Extracellular protease of Pseudomonas fluorescens CHA0, a biocontrol factor with activity against the root-knot nematode Meloidogyne incognita.

abstract：:The ability of fractions of dissolved organic carbon (DOC) of different molecular weights (MW) to support bacterial growth was studied in batch culture experiments. Natural pelagic bacteria were inoculated into particle-free (0.2-mum filtered) water, taken from 10 oligotrophic lakes of differing humic content, and eit...

journal_title：Applied and environmental microbiology

authors： Tranvik LJ

更新日期：1990-06-01 00:00:00

abstract：:Lactococcin G and enterocin 1071 are two homologous two-peptide bacteriocins. Expression vectors containing the gene encoding the putative lactococcin G immunity protein (lagC) or the gene encoding the enterocin 1071 immunity protein (entI) were constructed and introduced into strains sensitive to one or both of the b...

journal_title：Applied and environmental microbiology

authors： Oppegård C,Emanuelsen L,Thorbek L,Fimland G,Nissen-Meyer J

更新日期：2010-02-01 00:00:00

abstract：:The internal pH of Clostridium acetobutylicum was determined at various stages during the growth of the organism. Even in the presence of significant quantities of acetic, butyric, and lactic acids, an internal pH of 6.2 was maintained. Experiments using N,N'-dicyclohexylcarbodiimide indicated that a functioning H+-AT...

journal_title：Applied and environmental microbiology

authors： Bowles LK,Ellefson WL

更新日期：1985-11-01 00:00:00

abstract：:A triplex reverse transcriptase PCR (RT-PCR) was developed to simultaneously detect poliovirus, hepatitis A virus (HAV), and rotavirus in sewage and ocean water. Sewage and ocean water samples seeded with the three different viruses were concentrated by ultrafiltration. The unseeded ocean water and sewage samples were...

journal_title：Applied and environmental microbiology

authors： Tsai YL,Tran B,Sangermano LR,Palmer CJ

更新日期：1994-07-01 00:00:00

abstract：:Both the periplasmic and the extracellular cellodextrinases from Bacteroides succinogenes S85 grown on Avicel microcrystalline cellulose were purified to homogeneity by column chromatography and characterized. Over 70% of the total cellobiosidase activity displayed by cells was accounted for by these enzymes. The peri...

journal_title：Applied and environmental microbiology

authors： Huang L,Forsberg CW

更新日期：1988-06-01 00:00:00

abstract：:The diversity and dynamics of a bacterial community extracted from an exploited oil field with high natural soil salinity near Comodoro Rivadavia in Patagonia (Argentina) were investigated. Community shifts during long-term incubation with diesel fuel at four salinities between 0 and 20% NaCl were monitored by single-...

journal_title：Applied and environmental microbiology

authors： Kleinsteuber S,Riis V,Fetzer I,Harms H,Müller S

更新日期：2006-05-01 00:00:00

abstract：:Dairy propionic acid bacteria, particularly the species Propionibacterium freudenreichii, play a major role in the ripening of Swiss type cheese. Isometric and filamentous bacteriophages infecting P. freudenreichii have previously been isolated from cheese. In order to determine the origin of these bacteriophages, lys...

journal_title：Applied and environmental microbiology

authors： Hervé C,Coste A,Rouault A,Fraslin JM,Gautier M

更新日期：2001-01-01 00:00:00

abstract：:Integrated air sampling and PCR-based methods for detecting airborne fungal spores, using Penicillium roqueforti as a model fungus, are described. P. roqueforti spores were collected directly into Eppendorf tubes using a miniature cyclone-type air sampler. They were then suspended in 0.1% Nonidet P-40, and counted usi...

journal_title：Applied and environmental microbiology

authors： Williams RH,Ward E,McCartney HA

更新日期：2001-06-01 00:00:00

abstract：:As part of the characterization of Yucca Mountain, Nev., as a potential repository for high-level nuclear waste, volcanic tuff was analyzed for microbial abundance and activity. Tuff was collected aseptically from nine sites along a tunnel in Yucca Mountain. Microbial abundance was generally low: direct microscopic ce...

journal_title：Applied and environmental microbiology

authors： Kieft TL,Kovacik WP,Ringelberg DB,White DC,Haldeman DL,Amy PS,Hersman LE

更新日期：1997-08-01 00:00:00

abstract：:Rhodococcus erythropolis N1-36, a desulfurization strain, was grown in continuous culture at 10 different dilution rates with 50 (mu)M dibenzothiophene sulfone (DBTO(inf2)) as the growth-limiting nutrient. The steady-state biomass, concentrations of substrate (DBTO(inf2)) and product (monohydroxybiphenyl), saturation ...

