Biomineralization of uranium by PhoY phosphatase activity aids cell survival in Caulobacter crescentus.

Abstract:

:Caulobacter crescentus is known to tolerate high levels of uranium [U(VI)], but its detoxification mechanism is poorly understood. Here we show that C. crescentus is able to facilitate U(VI) biomineralization through the formation of U-Pi precipitates via its native alkaline phosphatase activity. The U-Pi precipitates, deposited on the cell surface in the form of meta-autunite structures, have a lower U/Pi ratio than do chemically produced precipitates. The enzyme that is responsible for the phosphatase activity and thus the biomineralization process is identified as PhoY, a periplasmic alkaline phosphatase with broad substrate specificity. Furthermore, PhoY is shown to confer a survival advantage on C. crescentus toward U(VI) under both growth and nongrowth conditions. Results obtained in this study thus highlight U(VI) biomineralization as a resistance mechanism in microbes, which not only improves our understanding of bacterium-mineral interactions but also aids in defining potential ecological niches for metal-resistant bacteria.

journal_name

Appl Environ Microbiol

authors

Yung MC,Jiao Y

doi

10.1128/AEM.01050-14

subject

Has Abstract

pub_date

2014-08-01 00:00:00

pages

4795-804

issue

16

eissn

0099-2240

issn

1098-5336

pii

AEM.01050-14

journal_volume

80

pub_type

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