A simple, efficient method for the separation of humic substances and DNA from environmental samples.

abstract：:Nested PCR with three highly degenerate primers was used for amplification and identification of DNA polymerase (pol) genes from viruses which infect three genera of microalgae. Group-specific primers (AVS1 and AVS2) were designed on the basis of inferred amino acid sequences unique to the DNA pol genes of viruses (PB...

journal_title：Applied and environmental microbiology

authors： Chen F,Suttle CA

更新日期：1995-04-01 00:00:00

abstract：:Escherichia coli, commonly used in chemotaxis studies, is attracted mostly by amino acids, sugars, and peptides. We envisioned modifying the chemotaxis specificity of E. coli by expressing heterologous chemoreceptors from Pseudomonas putida enabling attraction either to toluene or benzoate. The mcpT gene encoding the ...

journal_title：Applied and environmental microbiology

authors： Roggo C,Clerc EE,Hadadi N,Carraro N,Stocker R,van der Meer JR

更新日期：2018-08-31 00:00:00

abstract：:A soil-inhabiting Fusarium proliferatum strain was capable of transforming or degrading nonlabeled and (sup14)C-labeled industrial, natural, and synthetic lignin. The mineralization rate per day (expressed as the percentage of added radioactivity recovered as to (sup14)CO(inf2)) was maximal during primary metabolism. ...

journal_title：Applied and environmental microbiology

authors： Regalado V,Rodriguez A,Perestelo F,Carnicero A,De La Fuente G,Falcon MA

更新日期：1997-09-01 00:00:00

abstract：:The pathways responsible for cell wall polysaccharide biosynthesis are vital in eukaryotic microorganisms. The corresponding synthases are potential targets of inhibitors such as fungicides. Despite their fundamental and economical importance, most polysaccharide synthases are not well characterized, and their molecul...

journal_title：Applied and environmental microbiology

authors： Briolay A,Bouzenzana J,Guichardant M,Deshayes C,Sindt N,Bessueille L,Bulone V

更新日期：2009-04-01 00:00:00

abstract：:The Sinorhizobium meliloti ORFeome project cloned 6,314 open reading frames (ORFs) into a modified Gateway entry vector system from which the ORFs could be transferred to destination vectors in vivo via bacterial conjugation. In this work, a reporter gene destination vector, pMK2030, was constructed and used to genera...

journal_title：Applied and environmental microbiology

authors： Humann JL,Schroeder BK,Mortimer MW,House BL,Yurgel SN,Maloney SC,Ward KL,Fallquist HM,Ziemkiewicz HT,Kahn ML

更新日期：2008-11-01 00:00:00

abstract：:Clostridium acetobutylicum is not able to grow on glycerol as the sole carbon source since it cannot reoxidize the excess of NADH generated by glycerol catabolism. Nevertheless, when the pSPD5 plasmid, carrying the NADH-consuming 1,3-propanediol pathway from C. butyricum VPI 3266, was introduced into C. acetobutylicum...

journal_title：Applied and environmental microbiology

authors： González-Pajuelo M,Meynial-Salles I,Mendes F,Soucaille P,Vasconcelos I

更新日期：2006-01-01 00:00:00

abstract：:Gene clusters for biosynthesis of the fungal polyketides hypothemycin and radicicol from Hypomyces subiculosus and Pochonia chlamydosporia, respectively, were sequenced. Both clusters encode a reducing polyketide synthase (PKS) and a nonreducing PKS like those in the zearalenone cluster of Gibberella zeae, plus enzyme...

journal_title：Applied and environmental microbiology

authors： Reeves CD,Hu Z,Reid R,Kealey JT

更新日期：2008-08-01 00:00:00

abstract：:Effects of the sesquiterpene farnesol (3,7,11-trimethyl-2,6,10-dodecatrien-1-ol) and the sesquiterpene derivative geosmin (1,10-trans-dimethyl-trans-9-decalol) were investigated in a geosmin-producing actinomycete, Streptomyces tendae. Exposure to 300 microM farnesol reduced biomass (fresh matter) accumulation by 97% ...

journal_title：Applied and environmental microbiology

authors： Dionigi CP,Millie DF,Johnsen PB

更新日期：1991-12-01 00:00:00

abstract：:Pseudomonas aureofaciens PGS12 produces three phenazine antibiotics, in addition to siderophores, hydrogen cyanide, pyrrolnitrin, and indoleacetic acid. Tn5-259.7 transposon mutagenesis was carried out to identify and clone a chromosomal locus involved in phenazine biosynthesis. Three classes of mutants were obtained:...

journal_title：Applied and environmental microbiology

authors： Georgakopoulos DG,Hendson M,Panopoulos NJ,Schroth MN

更新日期：1994-08-01 00:00:00

abstract：:An experiment was conducted with subsurface sediments from Oak Ridge National Laboratory to determine the potential for reduction of U(VI) under sulfate-reducing conditions with either ethanol or acetate as the electron donor. The results showed extensive U(VI) reduction in sediments supplied with either electron dono...

