Prevalence of Escherichia coli O157 in Saskatchewan cattle: characterization of isolates by using random amplified polymorphic DNA PCR, antibiotic resistance profiles, and pathogenicity determinants.

abstract：:To observe real-time interactions between green fluorescent protein-labeled immune cells and invading bacteria in the zebrafish (Danio rerio), a series of plasmids was constructed for the red fluorescent protein (RFP) labeling of a variety of fish and human pathogens. The aim of this study was to create a collection o...

journal_title：Applied and environmental microbiology

authors： Singer JT,Phennicie RT,Sullivan MJ,Porter LA,Shaffer VJ,Kim CH

更新日期：2010-06-01 00:00:00

abstract：:Antialgal allelochemicals were isolated from Phragmites communis Tris. The isolated allelopathic fraction showed strong inhibition activity on the growth of Chlorella pyrenoidosa and Microcystis aeruginosa but had no inhibition on Chlorella vulgaris. The 50% effective concentrations (EC50) of the allelopathic fraction...

journal_title：Applied and environmental microbiology

authors： Li FM,Hu HY

更新日期：2005-11-01 00:00:00

abstract：:The role of Escherichia coli as a pathogen has been the focus of considerable study, while much less is known about it as a commensal and how it adapts to and colonizes different environmental niches within the mammalian gut. In this study, we characterize Escherichia coli organisms (n = 146) isolated from different r...

journal_title：Applied and environmental microbiology

authors： Abraham S,Gordon DM,Chin J,Brouwers HJ,Njuguna P,Groves MD,Zhang R,Chapman TA

更新日期：2012-10-01 00:00:00

abstract：:Staphylococcus aureus is a gram-positive pathogen that causes a variety of diseases, including bovine mastitis, which has severe economic consequences. Standard antibiotic treatment results in selection of resistant strains, leading to a need for alternative treatments, such as bacteriophage therapy. Forty-nine S. aur...

journal_title：Applied and environmental microbiology

authors： Synnott AJ,Kuang Y,Kurimoto M,Yamamichi K,Iwano H,Tanji Y

更新日期：2009-07-01 00:00:00

abstract：:The photosynthetic bacterium Rhodobacter sphaeroides is capable of producing H2 via nitrogenase when grown photoheterotrophically in the absence of N2. By using 14C-labeled malate, it was found that greater than 95% of this substrate was catabolized completely to CO2 during H2 production. About 60% of this catabolism ...

journal_title：Applied and environmental microbiology

authors： Macler BA,Bassham JA

更新日期：1988-11-01 00:00:00

abstract：:Fusarium tricinctum Fn-2B was grown on a rice substrate at room temperature (22 to 26 degrees C) for 2 weeks followed by growth at a low temperature (10 to 12 degrees C) for an additional 2 weeks. A total of 1.5 g of nivalenol and 80 mg of fusarenone-X were obtained from 2 kg of rice culture. The methods of production...

journal_title：Applied and environmental microbiology

authors： Lee YW,Mirocha CJ

更新日期：1984-10-01 00:00:00

abstract：:The endoglucanases of the thermophilic actinomycete Thermomonospora curvata were characterized. Early-exponential-phase culture fluid contained at least three endoglucanases, with molecular weights of 23,000, 46,000, and 146,000 and K(m) values of 1.54, 3.60, and 1.32% carboxymethyl cellulose, respectively. The statio...

journal_title：Applied and environmental microbiology

authors： Lupo D,Stutzenberger F

更新日期：1988-02-01 00:00:00

abstract：:A pathway was metabolically engineered to produce poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), a biodegradable thermoplastic with proven commercial applications, from a single, unrelated carbon source. An expression system was developed in which a prpC strain of Salmonella enterica serovar Typhimurium, with a ...

journal_title：Applied and environmental microbiology

authors： Aldor IS,Kim SW,Prather KL,Keasling JD

更新日期：2002-08-01 00:00:00

abstract：:We describe here a new enzyme-coupled assay for the quantitation of d-xylose using readily available enzymes that allows kinetic evaluation of hemicellulolytic enzymes using natural xylooligosaccharide substrates. Hydrogen peroxide is generated as an intermediary analyte, which allows flexibility in the choice of the ...

journal_title：Applied and environmental microbiology

authors： Wagschal K,Franqui-Espiet D,Lee CC,Robertson GH,Wong DW

更新日期：2005-09-01 00:00:00

abstract：:The outer membrane of microbial cells forms an effective barrier for hydrophobic compounds, potentially causing an uptake limitation for hydrophobic substrates. Low bioconversion activities (1.9 U g(cdw)(-1)) have been observed for the ω-oxyfunctionalization of dodecanoic acid methyl ester by recombinant Escherichia c...

