Complementation of the inability of Lactobacillus strains to utilize D-xylose with D-xylose catabolism-encoding genes of Lactobacillus pentosus.

abstract：:We used an inverse metabolic engineering approach to identify gene targets for improved xylose assimilation in recombinant Saccharomyces cerevisiae. Specifically, we created a genomic fragment library from Pichia stipitis and introduced it into recombinant S. cerevisiae expressing XYL1 and XYL2. Through serial subcult...

journal_title：Applied and environmental microbiology

authors： Jin YS,Alper H,Yang YT,Stephanopoulos G

更新日期：2005-12-01 00:00:00

abstract：:Bifidobacterium and Lactobacillus can beneficially affect the host by producing acetic acid and lactic acid, which lower pH and thereby inhibit the growth of pathogens or allow the probiotic bacteria to compete with pathogens for epithelial adhesion sites and nutrients. The transmural migration of enteric organisms in...

journal_title：Applied and environmental microbiology

authors： Wang IK,Lai HC,Yu CJ,Liang CC,Chang CT,Kuo HL,Yang YF,Lin CC,Lin HH,Liu YL,Chang YC,Wu YY,Chen CH,Li CY,Chuang FR,Huang CC,Lin CH,Lin HC

更新日期：2012-02-01 00:00:00

abstract：:The following two factors significantly influence estimates of the maximum specific growth rate ( micro (max)) and the lag-phase duration (lambda): (i) the technique used to monitor bacterial growth and (ii) the model fitted to estimate parameters. In this study, nine strains of Listeria monocytogenes were monitored s...

journal_title：Applied and environmental microbiology

authors： Baty F,Flandrois JP,Delignette-Muller ML

更新日期：2002-12-01 00:00:00

abstract：:One fluorescent compound previously named TDP-2 was isolated and purified from a rice culture of Fusarium equiseti (Alaska 2-2). Mass spectral and nuclear magnetic resonance data indicated that it is a C-3'-N-acetyl derivative of fusarochromanone, a newly discovered mycotoxin. Time course studies of synthesis of these...

journal_title：Applied and environmental microbiology

authors： Xie WP,Mirocha CJ,Pawlosky RJ,Wen YC,Xu XG

更新日期：1989-04-01 00:00:00

abstract：:Previous studies have shown that the cellobiohydrolases of the white rot basidiomycete Phanerochaete chrysosporium are encoded by a family of structurally related genes. In this investigation, we identified and sequenced the most highly transcribed gene, cbh1-4. Evidence suggests that in this fungus the dominant isozy...

journal_title：Applied and environmental microbiology

authors： Vanden Wymelenberg A,Covert S,Cullen D

更新日期：1993-10-01 00:00:00

abstract：:A fragment of about 500 base pairs of the beta-hemolysin gene from Listeria monocytogenes was used to screen different bacterial strains by DNA colony hybridization. The cells in the colonies were lysed by microwaves in the presence of sodium hydroxide. Of 52 different strains of Listeria species screened, only the DN...

journal_title：Applied and environmental microbiology

authors： Datta AR,Wentz BA,Hill WE

更新日期：1987-09-01 00:00:00

abstract：:Growth, survival, and pathogenicity of Klebsiella growing in and on environmental foci were examined. Total coliforms present in raw wastes from pulp mills were in excess of 10(5)/ml, and 60 to 80% were Klebsiella. Fecal coliform counts ranged from 10(1) to 10(5)/ml. Klebsiella isolates from industrial effluents and a...

journal_title：Applied and environmental microbiology

authors： Knittel MD,Seidler RJ,Eby C,Cabe LM

更新日期：1977-11-01 00:00:00

abstract：:Pseudomonas putida KT2440, a saprophytic soil bacterium that colonizes the plant root, is a suitable microorganism for the removal of pollutants and a stable host for foreign genes used in biotransformation processes. Because of its potential use in agriculture and industry, we investigated the conditions for the opti...

journal_title：Applied and environmental microbiology

authors： Muñoz-Rojas J,Bernal P,Duque E,Godoy P,Segura A,Ramos JL

更新日期：2006-01-01 00:00:00

abstract：:Pfiesteria piscicida is a heterotrophic dinoflagellate widely distributed along the middle Atlantic shore of the United States and associated with fish kills in the Neuse River (North Carolina) and the Chesapeake Bay (Maryland and Virginia). We constructed a genomic DNA library from clonally cultured P. piscicida and ...

journal_title：Applied and environmental microbiology

authors： Saito K,Drgon T,Robledo JA,Krupatkina DN,Vasta GR

更新日期：2002-11-01 00:00:00

abstract：:Stromatolites, organosedimentary structures formed by microbial activity, are found throughout the geological record and are important markers of biological history. More conspicuous in the past, stromatolites occur today in a few shallow marine environments, including Hamelin Pool in Shark Bay, Western Australia. Ham...

