paratuberculosis was developed. This assay also includes an internal amplification control template to monitor the amplification conditions in each reaction. The targeted F57 sequence element is unique for M...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.10.5957-5968.2005

    authors: Tasara T,Stephan R

    更新日期:2005-10-01 00:00:00

  • Isolation from sewage influent and characterization of novel Staphylococcus aureus bacteriophages with wide host ranges and potent lytic capabilities.

    abstract::Staphylococcus aureus is a gram-positive pathogen that causes a variety of diseases, including bovine mastitis, which has severe economic consequences. Standard antibiotic treatment results in selection of resistant strains, leading to a need for alternative treatments, such as bacteriophage therapy. Forty-nine S. aur...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02641-08

    authors: Synnott AJ,Kuang Y,Kurimoto M,Yamamichi K,Iwano H,Tanji Y

    更新日期:2009-07-01 00:00:00

  • Identification of bacteriophages for biocontrol of the kiwifruit canker phytopathogen Pseudomonas syringae pv. actinidiae.

    abstract::Pseudomonas syringae pv. actinidiae is a reemerging pathogen which causes bacterial canker of kiwifruit (Actinidia sp.). Since 2008, a global outbreak of P. syringae pv. actinidiae has occurred, and in 2010 this pathogen was detected in New Zealand. The economic impact and the development of resistance in P. syringae ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00062-14

    authors: Frampton RA,Taylor C,Holguín Moreno AV,Visnovsky SB,Petty NK,Pitman AR,Fineran PC

    更新日期:2014-04-01 00:00:00

  • Isolation of tellurite- and selenite-resistant bacteria from hydrothermal vents of the Juan de Fuca Ridge in the Pacific Ocean.

    abstract::Deep-ocean hydrothermal-vent environments are rich in heavy metals and metalloids and present excellent sites for the isolation of metal-resistant microorganisms. Both metalloid-oxide-resistant and metalloid-oxide-reducing bacteria were found. Tellurite- and selenite-reducing strains were isolated in high numbers from...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.9.4613-4622.2002

    authors: Rathgeber C,Yurkova N,Stackebrandt E,Beatty JT,Yurkov V

    更新日期:2002-09-01 00:00:00

  • Enzyme-linked immunosorbent assays for detection of xylem-limited bacteria: use of trinder reagent.

    abstract::Quantitation and detection of xylem-limited bacteria with an enzyme-linked immunosorbent assay with a peroxidase conjugate is described. The use of the Trinder reagent (4-aminoantipyrine) allows the determination of extremely small quantities of peroxidase with no precipitate formation or inactivation of the enzyme by...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.46.2.344-347.1983

    authors: Saunders MS,French WJ

    更新日期:1983-08-01 00:00:00

  • Differential expression of virulence and stress fitness genes between Escherichia coli O157:H7 strains with clinical or bovine-biased genotypes.

    abstract::Escherichia coli O157:H7 strains can be classified into different genotypes based on the presence of specific Shiga toxin-encoding bacteriophage insertion sites. Certain O157:H7 genotypes predominate among human clinical cases (clinical genotypes), while others are more frequently found in bovines (bovine-biased genot...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01666-09

    authors: Vanaja SK,Springman AC,Besser TE,Whittam TS,Manning SD

    更新日期:2010-01-01 00:00:00

  • Microbial aldicarb transformation in aquifer, lake, and salt marsh sediments.

    abstract::The microbial transformation of [N-methyl-(sup14)C]aldicarb, a carbamate pesticide, occurred in aquifer, lake, and salt marsh sediments. Microbial degradation of aldicarb took place within 21 days in aquifer sediments from sites previously exposed to aldicarb (Jamesport, Long Island, N.Y.) but did not occur in sedimen...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.8.2820-2829.1995

    authors: Kazumi J,Capone DG

    更新日期:1995-08-01 00:00:00