journal_title：Applied and environmental microbiology

authors： Wang P,Humphrey AE,Krawiec S

更新日期：1996-08-01 00:00:00

abstract：:The control of promoter activity by oxygen availability appears to be an intriguing system for heterologous protein production. In fact, during cell growth in a bioreactor, an oxygen shortage is easily obtained simply by interrupting the air supply. The purpose of our work was to explore the possible use of hypoxic in...

journal_title：Applied and environmental microbiology

authors： Camattari A,Bianchi MM,Branduardi P,Porro D,Brambilla L

更新日期：2007-02-01 00:00:00

abstract：:We describe here the development of a single-reaction multiplex PCR assay for the enterotoxin genes from Staphylococcus aureus that utilizes a universal toxin gene primer in combination with toxin-specific primers to amplify characteristic toxin gene products. In combination with a new DNA purification method, the ass...

journal_title：Applied and environmental microbiology

authors： Sharma NK,Rees CE,Dodd CE

更新日期：2000-04-01 00:00:00

abstract：:The nutristat, a substrate concentration-controlled continuous culture, was used to grow pentachlorophenol (PCP)-degrading microorganisms. The PCP concentration control system consisted of on-line measurement of the PCP concentration in the culture vessel with a tangential filter and a flowthrough spectrophotometer. W...

journal_title：Applied and environmental microbiology

authors： Rutgers M,Bogte JJ,Breure AM,van Andel JG

更新日期：1993-10-01 00:00:00

abstract：:The harvesting of rainwater is gaining acceptance among many governmental authorities in countries such as Australia, Germany, and South Africa, among others. However, conflicting reports on the microbial quality of harvested rainwater have been published. To monitor the presence of potential pathogenic bacteria durin...

journal_title：Applied and environmental microbiology

authors： Dobrowsky PH,De Kwaadsteniet M,Cloete TE,Khan W

更新日期：2014-04-01 00:00:00

abstract：:The significance of dissolved combined amino acids (DCAA), dissolved free amino acids (DFAA), and dissolved DNA (D-DNA) as sources of C and N for marine bacteria in batch cultures with variable substrate C/N ratios was studied. Glucose, ammonium, alanine, and phosphate were added to the cultures to produce C/N ratios ...

journal_title：Applied and environmental microbiology

authors： Jørgensen NO,Kroer N,Coffin RB

更新日期：1994-11-01 00:00:00

abstract：:White-nose syndrome is a fungal disease that has decimated bat populations across eastern North America. Identification of the etiologic agent, Pseudogymnoascus destructans (formerly Geomyces destructans), in environmental samples is essential to proposed management plans. A major challenge is the presence of closely ...

journal_title：Applied and environmental microbiology

authors： Shuey MM,Drees KP,Lindner DL,Keim P,Foster JT

更新日期：2014-03-01 00:00:00

abstract：:We determined the prevalences of Escherichia coli O157:H7 in feces, hide, and carcasses of meat goats at a U.S. processing plant. Prevalences were 11.1%, 2.7%, and 2.7%, respectively. Sixteen pulsed-field gel electrophoresis (PFGE) subtypes were identified among 49 E. coli O157:H7 isolates, some of which were present ...

journal_title：Applied and environmental microbiology

authors： Jacob ME,Foster DM,Rogers AT,Balcomb CC,Sanderson MW

更新日期：2013-07-01 00:00:00

abstract：:A mutant of Pseudomonas sp. strain HBP1, originally isolated on 2-hydroxybiphenyl, was selected for the ability to grow on 2-propylphenol as the sole carbon and energy source. In the mutant strain, which was designated as Pseudomonas sp. strain HBP1 Prp, the cellular induction mechanism involved in the synthesis of th...

journal_title：Applied and environmental microbiology

authors： Kohler HP,van der Maarel MJ,Kohler-Staub D

更新日期：1993-03-01 00:00:00

abstract：:To gain a predictive understanding of the interspecies interactions within microbial communities that govern community function, the genomic complement of every member population must be determined. Although metagenomic sequencing has enabled the de novo reconstruction of some microbial genomes from environmental comm...

journal_title：Applied and environmental microbiology

authors： Nelson WC,Maezato Y,Wu YW,Romine MF,Lindemann SR

更新日期：2015-10-23 00:00:00

abstract：:We have previously reported that the prominent industrial enzyme producer Trichoderma reesei (teleomorph Hypocrea jecorina; Hypocreales, Ascomycota, Dikarya) has a genetically isolated, sympatric sister species devoid of sexual reproduction and which is constituted by the majority of anamorphic strains previously attr...

journal_title：Applied and environmental microbiology

authors： Atanasova L,Jaklitsch WM,Komoń-Zelazowska M,Kubicek CP,Druzhinina IS

更新日期：2010-11-01 00:00:00

abstract：:In in vitro estuarine water and sediment chambers, the survival of Vibrio cholerae and Escherichia coli was determined by plate counting and direct counting techniques. V. cholerae strains included environmental, clinical, and serotype O1 and non-O1 isolates, whereas E. coli strains included ATCC 25922 and a freshly c...

