Expression of a tetraheme protein, Desulfovibrio vulgaris Miyazaki F cytochrome c(3), in Shewanella oneidensis MR-1.

Abstract:

:Cytochrome c(3) from Desulfovibrio vulgaris Miyazaki F was successfully expressed in the facultative aerobe Shewanella oneidensis MR-1 under anaerobic, microaerophilic, and aerobic conditions, with yields of 0.3 to 0.5 mg of cytochrome/g of cells. A derivative of the broad-host-range plasmid pRK415 containing the cytochrome c(3) gene from D. vulgaris Miyazaki F was used for transformation of S. oneidensis MR-1, resulting in the production of protein product that was indistinguishable from that produced by D. vulgaris Miyazaki F, except for the presence of one extra alanine residue at the N terminus.

journal_name

Appl Environ Microbiol

authors

Ozawa K,Tsapin AI,Nealson KH,Cusanovich MA,Akutsu H

doi

10.1128/aem.66.9.4168-4171.2000

keywords:

subject

Has Abstract

pub_date

2000-09-01 00:00:00

pages

4168-71

issue

9

eissn

0099-2240

issn

1098-5336

journal_volume

66

pub_type

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