Abstract:
:The acetylene block technique was used to assay denitrification in undisturbed sediment cores of an intertidal mud flat. Nitrogen loss due to this process was estimated at 1 to 41 kg of N/hectare (ha) per year. Anaerobic nitrate-saturated slurry of the same sediment had a denitrification capacity of 1,026 kg of N/ha per year. The acetylene block technique failed at low nitrate concentrations, so that denitrification at average in situ nitrate concentrations could not be determined. Denitrification followed zero-order kinetics at nitrate concentrations high enough to allow successful blockage of N(2)O reduction. Thus, an estimate of in situ rates based on kinetic parameters and in situ nitrate concentrations was impossible. No denitrification was observed in a slurry of the top 1.5 cm under aerated conditions and nitrate saturation. In undisturbed sediment, significant denitrification occurred in few discrete sites within a matrix of undetectable or low activity. Despite numerous errors contributing to the uncertainty of the estimate of in situ rates, the result obtained by this method was considered more valuable than the determination of denitrification capacities. Methods which include severe changes of physical and chemical parameters may frequently lead to overestimates of denitrification rates.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Kaspar HFdoi
10.1128/AEM.43.3.522-527.1982keywords:
subject
Has Abstractpub_date
1982-03-01 00:00:00pages
522-7issue
3eissn
0099-2240issn
1098-5336journal_volume
43pub_type
杂志文章abstract::Cyanobacterium Plectonema boryanum IU 594 and cyanophage LPP-1 were used as indicator organisms in a bioassay of 16 pesticides. Experiments such as spot tests, disk assays, growth curves, and one-step growth experiments were used to examine the effects of pesticides on the host and virus. Also, experiments were done i...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.47.5.910-914.1984
更新日期:1984-05-01 00:00:00
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doi:10.1128/AEM.02943-10
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doi:10.1128/aem.69.5.2624-2630.2003
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.41.2.511-517.1981
更新日期:1981-02-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/AEM.51.3.521-523.1986
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pub_type: 杂志文章
doi:10.1128/AEM.51.3.647-651.1986
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pub_type: 杂志文章
doi:10.1128/AEM.60.4.1093-1100.1994
更新日期:1994-04-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.8.2531-2537.1993
更新日期:1993-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.65.9.3880-3887.1999
更新日期:1999-09-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.8.2420-2425.1992
更新日期:1992-08-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.5.1732-1738.1997
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.57.11.3150-3155.1991
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更新日期:2003-10-01 00:00:00
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更新日期:2007-12-01 00:00:00
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.57.10.2891-2895.1991
更新日期:1991-10-01 00:00:00
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更新日期:2007-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/aem.66.6.2641-2646.2000
更新日期:2000-06-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:2014-05-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.12.4993-4995.1997
更新日期:1997-12-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.48.6.1129-1133.1984
更新日期:1984-12-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.7.4043-4048.2003
更新日期:2003-07-01 00:00:00
abstract::Chlamydiae are important pathogens of humans and animals but diagnosis of chlamydial infections is still hampered by inadequate detection methods. Fluorescence in situ hybridization (FISH) using rRNA-targeted oligonucleotide probes is widely used for the investigation of uncultured bacteria in complex microbial commun...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.8.4081-4089.2002
更新日期:2002-08-01 00:00:00