Environmental factors affecting indole metabolism under anaerobic conditions.

abstract：:Azospirillum lipoferum 4T has original properties such as nonmotility, melanin synthesis, and laccase activity. Following random Tn5 mutagenesis in A. lipoferum 4T, we obtained 10 mutants which were affected in melanization and laccase activity. The class 1 mutants, with intermediate levels of laccase activity, showed...

journal_title：Applied and environmental microbiology

authors： Faure D,Bouillant ML,Bally R

更新日期：1994-09-01 00:00:00

abstract：:Several variations in the scintillation mixture and the filter paper arrangements for double-vial radiorespirometry were compared. Improved efficiencies (44%) and shorter response times were found by adding wetting agents and methanolic NaOH to the scintillation mixture in the filter paper. The scintillation chemicals...

journal_title：Applied and environmental microbiology

authors： McKinley VL,Federle TW,Vestal JR

更新日期：1983-01-01 00:00:00

abstract：:The human gut is host to a diversity of microorganisms, including the single-celled microbial eukaryote Blastocystis. Research has shown that most carriers host a single Blastocystis subtype (ST), which is unusual given the considerable within-host species diversity observed for other microbial genera in this ecosyste...

journal_title：Applied and environmental microbiology

authors： Scanlan PD,Stensvold CR,Cotter PD

更新日期：2015-06-15 00:00:00

abstract：:An enzyme-linked immunosorbent assay was developed for the detection of whole cells of methanogens in samples from anaerobic continuously stirred tank digesters treating slurries of solid waste. The assay was found to allow for quantitative analysis of the most important groups of methanogens in samples from anaerobic...

journal_title：Applied and environmental microbiology

authors： Sørensen AH,Ahring BK

更新日期：1997-05-01 00:00:00

abstract：:Legionella pneumophila was detected and identified by an immunoblot assay using a monoclonal antibody specific to serogroups 1 to 8. Samples containing L. pneumophila were plated on buffered charcoal yeast extract agar supplemented with glycine, vancomycin, and polymyxin B. After incubation at 35 degrees C for 3 days,...

journal_title：Applied and environmental microbiology

authors： Bérubé A,Trudel M,Payment P

更新日期：1989-06-01 00:00:00

abstract：:Aspergillus niger is known for its efficient excretion machinery. However, problems have often arisen in obtaining high amounts of heterologous proteins in the culture medium. Here we present a quick method using sandwiched colonies to evaluate transgenic strains for secretion of heterologous proteins. Expressing the ...

journal_title：Applied and environmental microbiology

authors： Asgeirsdóttir SA,Scholtmeijer K,Wessels JG

更新日期：1999-05-01 00:00:00

abstract：:A new design, flat-bottom combination pH electrode was evaluated for utility in determining the pH of prepared agar media by surface contact of the electrode with the agar media. ...

journal_title：Applied and environmental microbiology

authors： Gorski TW,Ritzert RW

更新日期：1977-08-01 00:00:00

abstract：:In this study, multilocus sequence typing (MLST) was combined with the genetic detection of six genetic markers, ansB, dmsA, ggt, cj1585c, cjj81176-1367/71 (cj1365c), and the two-gene marker tlp7 (cj0951c plus cj0952c), to assess if their presence correlated with different C. jejuni clonal groups. Using a collection o...

journal_title：Applied and environmental microbiology

authors： Zautner AE,Herrmann S,Corso J,Tareen AM,Alter T,Gross U

更新日期：2011-04-01 00:00:00

abstract：:A 1.5-kb region immediately downstream of the styABCD operon involved in styrene degradation in Pseudomonas putida CA-3 has been cloned. Sequence analysis revealed a 1,296-bp open reading frame, designated styE, and BLAST P database comparisons of the deduced StyE amino acid sequence revealed 33 to 98% identity with s...

journal_title：Applied and environmental microbiology

authors： Mooney A,O'Leary ND,Dobson AD

更新日期：2006-02-01 00:00:00

abstract：:Laccases couple the oxidation of phenolic compounds to the reduction of molecular oxygen and thus span a wide variety of applications. While laccases of eukaryotes and bacteria are well characterized, these enzymes have not been described in archaea. Here, we report the purification and characterization of a laccase (...

journal_title：Applied and environmental microbiology

authors： Uthandi S,Saad B,Humbard MA,Maupin-Furlow JA

更新日期：2010-02-01 00:00:00

abstract：:Epifluorescent microscopy and flow cytometry were used in different combinations with fluorescein isothiocyanate-labeled immunoglobulins M and G3 to estimate the numbers of Cryptosporidium parvum oocysts in soil extracts containing 10 to 10,017 oocysts/ml. No combination had a systematic effect on accuracy or precisio...

