Purification and characterization of two thermostable acetyl xylan esterases from Thermoanaerobacterium sp. strain JW/SL-YS485.

abstract：:Peat bogs dominated by Sphagnum spp. have relatively high areal rates of dimethyl sulfide (DMS) emission to the atmosphere. DMS was produced in anoxic slurries of Sphagnum peat with a linear time course and with an average rate of 40.4 (range, 22.0 to 68.6) nmol per liter of slurry (middot) day(sup-1) observed in nine...

journal_title：Applied and environmental microbiology

authors： Kiene RP,Hines ME

更新日期：1995-07-01 00:00:00

abstract：:Microscopic techniques were used in conjunction with normal microbiological procedures to examine the development of the spoilage microflora on the skin of chicken carcasses held at 2 degrees C. Pigmented and nonpigmented psychrotrophic pseudomonads were the major spoilage bacteria isolated at all stages of storage ex...

journal_title：Applied and environmental microbiology

authors： Thomas CJ,McMeekin TA

更新日期：1981-02-01 00:00:00

abstract：:From necrotic tissue of a Nashi pear tree, 24 Erwinia pyrifoliae strains, found to be identical by pulsed-field gel electrophoresis analysis, were isolated. Thirteen strains were not virulent on immature pears and did not induce a hypersensitive response in tobacco leaves. The defective gene hrpL was complemented with...

journal_title：Applied and environmental microbiology

authors： Jock S,Kim WS,Barny MA,Geider K

更新日期：2003-01-01 00:00:00

abstract：:CRISPR-based systems are emerging as the premier method to manipulate many cellular processes. In this study, a simple and efficient CRISPR interference (CRISPRi) system for targeted gene repression in archaea was developed. The Methanosarcina acetivorans CRISPR-Cas9 system was repurposed by replacing Cas9 with the ca...

journal_title：Applied and environmental microbiology

authors： Dhamad AE,Lessner DJ

更新日期：2020-10-15 00:00:00

abstract：:Bacillus subtilis RB14, which showed antibiotic activities against several phytopathogens in vitro by producing the antibiotics iturin A and surfactin, was subjected to a pot test to investigate its ability to suppress damping-off of tomato seedlings caused by Rhizoctonia solani. To facilitate recovery from soil, B. s...

journal_title：Applied and environmental microbiology

authors： Asaka O,Shoda M

更新日期：1996-11-01 00:00:00

abstract：:The antifungal activity of cecropin A(2-8)-melittin(6-9) hybrid undecapeptides, previously reported as active against plant pathogenic bacteria, was studied. A set of 15 sequences was screened in vitro against Fusarium oxysporum, Penicillium expansum, Aspergillus niger, and Rhizopus stolonifer. Most compounds were hig...

journal_title：Applied and environmental microbiology

authors： Badosa E,Ferré R,Francés J,Bardají E,Feliu L,Planas M,Montesinos E

更新日期：2009-09-01 00:00:00

abstract：:A new primer set was designed to specifically amplify ca. 1,100 bp of aoxB genes encoding the As(III) oxidase catalytic subunit from taxonomically diverse aerobic As(III)-oxidizing bacteria. Comparative analysis of AoxB protein sequences showed variable conservation levels and highlighted the conservation of essential...

journal_title：Applied and environmental microbiology

authors： Quéméneur M,Heinrich-Salmeron A,Muller D,Lièvremont D,Jauzein M,Bertin PN,Garrido F,Joulian C

更新日期：2008-07-01 00:00:00

abstract：:Escherichia coli O157:H7 has caused serious outbreaks of food-borne illness via transmission in a variety of food vehicles, including unpasteurized apple juice, dried salami, and spinach. To understand how this pathogen responds to the multiple stresses of the food environment, we compared global transcription pattern...

journal_title：Applied and environmental microbiology

authors： Bergholz TM,Vanaja SK,Whittam TS

更新日期：2009-06-01 00:00:00

abstract：:Western Lake Erie (Laurentian Great Lakes) is prone to annual cyanobacterial harmful algal blooms (cHABs) dominated by Microcystis spp. that often yield microcystin toxin concentrations exceeding the federal EPA recreational contact advisory of 8 μg liter-1 In August 2014, microcystin levels were detected in finished ...

journal_title：Applied and environmental microbiology

authors： McKindles KM,Manes MA,DeMarco JR,McClure A,McKay RM,Davis TW,Bullerjahn GS

更新日期：2020-10-28 00:00:00

abstract：:Undefined mesophilic mixed (DL) starter cultures are used in the production of continental cheeses and contain unknown strain mixtures of Lactococcus lactis and leuconostocs. The choice of starter culture affects the taste, aroma, and quality of the final product. To gain insight into the diversity of Lactococcus lact...

