Recovery of spores of Clostridium botulinum in yeast extract agar and pork infusion agar after heat treatment.

Abstract:

:Yeast extract agar, pork infusion agar, and modifications of these media were used to recover heated Clostridium botulinum spores. The D- and z-values were determined. Two type A strains and one type B strain of C. botulinum were studied. In all cases the D-values were largest when the spores were recovered in yeast extract agar, compared to the D-values for spores recovered in pork infusion agar. The z-values for strains 62A and A16037 were largest when the spores were recovered in pork infusion agar. The addition of sodium bicarbonate and sodium thioglycolate to pork infusion agar resulted in D-values for C. botulinum 62A spores similar to those for the same spores recovered in yeast extract agar. The results suggest that sodium bicarbonate and sodium thioglycolate should be added to recovery media for heated C. botulinum spores to obtain maximum plate counts.

journal_name

Appl Environ Microbiol

authors

Odlaug TE,Pflug IJ

doi

10.1128/AEM.34.4.377-381.1977

subject

Has Abstract

pub_date

1977-10-01 00:00:00

pages

377-81

issue

4

eissn

0099-2240

issn

1098-5336

journal_volume

34

pub_type

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