Environmental dependence of stationary-phase metabolism in Bacillus subtilis and Escherichia coli.

abstract：:The use of Lactococcus lactis (the most extensively characterized lactic acid bacterium) as a delivery organism for heterologous proteins is, in some cases, limited by low production levels and poor-quality products due to surface proteolysis. In this study, we combined in one L. lactis strain use of the nisin-inducib...

journal_title：Applied and environmental microbiology

authors： Miyoshi A,Poquet I,Azevedo V,Commissaire J,Bermudez-Humaran L,Domakova E,Le Loir Y,Oliveira SC,Gruss A,Langella P

更新日期：2002-06-01 00:00:00

abstract：:Production of propionicin PLG-1 by Propionibacterium thoenii P127 was pH dependent, with maximal activity detected in supernatants of cultures grown at pH 7.0 Propionicin PLG-1 was purified by ion-exchange chromatography and isoelectric focusing. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of propionicin...

journal_title：Applied and environmental microbiology

authors： Lyon WJ,Glatz BA

更新日期：1993-01-01 00:00:00

abstract：:This report demonstrates the applicability of a combination of matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) and chemometrics for rapid and reliable identification of vegetative cells of the causative agent of anthrax, Bacillus anthracis. Bacillus cultures were prepared ...

journal_title：Applied and environmental microbiology

authors： Lasch P,Beyer W,Nattermann H,Stämmler M,Siegbrecht E,Grunow R,Naumann D

更新日期：2009-11-01 00:00:00

abstract：:It has been hypothesized that the environment can influence the composition of the nasal microbiota. However, the direct influence of pig farming on the anterior and posterior nasal microbiota is unknown. Using a cross-sectional design, pig farms (n = 28) were visited in 2014 to 2015, and nasal swabs from 43 pig farme...

journal_title：Applied and environmental microbiology

authors： Kraemer JG,Ramette A,Aebi S,Oppliger A,Hilty M

更新日期：2018-03-01 00:00:00

abstract：:A procedure was developed to isolate and determine ergot alkaloid production by Acremonium coenophialum, the endophytic fungus of tall fescue. The procedure established that macerated leaf sheath or pith from inflorescence stem placed either in a liquid medium or on a corn meal-malt extract agar medium produced isolat...

journal_title：Applied and environmental microbiology

authors： Bacon CW

更新日期：1988-11-01 00:00:00

abstract：:An aseptic vacuum extraction technique was used to obtain xylem fluid from the roots of rough lemon (Citrus jambhiri Lush.) rootstock of Florida citrus trees. Bacteria were consistently isolated from vascular fluid of both healthy and young tree decline-affected trees. Thirteen genera of bacteria were found, the most ...

journal_title：Applied and environmental microbiology

authors： Gardner JM,Feldman AW,Zablotowicz RM

更新日期：1982-06-01 00:00:00

abstract：:Thiosulfate-oxidizing sox gene homologues were found at four loci (I, II, III, and IV) on the genome of Bradyrhizobium japonicum USDA110, a symbiotic nitrogen-fixing bacterium in soil. In fact, B. japonicum USDA110 can oxidize thiosulfate and grow under a chemolithotrophic condition. The deletion mutation of the soxY(...

journal_title：Applied and environmental microbiology

authors： Masuda S,Eda S,Ikeda S,Mitsui H,Minamisawa K

更新日期：2010-04-01 00:00:00

abstract：:Intracellular pH (pH(in)) is a tightly regulated physiological parameter, which controls cell performance in all living systems. The purpose of this work was to evaluate if and how H(+) homeostasis is accomplished by an industrial wine strain of Saccharomyces cerevisiae while fermenting real must under the harsh winer...

journal_title：Applied and environmental microbiology

authors： Viana T,Loureiro-Dias MC,Loureiro V,Prista C

更新日期：2012-09-01 00:00:00

abstract：:Xenorhabdus nematophila engages in a mutualistic association with the nematode Steinernema carpocapsae. The nematode invades and traverses the gut of susceptible insects. X. nematophila is released in the insect blood (hemolymph), where it suppresses host immune responses and functions as a pathogen. X. nematophila pr...

journal_title：Applied and environmental microbiology

authors： Singh S,Reese JM,Casanova-Torres AM,Goodrich-Blair H,Forst S

更新日期：2014-07-01 00:00:00

abstract：:The size frequency distribution of planktonic cells of purple sulfur phototrophic bacteria was measured at several depths in a bacterial layer of Lake Cisó (Spain). The bacterioplankton was dominated by Chromatium minus (87 to 94% of the total biomass). The largest cells of C. minus were found in the top part of the b...

