Microbial ecophysiology of whey biomethanation: characterization of bacterial trophic populations and prevalent species in continuous culture.

Abstract:

:The organization and species composition of bacterial trophic groups associated with lactose biomethanation were investigated in a whey-processing chemostat by enumeration, isolation, and general characterization studies. The bacteria were spatially organized as free-living forms and as self-immobilized forms appearing in flocs. Three dominant bacterial trophic group populations were present (in most probable number per milliliter) whose species numbers varied with the substrate consumed: hydrolytic, 10; acetogenic, 10 to 10; and methanogenic, 10 to 10. The three prevalent species utilizing lactose were identified as Leuconostoc mesenteroides, Klebsiella oxytoca, and Clostridium butyricum. Clostridium propionicum and Desulfovibrio vulgaris were the dominant lactate-consuming, hydrogen-producing acetogenic bacteria, while D. vulgaris was the only significant ethanol-degrading species. Methanosarcina barkeri and Methanothrix soehngenii were identified as the dominant acetate-utilizing methanogens, and Methanobacterium formicicum was the prevalent hydrogen-utilizing methanogen. A microbial food chain is proposed for lactose biomethanation that comprises multiple species in three different groups, with the major hydrogen-producing acetogen being a sulfate-reducing species, D. vulgaris, which functioned in the absence of significant levels of environmental sulfate.

journal_name

Appl Environ Microbiol

authors

Chartrain M,Zeikus JG

doi

10.1128/AEM.51.1.188-196.1986

keywords:

subject

Has Abstract

pub_date

1986-01-01 00:00:00

pages

188-96

issue

1

eissn

0099-2240

issn

1098-5336

journal_volume

51

pub_type

杂志文章
  • Lysis of a Lactococcus lactis Dipeptidase Mutant and Rescue by Mutation in the Pleiotropic Regulator CodY.

    abstract::Lactococcus lactis subsp. cremoris MG1363 is a model for the lactic acid bacteria (LAB) used in the dairy industry. The proteolytic system, consisting of a proteinase, several peptide and amino acid uptake systems, and a host of intracellular peptidases, plays a vital role in nitrogen metabolism and is of eminent impo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02937-19

    authors: Huang C,Hernandez-Valdes JA,Kuipers OP,Kok J

    更新日期:2020-04-01 00:00:00

  • Stress-Induced Evolution of Heat Resistance and Resuscitation Speed in Escherichia coli O157:H7 ATCC 43888.

    abstract::The development of resistance in foodborne pathogens to food preservation techniques is an issue of increasing concern, especially in minimally processed foods where safety relies on hurdle technology. In this context, mild heat can be used in combination with so-called nonthermal processes, such as high hydrostatic p...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02027-16

    authors: Gayán E,Cambré A,Michiels CW,Aertsen A

    更新日期:2016-10-27 00:00:00

  • Effects of growth pressure and temperature on Fatty Acid composition of a barotolerant deep-sea bacterium.

    abstract::The effects of pressure and temperature on the fatty acid composition in a barotolerant deep-sea bacterium that had branched-chain fatty acids were examined. The major fatty acids of the strain at atmospheric pressure were iso-C(15:0), C(16:1), iso-C(17:0), and iso-C(17:1). As the growth pressure increased, the propor...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.3.924-926.1993

    authors: Kamimura K,Fuse H,Takimura O,Yamaoka Y

    更新日期:1993-03-01 00:00:00

  • Spatial and temporal variation of phenanthrene-degrading bacteria in intertidal sediments.

    abstract::Phenanthrene-degrading bacteria were isolated from a 1-m2 intertidal sediment site in Boston Harbor. Samples were taken six times over 2 years. A total of 432 bacteria were isolated and characterized by biochemical testing. When clustered on the basis of phenotypic characteristics, the isolates could be separated into...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.7.2560-2565.1998

    authors: Berardesco G,Dyhrman S,Gallagher E,Shiaris MP

    更新日期:1998-07-01 00:00:00

  • Induction and transcription studies of the dextransucrase gene in Leuconostoc mesenteroides NRRL B-512F.

    abstract::Dextransucrase production by Leuconostoc mesenteroides NRRL B-512F in media containing carbon sources other than sucrose is reported for the first time. Dextransucrases were analyzed by gel electrophoresis and by an in situ activity assay. Their polymers and acceptor reaction products were also compared by (13)C nucle...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.12.5504-5509.1999

    authors: Quirasco M,López-Munguía A,Remaud-Simeon M,Monsan P,Farrés A

    更新日期:1999-12-01 00:00:00

  • Odorous Sulfur Compounds Emitted during Production of Compost Used as a Substrate in Mushroom Cultivation.

