Sequence-specific cleavage of small-subunit (SSU) rRNA with oligonucleotides and RNase H: a rapid and simple approach to SSU rRNA-based quantitative detection of microorganisms.

abstract：:This study describes the genotypic characteristics of a collection of 100 multidrug-resistant (MDR) Escherichia coli strains recovered from cattle and the farm environment in Ireland in 2007. The most prevalent antimicrobial resistance identified was to streptomycin (100%), followed by tetracycline (99%), sulfonamides...

journal_title：Applied and environmental microbiology

authors： Karczmarczyk M,Walsh C,Slowey R,Leonard N,Fanning S

更新日期：2011-10-01 00:00:00

abstract：:Sputter coating of leaf litter microbe samples provides scanning electron microscope images with greater information content than either vacuum evaporation of thin metal coatings or tissue conductance. ...

journal_title：Applied and environmental microbiology

authors： Draggan S

更新日期：1976-02-01 00:00:00

abstract：UNLABELLED:Mechanistic studies of many processes in Agrobacterium tumefaciens have been hampered by a lack of genetic tools for characterization of essential genes. In this study, we used a Tn7-based method for inducible control of transcription from an engineered site on the chromosome. We demonstrate that this method...

journal_title：Applied and environmental microbiology

authors： Figueroa-Cuilan W,Daniel JJ,Howell M,Sulaiman A,Brown PJ

更新日期：2016-07-29 00:00:00

abstract：:Laying hens were individually caged at 20 weeks of age and tested for fecal excretion of Campylobacter jejuni (minimum level of detection was 100 cfu/g) during a 42-week period. Peak rates of C. jejuni isolation (approximately 25% of hens positive) occurred at two different times, in October and in late April to early...

journal_title：Applied and environmental microbiology

authors： Doyle MP

更新日期：1984-03-01 00:00:00

abstract：:Biotin-labeled DNA probes prepared from whole plasmids (5.5 and 4.8 kilobases) of two lactobacillus strains (Lactobacillus delbrueckii 21 and Lactobacillus reuteri 100-23) were used to detect the homologous bacteria in microtome-cryostat-prepared sections of murine forestomach. The forestomach sections were incubated ...

journal_title：Applied and environmental microbiology

authors： Tannock GW

更新日期：1989-02-01 00:00:00

abstract：:The Atlantis Massif rises 4,000 m above the seafloor near the Mid-Atlantic Ridge and consists of rocks uplifted from Earth's lower crust and upper mantle. Exposure of the mantle rocks to seawater leads to their alteration into serpentinites. These aqueous geochemical reactions, collectively known as the process of ser...

journal_title：Applied and environmental microbiology

authors： Motamedi S,Orcutt BN,Früh-Green GL,Twing KI,Pendleton HL,Brazelton WJ

更新日期：2020-05-19 00:00:00

abstract：:A newly isolated rod-shaped, gram-negative anaerobic bacterium from human feces, named Julong 732, was found to be capable of metabolizing the isoflavone dihydrodaidzein to S-equol under anaerobic conditions. The metabolite, equol, was identified by using electron impact ionization mass spectrometry, (1)H and (13)C nu...

journal_title：Applied and environmental microbiology

authors： Wang XL,Hur HG,Lee JH,Kim KT,Kim SI

更新日期：2005-01-01 00:00:00

abstract：:Aerobic activated sludge granules are dense, spherical biofilms which can strongly improve purification efficiency and sludge settling in wastewater treatment processes. In this study, the structure and development of different granule types were analyzed. Biofilm samples originated from lab-scale sequencing batch rea...

journal_title：Applied and environmental microbiology

authors： Weber SD,Ludwig W,Schleifer KH,Fried J

更新日期：2007-10-01 00:00:00

abstract：:Florfenicol resistance was analyzed in 230 enteric pig isolates collected between 1998 and 2006. PCR, plasmid profiling, Southern blot hybridization, and a mixed-broth conjugation assay suggested the intra- and interspecies plasmid-mediated transfer of florfenicol resistance among the isolates that exhibited MICs for ...

journal_title：Applied and environmental microbiology

authors： Rayamajhi N,Cha SB,Kang ML,Lee SI,Lee HS,Yoo HS

更新日期：2009-09-01 00:00:00

abstract：:Organophosphate pesticides used in agriculture can pose health risks to humans and wildlife. We hypothesized that dietary supplementation with Lactobacillus, a genus of commensal bacteria, would reduce absorption and toxicity of consumed organophosphate pesticides (parathion and chlorpyrifos [CP]). Several Lactobacill...

