Detection of botulinum neurotoxin serotype A, B, and F proteolytic activity in complex matrices with picomolar to femtomolar sensitivity.

abstract：:A simple and efficient process for the production of a ruminant feed supplement, rich in crude protein (defined as total N X 6.25), by bacterial fermentation of cheese whey has been developed. The lactose in unpasteurized whey is fermented to lactate acid by Lactobacillus bulgaricus at a temperature of 43 degrees C an...

journal_title：Applied and environmental microbiology

authors： Reddy CA,Henderson HE,Erdman MD

更新日期：1976-12-01 00:00:00

abstract：:The survival of Azospirillum brasilense Cd and Sp-245 in the rhizosphere of wheat and tomato plants and in 23 types of plant-free sterilized soils obtained from a wide range of environments in Israel and Mexico was evaluated. Large numbers of A. brasilense cells were detected in all the rhizospheres tested, regardless...

journal_title：Applied and environmental microbiology

authors： Bashan Y,Puente ME,Rodriguez-Mendoza MN,Toledo G,Holguin G,Ferrera-Cerrato R,Pedrin S

更新日期：1995-05-01 00:00:00

abstract：:Volume 61, no. 12, p. 4505, Introduction, column 1, line 13, and column 2, line 5: reference 19, which refers to a toxicity test based on the inhibition of (beta)-glucuronidase, not (beta)-galactosidase, in E. coli, should not be cited. [This corrects the article on p. 4505 in vol. 61.]. ...

journal_title：Applied and environmental microbiology

authors： Van Poucke SO,Nelis HJ

更新日期：1996-02-01 00:00:00

abstract：:Four different green fluorescent protein (GFP)-based whole-cell biosensors were created based on the DNA damage inducible SOS response of Escherichia coli in order to evaluate the sensitivity of individual SOS promoters toward genotoxic substances. Treatment with the known carcinogen N-methyl-N'-nitro-N-nitrosoguanidi...

journal_title：Applied and environmental microbiology

authors： Norman A,Hestbjerg Hansen L,Sørensen SJ

更新日期：2005-05-01 00:00:00

abstract：:A mechanistic understanding of how new phenotypes develop to overcome the loss of a gene product provides valuable insight on both the metabolic and regulatory functions of the lost gene. The pgi gene, whose product catalyzes the second step in glycolysis, was deleted in a growth-optimized Escherichia coli K-12 MG1655...

journal_title：Applied and environmental microbiology

authors： McCloskey D,Xu S,Sandberg TE,Brunk E,Hefner Y,Szubin R,Feist AM,Palsson BO

更新日期：2018-09-17 00:00:00

abstract：:To determine whether the pathogenic variant of Lyme disease spirochetes, isolate A14S, is perpetuated in a particular reservoir-vector relationship, we screened vector ticks in various Central European sites for a related spirochete and determined its host association. A14S-like spirochetes infect numerous questing ti...

journal_title：Applied and environmental microbiology

authors： Richter D,Schlee DB,Allgöwer R,Matuschka FR

更新日期：2004-11-01 00:00:00

abstract：:The influence of modified-atmosphere packaging, storage temperature, and time on survival and growth of Escherichia coli O157:H7 inoculated onto shredded lettuce, sliced cucumber, and shredded carrot was determined. Growth of psychotrophic and mesophilic microorganisms and changes in pH and sensory qualities of vegeta...

journal_title：Applied and environmental microbiology

authors： Abdul-Raouf UM,Beuchat LR,Ammar MS

更新日期：1993-07-01 00:00:00

abstract：:A spontaneous rpsL mutant of Thermus thermophilus was isolated in a search for new selection markers for this organism. This new allele, named rpsL1, encodes a K47R/K57E double mutant S12 ribosomal protein that confers a streptomycin-dependent (SD) phenotype to T. thermophilus. Models built on the available three-dime...

journal_title：Applied and environmental microbiology

authors： Blas-Galindo E,Cava F,López-Viñas E,Mendieta J,Berenguer J

更新日期：2007-08-01 00:00:00

abstract：:Currently only five (SEA-SEE) out of 27 known staphylococcal enterotoxins can be analyzed using commercially available kits.Six genes (seg, sei, sem, sen, seo, and seu), encoding putative and undetectable enterotoxins, are located on the enterotoxin gene cluster (egc) which is part of the Staphylococcus aureus genomic...

journal_title：Applied and environmental microbiology

authors： Schwendimann L,Merda D,Berger T,Denayer S,Feraudet Tarisse C,Kläui AJ,Messio S,Mistou MY,Nia Y,Hennekinne JA,Graber HU

