Development of genetic tools for Lactobacillus sakei: disruption of the beta-galactosidase gene and use of lacZ as a reporter gene To study regulation of the putative copper ATPase, AtkB.

abstract：:Colonization of apple leaves by the yeastlike fungus Aureobasidium pullulans was followed quantitatively and spatially at a microscale level throughout two growing seasons. Ten field leaves were sampled on 11 dates in 2003 and 15 dates in 2004. Using an A. pullulans-specific fluorescence in situ hybridization probe an...

journal_title：Applied and environmental microbiology

authors： McGrath MJ,Andrews JH

更新日期：2006-09-01 00:00:00

abstract：:A bacterium capable of anaerobic growth via reductive dehalogenation of 2-chlorophenol was isolated from a culture enriched from sediment taken from a small stream near Lansing, Mich. The organism, designated strain 2CP-1, is a gram-negative rod ca. 3 by 0.5 micron in size and is a catalase-negative, oxidase-negative,...

journal_title：Applied and environmental microbiology

authors： Cole JR,Cascarelli AL,Mohn WW,Tiedje JM

更新日期：1994-10-01 00:00:00

abstract：:To study the impact of solar UV radiation (UVR) (280 to 400 nm) on the filamentous cyanobacterium Arthrospira (Spirulina) platensis, we examined the morphological changes and photosynthetic performance using an indoor-grown strain (which had not been exposed to sunlight for decades) and an outdoor-grown strain (which ...

journal_title：Applied and environmental microbiology

authors： Wu H,Gao K,Villafañe VE,Watanabe T,Helbling EW

更新日期：2005-09-01 00:00:00

abstract：:Intergeneric conjugal transfer of plasmid DNA from Escherichia coli to Streptomyces circumvents problems such as host-controlled restriction and instability of foreign DNA during the transformation of Streptomyces protoplasts. The anthracycline antibiotic-producing strains Streptomyces peucetius and Streptomyces sp. s...

journal_title：Applied and environmental microbiology

authors： Paranthaman S,Dharmalingam K

更新日期：2003-01-01 00:00:00

abstract：:The distribution of Borrelia burgdorferi sensu lato genospecies in questing Ixodes ricinus ticks from ecologically distinct habitats in Latvia was analyzed. A significant variation in the frequency of the genospecies across sites was observed, pointing to the importance of the host community in the ecology of Lyme bor...

journal_title：Applied and environmental microbiology

authors： Etti S,Hails R,Schäfer SM,De Michelis S,Sewell HS,Bormane A,Donaghy M,Kurtenbach K

更新日期：2003-05-01 00:00:00

abstract：:The changes in motility, chemotactic responsiveness, and flagellation of Rhizobium meliloti RMB7201, L5-30, and JJ1c10 were analyzed after transfer of the bacteria to buffer with no available C, N, or phosphate. Cells of these three strains remained viable for weeks after transfer to starvation buffer (SB) but lost al...

journal_title：Applied and environmental microbiology

authors： Wei X,Bauer WD

更新日期：1998-05-01 00:00:00

abstract：:Escherichia coli is a widely utilized indicator of the sanitary quality of bivalve molluscan shellfish sold for human consumption. However, it is now well documented that shellfish that meet the E. coli standards for human consumption may contain human enteric viruses that cause gastroenteritis and hepatitis. In this ...

journal_title：Applied and environmental microbiology

authors： Doré WJ,Henshilwood K,Lees DN

更新日期：2000-04-01 00:00:00

abstract：:Ursodeoxycholic acid dissolves cholesterol gallstones in humans. In the present study optimum conditions for ursodeoxycholic acid production by Fusarium equiseti M41 were studied. Resting mycelia of F. equiseti M41 showed maximum conversion at 28 degrees C, pH 8.0, and dissolved oxygen tension of higher than 60% satur...

journal_title：Applied and environmental microbiology

authors： Kulprecha S,Ueda T,Nihira T,Yoshida T,Taguchi H

更新日期：1985-02-01 00:00:00

abstract：:Accurate predictions of carbon and energy cycling rates in the environment depend on sampling frequencies and on the spatial variability associated with biological activities. We examined the variability associated with anaerobic biodegradation rates at two sites in an alluvial sand aquifer polluted by municipal landf...

journal_title：Applied and environmental microbiology

authors： Adrian NR,Robinson JA,Suflita JM

更新日期：1994-10-01 00:00:00

abstract：:Compared to freshwater and the open ocean, less is known about bacterioplankton community structure and spatiotemporal dynamics in estuaries, particularly those with long residence times. The Chesapeake Bay is the largest estuary in the United States, but despite its ecological and economic significance, little is kno...

journal_title：Applied and environmental microbiology

authors： Kan J,Suzuki MT,Wang K,Evans SE,Chen F

更新日期：2007-11-01 00:00:00

abstract：:Many marine fish harbor luminous bacteria as bioluminescent symbionts. Despite the diversity, abundance, and ecological importance of these fish and their apparent dependence on luminous bacteria for survival and reproduction, little is known about developmental and microbiological events surrounding the inception of ...

