Artificial neural network prediction of viruses in shellfish.

abstract：:A method capable of detecting as little as 0.11 U of xylanase activity in polyacrylamide gels was developed. The method entails incubation of protein gels in contact with substrate gels containing unmodified xylan, followed by immersion of substrate gels in 95% ethanol. Resulting zymograms contain transparent bands co...

journal_title：Applied and environmental microbiology

authors： Royer JC,Nakas JP

更新日期：1990-06-01 00:00:00

abstract：:Vibrio cholerae can shift to a "rugose" phenotype, thereby producing copious exopolysaccharide (EPS), which promotes its environmental survival and persistence. We report conditions that promote high-frequency rugose EPS production (HFRP), whereby cells switch at high frequency (up to 80%) to rugose EPS production. HF...

journal_title：Applied and environmental microbiology

authors： Ali A,Rashid MH,Karaolis DK

更新日期：2002-11-01 00:00:00

abstract：:Marker rescue is an important molecular technique that enables sequential gene deletions. The Cre-loxP recombination system has been used for marker gene rescue in various organisms, including aspergilli. However, this system requires many time-consuming steps, including construction of a Cre expression plasmid, intro...

journal_title：Applied and environmental microbiology

authors： Mizutani O,Masaki K,Gomi K,Iefuji H

更新日期：2012-06-01 00:00:00

abstract：:The presence of enteroviruses in oysters and oyster-harvesting waters of the Texas Gulf coast was monitored over a period of 10 months. Viruses were detected in water and oyster samples obtained from areas both open and closed to shellfish harvesting. Viruses were detected periodically in waters that met current bacte...

journal_title：Applied and environmental microbiology

authors： Goyal SM,Gerba CP,Melnick JL

更新日期：1979-03-01 00:00:00

abstract：:A sensitive and specific method for detection of Listeria monocytogenes in milk and ground-beef samples is described. It consists of culturing samples in listeria enrichment broth (LEB) and subculturing them from LEB to listeria plating media, followed by DNA extraction and species-specific detection of the organism b...

journal_title：Applied and environmental microbiology

authors： Thomas EJ,King RK,Burchak J,Gannon VP

更新日期：1991-09-01 00:00:00

abstract：:The bacterial host ranges of the conjugatively self-transmissible IncN group plasmid pCU1 and its mobilizable miniderivative, pCU785, were examined. Species of the gram-negative purple bacteria were chosen for this study. Conjugative mobilization of pCU785 into a wide variety of bacteria was facilitated by the presenc...

journal_title：Applied and environmental microbiology

authors： Krishnan BR,Iyer VN

更新日期：1988-09-01 00:00:00

abstract：:Biofilms were grown from wild-type (WT) Pseudomonas aeruginosa PAO1 and the cell signaling lasI mutant PAO1-JP1 under laminar and turbulent flows to investigate the relative contributions of hydrodynamics and cell signaling for biofilm formation. Various biofilm morphological parameters were quantified using Image Str...

journal_title：Applied and environmental microbiology

authors： Purevdorj B,Costerton JW,Stoodley P

更新日期：2002-09-01 00:00:00

abstract：:Twenty-six lactic acid bacterium strains isolated from European dairy products were identified as Streptococcus thermophilus and characterized by bacterial growth and exopolysaccharide (EPS)-producing capacity in milk and enriched milk medium. In addition, the acidification rates of the different strains were compared...

journal_title：Applied and environmental microbiology

authors： Vaningelgem F,Zamfir M,Mozzi F,Adriany T,Vancanneyt M,Swings J,De Vuyst L

更新日期：2004-02-01 00:00:00

abstract：:A European baseline survey during the years 2005 and 2006 has revealed that the monophasic Salmonella enterica subsp. enterica serovar 4,12:d:- was, with a prevalence of 23.6%, the most frequently isolated serovar in German broiler flocks. In Denmark and the United Kingdom, its serovar prevalences were 15.15% and 2.8%...

journal_title：Applied and environmental microbiology

authors： Huehn S,Bunge C,Junker E,Helmuth R,Malorny B

更新日期：2009-02-01 00:00:00

abstract：:Streptomyces subtilisin inhibitor-like proteins were found to be distributed widely in streptomycetes by using the combination of the convenient, newly developed plate assay system and an established liquid culture assay. Almost all the strains formerly categorized as Streptoverticillium species produced proteins that...

journal_title：Applied and environmental microbiology

authors： Taguchi S,Kikuchi H,Suzuki M,Kojima S,Terabe M,Miura K,Nakase T,Momose H

更新日期：1993-12-01 00:00:00

abstract：:Two-dimensional polyacrylamide electrophoresis was used to determine that mutants of Rhizobium trifolii DT6, claimed to be capable of effectively nodulating soybeans, were actually Rhizobium japonicum 110 contaminants isolated from the parent DT6 culture. ...