journal_title：Applied and environmental microbiology

authors： Frantzen CA,Kleppen HP,Holo H

更新日期：2018-01-17 00:00:00

abstract：:Mycophenolic acid (MPA) is the active ingredient in the increasingly important immunosuppressive pharmaceuticals CellCept (Roche) and Myfortic (Novartis). Despite the long history of MPA, the molecular basis for its biosynthesis has remained enigmatic. Here we report the discovery of a polyketide synthase (PKS), MpaC,...

journal_title：Applied and environmental microbiology

authors： Regueira TB,Kildegaard KR,Hansen BG,Mortensen UH,Hertweck C,Nielsen J

更新日期：2011-05-01 00:00:00

abstract：:The photosynthetic bacterium Rhodobacter sphaeroides is capable of producing H2 via nitrogenase when grown photoheterotrophically in the absence of N2. By using 14C-labeled malate, it was found that greater than 95% of this substrate was catabolized completely to CO2 during H2 production. About 60% of this catabolism ...

journal_title：Applied and environmental microbiology

authors： Macler BA,Bassham JA

更新日期：1988-11-01 00:00:00

abstract：:A TAT peptide-delivered molecular beacon was developed and utilized to enumerate murine norovirus 1, a human norovirus (NoV) surrogate, in RAW 264.7 cells. This allowed the detection of a single infective virus within 6 h, a 12-fold improvement in time required for viral detection and quantification compared to that r...

journal_title：Applied and environmental microbiology

authors： Ganguli PS,Chen W,Yates MV

更新日期：2011-08-01 00:00:00

abstract：:An inulinase-producing Microbulbifer sp. strain, JAM-3301, was isolated from a deep-sea sediment. An inulin operon that contained three open reading frames was cloned and sequenced. Two of the three genes were expressed. One product was an endo-inulinase, and the other was a β-fructofuranosidase. Both enzymes worked t...

journal_title：Applied and environmental microbiology

authors： Kobayashi T,Uchimura K,Deguchi S,Horikoshi K

更新日期：2012-04-01 00:00:00

abstract：:The human oral cavity is a complex ecosystem, and the alterations in salivary microbial communities are associated with both oral and nonoral diseases. The Mediterranean diet (MD) is a healthy dietary pattern useful for both prevention and treatment of several diseases. To further explore the effects of the MD on huma...

journal_title：Applied and environmental microbiology

authors： Laiola M,De Filippis F,Vitaglione P,Ercolini D

更新日期：2020-06-02 00:00:00

abstract：:Three particulate methane monooxygenase PCR primer sets (A189-A682, A189-A650, and A189-mb661) were investigated for their ability to assess methanotroph diversity in soils from three sites, i.e., heath, oak, and sitka, each of which was capable of oxidizing atmospheric concentrations of methane. Each PCR primer set w...

journal_title：Applied and environmental microbiology

authors： Bourne DG,McDonald IR,Murrell JC

更新日期：2001-09-01 00:00:00

abstract：:The activities of two enzymes, a 168-kDa protein and a 40-kDa protein, OmtA, purified from the filamentous fungus Aspergillus parasiticus were reported to convert the aflatoxin pathway intermediate sterigmatocystin to O-methylsterigmatocystin in vitro. Our initial goal was to determine if OmtA is necessary and suffici...

journal_title：Applied and environmental microbiology

authors： Lee LW,Chiou CH,Linz JE

更新日期：2002-11-01 00:00:00

abstract：:Cr(VI) (chromate) is a widespread environmental contaminant. Bacterial chromate reductases can convert soluble and toxic chromate to the insoluble and less toxic Cr(III). Bioremediation can therefore be effective in removing chromate from the environment, especially if the bacterial propensity for such removal is enha...

journal_title：Applied and environmental microbiology

authors： Park CH,Keyhan M,Wielinga B,Fendorf S,Matin A

更新日期：2000-05-01 00:00:00

abstract：:A high growth rate in bacterial cultures is usually achieved by optimizing growth conditions, but metabolism of the bacterium limits the maximal growth rate attainable on the carbon source used. This limitation can be circumvented by engineering the metabolism of the bacterium. Acinetobacter baylyi has become a model ...

journal_title：Applied and environmental microbiology

authors： Kannisto M,Aho T,Karp M,Santala V

更新日期：2014-11-01 00:00:00

abstract：:Escherichia coli ATCC 12806 was exposed to increasing subinhibitory concentrations of three biocides widely used in food industry facilities: trisodium phosphate (TSP), sodium nitrite (SNI), and sodium hypochlorite (SHY). The cultures exhibited an acquired tolerance to biocides (especially to SNI and SHY) after exposu...

