Diversity surveys and evolutionary relationships of aoxB genes in aerobic arsenite-oxidizing bacteria.

Abstract:

:A new primer set was designed to specifically amplify ca. 1,100 bp of aoxB genes encoding the As(III) oxidase catalytic subunit from taxonomically diverse aerobic As(III)-oxidizing bacteria. Comparative analysis of AoxB protein sequences showed variable conservation levels and highlighted the conservation of essential amino acids and structural motifs. AoxB phylogeny of pure strains showed well-discriminated taxonomic groups and was similar to 16S rRNA phylogeny. Alphaproteobacteria-, Betaproteobacteria-, and Gammaproteobacteria-related sequences were retrieved from environmental surveys, demonstrating their prevalence in mesophilic As-contaminated soils. Our study underlines the usefulness of the aoxB gene as a functional marker of aerobic As(III) oxidizers.

journal_name

Appl Environ Microbiol

authors

Quéméneur M,Heinrich-Salmeron A,Muller D,Lièvremont D,Jauzein M,Bertin PN,Garrido F,Joulian C

doi

10.1128/AEM.02851-07

subject

Has Abstract

pub_date

2008-07-01 00:00:00

pages

4567-73

issue

14

eissn

0099-2240

issn

1098-5336

pii

AEM.02851-07

journal_volume

74

pub_type

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