journal_title：Applied and environmental microbiology

authors： Converse BJ,Wu T,Findlay RH,Roden EE

更新日期：2013-07-01 00:00:00

abstract：:Protoplasts of Claviceps purpurea were prepared by treatment of mycelium with a lytic mixture of snail gut enzyme and cellulase from Trichoderma viride. Such protoplasts could be efficiently lysed by Triton X-100 treatment at high osmotic pressure without Ca or Mg, allowing the release of intact vacuoles in high yield...

journal_title：Applied and environmental microbiology

authors： Keller U,Madry N,Kleinkauf H,Glund K

更新日期：1984-04-01 00:00:00

abstract：:Escherichia coli isolates from rectal swabs from 62 chickens and stools from 42 children living in close contact with chickens on the same farms in Kiambu district, Kenya, were compared for their genetic relatedness. Antibiotic susceptibility profiles broadly categorized isolates from the children and from the chicken...

journal_title：Applied and environmental microbiology

authors： Kariuki S,Gilks C,Kimari J,Obanda A,Muyodi J,Waiyaki P,Hart CA

更新日期：1999-02-01 00:00:00

abstract：:Pseudomonas sp. strain ADP uses the herbicide atrazine as the sole nitrogen source. We have devised a simple atrazine degradation assay to determine the effect of other nitrogen sources on the atrazine degradation pathway. The atrazine degradation rate was greatly decreased in cells grown on nitrogen sources that supp...

journal_title：Applied and environmental microbiology

authors： García-González V,Govantes F,Shaw LJ,Burns RG,Santero E

更新日期：2003-12-01 00:00:00

abstract：:Whole cells of the parent strain of Beijerinckia, grown with succinate and biphenyl, oxidized dibenzo-p-dioxin and several chlorinated dioxins. The rate of oxidation of the chlorinated dibenzo-p-dioxins decreased with an increasing degree of chlorine substitution. A mutant strain (B8/36) of Beijerinckia oxidized diben...

journal_title：Applied and environmental microbiology

authors： Klecka GM,Gibson DT

更新日期：1980-02-01 00:00:00

abstract：:Geographical and seasonal variation in the incidence and prevalence of Campylobacter jejuni and C. coli in housed broiler flocks reared in Great Britain in 2004 to 2006 was investigated in this study. Ceca (30) from 797 flocks, not subject to prior partial depopulation and reared on 211 farms, were examined individual...

journal_title：Applied and environmental microbiology

authors： Jorgensen F,Ellis-Iversen J,Rushton S,Bull SA,Harris SA,Bryan SJ,Gonzalez A,Humphrey TJ

更新日期：2011-06-01 00:00:00

abstract：:Yarrowia lipolytica produces brown extracellular pigments that correlate with tyrosine catabolism. During tyrosine depletion, the yeast accumulated homogentisic acid, p-hydroxyphenylethanol, and p-hydroxyphenylacetic acid in the medium. Homogentisic acid accumulated under all aeration conditions tested, but its concen...

journal_title：Applied and environmental microbiology

authors： Carreira A,Ferreira LM,Loureiro V

更新日期：2001-08-01 00:00:00

abstract：:Using a chemically defined medium without L-alanine, Lactobacillus johnsonii was demonstrated to be strictly auxotrophic for that amino acid. A comparative genetic analysis showed that all known genes involved in L-alanine biosynthesis are absent from the genome of L. johnsonii. This auxotrophy was complemented by het...

journal_title：Applied and environmental microbiology

authors： van der Kaaij H,Desiere F,Mollet B,Germond JE

更新日期：2004-03-01 00:00:00

abstract：:The photosynthetic bacterium Rhodobacter sphaeroides is capable of producing H2 via nitrogenase when grown photoheterotrophically in the absence of N2. By using 14C-labeled malate, it was found that greater than 95% of this substrate was catabolized completely to CO2 during H2 production. About 60% of this catabolism ...

journal_title：Applied and environmental microbiology

authors： Macler BA,Bassham JA

更新日期：1988-11-01 00:00:00

abstract：:The fate of 14C-labeled 2,4,6-trinitrotoluene (TNT) in an activated-sludge system was investigated. No [14C]TNT could be detected in the contents of an aerated reactor after 3 to 5 days of incubation. No significant 14CO2 was formed, and the radioactivity was about equally divided between the floc and the supernatant....

journal_title：Applied and environmental microbiology

authors： Carpenter DF,McCormick NG,Cornell JH,Kaplan AM

更新日期：1978-05-01 00:00:00

abstract：:An anaerobic diplococcoid bacterium able to degrade hydrolyzable tannins was isolated from the ruminal fluid of a goat fed desmodium (Desmodium ovalifolium), a tropical legume which contains levels as high as 17% condensed tannins. This strain grew under anaerobic conditions in the presence of up to 30 g of tannic aci...

journal_title：Applied and environmental microbiology

authors： Nelson KE,Pell AN,Schofield P,Zinder S

更新日期：1995-09-01 00:00:00

abstract：UNLABELLED:Clostridium perfringens type A is a common source of foodborne illness (FBI) in humans. Vegetative cells sporulate in the small intestinal tract and produce the major pathogenic factor C. perfringens enterotoxin. Although sporulation plays a critical role in the pathogenesis of FBI, the mechanisms inducing s...

