Abstract:
:A Rhizobium strain isolated from stem nodules of the legume Sesbania rostrata was shown to grow on atmospheric nitrogen (N(2)) as the sole nitrogen source. Non-N(2)-fixing mutants isolated directly on agar plates formed nodules that did not fix N(2) when inoculated into the host plant.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Dreyfus BL,Elmerich C,Dommergues YRdoi
10.1128/AEM.45.2.711-713.1983keywords:
subject
Has Abstractpub_date
1983-02-01 00:00:00pages
711-3issue
2eissn
0099-2240issn
1098-5336journal_volume
45pub_type
杂志文章abstract::A model conditional-suicide system to control genetically engineered microorganisms able to degrade substituted benzoates is reported. The system is based on two elements. One element consists of a fusion between the promoter of the Pseudomonas putida TOL plasmid-encoded meta-cleavage pathway operon (P(m)) and the lac...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.57.5.1504-1508.1991
更新日期:1991-05-01 00:00:00
abstract::The adherent-invasive Escherichia coli (AIEC) pathotype, which has been associated with Crohn's disease, shows similar traits to human and animal extraintestinal pathogenic E. coli (ExPEC) with respect to their phylogenetic origin and virulence gene profiles. Here, we demonstrate that animal ExPEC strains generally do...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02614-10
更新日期:2011-08-15 00:00:00
abstract::The structural gene coding for the lysine-sensitive aspartokinase II of the methylotrophic thermotolerant Bacillus sp. strain MGA3 was cloned from a genomic library by complementation of an Escherichia coli auxotrophic mutant lacking all three aspartokinase isozymes. The nucleotide sequence of the entire 2.2-kb PstI f...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.9.2806-2814.1992
更新日期:1992-09-01 00:00:00
abstract::To enhance food safety and stability, the food industry tends to use natural antimicrobials such as plant-derived compounds as an attractive alternative to chemical preservatives. Nonetheless, caution must be exercised in light of the potential for bacterial adaptation to these molecules, a phenomenon previously obser...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01480-10
更新日期:2011-02-01 00:00:00
abstract::Oxolinic acid (OA), a quinolone, inhibits the activity of DNA gyrase composed of GyrA and GyrB and shows antibacterial activity against Burkholderia glumae. Since B. glumae causes bacterial seedling rot and grain rot of rice, both of which are devastating diseases, the emergence of OA-resistant bacteria has important ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.70.9.5613-5620.2004
更新日期:2004-09-01 00:00:00
abstract::The cryptic plasmid pDM6 was isolated from late exponential-phase cells of Clostridium acetobutylicum NCIB 6444 by either alkaline lysis or electroporation. The application of high voltage during electroporation resulted in higher DNA yield than did the alkaline lysis procedure. However, electroporation-induced plasmi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.6.1725-1728.1990
更新日期:1990-06-01 00:00:00
abstract::3-Hydroxypyridine (3HP) is an important natural pyridine derivative. Ensifer adhaerens HP1 can utilize 3HP as its sole sources of carbon, nitrogen, and energy to grow, but the genes responsible for the degradation of 3HP remain unknown. In this study, we predicted that a gene cluster, designated 3hpd, might be respons...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01313-20
更新日期:2020-09-17 00:00:00
abstract::A stable shuttle vector which replicates in Escherichia coli and Clostridium perfringens was constructed by ligating a 3.6-kilobase (kb) fragment of plasmid pBR322 with C. perfringens plasmid pHB101 (3.1 kb). The marker for this shuttle plasmid originated from the 1.3-kb chloramphenicol resistance gene of plasmid pHR1...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.55.2.360-365.1989
更新日期:1989-02-01 00:00:00
abstract::Pseudomonas aeruginosa is an opportunistic human pathogen and has been established as a model organism to study bacterial biofilm formation. At least three exopolysaccharides (alginate, Psl, and Pel) contribute to the formation of biofilms in this organism. Here mutants deficient in the production of one or more of th...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00637-11
更新日期:2011-08-01 00:00:00