journal_title：Applied and environmental microbiology

authors： Walker M,Redelman D

更新日期：2004-03-01 00:00:00

abstract：:VanA-type human (n=69), animal (n=49), and food (n=36) glycopeptide-resistant enterococci (GRE) from different geographic areas were investigated to study their possible reservoirs and transmission routes. Pulsed-field gel electrophoresis (PFGE) revealed two small genetically related clusters, M39 (n=4) and M49 (n=13)...

journal_title：Applied and environmental microbiology

authors： Biavasco F,Foglia G,Paoletti C,Zandri G,Magi G,Guaglianone E,Sundsfjord A,Pruzzo C,Donelli G,Facinelli B

更新日期：2007-05-01 00:00:00

abstract：:The methylotrophic yeast Pichia pastoris is widely used for the expression of heterologous enzymes. While the purity of the desired expression product is of major importance for many applications, we found that recombinant enzymes produced in methanol medium were contaminated by a 37-kDa endogenous yeast protease. Thi...

journal_title：Applied and environmental microbiology

authors： Salamin K,Sriranganadane D,Léchenne B,Jousson O,Monod M

更新日期：2010-07-01 00:00:00

abstract：:By using an autoradiographic technique, one can follow, during ripening of a cheese, the distribution, size, and metabolic activity of microcolonies. Fragments of cheese were labeled with [H]leucine, fixed, and mounted in epoxy resin. After exposure and development, sections were examined by optical microscopy. In Cam...

journal_title：Applied and environmental microbiology

authors： Reyrolle J,Letellier F

更新日期：1979-12-01 00:00:00

abstract：:Severe intoxications caused by the Bacillus cereus emetic toxin cereulide can hardly be prevented due to the ubiquitous distribution and heat resistance of spores and the extreme thermal and chemical stability of cereulide. It would therefore be desirable to inhibit cereulide synthesis during food manufacturing proces...

journal_title：Applied and environmental microbiology

authors： Frenzel E,Letzel T,Scherer S,Ehling-Schulz M

更新日期：2011-02-01 00:00:00

abstract：:Large numbers of bacteriophages (2 x 10(7) to 1 x 10(8)/ml) were present in ruminal fluid from sheep and cattle. Twenty-six distinct types were identified and placed in three morphological groups; several phages possessed unusual structural features. The large numbers and diversity of phages observed indicates a possi...

journal_title：Applied and environmental microbiology

authors： Klieve AV,Bauchop T

更新日期：1988-06-01 00:00:00

abstract：:A selective and differential growth medium was developed for detection of Clostridium botulinum types A, B, and F. The medium consisted of peptone-glucose-yeast extract agar supplemented with cycloserine, 250 micrograms/ml; sulfamethoxazole, 76 micrograms/ml; and trimethoprim, 4 micrograms/ml as selective inhibitors a...

journal_title：Applied and environmental microbiology

authors： Silas JC,Carpenter JA,Hamdy MK,Harrison MA

更新日期：1985-10-01 00:00:00

abstract：:Survival studies were conducted on Legionella pneumophila cells that had been grown intracellularly in Acanthamoeba polyphaga and then exposed to polyhexamethylene biguanide (PHMB), benzisothiazolone (BIT), and 5-chloro-N-methylisothiazolone (CMIT). Susceptibilities were also determined for L. pneumophila grown under ...

journal_title：Applied and environmental microbiology

authors： Barker J,Brown MR,Collier PJ,Farrell I,Gilbert P

更新日期：1992-08-01 00:00:00

abstract：:Two hybrid bacteriocins, enterocin E50-52/pediocin PA-1 (EP) and pediocin PA-1/enterocin E50-52 (PE), were designed by combining the N terminus of enterocin E50-52 and the C terminus of pediocin PA-1 and by combining the C terminus of pediocin PA-1 and the N terminus of enterocin E50-52, respectively. Both hybrid bact...

journal_title：Applied and environmental microbiology

authors： Tiwari SK,Sutyak Noll K,Cavera VL,Chikindas ML

更新日期：2015-03-01 00:00:00

abstract：:Different nutrient receptors varied in triggering germination of Bacillus subtilis spores with a pressure of 150 MPa, the GerA receptor being more responsive than the GerB receptor and even more responsive than the GerK receptor. This hierarchy in receptor responsiveness to pressure was the same as receptor responsive...

journal_title：Applied and environmental microbiology

authors： Black EP,Koziol-Dube K,Guan D,Wei J,Setlow B,Cortezzo DE,Hoover DG,Setlow P