journal_title：Applied and environmental microbiology

authors： Leps WT,Roberts GP,Brill WJ

更新日期：1980-02-01 00:00:00

abstract：:We investigated the effects of bottle enclosure on autotrophic and heterotrophic picoplankton in North and South subtropical Atlantic oligotrophic waters, where the biomass and metabolism of the microbial community are dominated by the picoplankton size class. We measured changes in both autotrophic (Prochlorococcus, ...

journal_title：Applied and environmental microbiology

authors： Calvo-Díaz A,Díaz-Pérez L,Suárez LÁ,Morán XA,Teira E,Marañón E

更新日期：2011-08-15 00:00:00

abstract：:A total of 52 strains of Lactobacillus acidophilus were examined for production of bacteriocins. A majority (63%) demonstrated inhibitory activity against all members of a four-species grouping of Lactobacillus leichmannii, Lactobacillus bulgaricus, Lactobacillus helveticus, and Lactobacillus lactis. Four L. acidophil...

journal_title：Applied and environmental microbiology

authors： Barefoot SF,Klaenhammer TR

更新日期：1983-06-01 00:00:00

abstract：:To enhance the production of isoprene, a volatile 5-carbon hydrocarbon, in the Gram-positive spore-forming rod-shaped bacterium Bacillus subtilis, 1-deoxy-d-xylulose-5-phosphate synthase (Dxs) and 1-deoxy-d-xylulose-5-phosphate reductoisomerase (Dxr) were overexpressed in B. subtilis DSM 10. For the strain that overex...

journal_title：Applied and environmental microbiology

authors： Xue J,Ahring BK

更新日期：2011-04-01 00:00:00

abstract：:Correlations between the biomass of phytoplankton and the biomass of bacteria and between the biomass of bacteria and the biomass of protozoans suggest that there is coupling between these compartments of the "microbial loop." To investigate this coupling on the species level, bacteria and protozoans from untreated la...

journal_title：Applied and environmental microbiology

authors： van Hannen EJ,Mooij W,van Agterveld MP,Gons HJ,Laanbroek HJ

更新日期：1999-06-01 00:00:00

abstract：:The transport characteristics of two adhesion-deficient, indigenous groundwater strains, Comamonas sp. strain DA001 and Erwinia herbicola OYS2-A, were studied by using intact sediment cores (7 by 50 cm) from Oyster, Va. Both strains are gram-negative rods (1.10 by 0.56 and 1.56 by 0.46 microm, respectively) with stron...

journal_title：Applied and environmental microbiology

authors： Dong H,Rothmel R,Onstott TC,Fuller ME,DeFlaun MF,Streger SH,Dunlap R,Fletcher M

更新日期：2002-05-01 00:00:00

abstract：:Staphylococcus aureus is a gram-positive pathogen that causes a variety of diseases, including bovine mastitis, which has severe economic consequences. Standard antibiotic treatment results in selection of resistant strains, leading to a need for alternative treatments, such as bacteriophage therapy. Forty-nine S. aur...

journal_title：Applied and environmental microbiology

authors： Synnott AJ,Kuang Y,Kurimoto M,Yamamichi K,Iwano H,Tanji Y

更新日期：2009-07-01 00:00:00

abstract：:Relative to those of unexposed cultures, the transcript levels of the four CprA-type reductive dehalogenase genes (cprA2, cprA3, cprA4, and cprA5) in Desulfitobacterium hafniense PCP-1 were measured in cultures exposed to chlorophenols. In 2,4,6-trichlorophenol-amended cultures, cprA2 and cprA3 were upregulated, as wa...

journal_title：Applied and environmental microbiology

authors： Bisaillon A,Beaudet R,Lépine F,Villemur R

更新日期：2011-09-01 00:00:00

abstract：:Wild animals living close to cattle and pig farms (four each) were examined for verocytotoxin-producing Escherichia coli (VTEC; also known as Shiga toxin-producing E. coli). The prevalence of VTEC among the 260 samples from wild animals was generally low. However, VTEC isolates from a starling (Sturnus vulgaris) and a...

journal_title：Applied and environmental microbiology

authors： Nielsen EM,Skov MN,Madsen JJ,Lodal J,Jespersen JB,Baggesen DL

更新日期：2004-11-01 00:00:00

abstract：:The effects of silicic acid on the growth of Thermus thermophilus TMY, an extreme thermophile isolated from a siliceous deposit formed from geothermal water at a geothermal power plant in Japan, were examined at 75 degrees C. At concentrations higher than the solubility of amorphous silica (400 to 700 ppm SiO(2)), a s...