journal_title：Applied and environmental microbiology

authors： Capita R,Riesco-Peláez F,Alonso-Hernando A,Alonso-Calleja C

更新日期：2014-02-01 00:00:00

abstract：:Four new primers and one published primer were used to PCR amplify hypervariable regions within the protozoal 18S rRNA gene to determine which primer pair provided the best identification and statistical analysis. PCR amplicons of 394 to 498 bases were generated from three primer sets, sequenced using Roche 454 pyrose...

journal_title：Applied and environmental microbiology

authors： Ishaq SL,Wright AD

更新日期：2014-09-01 00:00:00

abstract：:Products observed during anaerobic cyanide transformation are consistent with a hydrolytic pathway (HCN + H2O <--> HCONH2 + H2O <--> HCOOH + NH3). Formate, the most frequently observed product, was generally converted to bicarbonate. Formamide was rapidly hydrolyzed to formate upon exposure to the anaerobic consortium...

journal_title：Applied and environmental microbiology

authors： Fallon RD

更新日期：1992-09-01 00:00:00

abstract：:Freshwater, sewage, and fecal samples from various sources were examined for Rhodococcus coprophilus, associated actinomycetes, Escherichia coli, and fecal streptococci. Rhodococcus coprophilus was isolated consistently from feces of farm animals, poultry reared in proximity to farm animals, freshwater, and wastewater...

journal_title：Applied and environmental microbiology

authors： Mara DD,Oragui JI

更新日期：1981-12-01 00:00:00

abstract：:Soils harbor enormously diverse bacterial populations, and soil bacterial communities can vary greatly in composition across space. However, our understanding of the specific changes in soil bacterial community structure that occur across larger spatial scales is limited because most previous work has focused on eithe...

journal_title：Applied and environmental microbiology

authors： Lauber CL,Hamady M,Knight R,Fierer N

更新日期：2009-08-01 00:00:00

abstract：:An L-glutamine-overproducing mutant of an Escherichia coli K-12-derived strain was selected from randomly mutagenized cells in the course of L-alanyl-L-glutamine strain development. Genome-wide mutation analysis unveiled a novel mechanism for L-glutamine overproduction in this mutant. Three mutations were identified t...

journal_title：Applied and environmental microbiology

authors： Hayashi M,Tabata K

更新日期：2013-05-01 00:00:00

abstract：:Adsorption of Agrobacterium tumefaciens cells to potato tuber disks reached equilibrium after coincubation for about 30 min. More than 10% of the number of bacteria bound at equilibrium were adsorbed within 30 s. Adsorption isotherms obtained at three temperatures showed that the heat of adsorption was nearly zero. ...

journal_title：Applied and environmental microbiology

authors： Kluepfel DA,Pueppke SG

更新日期：1986-05-01 00:00:00

abstract：:Pyocins are bacteriocins secreted by Pseudomonas aeruginosa, and they assist in the colonization of different niches. A major subset of these antibacterial proteins adopt a modular organization characteristic of polymorphic toxins. They include a receptor-binding domain, a segment enabling membrane passage, and a toxi...

journal_title：Applied and environmental microbiology

authors： Ghequire MGK,Öztürk B

更新日期：2018-08-31 00:00:00

abstract：:Shuttle vectors that replicate stably and express selectable phenotypes in both Thermococcus kodakaraensis and Escherichia coli have been constructed. Plasmid pTN1 from Thermococcus nautilis was ligated to the commercial vector pCR2.1-TOPO, and selectable markers were added so that T. kodakaraensis transformants could...

journal_title：Applied and environmental microbiology

authors： Santangelo TJ,Cubonová L,Reeve JN

更新日期：2008-05-01 00:00:00

abstract：:Analysis of electron spin resonance spectra of 5-doxyl stearic acid in aqueous suspensions of Clostridium acetobutylicum ATCC 824 and the butanol-tolerant SA-2 derivative during a small-scale fermentation at three different butanol challenge levels indicated that the SA-2 strain is able to respond to the physical flui...

journal_title：Applied and environmental microbiology

authors： Baer SH,Bryant DL,Blaschek HP

更新日期：1989-10-01 00:00:00

abstract：:A Pseudomonas putida strain (MC4) that can utilize 2,3-dichloro-1-propanol (DCP) and several aliphatic haloacids and haloalcohols as sole carbon and energy source for growth was isolated from contaminated soil. Degradation of DCP was found to start with oxidation and concomitant dehalogenation catalyzed by a 72-kDa mo...

journal_title：Applied and environmental microbiology

authors： Arif MI,Samin G,van Leeuwen JG,Oppentocht J,Janssen DB

更新日期：2012-09-01 00:00:00