更新日期：2020-12-18 00:00:00

abstract：:The potential of bacteriophage as an alternative biocontrol agent has recently been revisited due to the widespread occurrence of antibiotic-resistant bacteria. We isolated a virulent bacteriophage, SPC35, that can infect both Salmonella enterica serovar Typhimurium and Escherichia coli. Morphological analysis by tran...

journal_title：Applied and environmental microbiology

authors： Kim M,Ryu S

更新日期：2011-03-01 00:00:00

abstract：:Infectious hematopoietic necrosis virus (IHNV) is a pathogen of young salmon and trout. Viral epizootics among these fish in private and public rearing facilities have been a problem in the northwestern United States from California to Alaska, and an IHNV vaccine has been sought by the aquaculture experts. Since an IH...

journal_title：Applied and environmental microbiology

authors： Engelking HM,Harry JB,Leong JA

更新日期：1991-05-01 00:00:00

abstract：:Twenty-four Escherichia coli strains producing standard colicins were evaluated for inhibitory activity against 27 diarrheagenic E. coli strains of serotypes O15:H-, O26:(H11, H-), and O111:(H8, H11, H-), including O157:H7, representing diarrheagenic E. coli clones, 3, 4, 8, 9, and 10. Overlay techniques were used to ...

journal_title：Applied and environmental microbiology

authors： Murinda SE,Roberts RF,Wilson RA

更新日期：1996-09-01 00:00:00

abstract：:Resistance to hops is a prerequisite for the capability of lactic acid bacteria to grow in beer and thus cause beer spoilage. Bactericidal hop compounds, mainly iso-alpha-acids, are described as ionophores which exchange H+ for cellular divalent cations, e.g., Mn2+, and thus dissipate ion gradients across the cytoplas...

journal_title：Applied and environmental microbiology

authors： Behr J,Israel L,Gänzle MG,Vogel RF

更新日期：2007-05-01 00:00:00

abstract：:Pyruvate carboxylase (PYC) is an ecologically, medically, and industrially important enzyme. It is widespread in all three domains of life, the archaea, bacteria, and eukarya. PYC catalyzes ATP-dependent carboxylation of pyruvate to oxaloacetate. Detailed structure-function studies of this enzyme have been hampered du...

journal_title：Applied and environmental microbiology

authors： Lai H,Kraszewski JL,Purwantini E,Mukhopadhyay B

更新日期：2006-12-01 00:00:00

abstract：:Batch cultures of a lithotrophic Fe(II)-oxidizing bacterium, strain BrT, isolated from the rhizosphere of a wetland plant, were grown in bioreactors and used to determine the significance of microbial Fe(II) oxidation at circumneutral pH and to identify abiotic variables that affect the partitioning between microbial ...

journal_title：Applied and environmental microbiology

authors： Neubauer SC,Emerson D,Megonigal JP

更新日期：2002-08-01 00:00:00

abstract：:The initiation of methanogenesis in refuse occurs under high volatile fatty acid (VFA) concentration and low pH (5.5 to 6.25), which generally are reported to inhibit methanogenic Archaea. One hypothesized mechanism for the initiation of methanogenesis in refuse decomposition is the presence of pH-neutral niches withi...

journal_title：Applied and environmental microbiology

authors： Staley BF,de Los Reyes FL 3rd,Barlaz MA

更新日期：2011-04-01 00:00:00

abstract：:The fatty acid methyl ester composition of a total of 71 marine strains representing the genera Alteromonas, Deleya, Oceanospirillum, and Vibrio was determined by gas-liquid chromatographic analysis. Over 70 different fatty acids were found. The predominant fatty acids were 16:0, 16:1 cis 9, summed-in-feature (SIF) 4 ...

journal_title：Applied and environmental microbiology

authors： Bertone S,Giacomini M,Ruggiero C,Piccarolo C,Calegari L

更新日期：1996-06-01 00:00:00

abstract：:An extracellular protein with strong absorption at 406 nm was purified from cell-free culture fluid of latex-grown Xanthomonas sp. strain 35Y. This protein was identical to the gene product of a recently characterized gene cloned from Xanthomonas sp., as revealed by determination of m/z values and sequencing of select...

journal_title：Applied and environmental microbiology

authors： Braaz R,Fischer P,Jendrossek D

更新日期：2004-12-01 00:00:00

abstract：:A technique to determine which nutrients limit bacterial growth in soil was developed. The method was based on measuring the thymidine incorporation rate of bacteria after the addition of C, N, and P in different combinations to soil samples. First, the thymidine incorporation method was tested in two different soils:...

journal_title：Applied and environmental microbiology

authors： Aldén L,Demoling F,Bååth E

更新日期：2001-04-01 00:00:00

abstract：:A heat-stable extracellular protease from Pseudomonas fluorescens was purified by chromatography on a DEAE-cellulose column and gel filtration on a Sephadex G100 column. The homogeneous enzyme preparation was used to prepare antiserum in rabbits. The rabbit antiserum was used to study the antigenic relatedness of prot...

