Abstract:
:Fermentation strategies for the production of poly(3-hydroxybutyrate) (PHB) from whey by recombinant Escherichia coli strain CGSC 4401 harboring the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes were developed. The pH-stat fed-batch cultures of E. coli CGSC 4401 harboring pJC4, a stable plasmid containing the A. latus PHA biosynthesis genes, were carried out with a concentrated whey solution containing 280 g of lactose equivalent per liter. Final cell and PHB concentrations of 119.5 and 96.2 g/liter, respectively, were obtained in 37.5 h, which resulted in PHB productivity of 2.57 g/liter/h.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Ahn WS,Park SJ,Lee SYdoi
10.1128/aem.66.8.3624-3627.2000keywords:
subject
Has Abstractpub_date
2000-08-01 00:00:00pages
3624-7issue
8eissn
0099-2240issn
1098-5336journal_volume
66pub_type
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