Novel dehalogenase mechanism for 2,3-dichloro-1-propanol utilization in Pseudomonas putida strain MC4.

abstract：:We describe here an improved method for isolating, purifying, and cloning DNA from diverse soil microbiota. Soil microorganisms were extracted from soils and embedded and lysed within an agarose plug. Nucleases that copurified with the metagenomic DNA were removed by incubating plugs with a high-salt and -formamide so...

journal_title：Applied and environmental microbiology

authors： Liles MR,Williamson LL,Rodbumrer J,Torsvik V,Goodman RM,Handelsman J

更新日期：2008-05-01 00:00:00

abstract：:Swarming Proteus spp. were detected with the use of proteometry (a most-probable-number technique) in the fecal material of selected animal species and in raw sewage from a local sewage treatment plant. Proteus spp. were not detected in any of several soil and freshwater samples examined. Since rat feces harbored high...

journal_title：Applied and environmental microbiology

authors： Stahl ML,Williams FD

更新日期：1981-03-01 00:00:00

abstract：:Several microorganisms were examined for their abilities to convert S-nicotine into nornicotine. Five microorganisms including Microsporum gypseum (ATCC 11395) produced nornicotine and three unknown metabolites. M. gypseum efficiently reduced nicotine-1'-N-oxide to nicotine, but no nornicotine was obtained when the N-...

journal_title：Applied and environmental microbiology

authors： Sindelar RD,Rosazza JP,Barfknecht CF

更新日期：1979-11-01 00:00:00

abstract：:The hydroaromatic acids shikimate and quinate, which may be available as carbon sources in the soil, supported production of only low levels of acidic exopolysaccharide by Bradyrhizobium japonicum. Exopolysaccharide production (micrograms per 10 cells) was 4.9 on quinate and 4.5 on shikimate; in comparison, it was 128...

journal_title：Applied and environmental microbiology

authors： Tully RE

更新日期：1988-06-01 00:00:00

abstract：:Most denitrifiers produce nitrous oxide (N(2)O) instead of dinitrogen (N(2)) under aerobic conditions. We isolated and characterized novel aerobic denitrifiers that produce low levels of N(2)O under aerobic conditions. We monitored the denitrification activities of two of the isolates, strains TR2 and K50, in batch an...

journal_title：Applied and environmental microbiology

authors： Takaya N,Catalan-Sakairi MA,Sakaguchi Y,Kato I,Zhou Z,Shoun H

更新日期：2003-06-01 00:00:00

abstract：:The chlorine resistance of cysts of the flagellate protozoan Giardia muris was examined. This organism, which is pathogenic to mice, is being considered as a model for the inactivation of the human pathogen Giardia lamblia. Excystation was used as the criterion for cyst viability. Experiments were performed at pH 5, 7...

journal_title：Applied and environmental microbiology

authors： Leahy JG,Rubin AJ,Sproul OJ

更新日期：1987-07-01 00:00:00

abstract：:The abundant growth of molds and thermophilic actinomycetes in stored hay decreases its quality and can be hazardous for the producer who inhales these contaminants when the moldy hay is fed in closed barns. These microbes are responsible for a respiratory disease called farmer's lung. Products, including bacterial cu...

journal_title：Applied and environmental microbiology

authors： Duchaine C,Lavoie MC,Cormier Y

更新日期：1995-12-01 00:00:00

abstract：:Indirect exposure to waterborne viruses increases the risk of infection, especially among children with frequent hand-to-mouth contacts. Here, we quantified the transfer of one bacteriophage (MS2) and two enteric viruses (adenovirus and coxsackievirus) from liquid to skin. MS2, a commonly used enteric virus surrogate,...

journal_title：Applied and environmental microbiology

authors： Pitol AK,Bischel HN,Boehm AB,Kohn T,Julian TR

更新日期：2018-10-30 00:00:00

abstract：:We examined growth of mixed microbial cultures (13 fungal species and one actinomycete species) and production of volatile compounds (VOCs) in typical building materials in outside walls, separating walls, and bathroom floors at various relative humidities (RHs) of air. Air samples from incubation chambers were adsorb...

journal_title：Applied and environmental microbiology

authors： Korpi A,Pasanen AL,Pasanen P

更新日期：1998-08-01 00:00:00

abstract：:Two thermostable lipases were isolated and characterized from Thermosyntropha lipolytica DSM 11003, an anaerobic, thermophilic, alkali-tolerant bacterium which grows syntrophically with methanogens on lipids such as olive oil, utilizing only the liberated fatty acid moieties but not the glycerol. Lipases LipA and LipB...

journal_title：Applied and environmental microbiology

authors： Salameh MA,Wiegel J

更新日期：2007-12-01 00:00:00

abstract：:Microbiological, genomic and transcriptomic analyses were used to examine three species from the bacterial genus Caldicellulosiruptor with respect to their capacity to convert the carbohydrate content of lignocellulosic biomass at 70°C to simple sugars, acetate, lactate, CO2, and H2. Caldicellulosiruptor bescii, C. kr...