journal_title：Applied and environmental microbiology

authors： Trinder M,McDowell TW,Daisley BA,Ali SN,Leong HS,Sumarah MW,Reid G

更新日期：2016-09-30 00:00:00

abstract：:The filamentous, nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120 accumulates sucrose as a compatible solute against salt stress. Sucrose-phosphate synthase activity, which is responsible for the sucrose synthesis, is increased by salt stress, but the mechanism underlying the regulation of sucrose synthesis...

journal_title：Applied and environmental microbiology

authors： Ehira S,Kimura S,Miyazaki S,Ohmori M

更新日期：2014-09-01 00:00:00

abstract：:A polyphasic approach involving cultivation, direct viable counts, rRNA-based phylogenetic classification, and in situ probing was applied for the characterization of the dominant microbial population in a municipal drinking water distribution system. A total of 234 bacterial strains cultivated on R2A medium were scre...

journal_title：Applied and environmental microbiology

authors： Kalmbach S,Manz W,Szewzyk U

更新日期：1997-11-01 00:00:00

abstract：:A small spirillum, designated 5175, was isolated from an anaerobic enrichment culture for Desulfuromonas in which the major medium constituents were acetate and elemental sulfur. The organisms grew only under anaerobic or microaerophilic conditions. Elemental sulfur was formed anaerobically in a malate-sulfide medium,...

journal_title：Applied and environmental microbiology

authors： Wolfe RS,Penning N

更新日期：1977-02-01 00:00:00

abstract：:Sulfidogenic consortia enriched from an estuarine sediment were maintained on either 2-, 3-, or 4-chlorophenol as the only source of carbon and energy for over 5 years. The enrichment culture on 4-chlorophenol was the most active and this consortium was selected for further characterization. Utilization of chloropheno...

journal_title：Applied and environmental microbiology

authors： Häggblom MM,Young LY

更新日期：1995-04-01 00:00:00

abstract：:A strain that efficiently degraded methyl tert-butyl ether (MTBE) was obtained by initial selection on the recalcitrant compound tert-butyl alcohol (TBA). This strain, a gram-positive methylotrophic bacterium identified as Mycobacterium austroafricanum IFP 2012, was also able to degrade tert-amyl methyl ether and tert...

journal_title：Applied and environmental microbiology

authors： François A,Mathis H,Godefroy D,Piveteau P,Fayolle F,Monot F

更新日期：2002-06-01 00:00:00

abstract：:Genome analysis revealed the existence of a putative transcriptional regulatory system governing CO metabolism in Thermococcus onnurineus NA1, a carboxydotrophic hydrogenogenic archaeon. The regulatory system is composed of CorQ with a 4-vinyl reductase domain and CorR with a DNA-binding domain of the LysR-type transc...

journal_title：Applied and environmental microbiology

authors： Kim MS,Choi AR,Lee SH,Jung HC,Bae SS,Yang TJ,Jeon JH,Lim JK,Youn H,Kim TW,Lee HS,Kang SG

更新日期：2015-03-01 00:00:00

abstract：:Bifidobacterium longum strain JDM301, a widely used commercial strain in China, encodes at least two MazEF-like modules and one RelBE-like toxin-antitoxin (TA) system in its chromosome, designated MazE1F1Bif, MazE2F2Bif, and RelBEBif, respectively. Bacterial TA systems play an important role in several stress response...

journal_title：Applied and environmental microbiology

authors： Wei Y,Li Y,Yang F,Wu Q,Liu D,Li X,Hua H,Liu X,Wang Y,Zheng K,Tang R

更新日期：2017-04-17 00:00:00

abstract：:[This corrects the article on p. 2610 in vol. 57.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1992-02-01 00:00:00

abstract：:The diversity of rhizobia that form symbioses with roots of black locust (Robinia pseudoacacia L.), an economically important leguminous tree species, was examined by inoculating seedling root zones with samples of soil collected from the United States, Canada, and China. Bacteria were isolated from nodules, subcultur...

journal_title：Applied and environmental microbiology

authors： McCray Batzli J,Graves WR,van Berkum P

更新日期：1992-07-01 00:00:00

abstract：:The effects of antibiotic production on rhizosphere microbial communities of field-grown Phaseolus vulgaris were assessed by using ribosomal intergenic spacer analysis. Inoculum strains of Rhizobium etli CE3 differing only in trifolitoxin production were used. Trifolitoxin production dramatically reduced the diversity...

