Physiological Properties of a Mutant of Pachysolen tannophilus Deficient in NADPH-Dependent d-Xylose Reductase.

abstract：:Due to concerns about a link between variant Creutzfeldt-Jakob disease in humans and similar prion protein-induced disease in cattle, i.e., bovine spongiform encephalopathy (BSE), strict controls are in place to exclude BSE-positive animals and/or specified risk materials including bovine central nervous system (CNS) ...

journal_title：Applied and environmental microbiology

authors： Daly DJ,Prendergast DM,Sheridan JJ,Blair IS,McDowell DA

更新日期：2002-02-01 00:00:00

abstract：:Deletion of the pcaHG genes, encoding protocatechuate 3,4-dioxygenase in Rhodococcus jostii RHA1, gives a gene deletion strain still able to grow on protocatechuic acid as the sole carbon source, indicating a second degradation pathway for protocatechuic acid. Metabolite analysis of wild-type R. jostii RHA1 grown on m...

journal_title：Applied and environmental microbiology

authors： Spence EM,Scott HT,Dumond L,Calvo-Bado L,di Monaco S,Williamson JJ,Persinoti GF,Squina FM,Bugg TDH

更新日期：2020-09-17 00:00:00

abstract：:The nucleotide sequence of an endo-beta-1,4-glucanase gene of Clostridium acetobutylicum contained two putative extended promoter consensus sequences, a Shine-Dalgarno sequence and a TTG initiation codon. The nucleotide sequence of the gene coding for the C-terminal region of this enzyme was not required for activity....

journal_title：Applied and environmental microbiology

authors： Zappe H,Jones WA,Jones DT,Woods DR

更新日期：1988-05-01 00:00:00

abstract：:Pseudomonas fluorescens Pf-5 is a soil bacterium that suppresses plant pathogens due in part to its production of the antibiotic pyoluteorin. Previous characterization of Pf-5 revealed three global regulators, including the stationary-phase sigma factor sigma(S) and the two-component regulators GacA and GacS, that inf...

journal_title：Applied and environmental microbiology

authors： Whistler CA,Stockwell VO,Loper JE

更新日期：2000-07-01 00:00:00

abstract：:The nor-1 gene in the filamentous fungus Aspergillus parasiticus encodes a ketoreductase involved in aflatoxin biosynthesis. To study environmental influences on nor-1 expression, we generated plasmid pAPGUSNNB containing a nor-1 promoter-beta-glucuronidase (GUS) (encoded by uidA) reporter fusion with niaD (encodes ni...

journal_title：Applied and environmental microbiology

authors： Chiou CH,Miller M,Wilson DL,Trail F,Linz JE

更新日期：2002-01-01 00:00:00

abstract：:The diversity of the predominant bacteria in the human gastrointestinal tract was studied by using 16S rRNA-based approaches. PCR amplicons of the V6 to V8 regions of fecal 16S rRNA and ribosomal DNA (rDNA) were analyzed by temperature gradient gel electrophoresis (TGGE). TGGE of fecal 16S rDNA amplicons from 16 indiv...

journal_title：Applied and environmental microbiology

authors： Zoetendal EG,Akkermans AD,De Vos WM

更新日期：1998-10-01 00:00:00

abstract：:The molecular epidemiology of 545 Salmonella enterica serovar Typhimurium isolates collected between 1977 and 2009 from cattle in Hokkaido, Japan, was investigated using pulsed-field gel electrophoresis (PFGE). Nine main clusters were identified from 116 PFGE patterns. Cluster I comprised 248 isolates, 243 of which po...

journal_title：Applied and environmental microbiology

authors： Tamamura Y,Uchida I,Tanaka K,Okazaki H,Tezuka S,Hanyu H,Kataoka N,Makino S,Kishima M,Kubota T,Kanno T,Hatama S,Ishihara R,Hata E,Yamada H,Nakaoka Y,Akiba M

更新日期：2011-03-01 00:00:00

abstract：:We report the characterization of the ccpA gene of Lactobacillus plantarum, coding for catabolite control protein A. The gene is linked to the pepQ gene, encoding a proline peptidase, in the order ccpA-pepQ, with the two genes transcribed in tandem from the same strand as distinct transcriptional units. Two ccpA trans...

journal_title：Applied and environmental microbiology

authors： Muscariello L,Marasco R,De Felice M,Sacco M

更新日期：2001-07-01 00:00:00

abstract：:A new primer set was designed to specifically amplify ca. 1,100 bp of aoxB genes encoding the As(III) oxidase catalytic subunit from taxonomically diverse aerobic As(III)-oxidizing bacteria. Comparative analysis of AoxB protein sequences showed variable conservation levels and highlighted the conservation of essential...

