Development of a single-reaction multiplex PCR toxin typing assay for Staphylococcus aureus strains.

abstract：:The biodegradation of polycyclic aromatic hydrocarbon pollutants is constrained, in part, by their solid physical state and very low water solubility. Searching for ways to overcome these limitations, we isolated from soil a bacterium capable of growing on pyrene as a sole source of carbon and energy. Acid-fast stain,...

journal_title：Applied and environmental microbiology

authors： Jimenez IY,Bartha R

更新日期：1996-07-01 00:00:00

abstract：:The obligate intracellular growth of Rickettsia prowazekii places severe restrictions on the analysis of rickettsial gene expression. With a small genome, predicted to code for 835 proteins, identifying which proteins are differentially expressed in rickettsiae that are isolated from different hosts or that vary in vi...

journal_title：Applied and environmental microbiology

authors： Tucker AM,Driskell LO,Pannell LK,Wood DO

更新日期：2011-07-01 00:00:00

abstract：:In Vibrio vulnificus, virulence for eels is associated with serovar E strains. In this study, we investigated some biological properties of purified lipopolysaccharides (LPSs) from serovar E and non-serovar E strains. Purified LPSs retained their O-polysaccharidic side chains and did not show any differences that coul...

journal_title：Applied and environmental microbiology

authors： Biosca EG,Collado RM,Oliver JD,Amaro C

更新日期：1999-02-01 00:00:00

abstract：:Agrobacterium tumefaciens C58 grown on acidic medium containing glucose and solidified with bacteriological agar expressed a virB::lacZ fusion. No expression of this fusion was observed on a similar medium which was solidified with purified agarose. The fraction from bacteriological agar which was responsible for vir ...

journal_title：Applied and environmental microbiology

authors： Loubens I,Chilton WS,Dion P

更新日期：1997-11-01 00:00:00

abstract：:The rhizobial bacterium Bradyrhizobium japonicum functions as a nitrogen-fixing symbiont of the soybean plant (Glycine max). Plants are capable of producing an oxidative burst, a rapid proliferation of reactive oxygen species (ROS), as a defense mechanism against pathogenic and symbiotic bacteria. Therefore, B. japoni...

journal_title：Applied and environmental microbiology

authors： Donati AJ,Jeon JM,Sangurdekar D,So JS,Chang WS

更新日期：2011-06-01 00:00:00

abstract：:There is growing interest in the application of human-associated fecal source identification quantitative real-time PCR (qPCR) technologies for water quality management. The transition from a research tool to a standardized protocol requires a high degree of confidence in data quality across laboratories. Data quality...

journal_title：Applied and environmental microbiology

authors： Shanks OC,Kelty CA,Oshiro R,Haugland RA,Madi T,Brooks L,Field KG,Sivaganesan M

更新日期：2016-04-18 00:00:00

abstract：:The insertion sequence IS629, which is highly prevalent in Escherichia coli O157:H7 genomes, was found to be absent in O157:H- strains, which are on a divergent pathway in the emergence of O157:H7. Although O157:H- is deficient in IS629, it permits IS629 transposition, with an excision frequency higher than that of an...

journal_title：Applied and environmental microbiology

authors： Rump LV,Fischer M,González-Escalona N

更新日期：2011-07-01 00:00:00

abstract：:Despite the increasing knowledge of Synechococcus spp. and their co-occurring cyanophages in oceanic and coastal water, little is known about their abundance, distribution, and interactions in the Chesapeake Bay estuarine ecosystem. A 5-year interannual survey shows that Synechococcus spp. and their phages are persist...

journal_title：Applied and environmental microbiology

authors： Wang K,Wommack KE,Chen F

更新日期：2011-11-01 00:00:00

abstract：:Short-chain-length-medium-chain-length polyhydroxyalkanoates were synthesized in Saccharomyces cerevisiae from intermediates of the beta-oxidation cycle by expressing the polyhydroxyalkanoate synthases from Aeromonas caviae and Ralstonia eutropha in the peroxisomes. The quantity of polymer produced was increased by us...

journal_title：Applied and environmental microbiology

authors： De Oliveira VC,Maeda I,Delessert S,Poirier Y

更新日期：2004-09-01 00:00:00

abstract：:Several Bacillus and Paenibacillus species were isolated from Fe and Mn oxide minerals precipitating at a deep subsurface oxic-anoxic interface at Henderson Molybdenum Mine, Empire, CO. The isolates were investigated for their Mn(II)-oxidizing potential and interrogated for possession of the mnxG gene, a gene that cod...