更新日期：2005-10-01 00:00:00

abstract：:We examined the influence of elevated CO2 concentration on denitrifier enzyme activity in wheat rhizoplanes by using controlled environments and solution culture techniques. Potential denitrification activity was from 3 to 24 times higher on roots that were grown under an elevated CO2 concentration of 1,000 micromoles...

journal_title：Applied and environmental microbiology

authors： Smart DR,Ritchie K,Stark JM,Bugbee B

更新日期：1997-11-01 00:00:00

abstract：:The genes encoding thermostable alpha- and beta-galactosidases from an extremely thermophilic bacterium, Thermus strain T2, were cloned in Escherichia coli. The alpha-galactosidase gene was located just downstream from the beta-galactosidase gene. The genes were introduced into Thermus thermophilus HB27 with the aid o...

journal_title：Applied and environmental microbiology

authors： Koyama Y,Okamoto S,Furukawa K

更新日期：1990-07-01 00:00:00

abstract：:Electrorotation is a noninvasive technique that is capable of detecting changes in the morphology and physicochemical properties of microorganisms. Electrorotation studies are reported for two intestinal parasites, Giardia intestinalis and Cyclospora cayetanensis. It is concluded that viable and nonviable G. intestina...

journal_title：Applied and environmental microbiology

authors： Dalton C,Goater AD,Pethig R,Smith HV

更新日期：2001-02-01 00:00:00

abstract：:Digital dermatitis (DD) is an important cause of lameness in dairy cattle worldwide. It has now been reported in beef cattle and also sheep (contagious ovine digital dermatitis [CODD]). Three Treponema phylogroups are consistently isolated from lesions, Treponema medium-like, Treponema phagedenis-like, and Treponema p...

journal_title：Applied and environmental microbiology

authors： Sullivan LE,Carter SD,Duncan JS,Grove-White DH,Angell JW,Evans NJ

更新日期：2015-11-01 00:00:00

abstract：:To examine whether the heat-labile enterotoxin gene in porcine enterotoxigenic Escherichia coli (ETEC) strains is as divergent as in human ETEC strains, we sequenced the heat-labile and heat-stable toxin genes from 52 and 33 porcine ETEC strains, respectively. We found that the STa gene is identical, that the LT gene ...

journal_title：Applied and environmental microbiology

authors： Zhang C,Rausch D,Zhang W

更新日期：2009-10-01 00:00:00

abstract：:A gram-negative bacterium isolated from activated sludge was able to utilize up to 25 mM phosphonoacetate as the sole carbon and phosphorus source, with simultaneous excretion of virtually equimolar levels of phosphate. 2-Aminoethylphosphonate was similarly utilized with equivalent growth rates and cellular yields, wh...

journal_title：Applied and environmental microbiology

authors： McMullan G,Harrington F,Quinn JP

更新日期：1992-04-01 00:00:00

abstract：:An important feature of microbial biofilms is the development of four-dimensional physical and chemical gradients in space and time. There is need for novel approaches to probe these so-called microenvironments to determine their effect on biofilm-specific processes. In this study, we describe the use of seminaphthorh...

journal_title：Applied and environmental microbiology

authors： Hunter RC,Beveridge TJ

更新日期：2005-05-01 00:00:00

abstract：:Lipopolysaccharides (LPS) were isolated from four species of cyanobacteria (Anabaena flos-aquae UTEX 1444. A. cylindrica, Oscillatoria tenuis, and O. brevis) frequently occurring in drinking-water supplies. The cyanobacterial LPS contained glucose, xylose, mannose, and rhamnose, but differed from the LPS derived from ...

journal_title：Applied and environmental microbiology

authors： Keleti G,Sykora JL

更新日期：1982-01-01 00:00:00

abstract：:The distribution of methanogenic and sulfate-reducing bacteria was examined in sediments from three sites off the coast of eastern Connecticut and five sites in Long Island Sound. Both bacterial groups were detected at all sites. Three distributional patterns were observed: (i) four sites exhibited methanogenic and su...

journal_title：Applied and environmental microbiology

authors： Hines ME,Buck JD

更新日期：1982-02-01 00:00:00

abstract：:The antifungal protein AFP from Aspergillus giganteus is highly effective in restricting the growth of major human- and plant-pathogenic filamentous fungi. However, a fundamental prerequisite for the use of AFP as an antifungal drug is a complete understanding of its mode of action. In this study, we performed several...

journal_title：Applied and environmental microbiology

authors： Hagen S,Marx F,Ram AF,Meyer V

更新日期：2007-04-01 00:00:00