journal_title：Applied and environmental microbiology

authors： Quéméneur M,Heinrich-Salmeron A,Muller D,Lièvremont D,Jauzein M,Bertin PN,Garrido F,Joulian C

更新日期：2008-07-01 00:00:00

abstract：:beta-D-Galactosidase (EC 3.2.1.23) was extracted from Streptococcus thermophilus grown in deproteinized cheese whey. Cultural conditions optimum for maximum enzyme production were pH 7.0, 40 degrees C, and 24 h. Proteose peptone (2.0%, wt/vol) and corn steep liquor (2.8%, wt/vol) were highly stimulatory, increasing th...

journal_title：Applied and environmental microbiology

authors： Rao MV,Dutta SM

更新日期：1977-08-01 00:00:00

abstract：:Escherichia coli O157:H7 strains can be classified into different genotypes based on the presence of specific Shiga toxin-encoding bacteriophage insertion sites. Certain O157:H7 genotypes predominate among human clinical cases (clinical genotypes), while others are more frequently found in bovines (bovine-biased genot...

journal_title：Applied and environmental microbiology

authors： Vanaja SK,Springman AC,Besser TE,Whittam TS,Manning SD

更新日期：2010-01-01 00:00:00

abstract：:A previously identified insert expressing an endopeptidase from a Lactobacillus helveticus CNRZ32 genomic library was characterized. Nucleotide sequence analysis revealed an open reading frame of 1,941 bp encoding a putative protein of 71.2 kDa which contained a zinc-protease motif. Protein homology searches revealed ...

journal_title：Applied and environmental microbiology

authors： Chen YS,Steele JL

更新日期：1998-09-01 00:00:00

abstract：:Recombinant DNA clones carrying high-copy or low-copy sequences from Aspergillus nidulans and Neurospora crassa were used to identify restriction fragment length polymorphisms (RFLPs) diagnostic for members of the A. flavus group: A. flavus, A. parasiticus, and A. nomius. These fungi were resolved into three distinct ...

journal_title：Applied and environmental microbiology

authors： Moody SF,Tyler BM

更新日期：1990-08-01 00:00:00

abstract：:Secondary sewage effluent and renovated water from four wells at the Flushing Meadows Wastewater Renovation Project near Phoenix, Arizona, in operation since 1967, were assayed approximately every 2 months in 1974 for viruses and enteric bacteria during flooding periods. No viruses of Salmonella sp. were detected in a...

journal_title：Applied and environmental microbiology

authors： Gilbert RG,Gerba CP,Rice RC,Bouwer H,Wallis C,Melnick JL

更新日期：1976-09-01 00:00:00

abstract：:Two acetyl esterases (EC 3.1.1.6) were purified to gel electrophoretic homogeneity from Thermoanaerobacterium sp. strain JW/SL-YS485, an anaerobic, thermophilic endospore former which is able to utilize various substituted xylans for growth. Both enzymes released acetic acid from chemically acetylated larch xylan. Ace...

journal_title：Applied and environmental microbiology

authors： Shao W,Wiegel J

更新日期：1995-02-01 00:00:00

abstract：:The Bacteria community composition in an acidic Sphagnum peat bog (pH 3.9 to 4.5) was characterized by a combination of 16S rRNA gene clone library analysis, rRNA-targeted fluorescence in situ hybridization (FISH), and cultivation. Among 84 environmental 16S rRNA gene clones, a set of only 16 cloned sequences was clos...

journal_title：Applied and environmental microbiology

authors： Dedysh SN,Pankratov TA,Belova SE,Kulichevskaya IS,Liesack W

更新日期：2006-03-01 00:00:00

abstract：:Listeria monocytogenes does not produce siderophores for iron acquisition. We demonstrate that a number of microbial siderophores and natural iron-binding compounds are able to promote the growth of iron-starved L. monocytogenes. We suggest that the ability of L. monocytogenes to use a variety of exogenous siderophore...

journal_title：Applied and environmental microbiology

authors： Simon N,Coulanges V,Andre P,Vidon DJ

更新日期：1995-04-01 00:00:00

abstract：:Denitrifying microbial communities and denitrification in salt marsh sediments may be affected by many factors, including environmental conditions, nutrient availability, and levels of pollutants. The objective of this study was to examine how microbial community composition and denitrification enzyme activities (DEA)...

journal_title：Applied and environmental microbiology

authors： Cao Y,Green PG,Holden PA

更新日期：2008-12-01 00:00:00

abstract：:The human gastrointestinal (GI) tract provides home to a complex microbial community, collectively termed microbiota. Although major efforts have been made to describe the diversity and stability of the microbiota, functional studies have been largely restricted to intestinal isolates and include few community studies...