journal_title：Applied and environmental microbiology

authors： Mayhew LE,Swanner ED,Martin AP,Templeton AS

更新日期：2008-12-01 00:00:00

abstract：:Reporter gene technology was used to observe the regulation of the alginate biosynthesis gene, algC in a mucoid strain of Pseudomonas aeruginosa in developing and mature biofilms in continuous culture on Teflon and glass substrata. The plasmid pNZ63, carrying an algC-lacZ transcriptional fusion, was shown to not be di...

journal_title：Applied and environmental microbiology

authors： Davies DG,Geesey GG

更新日期：1995-03-01 00:00:00

abstract：:This paper presents a method for determining mutagenic concentrations of chemicals by using an agar diffusion assay. The method is based on the linear relationship between the amount of chemical placed at the center of the dish and the radius of the mutagenic zone. A brief theoretical discussion and experimental data ...

journal_title：Applied and environmental microbiology

authors： Awerbuch TE,Stark AA

更新日期：1979-12-01 00:00:00

abstract：:The acetylene block technique was used to assay denitrification in undisturbed sediment cores of an intertidal mud flat. Nitrogen loss due to this process was estimated at 1 to 41 kg of N/hectare (ha) per year. Anaerobic nitrate-saturated slurry of the same sediment had a denitrification capacity of 1,026 kg of N/ha p...

journal_title：Applied and environmental microbiology

authors： Kaspar HF

更新日期：1982-03-01 00:00:00

abstract：:A push-pull method, previously used in groundwater analyses, was successfully adapted for measuring sulfide turnover rates in situ at different depths in the meromictic Lake Cadagno. In the layer of phototrophic bacteria at about 12 m in depth net sulfide consumption was observed during the day, indicating active bact...

journal_title：Applied and environmental microbiology

authors： Lüthy L,Fritz M,Bachofen R

更新日期：2000-02-01 00:00:00

abstract：:In the United States, the 2009 pandemic influenza A (H1N1) virus (pH1N1) infected almost 20% of the population and caused >200,000 hospitalizations and >10,000 deaths from April 2009 to April 2010. On 24 April 2009, the CDC posted interim guidance on infection control measures in health care settings explicitly for pH...

journal_title：Applied and environmental microbiology

authors： Coulliette AD,Perry KA,Edwards JR,Noble-Wang JA

更新日期：2013-04-01 00:00:00

abstract：:Staphylococcus aureus is a gram-positive pathogen that causes a variety of diseases, including bovine mastitis, which has severe economic consequences. Standard antibiotic treatment results in selection of resistant strains, leading to a need for alternative treatments, such as bacteriophage therapy. Forty-nine S. aur...

journal_title：Applied and environmental microbiology

authors： Synnott AJ,Kuang Y,Kurimoto M,Yamamichi K,Iwano H,Tanji Y

更新日期：2009-07-01 00:00:00

abstract：:The susceptibility of a strain of Legionella pneumophila to disinfection by an organic halamine, free chlorine, and a mixture of the organic halamine and free chlorine was assessed. The organic halamine was found to have superior stability in solution and to exhibit adequate disinfectant potential over a period of 1 m...

journal_title：Applied and environmental microbiology

authors： Swango LJ,Wilt GR,Killen AD,Williams DE,Worley SD

更新日期：1987-12-01 00:00:00

abstract：:A xylose reductase (XR) gene was identified from the Neurospora crassa whole-genome sequence, expressed heterologously in Escherichia coli, and purified as a His6-tagged fusion in high yield. This enzyme is one of the most active XRs thus far characterized and may be used for the in vitro production of xylitol. ...

journal_title：Applied and environmental microbiology

authors： Woodyer R,Simurdiak M,van der Donk WA,Zhao H

更新日期：2005-03-01 00:00:00

abstract：:There is a wealth of evidence indicating that mobile genetic elements can spread in natural microbial communities. However, little is known regarding the fraction of the community that actually engages in this behavior. Here we report on a new approach to quantify the fraction of a bacterial community that is able to ...

journal_title：Applied and environmental microbiology

authors： Musovic S,Dechesne A,Sørensen J,Smets BF

更新日期：2010-07-01 00:00:00

abstract：:Short-chain fatty acid (SCFA) biosynthesis is pertinent to production of biofuels, industrial compounds, and pharmaceuticals from renewable resources. To expand on Escherichia coli SCFA products, we previously implemented a coenzyme A (CoA)-dependent pathway that condenses acetyl-CoA to a diverse group of short-chain ...

journal_title：Applied and environmental microbiology

authors： McMahon MD,Prather KL

更新日期：2014-02-01 00:00:00

abstract：:In this report, we describe the identification of functions that promote genomic recombination of linear DNA introduced into Pseudomonas cells by electroporation. The genes encoding these functions were identified in Pseudomonas syringae pv. syringae B728a based on similarity to the lambda Red Exo/Beta and RecET prote...