journal_title：Applied and environmental microbiology

authors： Julsing MK,Schrewe M,Cornelissen S,Hermann I,Schmid A,Bühler B

更新日期：2012-08-01 00:00:00

abstract：:In this study, a number of Listeria monocytogenes strains of different origins were evaluated for in vitro invasion capacity for various human cell types (monocytic THP-1, enterocytic Caco-2, and hepatocytic HepG2 cells) and for expression levels of specific virulence genes. For THP-1 cells, no differences between cli...

journal_title：Applied and environmental microbiology

authors： Werbrouck H,Grijspeerdt K,Botteldoorn N,Van Pamel E,Rijpens N,Van Damme J,Uyttendaele M,Herman L,Van Coillie E

更新日期：2006-06-01 00:00:00

abstract：:Infectious salmon anemia (ISA) is a serious disease of marine-farmed Atlantic salmon (Salmo salar) caused by ISA virus (ISAV), belonging to the genus Isavirus, family Orthomyxoviridae. There is an urgent need to understand the virulence factors and pathogenic mechanisms of ISAV and to develop new vaccine approaches. U...

journal_title：Applied and environmental microbiology

authors： Toro-Ascuy D,Tambley C,Beltran C,Mascayano C,Sandoval N,Olivares E,Medina RA,Spencer E,Cortez-San Martín M

更新日期：2015-02-01 00:00:00

abstract：:Cyanase catalyzes the decomposition of cyanate into CO(2) and ammonium, with carbamate as an unstable intermediate. The cyanase of Pseudomonas pseudoalcaligenes CECT5344 was negatively regulated by ammonium and positively regulated by cyanate, cyanide, and some cyanometallic complexes. Cyanase activity was not detecte...

journal_title：Applied and environmental microbiology

authors： Luque-Almagro VM,Huertas MJ,Sáez LP,Luque-Romero MM,Moreno-Vivián C,Castillo F,Roldán MD,Blasco R

更新日期：2008-10-01 00:00:00

abstract：:The vertical and seasonal distribution and diversity of archaeal sequences was investigated in a hypersaline, stratified, monomictic lake, Solar Lake, Sinai, Egypt, during the limnological development of stratification and mixing. Archaeal sequences were studied via phylogenetic analysis of 16S rDNA sequences as well ...

journal_title：Applied and environmental microbiology

authors： Cytryn E,Minz D,Oremland RS,Cohen Y

更新日期：2000-08-01 00:00:00

abstract：:The bioconversion of waste paper to single-cell protein at pH <1 by Scytalidium acidophilum is described. Waste paper pretreated with 72% H(2)SO(4) at 4 degrees C was diluted with water to a pH of <0.1 and hydrolyzed. This yielded an adequate sugar-containing substrate for the growth of the fungus. A total of 97% of t...

journal_title：Applied and environmental microbiology

authors： Ivarson KC,Morita H

更新日期：1982-03-01 00:00:00

abstract：:Pseudomonas fluorescens isolates 13525 and 2-79 were grown in Luria broth and low-nutrient medium (LNM). Pililike fibrils were very rarely produced in Luria broth but were abundantly produced in LNM. In LNM the pili were peritrichously distributed and had diameters ranging from 3 to 8 nm. Pili were purified from strai...

journal_title：Applied and environmental microbiology

authors： Vesper SJ

更新日期：1987-07-01 00:00:00

abstract：:This is the first report of filamentous actinobacteria isolated from surface-sterilized root tissues of healthy wheat plants (Triticum aestivum L.). Wheat roots from a range of sites across South Australia were used as the source material for the isolation of the endophytic actinobacteria. Roots were surface-sterilize...

journal_title：Applied and environmental microbiology

authors： Coombs JT,Franco CM

更新日期：2003-09-01 00:00:00

abstract：:A computer program was developed to identify anaerobic bacteria by using simultaneous pattern recognition via a Bayesian probabilistic model. The system is intended for use as a rapid, precise, and reproducible aid in the identification of unknown isolates. The program operates on a data base of 28 genera comprising 2...

journal_title：Applied and environmental microbiology

authors： Kelley RW,Kellogg ST

更新日期：1978-03-01 00:00:00

abstract：:A d-xylose reductase mutant of Pachysolen tannophilus was isolated on the basis of its poor growth on d-xylose but normal growth on xylitol and d-glucose. Fractionation of cell extracts indicated that the mutant was deficient in d-xylose reductase activity that used NADPH exclusively as a cofactor, but not in activity...

journal_title：Applied and environmental microbiology

authors： Schneider H,Lee H,Barbosa Mde F,Kubicek CP,James AP

更新日期：1989-11-01 00:00:00

abstract：:The inability of two Lactobacillus strains to ferment D-xylose was complemented by the introduction of Lactobacillus pentosus genes encoding D-xylose isomerase, D-xylulose kinase, and a D-xylose catabolism regulatory protein. This result opens the possibility of using D-xylose fermentation as a food-grade selection ma...