journal_title：Applied and environmental microbiology

authors： Doi K,Fujino Y,Inagaki F,Kawatsu R,Tahara M,Ohshima T,Okaue Y,Yokoyama T,Iwai S,Ogata S

更新日期：2009-04-01 00:00:00

abstract：:The luminescence signal of luxAB-encoded bacterial luciferase strongly depends on the metabolic state of the host cell, which restricts the use of this reporter system to metabolically active bacteria. Here we show that in stationary-phase cells of Lactococcus lactis, detection of luciferase is significantly improved ...

journal_title：Applied and environmental microbiology

authors： Bachmann H,Santos F,Kleerebezem M,van Hylckama Vlieg JE

更新日期：2007-07-01 00:00:00

abstract：:The effects of environmental stress exposure on Listeria monocytogenes growth and virulence-associated characteristics were investigated. Specifically, we measured the effects of temperature (7 or 37 degrees C), pH (5.5 or 7.4), the presence of salt and organic acids (375 mM NaCl, 8.45 mM sodium diacetate [SD], 275 mM...

journal_title：Applied and environmental microbiology

authors： Garner MR,James KE,Callahan MC,Wiedmann M,Boor KJ

更新日期：2006-08-01 00:00:00

abstract：:A bacterial strain (strain S5) which grows aerobically with the sulfonated azo compound 4-carboxy-4'-sulfoazobenzene as the sole source of carbon and energy was isolated. This strain was obtained by continuous adaptation of "Hydrogenophaga palleronii" S1, which has the ability to grow aerobically with 4-aminobenzenesu...

journal_title：Applied and environmental microbiology

authors： Blümel S,Contzen M,Lutz M,Stolz A,Knackmuss HJ

更新日期：1998-06-01 00:00:00

abstract：:Genes of Salmonella enterica serovar Typhimurium LT2 expected to be specifically present in Salmonella were selected using the Basic Local Alignment Search Tool (BLAST) program. The 152 selected genes were compared with 11 genomic sequences of Salmonella serovars, including Salmonella enterica subsp. I and IIIb and Sa...

journal_title：Applied and environmental microbiology

authors： Kim HJ,Park SH,Kim HY

更新日期：2006-09-01 00:00:00

abstract：:Current approaches used for dose-response modeling of low-dose exposures of pathogens rely on assumptions and extrapolations. These models are important for quantitative microbial risk assessment of food. A mechanistic framework has been advocated as an alternative approach for evaluating dose-response relationships. ...

journal_title：Applied and environmental microbiology

authors： Abe H,Koyama K,Koseki S

更新日期：2020-12-17 00:00:00

abstract：:A Rhizobium strain isolated from stem nodules of the legume Sesbania rostrata was shown to grow on atmospheric nitrogen (N(2)) as the sole nitrogen source. Non-N(2)-fixing mutants isolated directly on agar plates formed nodules that did not fix N(2) when inoculated into the host plant. ...

journal_title：Applied and environmental microbiology

authors： Dreyfus BL,Elmerich C,Dommergues YR

更新日期：1983-02-01 00:00:00

abstract：:By using gas chromatography-mass spectrometry, seven volatile compounds were identified in vacuum-packaged sliced corned beef spoiled by Brochothrix thermosphacta under aerobic conditions. Acetoin and diacetyl appeared to be of major sensory significance. ...

journal_title：Applied and environmental microbiology

authors： Stanley G,Shaw KJ,Egan AF

更新日期：1981-03-01 00:00:00

abstract：:Acetaldehyde is a toxic compound produced by Saccharomyces cerevisiae cells under several growth conditions. The adverse effects of this molecule are important, as significant amounts accumulate inside the cells. By means of global gene expression analyses, we have detected the effects of acetaldehyde addition in the ...

journal_title：Applied and environmental microbiology

authors： Aranda A,del Olmo ML

更新日期：2004-04-01 00:00:00

abstract：:Thermal inactivation of desiccation-adapted Salmonella spp. in aged chicken litter was investigated in comparison with that in a nonadapted control to examine potential cross-tolerance of desiccation-adapted cells to heat treatment. A mixture of four Salmonella serovars was inoculated into the finished compost with 20...

journal_title：Applied and environmental microbiology

authors： Chen Z,Diao J,Dharmasena M,Ionita C,Jiang X,Rieck J

更新日期：2013-11-01 00:00:00

abstract：:The arginine deiminase system was found to function in protecting bacterial cells against the damaging effects of acid environments. For example, as little as 2.9 mM arginine added to acidified suspensions of Streptococcus sanguis at a pH of 4.0 resulted in ammonia production and protection against killing. The argini...

journal_title：Applied and environmental microbiology

authors： Casiano-Colón A,Marquis RE

更新日期：1988-06-01 00:00:00