journal_title：Applied and environmental microbiology

authors： Montesinos E

更新日期：1987-04-01 00:00:00

abstract：:The kinetics of ethanol inhibition on cell growth and ethanol production by Kluyveromyces marxianus UCD (FST) 55-82 were studied during batch growth. The liquid medium contained 10% (wt/vol) inulin-type sugars derived from an extract of Jerusalem artichoke (Helianthus tuberosus) tubers, supplemented with small amounts...

journal_title：Applied and environmental microbiology

authors： Bajpai P,Margaritis A

更新日期：1982-12-01 00:00:00

abstract：:A bile salt hydrolase (BSH) was isolated from Bifidobacterium longum SBT2928, purified, and characterized. Furthermore, we describe for the first time cloning and analysis of the gene encoding BSH (bsh) in a member of the genus Bifidobacterium. The enzyme has a native molecular weight of 125,000 to 130,000 and a subun...

journal_title：Applied and environmental microbiology

authors： Tanaka H,Hashiba H,Kok J,Mierau I

更新日期：2000-06-01 00:00:00

abstract：:A lambda recombinant phage expressing beta-mannanase activity in Escherichia coli has been isolated from a genomic library of the extremely thermophilic anaerobe "Caldocellum saccharolyticum." The gene was cloned into pBR322 on a 5-kb BamHI fragment, and its location was obtained by deletion analysis. The sequence of ...

journal_title：Applied and environmental microbiology

authors： Lüthi E,Jasmat NB,Grayling RA,Love DR,Bergquist PL

更新日期：1991-03-01 00:00:00

abstract：:We have developed a microtiter plate method for screening a large number of bacterial isolates for the ability to grow on different crystalline polycyclic aromatic hydrocarbons (PAHs). Growth on PAHs cannot easily be determined with standard growth assays because of the very low aqueous solubility and bioavailability ...

journal_title：Applied and environmental microbiology

authors： Johnsen AR,Bendixen K,Karlson U

更新日期：2002-06-01 00:00:00

abstract：:In order to explore the biochemical scope of ochratoxin A-producing penicillia, we screened 48 Penicillium verrucosum isolates for the production of secondary metabolites. Fungal metabolites were analyzed by high-pressure liquid or gas chromatography coupled to diode array detection or mass spectrometry. The following...

journal_title：Applied and environmental microbiology

authors： Larsen TO,Svendsen A,Smedsgaard J

更新日期：2001-08-01 00:00:00

abstract：:A strain that efficiently degraded methyl tert-butyl ether (MTBE) was obtained by initial selection on the recalcitrant compound tert-butyl alcohol (TBA). This strain, a gram-positive methylotrophic bacterium identified as Mycobacterium austroafricanum IFP 2012, was also able to degrade tert-amyl methyl ether and tert...

journal_title：Applied and environmental microbiology

authors： François A,Mathis H,Godefroy D,Piveteau P,Fayolle F,Monot F

更新日期：2002-06-01 00:00:00

abstract：:During the summer of 1981, 3,887 sucrose-negative vibrios were isolated from seawater, sediment, plankton, and animal samples taken from 80 sites from Miami, Fla., to Portland, Maine. Of these, 4.2% were able to ferment lactose. The lactose-positive strains isolated from the various samples correlated positively with ...

journal_title：Applied and environmental microbiology

authors： Oliver JD,Warner RA,Cleland DR

更新日期：1983-03-01 00:00:00

abstract：:Marine infaunal burrows and tubes greatly enhance solute transport between sediments and the overlying water column and are sites of elevated microbial activity. Biotic and abiotic controls of the compositions and activities of burrow and tube microbial communities are poorly understood. The microbial communities in t...

journal_title：Applied and environmental microbiology

authors： Matsui GY,Ringelberg DB,Lovell CR

更新日期：2004-12-01 00:00:00

abstract：:Norovirus is the leading cause of food-related illness in the United States, and contamination of ready-to-eat items by food handlers poses a high risk for disease. This study reports the in vitro (suspension test) and in vivo (fingerpad protocol) assessments of a new ethanol-based hand sanitizer containing a synergis...

journal_title：Applied and environmental microbiology

authors： Macinga DR,Sattar SA,Jaykus LA,Arbogast JW

更新日期：2008-08-01 00:00:00

abstract：:Pigment production by the fungus Monascus sp. was studied to determine why Monascus sp. provides more pigment in solid culture than in submerged culture. Adding a sterilized nonionic polymeric adsorbent resin directly to the growing submerged culture did not enhance the pigment production, thus indicating that pigment...