journal_title：Applied and environmental microbiology

authors： Yasugi M,Okuzaki D,Kuwana R,Takamatsu H,Fujita M,Sarker MR,Miyake M

更新日期：2016-05-02 00:00:00

abstract：:Fusarium species from agricultural crops have been well studied with respect to toxin production and genetic diversity, while similar studies of communities from nonagricultural plants are much more limited. We examined 72 Fusarium isolates from a native North American tallgrass prairie and found that Gibberella inter...

journal_title：Applied and environmental microbiology

authors： Leslie JF,Zeller KA,Logrieco A,Mulè G,Moretti A,Ritieni A

更新日期：2004-04-01 00:00:00

abstract：:Saturation point mutagenesis was carried out at position 479 in the polyhydroxyalkanoate (PHA) synthase from Chromobacterium sp. strain USM2 (PhaC(Cs)) with specificities for short-chain-length (SCL) [(R)-3-hydroxybutyrate (3HB) and (R)-3-hydroxyvalerate (3HV)] and medium-chain-length (MCL) [(R)-3-hydroxyhexanoate (3H...

journal_title：Applied and environmental microbiology

authors： Chuah JA,Tomizawa S,Yamada M,Tsuge T,Doi Y,Sudesh K,Numata K

更新日期：2013-06-01 00:00:00

abstract：:We constructed a 7.9-kilobase-pair recombinant shuttle plasmid, designated pHR106, by combining desired segments of three plasmids: an Escherichia coli plasmid (pSL100) which provides a multiple cloning site, a Clostridium perfringens plasmid (pJU122) which provides a clostridial origin of replication, and an E. coli ...

journal_title：Applied and environmental microbiology

authors： Roberts I,Holmes WM,Hylemon PB

更新日期：1988-01-01 00:00:00

abstract：:The susceptibility of the Iberian ibex (Capra pyrenaica) to Mycoplasma conjunctivae ocular infection and the changes in their interaction over time were studied in terms of clinical outcome, molecular detection, and IgG immune response in a captive population that underwent a severe infectious keratoconjunctivitis (IK...

journal_title：Applied and environmental microbiology

authors： Fernández-Aguilar X,Cabezón O,Granados JE,Frey J,Serrano E,Velarde R,Cano-Manuel FJ,Mentaberre G,Ráez-Bravo A,Fandos P,López-Olvera JR

更新日期：2017-07-17 00:00:00

abstract：:A method was developed to study the biodegradation and oxidative biodehalogenation of fluorinated phenols by 19F nuclear magnetic resonance (NMR). Characterization of the 19F NMR spectra of metabolite profiles of a series of fluorophenols, converted by purified phenol hydroxylase, catechol 1,2-dioxygenase, and/or by t...

journal_title：Applied and environmental microbiology

authors： Boersma MG,Dinarieva TY,Middelhoven WJ,van Berkel WJ,Doran J,Vervoort J,Rietjens IM

更新日期：1998-04-01 00:00:00

abstract：:Upflow, anaerobic, fixed-bed, activated charcoal biotreatment columns capable of operating at free cyanide concentrations of greater than 100 mg liter-1 with a hydraulic retention time of less than 48 h were developed. Methanogenesis was maintained under a variety of feed medium conditions which included ethanol, phen...

journal_title：Applied and environmental microbiology

authors： Fallon RD,Cooper DA,Speece R,Henson M

更新日期：1991-06-01 00:00:00

abstract：:A novel sulfate-reducing bacterium isolated from fuel-contaminated subsurface soil, strain PRTOL1, mineralizes toluene as the sole electron donor and carbon source under strictly anaerobic conditions. The mineralization of 80% of toluene carbon to CO2 was demonstrated in experiments with [ring-U-14C]toluene; 15% of to...

journal_title：Applied and environmental microbiology

authors： Beller HR,Spormann AM,Sharma PK,Cole JR,Reinhard M

更新日期：1996-04-01 00:00:00

abstract：:Disulfide bonds are important for the correct folding, structural integrity, and activity of many biotechnologically relevant proteins. For synthesis and subsequent secretion of these proteins in bacteria, such as the well-known "cell factory" Bacillus subtilis, it is often the correct formation of disulfide bonds tha...

journal_title：Applied and environmental microbiology

authors： Kouwen TR,Dubois JY,Freudl R,Quax WJ,van Dijl JM

更新日期：2008-12-01 00:00:00

abstract：:Proteorhodopsin (PR), a photoactive proton pump containing retinal, is present in approximately half of all bacteria in the ocean, but its physiological role is still unclear, since very few strains carrying the PR gene have been cultured. The aim of this work was to characterize PR diversity in a North Sea water samp...

journal_title：Applied and environmental microbiology

authors： Riedel T,Tomasch J,Buchholz I,Jacobs J,Kollenberg M,Gerdts G,Wichels A,Brinkhoff T,Cypionka H,Wagner-Döbler I

更新日期：2010-05-01 00:00:00