Viability of Giardia intestinalis cysts and viability and sporulation state of Cyclospora cayetanensis oocysts determined by electrorotation.

Abstract:

:Electrorotation is a noninvasive technique that is capable of detecting changes in the morphology and physicochemical properties of microorganisms. Electrorotation studies are reported for two intestinal parasites, Giardia intestinalis and Cyclospora cayetanensis. It is concluded that viable and nonviable G. intestinalis cysts can be differentiated by this technique, and support for this conclusion was obtained using a fluorogenic vital dye assay and morphological indicators. The viability of C. cayetanensis oocysts (for which no vital dye assay is currently available) can also be determined by electrorotation, as can their sporulation state. Modeling of the electrorotational response of these organisms was used to determine their dielectric properties and to gain an insight into the changes occurring within them. Electrorotation offers a new, simple, and rapid method for determining the viability of parasites in potable water and food products and as such has important healthcare implications.

journal_name

Appl Environ Microbiol

authors

Dalton C,Goater AD,Pethig R,Smith HV

doi

10.1128/AEM.67.2.586-590.2001

keywords:

subject

Has Abstract

pub_date

2001-02-01 00:00:00

pages

586-90

issue

2

eissn

0099-2240

issn

1098-5336

journal_volume

67

pub_type

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