journal_title：Applied and environmental microbiology

authors： Dunlap PV,Davis KM,Tomiyama S,Fujino M,Fukui A

更新日期：2008-12-01 00:00:00

abstract：:Media used to determine the MICs of heavy metals for bacteria are unreliable because organic components in the media bind or chelate most of the metal being studied. To define specific metal activity in media and to maintain metal activity at a constant level, HEPES-MES [N-2-hydroxyethylpiperazine-N' -2-ethanesulfonic...

journal_title：Applied and environmental microbiology

authors： Angle JS,Chaney RL

更新日期：1989-08-01 00:00:00

abstract：:Poplar, a plant species frequently used for phytoremediation of groundwater contaminated with organic solvents, was inoculated with the endophyte Burkholderia cepacia VM1468. This strain, whose natural host is yellow lupine, contains the pTOM-Bu61 plasmid coding for constitutively expressed toluene degradation. Nonino...

journal_title：Applied and environmental microbiology

authors： Taghavi S,Barac T,Greenberg B,Borremans B,Vangronsveld J,van der Lelie D

更新日期：2005-12-01 00:00:00

abstract：:Crude extracts from 14 species of sulfate-reducing bacteria comprising the genera Desulfovibrio, Desulfotomaculum, Desulfobulbus, and Desulfosarcina and from three species of sulfide-oxidizing bacteria were tested in an enzyme-linked immunosorbent assay with polyclonal antisera to adenosine 5'-phosphosulfate reductase...

journal_title：Applied and environmental microbiology

authors： Odom JM,Jessie K,Knodel E,Emptage M

更新日期：1991-03-01 00:00:00

abstract：:The extremely thermophilic anaerobic archaeon strain B1001 was isolated from a hot-spring environment in Japan. The cells were irregular cocci, 0.5 to 1.0 micrometers in diameter. The new isolate grew at temperatures between 60 and 95 degrees C (optimum, 85 degrees C), from pH 5.0 to 9.0 (optimum, pH 7.0), and from 1....

journal_title：Applied and environmental microbiology

authors： Tachibana Y,Kuramura A,Shirasaka N,Suzuki Y,Yamamoto T,Fujiwara S,Takagi M,Imanaka T

更新日期：1999-05-01 00:00:00

abstract：:The Alvord Basin in southeast Oregon contains a variety of hydrothermal features which have never been microbiologically characterized. A sampling of Murky Pot (61 degrees C; pH 7.1) led to the isolation of a novel arsenic-metabolizing organism (YeAs) which produces an arsenic sulfide mineral known as beta-realgar, a ...

journal_title：Applied and environmental microbiology

authors： Ledbetter RN,Connon SA,Neal AL,Dohnalkova A,Magnuson TS

更新日期：2007-09-01 00:00:00

abstract：:The ability of the facultative photoheterotroph Rhodobacter sphaeroides to tolerate and reduce high levels of tellurite in addition to at least 10 other rare earth metal oxides and oxyanions has considerable potential for detoxification and bioremediation of contaminated environments. We report the identification and ...

journal_title：Applied and environmental microbiology

authors： O'Gara JP,Gomelsky M,Kaplan S

更新日期：1997-12-01 00:00:00

abstract：:In the entomopathogenic bacterium Xenorhabdus nematophila, cell-to-cell variation in the abundance of the Lrp transcription factor leads to virulence modulation; low Lrp levels are associated with a virulent phenotype and suppression of antimicrobial peptides (AMPs) in Manduca sexta insects, while cells that lack lrp ...

journal_title：Applied and environmental microbiology

authors： Casanova-Torres ÁM,Shokal U,Morag N,Eleftherianos I,Goodrich-Blair H

更新日期：2017-05-31 00:00:00

abstract：:Using a chemically defined medium without L-alanine, Lactobacillus johnsonii was demonstrated to be strictly auxotrophic for that amino acid. A comparative genetic analysis showed that all known genes involved in L-alanine biosynthesis are absent from the genome of L. johnsonii. This auxotrophy was complemented by het...

journal_title：Applied and environmental microbiology

authors： van der Kaaij H,Desiere F,Mollet B,Germond JE

更新日期：2004-03-01 00:00:00

abstract：:Plants are in constant contact with a community of soil biota that contains fungi ranging from pathogenic to symbiotic. A few studies have demonstrated a critical role of chemical communication in establishing highly specialized relationships, but the general role for root exudates in structuring the soil fungal commu...

journal_title：Applied and environmental microbiology

authors： Broeckling CD,Broz AK,Bergelson J,Manter DK,Vivanco JM

更新日期：2008-02-01 00:00:00

abstract：:The erythromycin resistance gene ermB has been found in a variety of gram-positive bacteria. This gene has also been found in Bacteroides species but only in six recently isolated strains; thus, the gene seems to have entered this genus only recently. One of the six Bacteroides ermB-containing isolates, WH207, could t...

