Abstract:
:The fungus Fusarium oxysporum produced a D-amino acid oxidase (EC 1. 4.3.3) in a medium containing glucose as the carbon and energy source and ammonium sulfate as the nitrogen source. The specific D-amino acid oxidase activity was increased up to 12.5-fold with various D-amino acids or their corresponding derivatives as inducers. The best inducers were D-alanine (2.7 microkat/g of dry biomass) and D-3-aminobutyric acid (2.6 microkat/g of dry biomass). The addition of zinc ions was necessary to permit the induction of peroxisomal D-amino acid oxidase. Bioreactor cultivations were performed on a 50-liter scale, yielding a volumetric D-amino acid oxidase activity of 17 microkat liter(-1) with D-alanine as an inducer. Under oxygen limitation, the volumetric activity was increased threefold to 54 microkat liter(-1) (3,240 U liter(-1)).
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Gabler M,Fischer Ldoi
10.1128/AEM.65.8.3750-3753.1999keywords:
subject
Has Abstractpub_date
1999-08-01 00:00:00pages
3750-3issue
8eissn
0099-2240issn
1098-5336journal_volume
65pub_type
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