    abstract::Large-scale composting facilities are known to cause environmental problems, mainly through pungent air emitted by composting material. In air samples taken above stacks set up to prepare compost used as a substrate in mushroom cultivation, several volatile compounds were identified by means of the coupled techniques ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.1.176-180.1990

    authors: Derikx PJ,Op Den Camp HJ,van der Drift C,van Griensven LJ,Vogels GD

    更新日期:1990-01-01 00:00:00

  • Chemical disinfection of hepatitis A virus on environmental surfaces.

    abstract::Hepatitis A virus disinfection was assessed on contaminated stainless-steel disks. Ten microliters of fecally suspended hepatitis A virus was deposited on the center of each disk, dried for 20 min, and then covered with 20 microliters of the test product for 1 min. Of the 20 formulations tested, only 2% glutaraldehyde...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.11.3601-3604.1990

    authors: Mbithi JN,Springthorpe VS,Sattar SA

    更新日期:1990-11-01 00:00:00

  • Indirect Immunodetection of Fungal Fragments by Field Emission Scanning Electron Microscopy.

    abstract::Submicronic fungal fragments have been observed in in vitro aerosolization experiments. The occurrence of these particles has therefore been suggested to contribute to respiratory health problems observed in mold-contaminated indoor environments. However, the role of submicronic fragments in exacerbating adverse healt...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00929-15

    authors: Afanou KA,Straumfors A,Skogstad A,Nayak AP,Skaar I,Hjeljord L,Tronsmo A,Eduard W,Green BJ

    更新日期:2015-09-01 00:00:00

  • Artificial neural network prediction of viruses in shellfish.

    abstract::A database was probed with artificial neural network (ANN) and multivariate logistic regression (MLR) models to investigate the efficacy of predicting PCR-identified human adenovirus (ADV), Norwalk-like virus (NLV), and enterovirus (EV) presence or absence in shellfish harvested from diverse countries in Europe (Spain...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.9.5244-5253.2005

    authors: Brion G,Viswanathan C,Neelakantan TR,Lingireddy S,Girones R,Lees D,Allard A,Vantarakis A

    更新日期:2005-09-01 00:00:00

  • Stereochemistry of furfural reduction by a Saccharomyces cerevisiae aldehyde reductase that contributes to in situ furfural detoxification.

    abstract::Ari1p from Saccharomyces cerevisiae, recently identified as an intermediate-subclass short-chain dehydrogenase/reductase, contributes in situ to the detoxification of furfural. Furfural inhibits efficient ethanol production by yeast, particularly when the carbon source is acid-treated lignocellulose, which contains fu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00542-10

    authors: Bowman MJ,Jordan DB,Vermillion KE,Braker JD,Moon J,Liu ZL

    更新日期:2010-08-01 00:00:00

  • Expression of the isoamylase gene of Flavobacterium odoratum KU in Escherichia coli and identification of essential residues of the enzyme by site-directed mutagenesis.

    abstract::The isoamylase gene from Flavobacterium odoratum KU was cloned into and expressed in Escherichia coli JM109. The promoter of the gene was successful in E. coli, and the enzyme produced was excreted into the culture medium, depending on the amount of the enzyme expressed. The enzyme found in the culture medium showed a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.9.4163-4170.1999

    authors: Abe J,Ushijima C,Hizukuri S

    更新日期:1999-09-01 00:00:00

  • Genetic and transcriptional organization of the clpC locus in Bifidobacterium breve UCC 2003.

    abstract::A homolog of the clpC ATPase gene was identified in the genome of Bifidobacterium breve UCC 2003. Since this gene is very well conserved among eubacteria, we employed a PCR-based approach using primers based on highly conserved regions of ClpC proteins in order to identify homologous genes in other bifidobacterial spe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.10.6282-6291.2005

    authors: Ventura M,Fitzgerald GF,van Sinderen D

    更新日期:2005-10-01 00:00:00

  • pH Measurements of Agar Culture Media by Using a Flat-Bottom Electrode.

    abstract::A new design, flat-bottom combination pH electrode was evaluated for utility in determining the pH of prepared agar media by surface contact of the electrode with the agar media. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.34.2.242-243.1977

    authors: Gorski TW,Ritzert RW

    更新日期:1977-08-01 00:00:00

  • Chemical and Physical Characterization of Interfacial-Active Lipids from Rhodococcus erythropolis Grown on n-Alkanes.

    abstract::Lipophilic compounds of the culture suspension containing Rhodococcus erythropolis DSM43215 had surfactant properties when the bacteria were cultivated with n-alkanes as the sole carbon source. Thirteen main components from a dichloromethane-methanol extract of the R. erythropolis cultures were isolated and characteri...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.44.4.864-870.1982

    authors: Kretschmer A,Bock H,Wagner F

    更新日期:1982-10-01 00:00:00

  • Molecular cloning of cyanobacterial pteridine glycosyltransferases that catalyze the transfer of either glucose or xylose to tetrahydrobiopterin.

