Genetic organization of the mle locus and identification of a mleR-like gene from Leuconostoc oenos.

Abstract:

:Characterization of the mle locus harboring the malolactic enzyme gene mleA and malate permease gene mleP from Leuconostoc oenos was completed in this study by mRNA analysis. Northern (RNA) blot experiments revealed a 2.6-kb transcript, suggesting an operon structure harboring mleA and mleP genes. Primer extension analysis showed that the mle operon has a single transcription start site located 17 nucleotides upstream of the ATG translation start site for the mleA gene. We found sequences, TTGACT and TATGAT (which are separated by 18 bp), that are closely related to the gram-positive and Escherichia coli consensus promoter sequences. Upstream of the mleA gene, an 894-bp open reading frame that transcribed divergently from the mle operon was found. Sequence analysis and expression in E. coli minicells suggest that this open reading frame encodes a polypeptide with an apparent molecular mass of 34 kDa belonging to the LysR-type regulatory protein family. Protein comparisons showed the highest level of identity with the MleR regulatory protein from Lactococcus lactis, which is involved in the expression of the malolactic genes in the presence of L-malate. However, the MleR-like protein of L. oenos seems different from the protein of Lactococcus lactis, since no regulation of the malolactic enzyme by L-malate was effective under our experimental conditions.

journal_name

Appl Environ Microbiol

authors

Labarre C,Diviès C,Guzzo J

doi

10.1128/AEM.62.12.4493-4498.1996

subject

Has Abstract

pub_date

1996-12-01 00:00:00

pages

4493-8

issue

12

eissn

0099-2240

issn

1098-5336

journal_volume

62

pub_type

杂志文章
  • Elution of viruses by ionic and nonionic surfactants.

    abstract::The ionic and nonionic surfactants sodium dodecyl sulfate and Triton X-100, respectively, eluted two viruses, phi X174 and PRD1, which were adsorbed to the ionic and nonionic binding membranes cationic polysulfone and nitrocellulose, respectively. Results indicated that complete elution was readily achieved only when ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.9.3470-3473.1996

    authors: Fujito BT,Lytle CD

    更新日期:1996-09-01 00:00:00

  • Effect of plating medium on heat activation requirement of Clostridium botulinum spores.

    abstract::Clostridium botulinum 62A and ATCC 25763 spores required heat activation for maximum colony formation when plated on reinforced clostridial agar (BBL Microbiology Systems) but not when plated on botulinum assay medium. Spores from strains B-aphis and 53B did not exhibit heat activation when plated on either medium. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.42.4.734-736.1981

    authors: Montville TJ

    更新日期:1981-10-01 00:00:00

  • Effects of Agricultural Management on Rhizosphere Microbial Structure and Function in Processing Tomato Plants.

    abstract::Agricultural management practices affect bulk soil microbial communities and the functions they carry out, but it remains unclear how these effects extend to the rhizosphere in different agroecosystem contexts. Given close linkages between rhizosphere processes and plant nutrition and productivity, understanding how m...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01064-19

    authors: Schmidt JE,Vannette RL,Igwe A,Blundell R,Casteel CL,Gaudin ACM

    更新日期:2019-08-01 00:00:00

  • Growth of the fungus Cladosporium sphaerospermum with toluene as the sole carbon and energy source.

    abstract::The fungus Cladosporium sphaerospermum was isolated from a biofilter used for the removal of toluene from waste gases. This is the first report describing growth of a eukaryotic organism with toluene as the sole source of carbon and energy. The oxygen consumption rates, as well as the measured enzyme activities, of to...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.10.3562-3566.1995

    authors: Weber FJ,Hage KC,de Bont JA

    更新日期:1995-10-01 00:00:00

  • Rapid mineralization of the phenylurea herbicide diuron by Variovorax sp. strain SRS16 in pure culture and within a two-member consortium.

    abstract::The phenylurea herbicide diuron [N-(3,4-dichlorophenyl)-N,N-dimethylurea] is widely used in a broad range of herbicide formulations, and consequently, it is frequently detected as a major water contaminant in areas where there is extensive use. We constructed a linuron [N-(3,4-dichlorophenyl)-N-methoxy-N-methylurea]- ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02687-07

    authors: Sørensen SR,Albers CN,Aamand J

    更新日期:2008-04-01 00:00:00

  • Identification of a malate chemoreceptor in Pseudomonas aeruginosa by screening for chemotaxis defects in an energy taxis-deficient mutant.

    abstract::We found that a robust energy taxis response mediated by the Aer receptor can sometimes mask chemotaxis mediated by other methyl-accepting chemotaxis proteins (MCPs) in Pseudomonas aeruginosa. We identified PA2652 as a chemoreceptor for malate by screening aer mcp double mutants by using swarm plate assays. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01898-07

    authors: Alvarez-Ortega C,Harwood CS

    更新日期:2007-12-01 00:00:00

  • Sensitivity of aflatoxin b1 to ionizing radiation.