journal_title：Applied and environmental microbiology

authors： Posno M,Heuvelmans PT,van Giezen MJ,Lokman BC,Leer RJ,Pouwels PH

更新日期：1991-09-01 00:00:00

abstract：:Lactobacillus gasseri ATCC 33323, Lactobacillus salivarius subsp. salivarius UCC 118, and Lactobacillus casei ATCC 334 contain one (LgaI), four (Sal1, Sal2, Sal3, Sal4), and one (Lca1) distinguishable prophage sequences, respectively. Sequence analysis revealed that LgaI, Lca1, Sal1, and Sal2 prophages belong to the g...

journal_title：Applied and environmental microbiology

authors： Ventura M,Canchaya C,Bernini V,Altermann E,Barrangou R,McGrath S,Claesson MJ,Li Y,Leahy S,Walker CD,Zink R,Neviani E,Steele J,Broadbent J,Klaenhammer TR,Fitzgerald GF,O'toole PW,van Sinderen D

更新日期：2006-05-01 00:00:00

abstract：:An assembly consisting of Styrofoam cups with membranes of varying porosities was developed to study microorganism-root interactions. The assembly permitted uniform distribution of a bacterium in soil and was simple, easy to use, and disposable. In tests with the bacterium Pseudomonas solanacearum, little difference i...

journal_title：Applied and environmental microbiology

authors： Hartel PG,Billingsley JW,Williamson JW

更新日期：1989-05-01 00:00:00

abstract：:The host range and transfer frequency of an IncP-1 plasmid (pKJK10) among indigenous bacteria in the barley rhizosphere was investigated. A new flow cytometry-based cultivation-independent method for enumeration and sorting of transconjugants for subsequent 16S rRNA gene classification was used. Indigenous transconjug...

journal_title：Applied and environmental microbiology

authors： Musovic S,Oregaard G,Kroer N,Sørensen SJ

更新日期：2006-10-01 00:00:00

abstract：:Here, we report the genome of one gammaproteobacterial member of the gut microbiota, for which we propose the name "Candidatus Schmidhempelia bombi," that was inadvertently sequenced alongside the genome of its host, the bumble bee, Bombus impatiens. This symbiont is a member of the recently described bacterial order ...

journal_title：Applied and environmental microbiology

authors： Martinson VG,Magoc T,Koch H,Salzberg SL,Moran NA

更新日期：2014-07-01 00:00:00

abstract：:A field evaluation of double violet agar for the isolation and presumptive identification of Klebsiella pneumoniae from water has been performed. Water from the North Oconee River, Clarke County, Ga., was cultured for presence of klebsiellae using the membrane filter technique. Colonies were presumptively identified a...

journal_title：Applied and environmental microbiology

authors： Campbell LM,Roth IL,Klein RD

更新日期：1976-02-01 00:00:00

abstract：:The maximum growth temperature, the optimal growth temperature, and the estimated normal physiological range for growth of Shewanella gelidimarina are functions of water activity (a(w)), which can be manipulated by changing the concentration of sodium chloride. The growth temperatures at the boundaries of the normal p...

journal_title：Applied and environmental microbiology

authors： Nichols DS,Olley J,Garda H,Brenner RR,McMeekin TA

更新日期：2000-06-01 00:00:00

abstract：:Pierce's disease is a devastating lethal disease of Vitus vinifera grapevines caused by the bacterium Xylella fastidiosa. There is no cure for Pierce's disease, and control is achieved predominantly by suppressing transmission of the glassy-winged sharpshooter insect vector. We present a simple robust approach for the...

journal_title：Applied and environmental microbiology

authors： Azizi A,Arora A,Markiv A,Lampe DJ,Miller TA,Kang AS

更新日期：2012-04-01 00:00:00

abstract：:The influence of physiological and environmental factors on the accumulation of oxindole during anaerobic indole metabolism was investigated by high-performance liquid chromatography. Under methanogenic conditions, indole was temporarily converted to oxindole in stoichiometric amounts in media inoculated with three fr...

journal_title：Applied and environmental microbiology

authors： Madsen EL,Francis AJ,Bollag JM

更新日期：1988-01-01 00:00:00

abstract：:Campylobacter infections are increasing and pose a serious public health problem in Denmark. Infections in humans and broiler flocks show similar seasonality, suggesting that climate may play a role in infection. We examined the effects of temperature, precipitation, relative humidity, and hours of sunlight on Campylo...

journal_title：Applied and environmental microbiology

authors： Patrick ME,Christiansen LE,Wainø M,Ethelberg S,Madsen H,Wegener HC

更新日期：2004-12-01 00:00:00

abstract：:Respiratory denitrification is not always adequately established when bacteria are characterized. We have tested a simple method that allows one to evaluate whether the two necessary criteria to claim denitrification have been met, namely, that N(inf2) or N(inf2)O is produced from nitrate or nitrite and that this redu...

journal_title：Applied and environmental microbiology

authors： Mahne I,Tiedje JM

更新日期：1995-03-01 00:00:00