journal_title：Applied and environmental microbiology

authors： Evans PJ,Wang HY

更新日期：1984-06-01 00:00:00

abstract：:Listeria monocytogenes is a food-borne, opportunistic, bacterial pathogen causing a wide spectrum of diseases, including meningitis, septicemia, abortion, and gastroenteritis, in humans and animals. Among the 13 L. monocytogenes serovars described, human listeriosis is mostly associated with strains of serovars 4b, 1/...

journal_title：Applied and environmental microbiology

authors： Severino P,Dussurget O,Vêncio RZ,Dumas E,Garrido P,Padilla G,Piveteau P,Lemaître JP,Kunst F,Glaser P,Buchrieser C

更新日期：2007-10-01 00:00:00

abstract：:Ethane oxidation was studied in ethane-grown resting cells (mycelia) of an Acremonium sp. and in cell-free preparations of such mycelia. From resting cell experiments evidence was found for a pathway of ethane oxidation via ethanol, acetaldehyde, and acetic acid. In vitro studies indicated that ethane-oxidizing activi...

journal_title：Applied and environmental microbiology

authors： Davies JS,Wellman AM,Zajic JE

更新日期：1976-07-01 00:00:00

abstract：:Seven different sources of inocula that included sediments, contaminated soils, groundwater, process effluent, and sludge were used to establish enrichment cultures of denitrifying bacteria on benzene, toluene, and xylenes in the absence of molecular oxygen. All of the enrichment cultures demonstrated complete depleti...

journal_title：Applied and environmental microbiology

authors： Evans PJ,Mang DT,Young LY

更新日期：1991-02-01 00:00:00

abstract：:The diversity of sulfate-reducing bacteria (SRB) in brackish sediment was investigated using small-subunit rRNA and dissimilatory sulfite reductase (DSR) gene clone libraries and cultivation. The phylogenetic affiliation of the most commonly retrieved clones for both genes was strikingly similar and produced Desulfosa...

journal_title：Applied and environmental microbiology

authors： Joulian C,Ramsing NB,Ingvorsen K

更新日期：2001-07-01 00:00:00

abstract：:Kinetic parameters and the role of cytochrome c(3) in sulfate, Fe(III), and U(VI) reduction were investigated in Desulfovibrio vulgaris Hildenborough. While sulfate reduction followed Michaelis-Menten kinetics (K(m) = 220 micro M), loss of Fe(III) and U(VI) was first-order at all concentrations tested. Initial reducti...

journal_title：Applied and environmental microbiology

authors： Elias DA,Suflita JM,McInerney MJ,Krumholz LR

更新日期：2004-01-01 00:00:00

abstract：:The use of the phospholipid fatty acid (PLFA) composition of microorganisms to detect previous self-heating events was studied in naturally self-heated peat and in peat incubated under temperature-controlled conditions. An increased content of total PLFAs was found in self-heated peat compared to that in unheated peat...

journal_title：Applied and environmental microbiology

authors： Ranneklev SB,Bååth E

更新日期：2003-06-01 00:00:00

abstract：:An organism tentatively identified as Ralstonia eutropha was isolated from enrichment cultures containing tetrahydrofurfuryl alcohol (THFA) as the sole source of carbon and energy. The strain was able to tolerate up to 200 mM THFA in mineral salt medium. The degradation was initiated by an inducible ferricyanide-depen...

journal_title：Applied and environmental microbiology

authors： Zarnt G,Schräder T,Andreesen JR

更新日期：1997-12-01 00:00:00

abstract：:The N-acetylglucosaminidase LytB of Streptococcus pneumoniae is involved in nasopharyngeal colonization and is responsible for cell separation at the end of cell division; thus, ΔlytB mutants form long chains of cells. This paper reports the construction and properties of a defective pneumococcal mutant producing an i...

journal_title：Applied and environmental microbiology

authors： Domenech M,García E

更新日期：2020-05-05 00:00:00

abstract：:Ground bark and heartwood from Alnus rubra and Pseudotsuga menziesii were added to a muddy sediment from a small Oregon stream and incubated in situ. Carbon dioxide and methane production rates were increased by all amendments, the biggest increase being shown with A. rubra wood. Except for sediment amended with A. ru...

journal_title：Applied and environmental microbiology

authors： Baker JH,Morita RY,Anderson NH

更新日期：1983-02-01 00:00:00

abstract：:Understanding the survival of fecal indicator bacteria (FIB) and microbial source-tracking (MST) markers is critical to developing pathogen fate and transport models. Although pathogen survival in water microcosms and manure-amended soils is well documented, little is known about their survival in intact cow pats depo...

journal_title：Applied and environmental microbiology

authors： Oladeinde A,Bohrmann T,Wong K,Purucker ST,Bradshaw K,Brown R,Snyder B,Molina M

更新日期：2014-01-01 00:00:00