    abstract::Aflatoxin B1 in a 5-micrograms/ml water solution was sensitive to ionizing radiation. Inactivation was assayed by the Ames microsome mutagenicity test and confirmed by thin-layer chromatography. Destruction of aflatoxin B1 had already begun at 2.5 kilograms (kGy; 1 kGy = 0.1 Mrad), but a dose exceeding 10 kGy was nece...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.43.6.1317-1319.1982

    authors: Van Dyck PJ,Tobback P,Feyes M,van de Voorde H

    更新日期:1982-06-01 00:00:00

  • Copper-resistant enteric bacteria from United Kingdom and Australian piggeries.

    abstract::Thirty-three enteric isolates from Australian (Escherichia coli only) and United Kingdom (U.K.) (Salmonella sp., Citrobacter spp., and E. coli) piggeries were characterized with respect to their copper resistance. The copper resistance phenotypes of four new Australian E. coli isolates were comparable with that of the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.8.2531-2537.1993

    authors: Williams JR,Morgan AG,Rouch DA,Brown NL,Lee BT

    更新日期:1993-08-01 00:00:00

  • Heat Resistance Correlated with DNA Content in Bacillus megaterium Spores.

    abstract::Two subpopulations of Bacillus megaterium spores (1.360 and 1.355 g/ml) were obtained by density gradient centrifugation. The heavier spores had a higher thermoresistance (e.g., D(80) = 186 versus 81 min) and a higher DNA content (1.25 x 10 versus 0.65 x 10 g per spore, apparently corresponding to digenomic versus mon...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.9.2919-2921.1990

    authors: Belliveau BH,Beaman TC,Gerhardt P

    更新日期:1990-09-01 00:00:00

  • Characterization of a Dehalobacter coculture that dechlorinates 1,2-dichloroethane to ethene and identification of the putative reductive dehalogenase gene.

    abstract::Dehalobacter and "Dehalococcoides" spp. were previously shown to be involved in the biotransformation of 1,1,2-trichloroethane (1,1,2-TCA) and 1,2-dichloroethane (1,2-DCA) to ethene in a mixed anaerobic enrichment culture. Here we report the further enrichment and characterization of a Dehalobacter sp. from this mixed...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02037-08

    authors: Grostern A,Edwards EA

    更新日期:2009-05-01 00:00:00

  • Diversity of microcystin-producing cyanobacteria in spatially isolated regions of Lake Erie.

    abstract::The diversity of microcystin-producing cyanobacteria in the western basin of Lake Erie was studied using sequence analysis of mcyA gene fragments. Distinct populations of potentially toxic Microcystis and Planktothrix were found in spatially isolated locations. This study highlights previously undocumented diversity o...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00312-06

    authors: Rinta-Kanto JM,Wilhelm SW

    更新日期:2006-07-01 00:00:00

  • Relative importance of fluorescent siderophores and other factors in biological control of Gaeumannomyces graminis var. tritici by Pseudomonas fluorescens 2-79 and M4-80R.

    abstract::Pseudomonas fluorescens 2-79 suppresses take-all, a major root disease of wheat caused by Gaeumannomyces graminis var. tritici. The bacteria produce an antibiotic, phenazine-1-carboxylic acid (PCA), and a fluorescent pyoverdin siderophore. Previous studies have established that PCA has an important role in the biologi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.11.3270-3277.1991

    authors: Hamdan H,Weller DM,Thomashow LS

    更新日期:1991-11-01 00:00:00

  • Isolation of novel extreme-tolerant cyanobacteria from a rock-dwelling microbial community by using exposure to low Earth orbit.

    abstract::Many cyanobacteria are known to tolerate environmental extremes. Motivated by an interest in selecting cyanobacteria for applications in space, we launched rocks from a limestone cliff in Beer, Devon, United Kingdom, containing an epilithic and endolithic rock-dwelling community of cyanobacteria into low Earth orbit (...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02547-09

    authors: Olsson-Francis K,de la Torre R,Cockell CS

    更新日期:2010-04-01 00:00:00

  • Physiological Properties of a Mutant of Pachysolen tannophilus Deficient in NADPH-Dependent d-Xylose Reductase.

    abstract::A d-xylose reductase mutant of Pachysolen tannophilus was isolated on the basis of its poor growth on d-xylose but normal growth on xylitol and d-glucose. Fractionation of cell extracts indicated that the mutant was deficient in d-xylose reductase activity that used NADPH exclusively as a cofactor, but not in activity...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.11.2877-2881.1989

    authors: Schneider H,Lee H,Barbosa Mde F,Kubicek CP,James AP

    更新日期:1989-11-01 00:00:00

  • Factors limiting microbial growth and activity at a proposed high-level nuclear repository, yucca mountain, nevada.

