Modulation of thiol-disulfide oxidoreductases for increased production of disulfide-bond-containing proteins in Bacillus subtilis.

abstract：:The isophthalate (IPA) degradation gene cluster (iphACBDR) responsible for the conversion of IPA into protocatechuate (PCA) was isolated from Comamonas sp. strain E6, which utilizes phthalate isomers as sole carbon and energy sources via the PCA 4,5-cleavage pathway. Based on amino acid sequence similarity, the iphA, ...

journal_title：Applied and environmental microbiology

authors： Fukuhara Y,Inakazu K,Kodama N,Kamimura N,Kasai D,Katayama Y,Fukuda M,Masai E

更新日期：2010-01-01 00:00:00

abstract：:Aerobic biotransformation of the diaryl ethers 2,7-dichlorodibenzo-p-dioxin and 1,2,3,4-tetrachlorodibenzo-p-dioxin by the dibenzo-p-dioxin-utilizing strain Sphingomonas wittichii RW1, producing corresponding metabolites, was demonstrated for the first time. Our strain transformed 2,7-dichlorodibenzo-p-dioxin, yieldin...

journal_title：Applied and environmental microbiology

authors： Hong HB,Chang YS,Nam IH,Fortnagel P,Schmidt S

更新日期：2002-05-01 00:00:00

abstract：UNLABELLED:The l-isoaspartyl protein carboxyl methyltransferase (PCM) repairs protein damage resulting from spontaneous conversion of aspartyl or asparaginyl residues to isoaspartate and increases long-term stationary-phase survival of Escherichia coli under stress. In the course of studies intended to examine PCM func...

journal_title：Applied and environmental microbiology

authors： VandenBerg KE,Ahn S,Visick JE

更新日期：2016-08-15 00:00:00

abstract：:The assembly of six genes that encode enzymes from glutamate-fermenting bacteria converted Escherichia coli into a glutaconate producer when grown anaerobically on a complex medium. The new anaerobic pathway starts with 2-oxoglutarate from general metabolism and proceeds via (R)-2-hydroxyglutarate, (R)-2-hydroxyglutar...

journal_title：Applied and environmental microbiology

authors： Djurdjevic I,Zelder O,Buckel W

更新日期：2011-01-01 00:00:00

abstract：:A set of freshwater mesocosms (1.7 m3 each) was inoculated with large amounts of Escherichia coli, Pseudomonas putida, and their culture medium to substantially disturb the natural microbial community. To monitor microbial community dynamics, low-molecular-weight RNA (5S rRNA and tRNA) obtained directly from bacteriop...

journal_title：Applied and environmental microbiology

authors： Höfle MG

更新日期：1992-10-01 00:00:00

abstract：:The vacuum residue fraction of heavy crudes contributes to the viscosity of these oils. Specific microbial cleavage of C-S bonds in alkylsulfide bridges that form linkages in this fraction may result in dramatic viscosity reduction. To date, no bacterial strains have been shown conclusively to cleave C-S bonds within ...

journal_title：Applied and environmental microbiology

authors： Van Hamme JD,Fedorak PM,Foght JM,Gray MR,Dettman HD

更新日期：2004-03-01 00:00:00

abstract：:The intracellular pathogens Legionella micdadei and Legionella pneumophila are the two most common Legionella species that cause Legionnaires' disease. Intracellular replication within pulmonary cells is the hallmark of Legionnaires' disease. In the environment, legionellae are parasites of protozoans, and intracellul...

journal_title：Applied and environmental microbiology

authors： Abu Kwaik Y,Venkataraman C,Harb OS,Gao LY

更新日期：1998-09-01 00:00:00

abstract：:GELRITE, a new gelling agent with good thermal stability and clarity, was evaluated in media for culturing selected thermophilic microorganisms. It was also evaluated for performing counts of thermophilic bacteria from soil samples. In most cases, GELRITE was shown to be superior to agar for these applications. ...

journal_title：Applied and environmental microbiology

authors： Lin CC,Casida LE

更新日期：1984-02-01 00:00:00

abstract：:Methane (CH(4)) flux to the atmosphere is mitigated via microbial CH(4) oxidation in sediments and water. As arctic temperatures increase, understanding the effects of temperature on the activity and identity of methanotrophs in arctic lake sediments is important to predicting future CH(4) emissions. We used DNA-based...

journal_title：Applied and environmental microbiology

authors： He R,Wooller MJ,Pohlman JW,Quensen J,Tiedje JM,Leigh MB

更新日期：2012-07-01 00:00:00

abstract：:Indoxyl-beta-D-glucuronide (indoxyl) was evaluated as a specific chromogen for detection of Escherichia coli by the membrane filter method. In all, 413 colonies were tested from the indoxyl-supplemented media, yielding 93.3% confirmation, as E. coli. Compared with the indoxyl medium, other media gave either much lower...

