Abstract:
:Cytochrome c(3) from Desulfovibrio vulgaris Miyazaki F was successfully expressed in the facultative aerobe Shewanella oneidensis MR-1 under anaerobic, microaerophilic, and aerobic conditions, with yields of 0.3 to 0.5 mg of cytochrome/g of cells. A derivative of the broad-host-range plasmid pRK415 containing the cytochrome c(3) gene from D. vulgaris Miyazaki F was used for transformation of S. oneidensis MR-1, resulting in the production of protein product that was indistinguishable from that produced by D. vulgaris Miyazaki F, except for the presence of one extra alanine residue at the N terminus.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Ozawa K,Tsapin AI,Nealson KH,Cusanovich MA,Akutsu Hdoi
10.1128/aem.66.9.4168-4171.2000keywords:
subject
Has Abstractpub_date
2000-09-01 00:00:00pages
4168-71issue
9eissn
0099-2240issn
1098-5336journal_volume
66pub_type
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