    abstract::As part of the characterization of Yucca Mountain, Nev., as a potential repository for high-level nuclear waste, volcanic tuff was analyzed for microbial abundance and activity. Tuff was collected aseptically from nine sites along a tunnel in Yucca Mountain. Microbial abundance was generally low: direct microscopic ce...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.8.3128-3133.1997

    authors: Kieft TL,Kovacik WP,Ringelberg DB,White DC,Haldeman DL,Amy PS,Hersman LE

    更新日期:1997-08-01 00:00:00

  • Conjugative transfer between Escherichia coli and Leptospira spp. as a new genetic tool.

    abstract::Our understanding of leptospiral pathogenesis, which remains poorly understood, depends on reliable genetic tools for functional analysis of genes in pathogenic strains. In this study, we report the first demonstration of conjugation between Escherichia coli and Leptospira spp. by using RP4 derivative conjugative plas...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02172-07

    authors: Picardeau M

    更新日期:2008-01-01 00:00:00

  • Osmoregulation in symbiosis-independent mutants of Bdellovibrio bacteriovorus.

    abstract::Bdellovibrios capable of axenic growth grow in a cell-free medium at a rate considerably lower than that attainable in a two-membered culture with Escherichia coli. The axenic growth rate may be improved either by adjustment of the osmosity of the medium or by the addition of low concentrations of spermine. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.33.5.1207-1208.1977

    authors: Varon M,Seijffers J

    更新日期:1977-05-01 00:00:00

  • Bacterial leaching of metal sulfides proceeds by two indirect mechanisms via thiosulfate or via polysulfides and sulfur

    abstract::The acid-insoluble metal sulfides FeS2, MoS2, and WS2 are chemically attacked by iron(III) hexahydrate ions, generating thiosulfate, which is oxidized to sulfuric acid. Other metal sulfides are attacked by iron(III) ions and by protons, resulting in the formation of elemental sulfur via intermediary polysulfides. Sulf...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.1.319-321.1999

    authors: Schippers A,Sand W

    更新日期:1999-01-01 00:00:00

  • Histopathology of Mycotoxicosis produced in Swiss albino mice by metabolites of some fungal isolates.

    abstract::Of 199 fungal cultures isolated from some of the common cereals collected from different parts of Uttar Pradesh and Madhya Pradesh, 70 produced toxic metabolites. Of the 70 fungi isolated, 59 produced toxins which caused visible lesions in livers, kidneys, and spleens but did not cause mortality. Toxicity, graded in t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.41.3.752-757.1981

    authors: Gupta J,Pathak B,Sethi N,Vora VC

    更新日期:1981-03-01 00:00:00

  • Phylogenetic analysis of Bacillus subtilis strains applicable to natto (fermented soybean) production.

    abstract::Spore-forming Bacillus strains that produce extracellular poly-γ-glutamic acid were screened for their application to natto (fermented soybean food) fermentation. Among the 424 strains, including Bacillus subtilis and B. amyloliquefaciens, which we isolated from rice straw, 59 were capable of fermenting natto. Biotin ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00448-11

    authors: Kubo Y,Rooney AP,Tsukakoshi Y,Nakagawa R,Hasegawa H,Kimura K

    更新日期:2011-09-01 00:00:00

  • Engineered cyanophycin synthetase (CphA) from Nostoc ellipsosporum confers enhanced CphA activity and cyanophycin accumulation to Escherichia coli.

    abstract::The cyanophycin (CGP) synthetase gene (cphANE1) of the transposon-induced argL mutant NE1 of the cyanobacterium Nostoc ellipsosporum, which exhibits a CGP-leaky phenotype during diazotrophical growth, was cloned and expressed in Escherichia coli strain TOP10. Its amino acid sequence exhibited high similarities to CphA...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01132-06

    authors: Hai T,Frey KM,Steinbüchel A

    更新日期:2006-12-01 00:00:00

  • Impact of Classical Strain Improvement of Penicillium rubens on Amino Acid Metabolism during β-Lactam Production.

    abstract::To produce high levels of β-lactams, the filamentous fungus Penicillium rubens (previously named Penicillium chrysogenum) has been subjected to an extensive classical strain improvement (CSI) program during the last few decades. This has led to the accumulation of many mutations that were spread over the genome. Detai...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01561-19

    authors: Wu M,Crismaru CG,Salo O,Bovenberg RAL,Driessen AJM

    更新日期:2020-01-21 00:00:00

  • Molecular evidence for multiple infections as revealed by typing of Asaia bacterial symbionts of four mosquito species.