journal_title：Applied and environmental microbiology

authors： Swingle B,Bao Z,Markel E,Chambers A,Cartinhour S

更新日期：2010-08-01 00:00:00

abstract：:The phenylurea herbicide diuron [N-(3,4-dichlorophenyl)-N,N-dimethylurea] is widely used in a broad range of herbicide formulations, and consequently, it is frequently detected as a major water contaminant in areas where there is extensive use. We constructed a linuron [N-(3,4-dichlorophenyl)-N-methoxy-N-methylurea]- ...

journal_title：Applied and environmental microbiology

authors： Sørensen SR,Albers CN,Aamand J

更新日期：2008-04-01 00:00:00

abstract：:Fully grown broth cultures of various fast- and slow-growing rhizobia were deliberately diluted with various diluents before their aseptic incorporation into autoclaved peat in polypropylene bags (aseptic method) or mixed with the peat autoclaved in trays (tray method). In a factorial experiment with the aseptic metho...

journal_title：Applied and environmental microbiology

authors： Somasegaran P

更新日期：1985-08-01 00:00:00

abstract：:Using bacteria to transform reactive corrosion products into stable compounds represents an alternative to traditional methods employed in iron conservation. Two environmental Aeromonas strains (CA23 and CU5) were used to transform ferric iron corrosion products (goethite and lepidocrocite) into stable ferrous iron-be...

journal_title：Applied and environmental microbiology

authors： Kooli WM,Junier T,Shakya M,Monachon M,Davenport KW,Vaideeswaran K,Vernudachi A,Marozau I,Monrouzeau T,Gleasner CD,McMurry K,Lienhard R,Rufener L,Perret JL,Sereda O,Chain PS,Joseph E,Junier P

更新日期：2019-01-23 00:00:00

abstract：:Lysostaphin (Lst) is a potent bacteriolytic enzyme that kills Staphylococcus aureus, a common bacterial pathogen of humans and animals. With high activity against both planktonic cells and biofilms, Lst has the potential to be used in industrial products, such as commercial cleansers, for decontamination. However, Lst...

journal_title：Applied and environmental microbiology

authors： Wu X,Kwon SJ,Kim D,Zha J,Mora-Pale M,Dordick JS

更新日期：2018-07-02 00:00:00

abstract：:Infections caused by Toxoplasma gondii are widely prevalent in animals and humans throughout the world. In the United States, an estimated 23% of adolescents and adults have laboratory evidence of T. gondii infection. T. gondii has been identified as a major opportunistic pathogen in immunocompromised individuals, in ...

journal_title：Applied and environmental microbiology

authors： Schwab KJ,McDevitt JJ

更新日期：2003-10-01 00:00:00

abstract：:Clostridium botulinum 62A and ATCC 25763 spores required heat activation for maximum colony formation when plated on reinforced clostridial agar (BBL Microbiology Systems) but not when plated on botulinum assay medium. Spores from strains B-aphis and 53B did not exhibit heat activation when plated on either medium. ...

journal_title：Applied and environmental microbiology

authors： Montville TJ

更新日期：1981-10-01 00:00:00

abstract：:Water resources contaminated with wastewater are an important source for the dissemination of enteric viruses with an impact on the health of the population. The aim of the study was to assess the viral contamination of freshwater from a dam in Argentina by using infectious enterovirus detection, viral RNA amplificati...

journal_title：Applied and environmental microbiology

authors： Masachessi G,Pisano MB,Prez VE,Martínez LC,Michelena JF,Martínez-Wassaf M,Giordano MO,Isa MB,Pavan JV,Welter A,Nates SV,Ré V

更新日期：2018-02-14 00:00:00

abstract：:Malonyl coenzyme A (malonyl-CoA) and methylmalonyl-CoA are the most common extender units for the biosynthesis of fatty acids and polyketides in Streptomyces, an industrially important producer of polyketides. Carboxylation of acetyl- and propionyl-CoAs is an essential source of malonyl- and methylmalonyl-CoAs; theref...

journal_title：Applied and environmental microbiology

authors： Lyu M,Cheng Y,Han X,Wen Y,Song Y,Li J,Chen Z

更新日期：2020-06-02 00:00:00

abstract：:Enterohemorrhagic Escherichia coli (EHEC) is a significant zoonotic pathogen causing severe disease associated with watery and bloody diarrhea, hemorrhagic colitis, and the hemolytic-uremic syndrome (HUS) in humans. Infections are frequently associated with contact with EHEC-contaminated ruminant feces. Both natural a...

journal_title：Applied and environmental microbiology

authors： Joris MA,Vanrompay D,Verstraete K,De Reu K,De Zutter L,Cox E

更新日期：2013-06-01 00:00:00