journal_title：Applied and environmental microbiology

authors： Haines JR,Covert TC,Rankin CC

更新日期：1993-08-01 00:00:00

abstract：:Sclerotinia sclerotiorum acidifies its ambient environment by producing oxalic acid. This production of oxalic acid during plant infection has been implicated as a primary determinant of pathogenicity in this and other phytopathogenic fungi. We found that ambient pH conditions affect multiple processes in S. sclerotio...

journal_title：Applied and environmental microbiology

authors： Rollins JA,Dickman MB

更新日期：2001-01-01 00:00:00

abstract：:The function of small secreted proteins (SSPs) in saprotrophic fungi is, for the most part, unknown. The white-rot mushroom Pleurotus ostreatus produces considerable amounts of SSPs at the onset of secondary metabolism, during colony development, and in response to chemical compounds such as 5-hydroxymethylfurfural an...

journal_title：Applied and environmental microbiology

authors： Feldman D,Amedi N,Carmeli S,Yarden O,Hadar Y

更新日期：2019-07-18 00:00:00

abstract：:The use of bioluminescence as a sensitive marker for detection of Pseudomonas spp. in the rhizosphere was investigated. Continuous expression of the luxCDABE genes, required for bioluminescence, was not detectable in the rhizosphere. However, when either a naphthalene-inducible luxCDABE construct or a constitutive lux...

journal_title：Applied and environmental microbiology

authors： de Weger LA,Dunbar P,Mahafee WF,Lugtenberg BJ,Sayler GS

更新日期：1991-12-01 00:00:00

abstract：:Bacterial utilization of crude oil components, such as the n-alkanes, requires complex cell surface adaptation to allow adherence to oil. To better understand microbial cell surface adaptation to growth on crude oil, the cell surface characteristics of two Pseudomonas aeruginosa strains, U1 and U3, both isolated from ...

journal_title：Applied and environmental microbiology

authors： Norman RS,Frontera-Suau R,Morris PJ

更新日期：2002-10-01 00:00:00

abstract：:Recent isolation of Pseudomonas aeruginosa strains from the open ocean and subsequent pulsed-field gel electrophoresis analyses indicate that these strains have a unique genotype (N. H. Khan, Y. Ishii, N. Kimata-Kino, H. Esaki, T. Nishino, M. Nishimura, and K. Kogure, Microb. Ecol. 53:173-186, 2007). We hypothesized t...

journal_title：Applied and environmental microbiology

authors： Khan NH,Ahsan M,Yoshizawa S,Hosoya S,Yokota A,Kogure K

更新日期：2008-10-01 00:00:00

abstract：:Conditioned medium was obtained from suspension cultures of soybean (Glycine max L. Merrit) cells after incubating them for 4 to 8 days with rhizobia which were separated from the soybean cells by two dialysis bags, one within another. This conditioned medium from the plant cell side (PCM) of the two membranes was use...

journal_title：Applied and environmental microbiology

authors： Bednarski MA,Reporter M

更新日期：1978-07-01 00:00:00

abstract：:The utilization by Alcaligenes faecalis of electrodes as the electron donor for denitrification was investigated in this study. The denitrification rate of A. faecalis with a poised potential was greatly enhanced compared with that of the controls without poised potentials. For nitrate reduction, although A. faecalis ...

journal_title：Applied and environmental microbiology

authors： Wang X,Yu P,Zeng C,Ding H,Li Y,Wang C,Lu A

更新日期：2015-08-15 00:00:00

abstract：:The well-established killing of bacteria by copper surfaces, also called contact killing, is currently believed to be a combined effect of bacterial contact with the copper surface and the dissolution of copper, resulting in lethal bacterial damage. Iron can similarly be released in ionic form from iron surfaces and w...

journal_title：Applied and environmental microbiology

authors： Mathews S,Kumar R,Solioz M

更新日期：2015-09-01 00:00:00

abstract：:Ethane oxidation was studied in ethane-grown resting cells (mycelia) of an Acremonium sp. and in cell-free preparations of such mycelia. From resting cell experiments evidence was found for a pathway of ethane oxidation via ethanol, acetaldehyde, and acetic acid. In vitro studies indicated that ethane-oxidizing activi...

journal_title：Applied and environmental microbiology

authors： Davies JS,Wellman AM,Zajic JE

更新日期：1976-07-01 00:00:00

abstract：:Suspension of adenovirus type 5 in 2.0 ml of cell culture fluid at 37 degrees C were subjected to smoke from four cigarettes over a 4-h period. The cigarettes were smoked in a normal manner, and the inhaled smoke was exhaled through glass tubing into the virus-containing fluid. The virus suspensions were then titrated...