abstract::Pyrene is a regulated pollutant at sites contaminated with polycyclic aromatic hydrocarbons (PAH). It is mineralized by some bacteria but is also transformed to nonmineral products by a variety of other PAH-degrading bacteria. We examined the formation of such products by four bacterial strains and identified and furt...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.5.1917-1922.2000
更新日期:2000-05-01 00:00:00
abstract::Thirty-two chemoheterotrophic bacteria were isolated from unsaturated subsurface soil samples obtained from ca. 70 m below land surface in a high desert in southeastern Idaho. Most isolates were gram positive (84%) and strict aerobes (79%). Acridine orange direct counts of microbes in one subsurface sample showed lowe...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.55.9.2420-2423.1989
更新日期:1989-09-01 00:00:00
abstract::The food-borne pathogen Vibrio parahaemolyticus has been reported as being present in New Zealand (NZ) seawaters, but there have been no reported outbreaks of food-borne infection from commercially grown NZ seafood. Our study determined the current incidence of V. parahaemolyticus in NZ oysters and Greenshell mussels ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.04020-14
更新日期:2015-04-01 00:00:00
abstract::Addition of 2 mg of Pb2+/g of soil concident with or after amendment with starch or maltose resulted in 75 and 50% decreases in net synthesis of amylase and alpha-glucosidase, respectively. Invertase synthesis in sucrose-amended soil was transiently reduced after Pb2+ addition. Amylase activity was several times less ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.33.2.262-268.1977
更新日期:1977-02-01 00:00:00
abstract::The cytopathology for rat neuroblastoma cells (B-103) and the pathogenicity for B6C3F1 mice of four species of Naegleria have been compared. Both live amoebae and cell-free extracts of N. australiensis, N. fowleri, N. gruberi, and N. lovaniensis added to 51Cr-labeled B-103 cells caused release of radiolabel. All four ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.51.5.1133-1137.1986
更新日期:1986-05-01 00:00:00
abstract::A thorough understanding of the microorganisms and pathogens associated with the larval stage of the tropical ornate rock lobster, Panulirus ornatus, is required to overcome disease outbreaks that currently block aquaculture attempts. This study used microscopy in addition to culture and molecularly based microbiologi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02520-06
更新日期:2007-03-01 00:00:00
abstract::Malonyl-coenzyme A (CoA) decarboxylase, malonyl-CoA synthetase, and malonate transporter mutants of Rhizobium leguminosarum bv. viciae and trifolii fixed N2 at wild-type rates on pea and clover, respectively. Thus, malonate does not drive N2 fixation in legume nodules. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00919-13
更新日期:2013-07-01 00:00:00
abstract::Due to the complex microecology and microenvironment of dental plaque, novel caries prevention strategies require modulating the microbial communities ecologically and reducing the cariogenic properties effectively. Antimicrobial peptide GH12 reduced the lactic acid production and exopolysaccharide (EPS) synthesis of ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00527-20
更新日期:2020-07-02 00:00:00
abstract::This study concerns the exploitation of the lytic enzyme enterolysin A (EntL), produced by Enterococcus faecalis strain DPC5280, to elicit the controlled autolysis of starter lactococci. EntL, a cell wall metalloendopeptidase secreted by some E. faecalis strains, can kill a wide range of gram-positive bacteria, includ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.70.3.1744-1748.2004
更新日期:2004-03-01 00:00:00
abstract::During the course of immunologic studies involving the gastrointestinal colonization of mice with Candida albicans, it became apparent that the animals were being exposed to large numbers of Aspergillus fumigatus spores which interfered with the C. albicans colonization. To determine the source of the A. fumigatus exp...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.6.2297-2301.1995
更新日期:1995-06-01 00:00:00
abstract::A novel halophilic and metal-reducing bacterium, Orenia metallireducens strain Z6, was isolated from briny groundwater extracted from a 2.02 km-deep borehole in the Illinois Basin, IL. This organism shared 96% 16S rRNA gene similarity with Orenia marismortui but demonstrated physiological properties previously unknown...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02382-16