journal_title：Applied and environmental microbiology

authors： Jackman DM,Bartlett FM,Patel TR

更新日期：1983-07-01 00:00:00

abstract：:In the United States, the 2009 pandemic influenza A (H1N1) virus (pH1N1) infected almost 20% of the population and caused >200,000 hospitalizations and >10,000 deaths from April 2009 to April 2010. On 24 April 2009, the CDC posted interim guidance on infection control measures in health care settings explicitly for pH...

journal_title：Applied and environmental microbiology

authors： Coulliette AD,Perry KA,Edwards JR,Noble-Wang JA

更新日期：2013-04-01 00:00:00

abstract：:The metabolism of aflatoxin B1 to aflatoxins M1 and Q1 by rat liver microsomes from animals pretreated with polychlorinated or polybrominated biphenyl congeners depended on the structure of the halogenated biphenyl inducers. Microsomes from rats treated with phenobarbital (PB) or halogenated biphenyls that exhibit PB-...

journal_title：Applied and environmental microbiology

authors： Halvorson MR,Phillips TD,Safe SH,Robertson LW

更新日期：1985-04-01 00:00:00

abstract：:An extracellular serine protease produced by a mutant, M1, derived from Streptomyces albogriseolus S-3253 that no longer produces a protease inhibitor (Streptomyces subtilisin inhibitor [SSI]) was isolated. A 20-kDa protein was purified by its affinity for SSI and designated SAM-P20. The amino acid sequence of the ami...

journal_title：Applied and environmental microbiology

authors： Taguchi S,Odaka A,Watanabe Y,Momose H

更新日期：1995-01-01 00:00:00

abstract：:Acclimation of a sandy soil to an air-natural gas mixture stimulated the biological oxidation of chloroform to carbon dioxide. Acetylene and methane inhibited chloroform oxidation. Chloroform oxidation continued up to 31 days in the absence of methane. Chloroform oxidation rates increased at chloroform concentrations ...

journal_title：Applied and environmental microbiology

authors： Strand SE,Shippert L

更新日期：1986-07-01 00:00:00

abstract：:[This corrects the article on p. 118 in vol. 43.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1982-04-01 00:00:00

abstract：:Very little is known about the microbial composition of animal bedding wastes, including poultry litter, and what is known has been deduced from standard culture methods, by which some fastidious organisms that exist in the environment may not be detected. We evaluated the bacterial composition of poultry litter by us...

journal_title：Applied and environmental microbiology

authors： Lu J,Sanchez S,Hofacre C,Maurer JJ,Harmon BG,Lee MD

更新日期：2003-02-01 00:00:00

abstract：:By using an autoradiographic technique, one can follow, during ripening of a cheese, the distribution, size, and metabolic activity of microcolonies. Fragments of cheese were labeled with [H]leucine, fixed, and mounted in epoxy resin. After exposure and development, sections were examined by optical microscopy. In Cam...

journal_title：Applied and environmental microbiology

authors： Reyrolle J,Letellier F

更新日期：1979-12-01 00:00:00

abstract：:From necrotic tissue of a Nashi pear tree, 24 Erwinia pyrifoliae strains, found to be identical by pulsed-field gel electrophoresis analysis, were isolated. Thirteen strains were not virulent on immature pears and did not induce a hypersensitive response in tobacco leaves. The defective gene hrpL was complemented with...

journal_title：Applied and environmental microbiology

authors： Jock S,Kim WS,Barny MA,Geider K

更新日期：2003-01-01 00:00:00

abstract：:Hydantoin racemase from Sinorhizobium meliloti was functionally expressed in Escherichia coli. The native form of the enzyme was a homotetramer with a molecular mass of 100 kDa. The optimum temperature and pH for the enzyme were 40 degrees C and 8.5, respectively. The enzyme showed a slight preference for hydantoins w...

journal_title：Applied and environmental microbiology

authors： Martínez-Rodríguez S,Las Heras-Vázquez FJ,Mingorance-Cazorla L,Clemente-Jiménez JM,Rodríguez-Vico F

更新日期：2004-01-01 00:00:00

abstract：:Seabird ticks are known reservoirs of bacterial pathogens of medical importance; however, ticks parasitizing tropical seabirds have received less attention than their counterparts from temperate and subpolar regions. Recently, Rickettsia africae was described to infect seabird ticks of the western Indian Ocean and New...

journal_title：Applied and environmental microbiology

authors： Wilkinson DA,Dietrich M,Lebarbenchon C,Jaeger A,Le Rouzic C,Bastien M,Lagadec E,McCoy KD,Pascalis H,Le Corre M,Dellagi K,Tortosa P

更新日期：2014-06-01 00:00:00