Enzyme-linked immunosorbent assays for detection of xylem-limited bacteria: use of trinder reagent.

Abstract:

:Quantitation and detection of xylem-limited bacteria with an enzyme-linked immunosorbent assay with a peroxidase conjugate is described. The use of the Trinder reagent (4-aminoantipyrine) allows the determination of extremely small quantities of peroxidase with no precipitate formation or inactivation of the enzyme by H(2)O(2). Comparison of the enzyme-linked immunosorbent assay method with microscopic and histochemical tests for the presence of the phony peach disease bacterium in 9-year-old "June Gold" peach trees gave comparable results. The peroxidase conjugate with the Trinder reagent is more sensitive than the alkaline phosphatase conjugate typically used for enzyme-linked immunosorbent assay quantitation.

journal_name

Appl Environ Microbiol

authors

Saunders MS,French WJ

doi

10.1128/AEM.46.2.344-347.1983

keywords:

subject

Has Abstract

pub_date

1983-08-01 00:00:00

pages

344-7

issue

2

eissn

0099-2240

issn

1098-5336

journal_volume

46

pub_type

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