    abstract::Here, we report cloning of cyanobacterial genes encoding pteridine glycosyltransferases that catalyze glucosyl or xylosyl transfer from UDP-sugars to tetrahydrobiopterin. The genes were cloned by PCR amplification from genomic DNA which was isolated from culture and environmental samples and overexpressed in Escherich...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01083-10

    authors: Lee YG,Kim AH,Park MB,Kim HL,Lee KH,Park YS

    更新日期:2010-11-01 00:00:00

  • Molybdenum Availability Is Key to Nitrate Removal in Contaminated Groundwater Environments.

    abstract::The concentrations of molybdenum (Mo) and 25 other metals were measured in groundwater samples from 80 wells on the Oak Ridge Reservation (ORR) (Oak Ridge, TN), many of which are contaminated with nitrate, as well as uranium and various other metals. The concentrations of nitrate and uranium were in the ranges of 0.1 ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00917-15

    authors: Thorgersen MP,Lancaster WA,Vaccaro BJ,Poole FL,Rocha AM,Mehlhorn T,Pettenato A,Ray J,Waters RJ,Melnyk RA,Chakraborty R,Hazen TC,Deutschbauer AM,Arkin AP,Adams MW

    更新日期:2015-08-01 00:00:00

  • Improved microbiological assay procedures for dihydrostreptomycin residues in milk and dairy products.

    abstract::Because dihydrostreptomycin can remain as a slowly removed antibiotic residue in dairy animals and because of the need for more sensitive procedures with which to provide information concerning antibiotic residues in food products, procedures were developed in more sensitive assays of dihydrostreptomycin in milk and s...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.35.3.517-520.1978

    authors: Inglis JM,Katz SE

    更新日期:1978-03-01 00:00:00

  • Electron donor cytochrome b5 is required for hyphal tip accumulation of sterol-rich plasma membrane domains and membrane fluidity in Aspergillus fumigatus.

    abstract::The electron donor cytochrome b5 (CybE/Cyb5) fuels the activity of the ergosterol biosynthesis-related P450 enzymes/P450s by providing electrons to P450s to promote ergosterol biosynthesis. Previous studies reported that lack of Aspergillus fumigatus (A. fumigatus) CybE reduces the proportion of ergosterol in total st...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02571-20

    authors: Zhang C,Ren Y,Gao L,Gu H,Lu L

    更新日期:2020-11-30 00:00:00

  • Risk of handling as a route of exposure to infectious waterborne Cryptosporidium parvum oocysts via Atlantic blue crabs (Callinectes sapidus).

    abstract::Commercial Atlantic blue crabs (Callinectes sapidus) were exposed to 2.0x10(4) infectious waterborne oocysts of Cryptosporidium parvum. The study demonstrated that blue crabs can transfer C. parvum oocysts to persons involved in handling or preparing crabs and that they may contaminate other surfaces or products durin...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00166-07

    authors: Graczyk TK,McOliver C,Silbergeld EK,Tamang L,Roberts JD

    更新日期:2007-06-01 00:00:00

  • Sucker-like structures on the pathogenic amoeba Naegleria fowleri.

    abstract::Using scanning electron microscopy, we observed sucker-like structures on amoebae of 13 human isolates of Naegleria fowleri. The number of suckers per amoeba seemed to vary according to the virulence of the strain. We propose the term amoebastome to describe this unique sucker-like structure of N. fowleri. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.47.1.12-14.1984

    authors: John DT,Cole TB Jr,Marciano-Cabral FM

    更新日期:1984-01-01 00:00:00

  • The cyclic AMP (cAMP)-cAMP receptor protein signaling system mediates resistance of Vibrio cholerae O1 strains to multiple environmental bacteriophages.

    abstract::Toxigenic Vibrio cholerae, the causative agent of the epidemic diarrheal disease cholera, interacts with diverse environmental bacteriophages. These interactions promote genetic diversity or cause selective enrichment of phage-resistant bacterial clones. To identify bacterial genes involved in mediating the phage-resi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00008-10

    authors: Zahid MS,Waise TM,Kamruzzaman M,Ghosh AN,Nair GB,Mekalanos JJ,Faruque SM

    更新日期:2010-07-01 00:00:00

  • Temperature affects species distribution in symbiotic populations of Vibrio spp.

    abstract::The genus Sepiola (Cephalopoda: Sepiolidae) contains 10 known species that occur in the Mediterranean Sea today. All Sepiola species have a light organ that contains at least one of two species of luminous bacteria, Vibrio fischeri and Vibrio logei. The two Vibrio species coexist in at least four Sepiola species (S. a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.8.3550-3555.2000

    authors: Nishiguchi MK

    更新日期:2000-08-01 00:00:00

  • Microcystin production by Microcystis aeruginosa in a phosphorus-limited chemostat.