journal_title：Applied and environmental microbiology

authors： Robleto EA,Borneman J,Triplett EW

更新日期：1998-12-01 00:00:00

abstract：:The photosynthetic bacterium Rhodobacter capsulatus can grow with short- to long-chain fatty acids as the sole carbon source (R. G. Kranz, K. K. Gabbert, T. A. Locke, and M. T. Madigan, Appl. Environ. Microbiol. 63:3003-3009, 1997). Concomitant with growth on fatty acids is the production to high levels of the polyest...

journal_title：Applied and environmental microbiology

authors： Kranz RG,Gabbert KK,Madigan MT

更新日期：1997-08-01 00:00:00

abstract：:Botulinum neurotoxin (BoNT), produced by neurotoxigenic clostridia, is the most potent biological toxin known and the causative agent of the paralytic disease botulism. The nutritional, environmental, and genetic regulation of BoNT synthesis, activation, stability, and toxin complex (TC) formation is not well studied....

journal_title：Applied and environmental microbiology

authors： Fredrick CM,Lin G,Johnson EA

更新日期：2017-06-16 00:00:00

abstract：:The geochemical cycling of cobalt (Co) has often been considered to be controlled by the scavenging and oxidation of Co(II) on the surface of manganese [Mn(III,IV)] oxides or manganates. Because Mn(II) oxidation in the environment is often catalyzed by bacteria, we have investigated the ability of Mn(II)-oxidizing bac...

journal_title：Applied and environmental microbiology

authors： Lee Y,Tebo BM

更新日期：1994-08-01 00:00:00

abstract：:Rsan-ver, a strain of Pseudomonas aeruginosa isolated at this department, was used for the development of a continuous process for biosurfactant production. The active compounds were identified as rhamnolipids. A final medium for production was designed in continuous culture by means of medium shifts, since the format...

journal_title：Applied and environmental microbiology

authors： Guerra-Santos L,Käppeli O,Fiechter A

更新日期：1984-08-01 00:00:00

abstract：:Campylobacter jejuni is a foodborne pathogen that is recognized as the leading cause of human bacterial gastroenteritis. The widespread use of antibiotics in medicine and in animal husbandry has led to an increased incidence of antibiotic resistance in Campylobacter In addition to a role in multidrug resistance (MDR),...

journal_title：Applied and environmental microbiology

authors： Vieira A,Ramesh A,Seddon AM,Karlyshev AV

更新日期：2017-10-31 00:00:00

abstract：:A bioassay method was developed to measure the average intensity within a UV disinfection reactor. The survival of spores of Bacillus subtilis was determined as a function of UV dose to prepare a standard curve. Spores were added to unknown systems, and the survival rate was used to determine the average intensity. A ...

journal_title：Applied and environmental microbiology

authors： Qualls RG,Johnson JD

更新日期：1983-03-01 00:00:00

abstract：:For PCR detection of Coxiella burnetii in various clinical specimens we developed a sample preparation method in which silica binding of DNA was used. This method was found to be fast, easily performed with large numbers of samples, and equally sensitive for all of the specimens tested (livers, spleens, placentas, hea...

journal_title：Applied and environmental microbiology

authors： Lorenz H,Jäger C,Willems H,Baljer G

更新日期：1998-11-01 00:00:00

abstract：:A highly specific medium was developed for the enumeration of lactate-utilizing bacteria in the rumen of sheep. This medium, which contained 2.0% lactate, 2.0% Trypticase, 0.2% yeast extract, and volatile fatty acids, hemin, and trace elements in place of rumen fluid, enabled high counts (42 x 10 to 190 x 10/g of inge...

journal_title：Applied and environmental microbiology

authors： Mackie RI,Heath S

更新日期：1979-09-01 00:00:00

abstract：:This paper explores the relationship between the genetic diversity of rhizobia and the morphological diversity of their plant hosts. Rhizobium galegae strains were isolated from nodules of wild Galega orientalis and Galega officinalis in the Caucasus, the center of origin for G. orientalis. All 101 isolates were chara...

journal_title：Applied and environmental microbiology

authors： Andronov EE,Terefework Z,Roumiantseva ML,Dzyubenko NI,Onichtchouk OP,Kurchak ON,Dresler-Nurmi A,Young JP,Simarov BV,Lindström K

更新日期：2003-02-01 00:00:00

abstract：:Pulsed-field gel electrophoresis established the linkage between recalled chocolate milk and a multistate invasive listeriosis outbreak during a four-product recall period. Listeria monocytogenes isolates from four hospitalized patients and an environmental dairy sample displayed AscI restriction endonuclease digestio...

journal_title：Applied and environmental microbiology

authors： Proctor ME,Brosch R,Mellen JW,Garrett LA,Kaspar CW,Luchansky JB

更新日期：1995-08-01 00:00:00