    abstract::The recent increased detection of acetic acid bacteria (AAB) of the genus Asaia as symbionts of mosquitoes, such as Anopheles spp. and Aedes spp., prompted us to investigate the diversity of these symbionts and their relationships in different mosquito species and populations. Following cultivation-dependent and -inde...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01747-10

    authors: Chouaia B,Rossi P,Montagna M,Ricci I,Crotti E,Damiani C,Epis S,Faye I,Sagnon N,Alma A,Favia G,Daffonchio D,Bandi C

    更新日期:2010-11-01 00:00:00

  • Production and secretion in Escherichia coli of hepatitis B virus pre-S2 antigen as fusion proteins with beta-lactamase.

    abstract::The diagnostically important surface antigen pre-S2 of hepatitis B virus was produced in large amounts in the periplasmic space of Escherichia coli. The DNA fragments (pre-S2) coding the pre-S2 antigen were tandemly duplicated or triplicated and ligated in the same reading frame to a fragment containing the promoter a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.9.2742-2747.1990

    authors: Kadokura H,Yoda K,Imai M,Yamasaki M

    更新日期:1990-09-01 00:00:00

  • Lectin-like constituents of foods which react with components of serum, saliva, and Streptococcus mutans.

    abstract::Hot and cold aqueous extracts were prepared from 22 commonly ingested fruits, vegetables, and seeds. When tested by agar diffusion, extracts from 13 and 10 of the foods formed precipitin bands with samples of normal rabbit serum and human saliva, respectively; extracts from four of the foods also reacted with antigen ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.41.4.880-888.1981

    authors: Gibbons RJ,Dankers I

    更新日期:1981-04-01 00:00:00

  • Stimulating the in situ activity of Geobacter species to remove uranium from the groundwater of a uranium-contaminated aquifer.

    abstract::The potential for removing uranium from contaminated groundwater by stimulating the in situ activity of dissimilatory metal-reducing microorganisms was evaluated in a uranium-contaminated aquifer located in Rifle, Colo. Acetate (1 to 3 mM) was injected into the subsurface over a 3-month period via an injection gallery...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.10.5884-5891.2003

    authors: Anderson RT,Vrionis HA,Ortiz-Bernad I,Resch CT,Long PE,Dayvault R,Karp K,Marutzky S,Metzler DR,Peacock A,White DC,Lowe M,Lovley DR

    更新日期:2003-10-01 00:00:00

  • Detoxification of corn antimicrobial compounds as the basis for isolating Fusarium verticillioides and some other Fusarium species from corn.

    abstract::The preformed antimicrobial compounds produced by maize, 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3-one and its desmethoxy derivative 2,4-dihydroxy-2H-1,4-benzoxazin-3-one, are highly reactive benzoxazinoids that quickly degrade to the antimicrobials 6-methoxy-2-benzoxazolinone (MBOA) and 2-benzoxazolinone (BOA), res...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.7.2973-2981.2001

    authors: Glenn AE,Hinton DM,Yates IE,Bacon CW

    更新日期:2001-07-01 00:00:00

  • Different temperature optima for methane formation when enrichments from Acid peat are supplemented with acetate or hydrogen.

    abstract::Laboratory studies of methane formation in peat samples from an acid subarctic mire in Sweden indicated the presence of a low-temperature-adapted methanogenic flora. Enrichment culture studies with ethanol, acetate, hydrogen, or a combination of these as substrate for methane formation provided evidence for the existe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.48.2.389-394.1984

    authors: Svensson BH

    更新日期:1984-08-01 00:00:00

  • Soil Aggregate Microbial Communities: Towards Understanding Microbiome Interactions at Biologically Relevant Scales.

    abstract::Soils contain a tangle of minerals, water, nutrients, gases, plant roots, decaying organic matter, and microorganisms which work together to cycle nutrients and support terrestrial plant growth. Most soil microorganisms live in periodically interconnected communities closely associated with soil aggregates, i.e., smal...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1128/AEM.00324-19

    authors: Wilpiszeski RL,Aufrecht JA,Retterer ST,Sullivan MB,Graham DE,Pierce EM,Zablocki OD,Palumbo AV,Elias DA

    更新日期:2019-07-01 00:00:00

  • Isolation and characterization of a novel bacterium growing via reductive dehalogenation of 2-chlorophenol.

    abstract::A bacterium capable of anaerobic growth via reductive dehalogenation of 2-chlorophenol was isolated from a culture enriched from sediment taken from a small stream near Lansing, Mich. The organism, designated strain 2CP-1, is a gram-negative rod ca. 3 by 0.5 micron in size and is a catalase-negative, oxidase-negative,...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.10.3536-3542.1994

    authors: Cole JR,Cascarelli AL,Mohn WW,Tiedje JM

    更新日期:1994-10-01 00:00:00