journal_title：Applied and environmental microbiology

authors： Papineau D,Walker JJ,Mojzsis SJ,Pace NR

更新日期：2005-08-01 00:00:00

abstract：:Methyl fluoride is frequently used to specifically inhibit acetoclastic methanogenesis, thus allowing determination of the relative contribution of acetate versus H2/CO2 to total CH4 production in natural environments. However, the effect of the inhibitor on growth of the target archaeal population has not yet been st...

journal_title：Applied and environmental microbiology

authors： Penning H,Conrad R

更新日期：2006-01-01 00:00:00

abstract：:Organic matter in sewage, soil, and aquatic systems may enhance or inhibit the infectivity of viruses associated with particulates (e.g., clay minerals, sediments). The purpose of this investigation was to identify the mechanisms whereby organic matter, in the form of defined proteins, affects the adsorption of reovir...

journal_title：Applied and environmental microbiology

authors： Lipson SM,Stotzky G

更新日期：1984-09-01 00:00:00

abstract：:Colonization of apple leaves by the yeastlike fungus Aureobasidium pullulans was followed quantitatively and spatially at a microscale level throughout two growing seasons. Ten field leaves were sampled on 11 dates in 2003 and 15 dates in 2004. Using an A. pullulans-specific fluorescence in situ hybridization probe an...

journal_title：Applied and environmental microbiology

authors： McGrath MJ,Andrews JH

更新日期：2006-09-01 00:00:00

abstract：:LeLoir glycosyltransferases are important biocatalysts for the production of glycosidic bonds in natural products, chiral building blocks, and pharmaceuticals. Trehalose transferase (TreT) is of particular interest since it catalyzes the stereo- and enantioselective α,α-(1→1) coupling of a nucleotide sugar donor and m...

journal_title：Applied and environmental microbiology

authors： Mestrom L,Marsden SR,Dieters M,Achterberg P,Stolk L,Bento I,Hanefeld U,Hagedoorn PL

更新日期：2019-04-04 00:00:00

abstract：:Pure cultures of Escherichia coli and Streptococcus faecalis and environmental water samples were examined for the possibility that pollution involving organotin compounds could decrease the values for indicator organisms when standard methods were applied to the analysis of water samples. (CH3)2SnCl2 and (CH3)3SnCl d...

journal_title：Applied and environmental microbiology

authors： Pettibone GW,Cooney JJ

更新日期：1986-09-01 00:00:00

abstract：:Each year, thousands of new protistan 18S rRNA sequences are detected in environmental samples. Many of these sequences are molecular signatures of new protistan species, classes, and/or kingdoms that have never been seen before. The main goal of this study was to enable visualization of these novel organisms and to c...

journal_title：Applied and environmental microbiology

authors： Stoeck T,Fowle WH,Epstein SS

更新日期：2003-11-01 00:00:00

abstract：:[This corrects the article on p. 1301 in vol. 50.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1986-03-01 00:00:00

abstract：:The association between grass roots and Azospirillum brasilense Sp 7 was investigated by the Fahraeus slide technique, using nitrogen-free medium. Young inoculated roots of pearl millet and guinea grass produced more mucilaginous sheath (mucigel), root hairs, and lateral roots than did uninoculated sterile controls. T...

journal_title：Applied and environmental microbiology

authors： Umali-Garcia M,Hubbell DH,Gaskins MH,Dazzo FB

更新日期：1980-01-01 00:00:00

abstract：:A device for sampling at depths of up to 6,000 m is described in which 3 liters of seawater is concentrated over a Nucleopore filter to about 13 ml and retrieved under in situ pressure and temperature. Subsamples can be withdrawn into transfer units that are equipped with individual gas accumulators for preventing los...

journal_title：Applied and environmental microbiology

authors： Jannasch HW,Wirsen CO

更新日期：1977-03-01 00:00:00

abstract：:The population dynamics of bifidobacteria in human feces during raffinose administration were investigated at the species level by using fluorescence in situ hybridization (FISH) coupled with flow cytometry (FCM) analysis. Although double-staining FISH-FCM using both fluorescein isothiocyanate (FITC) and indodicarbocy...

journal_title：Applied and environmental microbiology

authors： Dinoto A,Marques TM,Sakamoto K,Fukiya S,Watanabe J,Ito S,Yokota A

更新日期：2006-12-01 00:00:00

abstract：:A separation procedure for the analysis of the enzyme components of the hyperthermophilic bacterium Thermotoga maritima involved in cellulose and xylan degradation was developed. Resolution of the enzymes was achieved by a combination of fast protein liquid chromatography anion exchange and hydrophobic interaction chr...