journal_title：Applied and environmental microbiology

authors： Zurawski JV,Conway JM,Lee LL,Simpson HJ,Izquierdo JA,Blumer-Schuette S,Nookaew I,Adams MW,Kelly RM

更新日期：2015-10-01 00:00:00

abstract：:Cytochrome c(3) from Desulfovibrio vulgaris Miyazaki F was successfully expressed in the facultative aerobe Shewanella oneidensis MR-1 under anaerobic, microaerophilic, and aerobic conditions, with yields of 0.3 to 0.5 mg of cytochrome/g of cells. A derivative of the broad-host-range plasmid pRK415 containing the cyto...

journal_title：Applied and environmental microbiology

authors： Ozawa K,Tsapin AI,Nealson KH,Cusanovich MA,Akutsu H

更新日期：2000-09-01 00:00:00

abstract：:Protein S is a major spore coat protein of Myxococcus xanthus, consisting of two homologous domains, the N-terminal domain (NTD) and the C-terminal domain, both of which contain a Ca(2+)-binding site. Protein S tightly binds to myxospores in a Ca(2+)-dependent manner. Here, we constructed a novel expression vector, pC...

journal_title：Applied and environmental microbiology

authors： Kobayashi H,Yoshida T,Inouye M

更新日期：2009-08-01 00:00:00

abstract：:A newly isolated rod-shaped, gram-negative anaerobic bacterium from human feces, named Julong 732, was found to be capable of metabolizing the isoflavone dihydrodaidzein to S-equol under anaerobic conditions. The metabolite, equol, was identified by using electron impact ionization mass spectrometry, (1)H and (13)C nu...

journal_title：Applied and environmental microbiology

authors： Wang XL,Hur HG,Lee JH,Kim KT,Kim SI

更新日期：2005-01-01 00:00:00

abstract：:Bacteria able to perform dissimilatory nitrate reduction to ammonium were isolated from low-oxygen masses in the Baltic Sea. In liquid media enriched with NO(3) and incubated anaerobically, the NH(4)-producing isolates transformed 25 to 72% of the NO(3) to NH(4). ...

journal_title：Applied and environmental microbiology

authors： Samuelsson MO,Rönner U

更新日期：1982-11-01 00:00:00

abstract：:The structural gene coding for the lysine-sensitive aspartokinase II of the methylotrophic thermotolerant Bacillus sp. strain MGA3 was cloned from a genomic library by complementation of an Escherichia coli auxotrophic mutant lacking all three aspartokinase isozymes. The nucleotide sequence of the entire 2.2-kb PstI f...

journal_title：Applied and environmental microbiology

authors： Schendel FJ,Flickinger MC

更新日期：1992-09-01 00:00:00

abstract：:An extracellular serine protease produced by a mutant, M1, derived from Streptomyces albogriseolus S-3253 that no longer produces a protease inhibitor (Streptomyces subtilisin inhibitor [SSI]) was isolated. A 20-kDa protein was purified by its affinity for SSI and designated SAM-P20. The amino acid sequence of the ami...

journal_title：Applied and environmental microbiology

authors： Taguchi S,Odaka A,Watanabe Y,Momose H

更新日期：1995-01-01 00:00:00

abstract：:A microscopically pure enrichment culture of a gram-negative anaerobic bacterium, in the present article referred to as PER-K23, was isolated from an anaerobic packed-bed column in which tetrachloroethene (PCE) was reductively transformed to ethane via trichloroethene (TCE), cis-1,2-dichloroethene (cis-1,2-DCE), chlor...

journal_title：Applied and environmental microbiology

authors： Holliger C,Schraa G,Stams AJ,Zehnder AJ

更新日期：1993-09-01 00:00:00

abstract：:In two previous studies dealing with lactic acid bacteria (LAB) from modified-atmosphere-packaged (MAP) broiler products and a broiler processing plant, several isolates remained unidentified. According to 16S rRNA gene sequence analysis, 36 isolates were assigned to the genus Enterococcus. Numerical analysis of combi...

journal_title：Applied and environmental microbiology

authors： Rahkila R,Johansson P,Säde E,Björkroth J

更新日期：2011-02-01 00:00:00

abstract：:A rapid and simple approach to the small-subunit (SSU) rRNA-based quantitative detection of a specific group of microorganisms in complex ecosystems has been developed. The method employs sequence-specific cleavage of rRNA molecules with oligonucleotides and RNase H. Defined mixtures of SSU rRNAs were mixed with an ol...