更新日期:2016-10-14 00:00:00
abstract::A novel multiplex PCR method using three sets of specific primers was developed for the detection of the cytotoxic (act), heat-labile (alt), and heat-stable (ast) enterotoxin genes in Aeromonas spp. This assay was used to characterize 35 reference strains as well as 537 food-borne isolates. A total of seven gene patte...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01357-09
更新日期:2010-01-01 00:00:00
abstract::This study was aimed at assessing whether the repeated exposure of 12 strains of Salmonella spp., Escherichia coli, and Listeria monocytogenes to alternative nonthermal decontamination techniques with UV light (UV-C) and nonthermal atmospheric plasma (NTAP) may cause the emergence of variants showing increased resista...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00102-20
更新日期:2020-04-17 00:00:00
abstract::Phragmidium violaceum causes leaf rust on the European blackberry (Rubus fruticosus L. aggregate). Multiple strains of this pathogen have been introduced into southern Australia for the biological control of at least 15 taxa of European blackberry, a nonindigenous, invasive plant. In climates conducive to leaf rust, t...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02885-07
更新日期:2008-09-01 00:00:00
abstract::Research on the microbial communities that colonize stone monuments may provide a new understanding of stone biodeterioration and microbe-induced carbonate precipitation. This work investigated the seasonal variation of microbial communities in 2016 and 2017, as well as its effects on stone monuments. We determined th...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02680-17
更新日期:2018-03-19 00:00:00
abstract::Nontoxigenic strains of Clostridium botulinum types C and D are converted to toxigenic strains by infection with specific Tox+ bacteriophages. The nucleic acids were extracted from five converting phages, c-st, c-468, c-203, c-d6f, and d-1873, and one nonconverting phage, c-n71, and treated with nucleases. The nucleic...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.1.69-73.1988
更新日期:1988-01-01 00:00:00
abstract::Francisella tularensis is found in a wide variety of hosts and extrahost environments, making culture recovery a diagnostic challenge. Here we demonstrate improved recovery times and good sensitivity (90%) when cultures were inoculated on the site of an investigation using fresh tissues. For contaminated specimens, an...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.70.6.3733-3735.2004
更新日期:2004-06-01 00:00:00
abstract::Vibrio cholerae O1 strain TSI-4 (El Tor, Ogawa) can shift to a rugose colony morphology from its normal translucent colony morphology in response to nutrient starvation. We have investigated differences between the rugose and translucent forms of V. cholerae O1 strain TSI-4. Electron microscopic examination of the rug...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.10.3648-3655.1998
更新日期:1998-10-01 00:00:00
abstract::Graded doses of pure ochratoxin A (0, 0.5, 1.0, 2.0, 4.0, and 8.0 microgram of toxin per g of feed) were incorporated into a commercial diet which was fed to chickens from hatching to 3 weeks of age, at which time the experiments were terminated. Liver glycogen levels were elevated significantly (P less than 0.05) by ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.37.1.122-126.1979
更新日期:1979-01-01 00:00:00
abstract::Aspergillus niger is known for its efficient excretion machinery. However, problems have often arisen in obtaining high amounts of heterologous proteins in the culture medium. Here we present a quick method using sandwiched colonies to evaluate transgenic strains for secretion of heterologous proteins. Expressing the ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.5.2250-2252.1999
更新日期:1999-05-01 00:00:00
abstract::Cells of Bradyrhizobium japonicum were grown in media containing either 1.0 mM or 0.5 muM phosphorus. In growth pouch experiments, infection of the primary root of soybean (Glycine max (L.) Merr.) by B. japonicum USDA 31, 110, and 142 was significantly delayed when P-limited cells were applied to the root. In a greenh...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.10.2387-2392.1988
更新日期:1988-10-01 00:00:00