Congruent phylogenies of most common small-subunit rRNA and dissimilatory sulfite reductase gene sequences retrieved from estuarine sediments.

Abstract:

:The diversity of sulfate-reducing bacteria (SRB) in brackish sediment was investigated using small-subunit rRNA and dissimilatory sulfite reductase (DSR) gene clone libraries and cultivation. The phylogenetic affiliation of the most commonly retrieved clones for both genes was strikingly similar and produced Desulfosarcina variabilis-like sequences from the inoculum but Desulfomicrobium baculatum-like sequences from a high dilution in natural media. Related organisms were subsequently cultivated from the site. PCR bias appear to be limited (or very similar) for the two primersets and target genes. However, the DSR primers showed a much higher phylogenetic specificity. DSR gene analysis is thus a promising and specific approach for investigating SRB diversity in complex habitats.

journal_name

Appl Environ Microbiol

authors

Joulian C,Ramsing NB,Ingvorsen K

doi

10.1128/AEM.67.7.3314-3318.2001

keywords:

subject

Has Abstract

pub_date

2001-07-01 00:00:00

pages

3314-8

issue

7

eissn

0099-2240

issn

1098-5336

journal_volume

67

pub_type

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