journal_title：Applied and environmental microbiology

authors： Gupta A,Vlamakis H,Shoemaker N,Salyers AA

更新日期：2003-11-01 00:00:00

abstract：:We found that a robust energy taxis response mediated by the Aer receptor can sometimes mask chemotaxis mediated by other methyl-accepting chemotaxis proteins (MCPs) in Pseudomonas aeruginosa. We identified PA2652 as a chemoreceptor for malate by screening aer mcp double mutants by using swarm plate assays. ...

journal_title：Applied and environmental microbiology

authors： Alvarez-Ortega C,Harwood CS

更新日期：2007-12-01 00:00:00

abstract：:Commercial Atlantic blue crabs (Callinectes sapidus) were exposed to 2.0x10(4) infectious waterborne oocysts of Cryptosporidium parvum. The study demonstrated that blue crabs can transfer C. parvum oocysts to persons involved in handling or preparing crabs and that they may contaminate other surfaces or products durin...

journal_title：Applied and environmental microbiology

authors： Graczyk TK,McOliver C,Silbergeld EK,Tamang L,Roberts JD

更新日期：2007-06-01 00:00:00

abstract：:Pili (fimbriae) were observed on cells of each of the five strains of Bradyrhizobium japonicum and the one strain of Rhizobium trifolii examined. Pili on B. japonicum were about 4 nm in diameter and polarly expressed. Piliated cells were estimated by transmission electron microscopy and hydrophobic attachment to polys...

journal_title：Applied and environmental microbiology

authors： Vesper SJ,Bauer WD

更新日期：1986-07-01 00:00:00

abstract：:A novel thermostable alcohol dehydrogenase (ADH) showing activity toward aromatic secondary alcohols was identified from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1 (TkADH). The gene, tk0845, which encodes an aldo-keto reductase, was heterologously expressed in Escherichia coli. The enzyme was found ...

journal_title：Applied and environmental microbiology

authors： Wu X,Zhang C,Orita I,Imanaka T,Fukui T,Xing XH

更新日期：2013-04-01 00:00:00

abstract：:Temperature and relative humidity are major factors determining virus inactivation in the environment. This article reviews inactivation data regarding coronaviruses on surfaces and in liquids from published studies and develops secondary models to predict coronaviruses inactivation as a function of temperature and re...

journal_title：Applied and environmental microbiology

authors： Guillier L,Martin-Latil S,Chaix E,Thébault A,Pavio N,Le Poder S,Batéjat C,Biot F,Koch L,Schaffner DW,Sanaa M,Covid-19 Emergency Collective Expert Appraisal Group.

更新日期：2020-09-01 00:00:00

abstract：:We studied injury of Escherichia coli O157:H7 cells in 11 food items during freeze storage and methods of isolating freeze-injured E. coli O157:H7 cells from foods. Food samples inoculated with E. coli O157:H7 were stored for 16 weeks at -20 degrees C in a freezer. Noninjured and injured cells were counted by using tr...

journal_title：Applied and environmental microbiology

authors： Hara-Kudo Y,Ikedo M,Kodaka H,Nakagawa H,Goto K,Masuda T,Konuma H,Kojima T,Kumagai S

更新日期：2000-07-01 00:00:00

abstract：:Escherichia coli, a commensal bacterium from the intestinal tracts of humans and vertebrate animals, has been used as one of two bacterial indicators of fecal contamination, along with intestinal enterococci, to monitor the microbiological quality of water. However, water environments are now recognized as a secondary...

journal_title：Applied and environmental microbiology

authors： Berthe T,Ratajczak M,Clermont O,Denamur E,Petit F

更新日期：2013-08-01 00:00:00

abstract：:Two types of mesophilic, methanogenic bacteria were isolated in pure culture from anaerobic freshwater and marine mud with 2-propanol as the hydrogen donor. The freshwater strain (SK) was a Methanospirillum species, the marine, salt-requiring strain (CV), which had irregular coccoid cells, resembled Methanogenium sp. ...

journal_title：Applied and environmental microbiology

authors： Widdel F

更新日期：1986-05-01 00:00:00

abstract：:Thermoanaerobacter sp. strain X514 has great potential in biotechnology due to its capacity to ferment a range of C(5) and C(6) sugars to ethanol and other metabolites under thermophilic conditions. This study investigated the central metabolism of strain X514 via (13)C-labeled tracer experiments using either glucose ...

journal_title：Applied and environmental microbiology

authors： Feng X,Mouttaki H,Lin L,Huang R,Wu B,Hemme CL,He Z,Zhang B,Hicks LM,Xu J,Zhou J,Tang YJ

更新日期：2009-08-01 00:00:00