journal_title：Applied and environmental microbiology

authors： Uyeno Y,Sekiguchi Y,Sunaga A,Yoshida H,Kamagata Y

更新日期：2004-06-01 00:00:00

abstract：:Pseudomonas aeruginosa is an opportunistic human pathogen and has been established as a model organism to study bacterial biofilm formation. At least three exopolysaccharides (alginate, Psl, and Pel) contribute to the formation of biofilms in this organism. Here mutants deficient in the production of one or more of th...

journal_title：Applied and environmental microbiology

authors： Ghafoor A,Hay ID,Rehm BH

更新日期：2011-08-01 00:00:00

abstract：:Nisin is a small gene-encoded antimicrobial protein produced by Lactococcus lactis that contains unusual dehydroalanine and dehydrobutyrine residues. The reactivity of these residues toward nucleophiles was explored by reacting nisin with a variety of mercaptans. The kinetics of reaction with 2-mercaptoethane-sulfonat...

journal_title：Applied and environmental microbiology

authors： Liu W,Hansen JN

更新日期：1990-08-01 00:00:00

abstract：:Fluorescent antibodies (FAs) prepared against 16 ammonia-oxidizing nitrifying bacteria were examined as to cross-reactivity in heterologous FA staining tests. Virtually all cross-reactions to the seven Nitrosomonas FAs were confined to the Nitrosomonas isolates. The five Nitrosospira, three Nitrosolobus, and one Nitro...

journal_title：Applied and environmental microbiology

authors： Belser LW,Schmidt EL

更新日期：1978-10-01 00:00:00

abstract：:Genomic probes were used to investigate hepatitis A virus (HAV) and enterovirus RNAs in two types of shellfish from natural beds (Atlantic coast, France). After elution concentration, nucleic acid extracted by proteinase K and purified by phenol-chloroform and ethanol precipitation was assayed by dot blot hybridizatio...

journal_title：Applied and environmental microbiology

authors： Le Guyader F,Apaire-Marchais V,Brillet J,Billaudel S

更新日期：1993-11-01 00:00:00

abstract：:Two anaerobic bacteria were isolated from polyethylene glycol (PEG)-degrading, methanogenic, enrichment cultures obtained from a municipal sludge digester. One isolate, identified as Desulfovibrio desulfuricans (strain DG2), metabolized oligomers ranging from ethylene glycol (EG) to tetraethylene glycol. The other iso...

journal_title：Applied and environmental microbiology

authors： Dwyer DF,Tiedje JM

更新日期：1986-10-01 00:00:00

abstract：:Glutaminase is an enzyme that catalyzes the hydrolysis of l-glutamine to l-glutamate, and it plays an important role in the production of fermented foods by enhancing the umami taste. By using the genome sequence and expressed sequence tag data available for Aspergillus oryzae RIB40, we cloned a novel glutaminase gene...

journal_title：Applied and environmental microbiology

authors： Ito K,Matsushima K,Koyama Y

更新日期：2012-08-01 00:00:00

abstract：:Pseudomonas aeruginosa JB2 was isolated from a polychlorinated biphenyl-contaminated soil by enrichment culture containing 2-chlorobenzoate as the sole carbon source. Strain JB2 was subsequently found also to grow on 3-chlorobenzoate, 2,3- and 2,5-dichlorobenzoates, 2,3,5-trichlorobenzoate, and a wide range of other m...

journal_title：Applied and environmental microbiology

authors： Hickey WJ,Focht DD

更新日期：1990-12-01 00:00:00

abstract：:Free fatty acids are important flavor compounds in cheese. Propionibacterium freudenreichii is the main agent of their release through lipolysis in Swiss cheese. Our aim was to identify the esterase(s) involved in lipolysis by P. freudenreichii. We targeted two previously identified esterases: one secreted esterase, P...

journal_title：Applied and environmental microbiology

authors： Abeijón Mukdsi MC,Falentin H,Maillard MB,Chuat V,Medina RB,Parayre S,Thierry A

更新日期：2014-01-01 00:00:00

abstract：:Free-living soybean rhizobia and Bradyrhizobium spp. (lupine) have the ability to catabolize ethanol. Of the 30 strains of rhizobia examined, only the fast- and slow-growing soybean rhizobia and the slow-growing Bradyrhizobium sp. (lupine) were capable of using ethanol as a sole source of carbon and energy for growth....

journal_title：Applied and environmental microbiology

authors： Sadowsky MJ,Bohlool BB

更新日期：1986-10-01 00:00:00

abstract：:DNA was extracted from dry standing dead Spartina alterniflora stalks as well as dry Spartina wrack from the North Inlet (South Carolina) and Sapelo Island (Georgia) salt marshes. Partial nifH sequences were PCR amplified, the products were separated by denaturing gradient gel electrophoresis (DGGE), and the prominent...

journal_title：Applied and environmental microbiology

authors： Lovell CR,Friez MJ,Longshore JW,Bagwell CE

更新日期：2001-11-01 00:00:00