journal_title：Applied and environmental microbiology

authors： Booijink CC,Boekhorst J,Zoetendal EG,Smidt H,Kleerebezem M,de Vos WM

更新日期：2010-08-01 00:00:00

abstract：:The supernatant from centrifugation at 1,000 x g of strained rumen fluid was lyophilized, and the residue and sublimate fractions were used to replace fresh rumen fluid in a complete roll tube medium for enumeration of total rumen bacteria. Most of the growth-supporting nutrients in fresh rumen fluid were found in the...

journal_title：Applied and environmental microbiology

authors： Dehority BA,Tirabasso PA

更新日期：1989-12-01 00:00:00

abstract：:The objective of this study was to develop a molecular detection method that better estimates the potential risk associated with the presence of Vibrio vulnificus. For that purpose, we applied seminested reverse transcription-PCR (RT-PCR) to viable but nonculturable (VBNC) populations of V. vulnificus and targeted the...

journal_title：Applied and environmental microbiology

authors： Fischer-Le Saux M,Hervio-Heath D,Loaec S,Colwell RR,Pommepuy M

更新日期：2002-11-01 00:00:00

abstract：:In the current study, we adapted and optimized the lambda Red recombineering strategy to genetically manipulate the fastidious insect endosymbiont Sodalis glossinidius. This work greatly facilitates the application of genetics to the study of insect symbionts and should also prove useful in the context of long-awaited...

journal_title：Applied and environmental microbiology

authors： Pontes MH,Dale C

更新日期：2011-03-01 00:00:00

abstract：:To ascertain its physiological similarity to other methanogenic bacteria, Methanospirillum hungatii, the type species of the genus, was characterized nutritionally and biochemically. Good growth occurred in a medium consisting of mineral salts, cysteine sulfide reducing buffer, and an H2-CO2 (80:20) atmosphere. Additi...

journal_title：Applied and environmental microbiology

authors： Ferry JG,Wolfe RS

更新日期：1977-10-01 00:00:00

abstract：:Stability of foreign DNA transformed into a novel host is an important parameter in decisions to permit the release of genetically engineered microorganisms into the environment. Meiotic instability of transformed DNA has been reported in fungi such as Ascobolus, Aspergillus, and Neurospora. We used strains of Gibbere...

journal_title：Applied and environmental microbiology

authors： Leslie JF,Dickman MB

更新日期：1991-05-01 00:00:00

abstract：:Previous studies implicated loss of motility and mutations of the alsR and sigW regulatory genes in enhanced fitness of the Bacillus subtilis evolved strain WN716 over that of its ancestral strain WN624. The fitness of strains carrying knockout mutations alsR::spc, sigD::kan, and/or sigW::erm was measured and compared...

journal_title：Applied and environmental microbiology

authors： Nicholson WL

更新日期：2012-05-01 00:00:00

abstract：:Reductive and oxidative pathways of the sulfur cycle were studied in a marine sediment by parallel radiotracer experiments with SO(4), H(2)S, and S(2)O(3) injected into undisturbed sediment cores. The distributions of viable populations of sulfate- and thiosulfate-reducing bacteria and of thiosulfate-disproportionatin...

journal_title：Applied and environmental microbiology

authors： Jørgensen BB,Bak F

更新日期：1991-03-01 00:00:00

abstract：:Direct electron microscopy of bacteriophages adsorbed to a carbon film without prior enrichment by specific host strains or concentration by physical or chemical methods was used to study the morphological diversity of natural bacteriophage assemblages in a North German lake. All samples contained a mixture of morphol...

journal_title：Applied and environmental microbiology

authors： Demuth J,Neve H,Witzel KP

更新日期：1993-10-01 00:00:00

abstract：:CRISPR-based systems are emerging as the premier method to manipulate many cellular processes. In this study, a simple and efficient CRISPR interference (CRISPRi) system for targeted gene repression in archaea was developed. The Methanosarcina acetivorans CRISPR-Cas9 system was repurposed by replacing Cas9 with the ca...

journal_title：Applied and environmental microbiology

authors： Dhamad AE,Lessner DJ

更新日期：2020-10-15 00:00:00

abstract：:Three particulate methane monooxygenase PCR primer sets (A189-A682, A189-A650, and A189-mb661) were investigated for their ability to assess methanotroph diversity in soils from three sites, i.e., heath, oak, and sitka, each of which was capable of oxidizing atmospheric concentrations of methane. Each PCR primer set w...

journal_title：Applied and environmental microbiology

authors： Bourne DG,McDonald IR,Murrell JC

更新日期：2001-09-01 00:00:00

abstract：:Determination of microbial viability by the plate count method is routine in microbiology laboratories worldwide. However, limitations of the technique, particularly with respect to environmental microorganisms, are widely recognized. Many alternatives based upon viability staining have been proposed, and these are of...

journal_title：Applied and environmental microbiology

authors： Davey HM

更新日期：2011-08-15 00:00:00