journal_title：Applied and environmental microbiology

authors： Bronnenmeier K,Kern A,Liebl W,Staudenbauer WL

更新日期：1995-04-01 00:00:00

abstract：:The only major structural protein (35 kDa) of the lactococcal small isometric-headed bacteriophage ul36, a member of the P335 species, was isolated from a preparative sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. Monoclonal antibodies (MAbs) were raised against the denatured 35-kDa protein. Six MAbs...

journal_title：Applied and environmental microbiology

authors： Moineau S,Bernier D,Jobin M,Hébert J,Klaenhammer TR,Pandian S

更新日期：1993-07-01 00:00:00

abstract：:Intact core tetraether membrane lipids of marine planktonic Crenarchaeota were quantified in water column-suspended particulate matter obtained from four depth intervals ( approximately 70, 500, 1,000 and 1,500 m) at seven stations in the northwestern Arabian Sea to investigate the distribution of the organisms at var...

journal_title：Applied and environmental microbiology

authors： Sinninghe Damsté JS,Rijpstra WI,Hopmans EC,Prahl FG,Wakeham SG,Schouten S

更新日期：2002-06-01 00:00:00

abstract：:Indoxyl-beta-D-glucuronide (indoxyl) was evaluated as a specific chromogen for detection of Escherichia coli by the membrane filter method. In all, 413 colonies were tested from the indoxyl-supplemented media, yielding 93.3% confirmation, as E. coli. Compared with the indoxyl medium, other media gave either much lower...

journal_title：Applied and environmental microbiology

authors： Haines JR,Covert TC,Rankin CC

更新日期：1993-08-01 00:00:00

abstract：:The hydroaromatic acids shikimate and quinate, which may be available as carbon sources in the soil, supported production of only low levels of acidic exopolysaccharide by Bradyrhizobium japonicum. Exopolysaccharide production (micrograms per 10 cells) was 4.9 on quinate and 4.5 on shikimate; in comparison, it was 128...

journal_title：Applied and environmental microbiology

authors： Tully RE

更新日期：1988-06-01 00:00:00

abstract：:A simplified X-ray film method for detecting bacterial production of Hgo by Escherichia coli was developed. There was excellent agreement of results between this X-ray film method and radioactive mercury volatilization assays. Although not quantitative, the X-ray film method has the advantages of being rapid, simple t...

journal_title：Applied and environmental microbiology

authors： Nakamura K,Nakahara H

更新日期：1988-11-01 00:00:00

abstract：:A fixed-bed loop, high-rate anaerobic bioreactor treating sulfite evaporator condensate was sampled when it reached steady state and afterwards following perturbations during a 14-month period. By using immunotechnology, it was observed that shifts in methanogenic subpopulations occurred in association with perturbati...

journal_title：Applied and environmental microbiology

authors： Macario AJ,Conway de Macario E,Ney U,Schoberth SM,Sahm H

更新日期：1989-08-01 00:00:00

abstract：:Aphanothece halophytica is a halotolerant alkaliphilic cyanobacterium which can grow at NaCl concentrations up to 3.0 M and at pH values up to 11. The genome sequence revealed that the cyanobacterium Synechocystis sp. strain PCC 6803 contains five putative Na+/H+ antiporters, two of which are homologous to NhaP of Pse...

journal_title：Applied and environmental microbiology

authors： Wutipraditkul N,Waditee R,Incharoensakdi A,Hibino T,Tanaka Y,Nakamura T,Shikata M,Takabe T,Takabe T

更新日期：2005-08-01 00:00:00

abstract：:The rhizobial bacterium Bradyrhizobium japonicum functions as a nitrogen-fixing symbiont of the soybean plant (Glycine max). Plants are capable of producing an oxidative burst, a rapid proliferation of reactive oxygen species (ROS), as a defense mechanism against pathogenic and symbiotic bacteria. Therefore, B. japoni...

journal_title：Applied and environmental microbiology

authors： Donati AJ,Jeon JM,Sangurdekar D,So JS,Chang WS

更新日期：2011-06-01 00:00:00

abstract：:The bacterium Rhodopseudomonas palustris grows with the aromatic acid benzoate and the alicyclic acid cyclohexanecarboxylate (CHC) as sole carbon sources. The enzymatic steps in an oxygen-independent pathway for CHC degradation have been elucidated, but it was unknown how the CHC operon (badHI aliAB badK) encoding the...

journal_title：Applied and environmental microbiology

authors： Hirakawa H,Hirakawa Y,Greenberg EP,Harwood CS

更新日期：2015-07-01 00:00:00