journal_title：Applied and environmental microbiology

authors： Bardell D

更新日期：1978-11-01 00:00:00

abstract：:The fungus Fusarium oxysporum produced a D-amino acid oxidase (EC 1. 4.3.3) in a medium containing glucose as the carbon and energy source and ammonium sulfate as the nitrogen source. The specific D-amino acid oxidase activity was increased up to 12.5-fold with various D-amino acids or their corresponding derivatives ...

journal_title：Applied and environmental microbiology

authors： Gabler M,Fischer L

更新日期：1999-08-01 00:00:00

abstract：:Gas chromatographic studies showed that nitrous oxide was produced in each instance when sterilized (autoclaved) soil was incubated after treatment with ammonium sulfate and inoculation with pure cultures of ammonia-oxidizing chemoautotrophic microorganisms (strains of Nitrosomonas, Nitrosospira, and Nitrosolobus). Pr...

journal_title：Applied and environmental microbiology

authors： Blackmer AM,Bremner JM,Schmidt EL

更新日期：1980-12-01 00:00:00

abstract：:Analysis of electron spin resonance spectra of 5-doxyl stearic acid in aqueous suspensions of Clostridium acetobutylicum ATCC 824 and the butanol-tolerant SA-2 derivative during a small-scale fermentation at three different butanol challenge levels indicated that the SA-2 strain is able to respond to the physical flui...

journal_title：Applied and environmental microbiology

authors： Baer SH,Bryant DL,Blaschek HP

更新日期：1989-10-01 00:00:00

abstract：:Downstream from the ptsHI operon of Lactobacillus sakei, the genes atkY and atkB, organized in an operon, were observed. The two putative proteins, AtkB and AtkY, show sequence similarity to the Enterococcus hirae copper P-type ATPase, responsible for copper efflux, and its negative regulator. Characterization of AtkB...

journal_title：Applied and environmental microbiology

authors： Stentz R,Loizel C,Malleret C,Zagorec M

更新日期：2000-10-01 00:00:00

abstract：:Rapid, high-throughput assays that detect and quantify botulinum neurotoxin (BoNT) activity in diverse matrices are required for environmental, clinical, pharmaceutical, and food testing. The current standard, the mouse bioassay, is sensitive but is low in throughput and precision. In this study, we present three bioc...

journal_title：Applied and environmental microbiology

authors： Dunning FM,Ruge DR,Piazza TM,Stanker LH,Zeytin FN,Tucker WC

更新日期：2012-11-01 00:00:00

abstract：:Two strains of 1,1,1-trichloroethane (TCA)-degrading bacteria, TA5 and TA27, were isolated from soil and identified as Mycobacterium spp. Strains TA5 and TA27 could degrade 25 and 75 mg. liter of TCA(-1) cometabolically in the presence of ethane as a carbon source, respectively. The compound 2,2,2-trichloroethanol was...

journal_title：Applied and environmental microbiology

authors： Yagi O,Hashimoto A,Iwasaki K,Nakajima M

更新日期：1999-10-01 00:00:00

abstract：:Due to the rapid expansion in microbial taxonomy, precise identification of common industrially and agriculturally relevant fungi such as Trichoderma species is challenging. In this study, we introduce the online multilocus identification system (MIST) for automated detection of 349 Trichoderma species based on a set ...

journal_title：Applied and environmental microbiology

authors： Dou K,Lu Z,Wu Q,Ni M,Yu C,Wang M,Li Y,Wang X,Xie H,Chen J,Zhang C

更新日期：2020-09-01 00:00:00

abstract：:Listeria monocytogenes is among the most important food-borne pathogens and is well adapted to persist in the environment. To gain insight into the genetic relatedness and potential virulence of L. monocytogenes strains causing central nervous system (CNS) infections, we used multilocus variable-number tandem-repeat a...

journal_title：Applied and environmental microbiology

authors： Balandyté L,Brodard I,Frey J,Oevermann A,Abril C

更新日期：2011-12-01 00:00:00

abstract：:We show that Rhodococcus chlorophenolicus PCP-I, a polychlorophenol degrader, also degrades various chlorine-substituted guaiacols (2-methoxyphenols) and syringols (2,6-dimethoxyphenols). The substrates investigated were tetrachloroguaiacol, 3,4,6- and 3,5,6-trichloroguaiacol, 3,5- and 3,6-dichloroguaiacol, trichloros...

journal_title：Applied and environmental microbiology

authors： Häggblom MM,Apajalahti JH,Salkinoja-Salonen MS

更新日期：1988-03-01 00:00:00

abstract：:Wolbachia, a common bacterial endosymbiont of insects, has been shown to protect its hosts against a wide range of pathogens. However, not all strains exert a protective effect on their host. Here we assess the effects of two divergent Wolbachia strains, wAlbB from Aedes albopictus and wMelPop from Drosophila melanoga...

journal_title：Applied and environmental microbiology

authors： Hughes GL,Vega-Rodriguez J,Xue P,Rasgon JL

更新日期：2012-03-01 00:00:00