    abstract::The production of microcystins (MC) from Microcystis aeruginosa UTEX 2388 was investigated in a P-limited continuous culture. MC (MC-LR, MC-RR, and MC-YR) from lyophilized M. aeruginosa were extracted with 5% acetic acid, purified by a Sep-Pak C(18) cartridge, and then analyzed by high-performance liquid chromatograph...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.1.176-179.2000

    authors: Oh HM,Lee SJ,Jang MH,Yoon BD

    更新日期:2000-01-01 00:00:00

  • Secondary metabolites help biocontrol strain Pseudomonas fluorescens CHA0 to escape protozoan grazing.

    abstract::In soil ecosystems, bacteria must cope with predation activity, which is attributed mainly to protists. The development of antipredation strategies may help bacteria maintain higher populations and persist longer in the soil. We analyzed the interaction between the root-colonizing and biocontrol strain Pseudomonas flu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00557-06

    authors: Jousset A,Lara E,Wall LG,Valverde C

    更新日期:2006-11-01 00:00:00

  • Synergistic degradation of linuron by a bacterial consortium and isolation of a single linuron-degrading variovorax strain.

    abstract::The bacterial community composition of a linuron-degrading enrichment culture and the role of the individual strains in linuron degradation have been determined by a combination of methods, such as denaturing gradient gel electrophoresis of the total 16S rRNA gene pool, isolation and identification of strains, and bio...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.3.1532-1541.2003

    authors: Dejonghe W,Berteloot E,Goris J,Boon N,Crul K,Maertens S,Höfte M,De Vos P,Verstraete W,Top EM

    更新日期:2003-03-01 00:00:00

  • Novel surface display system for proteins on non-genetically modified gram-positive bacteria.

    abstract::A novel display system is described that allows highly efficient immobilization of heterologous proteins on bacterial surfaces in applications for which the use of genetically modified bacteria is less desirable. This system is based on nonliving and non-genetically modified gram-positive bacterial cells, designated g...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.72.1.880-889.2006

    authors: Bosma T,Kanninga R,Neef J,Audouy SA,van Roosmalen ML,Steen A,Buist G,Kok J,Kuipers OP,Robillard G,Leenhouts K

    更新日期:2006-01-01 00:00:00

  • The motility symbiont of the termite gut flagellate Caduceia versatilis is a member of the "Synergistes" group.

    abstract::The flagellate Caduceia versatilis in the gut of the termite Cryptotermes cavifrons reportedly propels itself not by its own flagella but solely by the flagella of ectosymbiotic bacteria. Previous microscopic observations have revealed that the motility symbionts are flagellated rods partially embedded in the host cel...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00750-07

    authors: Hongoh Y,Sato T,Dolan MF,Noda S,Ui S,Kudo T,Ohkuma M

    更新日期:2007-10-01 00:00:00

  • Sequence heterogeneity in the two 16S rRNA genes of Phormium yellow leaf phytoplasma.

    abstract::Phormium yellow leaf (PYL) phytoplasma causes a lethal disease of the monocotyledon, New Zealand flax (Phormium tenax). The 16S rRNA genes of PYL phytoplasma were amplified from infected flax by PCR and cloned, and the nucleotide sequences were determined. DNA sequencing and Southern hybridization analysis of genomic ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.9.3133-3139.1996

    authors: Liefting LW,Andersen MT,Beever RE,Gardner RC,Forster RL

    更新日期:1996-09-01 00:00:00

  • Characterization of geographically distinct bacterial communities associated with coral mucus produced by Acropora spp. and Porites spp.

    abstract::Acropora and Porites corals are important reef builders in the Indo-Pacific and Caribbean. Bacteria associated with mucus produced by Porites spp. and Acropora spp. from Caribbean (Punta Maroma, Mexico) and Indo-Pacific (Hoga and Sampela, Indonesia) reefs were determined. Analysis of pyrosequencing libraries showed th...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.07764-11

    authors: McKew BA,Dumbrell AJ,Daud SD,Hepburn L,Thorpe E,Mogensen L,Whitby C

    更新日期:2012-08-01 00:00:00

  • Lactic Acid Bacteria in Durum Wheat Flour Are Endophytic Components of the Plant during Its Entire Life Cycle.

    abstract::This study aimed at assessing the dynamics of lactic acid bacteria and other Firmicutes associated with durum wheat organs and processed products. 16S rRNA gene-based high-throughput sequencing showed that Lactobacillus, Streptococcus, Enterococcus, and Lactococcus were the main epiphytic and endophytic genera among l...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01852-15

    authors: Minervini F,Celano G,Lattanzi A,Tedone L,De Mastro G,Gobbetti M,De Angelis M

    更新日期:2015-10-01 00:00:00