journal_title：Applied and environmental microbiology

authors： Hood MA,Ness GE

更新日期：1982-03-01 00:00:00

abstract：:Acclimation of a sandy soil to an air-natural gas mixture stimulated the biological oxidation of chloroform to carbon dioxide. Acetylene and methane inhibited chloroform oxidation. Chloroform oxidation continued up to 31 days in the absence of methane. Chloroform oxidation rates increased at chloroform concentrations ...

journal_title：Applied and environmental microbiology

authors： Strand SE,Shippert L

更新日期：1986-07-01 00:00:00

abstract：:Mycophenolic acid (MPA) is the active ingredient in the increasingly important immunosuppressive pharmaceuticals CellCept (Roche) and Myfortic (Novartis). Despite the long history of MPA, the molecular basis for its biosynthesis has remained enigmatic. Here we report the discovery of a polyketide synthase (PKS), MpaC,...

journal_title：Applied and environmental microbiology

authors： Regueira TB,Kildegaard KR,Hansen BG,Mortensen UH,Hertweck C,Nielsen J

更新日期：2011-05-01 00:00:00

abstract：:The biological succession that occurs during the first year of life in the gut of infants in Western countries is broadly predictable in terms of the increasing complexity of the composition of microbiotas. Less information is available about microbiotas in Asian countries, where environmental, nutritional, and cultur...

journal_title：Applied and environmental microbiology

authors： Lawley B,Otal A,Moloney-Geany K,Diana A,Houghton L,Heath AM,Taylor RW,Tannock GW

更新日期：2019-09-17 00:00:00

abstract：:Four sponge-associated Antarctic bacteria (i.e., Winogradskyella sp. strains CAL384 and CAL396, Colwellia sp. strain GW185, and Shewanella sp. strain CAL606) were selected for the highly mucous appearance of their colonies on agar plates. The production of extracellular polymeric substances (EPSs) was enhanced by a st...

journal_title：Applied and environmental microbiology

authors： Caruso C,Rizzo C,Mangano S,Poli A,Di Donato P,Finore I,Nicolaus B,Di Marco G,Michaud L,Lo Giudice A

更新日期：2018-01-31 00:00:00

abstract：:Our previous genetic analysis (J. W. Willis, J. K. Engwall, and A. K. Chatterjee, Phytopathology 77:1199-1205, 1987) had revealed a tight linkage between pel-3 (pel, pectate lyase gene) and peh-1 (peh, polygalacturonase gene) within the chromosome of Erwinia carotovora subsp. carotovora 71. Nucleotide sequencing, tran...

journal_title：Applied and environmental microbiology

authors： Liu Y,Chatterjee A,Chatterjee AK

更新日期：1994-07-01 00:00:00

abstract：:Strain UHM-5, a pSym Exo derivative of the aluminum-tolerant Rhizobium leguminosarum bv. phaseoli strain CIAT899, was equally tolerant of aluminum (Al) as the parental culture. Dialyzed culture supernatants of the wild-type cells grown in YEM broth (10 cells ml) contained 185 mug of glucose equivalents ml whereas UHM-...

journal_title：Applied and environmental microbiology

authors： Kingsley MT,Bohlool BB

更新日期：1992-04-01 00:00:00

abstract：:CRISPR-based systems are emerging as the premier method to manipulate many cellular processes. In this study, a simple and efficient CRISPR interference (CRISPRi) system for targeted gene repression in archaea was developed. The Methanosarcina acetivorans CRISPR-Cas9 system was repurposed by replacing Cas9 with the ca...

journal_title：Applied and environmental microbiology

authors： Dhamad AE,Lessner DJ

更新日期：2020-10-15 00:00:00

abstract：:The human large intestine is covered with a protective mucus coating, which is heavily colonized by complex bacterial populations that are distinct from those in the gut lumen. Little is known of the composition and metabolic activities of these biofilms, although they are likely to play an important role in mucus bre...

journal_title：Applied and environmental microbiology

authors： Macfarlane S,Woodmansey EJ,Macfarlane GT

更新日期：2005-11-01 00:00:00

abstract：:Microbial transformation of lapachol, a naturally occurring naphthoquinone, was carried out by Curvularia lunata (NRRL 2178). The fungus brings about oxidative cyclization of the substrate to dehydro-alpha-lapachone, which was isolated and characterized by nuclear magnetic resonance and mass spectral analyses; its str...

journal_title：Applied and environmental microbiology

authors： Otten S,Rosazza JP

更新日期：1979-08-01 00:00:00